scholarly journals Long-term incubation with CXCL2, but not with CXCL1, alters the kinetics of TRPV1 receptors in cultured dorsal root ganglia neurons

2017 ◽  
Vol 69 (1) ◽  
pp. 53-59 ◽  
Author(s):  
Antonia-Teona Deftu ◽  
Alexandru-Florian Deftu ◽  
Violeta Ristoiu
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Ionic Channels ◽  
Homologous Sequence ◽  
Trigeminal Ganglia ◽  
Experimental Conditions ◽  
Trpv1 Receptors ◽  
Dorsal Root Ganglia Neurons ◽  
Kinetics Of

CXCL1 and CXCL2 are homologous chemokines that can be upregulated in different pathological conditions, affecting among other targets, neuronal ionic channels or receptors. TRPV1 is a polymodal nociceptor expressed in both dorsal root and trigeminal ganglia neurons. According to existing data, short-term incubation with CXCL1 can reduce TRPV1 desensitization, however, the long-term modulatory effect of both CXCL1 and CXCL2 on this receptor is less known. In the present study we investigated the influence of overnight incubation with 1.5 nM CXCL1 or CXCL2 on the functioning of TRPV1 receptors expressed in cultured dorsal root ganglia neurons. Calcium imaging and patch-clamp recordings showed that under the same experimental conditions and at the same concentration, only CXCL2 significantly decreased the TRPV1 current and increased its desensitization rate, whereas CXCL1 had no effect. This study proposes a different contribution of CXCL1 and CXCL2 to the modulation of TRPV1-mediated processes, in spite of their highly homologous sequence.

Characterization of A-425619 at native TRPV1 receptors: A comparison between dorsal root ganglia and trigeminal ganglia

2008 ◽  
Vol 596 (1-3) ◽  
pp. 62-69 ◽  
Author(s):  
Heath A. McDonald ◽  
Torben R. Neelands ◽  
Michael Kort ◽  
Ping Han ◽  
Melissa H. Vos ◽  
...  
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Trigeminal Ganglia ◽  
Trpv1 Receptors

scholarly journals Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons

2021 ◽  
Vol 22 (11) ◽  
pp. 5499
Author(s):  
Veronica Corsetti ◽  
Carla Perrone-Capano ◽  
Michael Sebastian Salazar Intriago ◽  
Elisabetta Botticelli ◽  
Giancarlo Poiana ◽  
...  
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Splice Variants ◽  
Multiple Roles ◽  
Pcr Analysis ◽  
Drg Neurons ◽  
Embryonic Developmental Stage ◽  
Embryo Stage ◽  
Dorsal Root Ganglia Neurons ◽  
Cholinergic Markers

Dorsal root ganglia (DRG) neurons synthesize acetylcholine (ACh), in addition to their peptidergic nature. They also release ACh and are cholinoceptive, as they express cholinergic receptors. During gangliogenesis, ACh plays an important role in neuronal differentiation, modulating neuritic outgrowth and neurospecific gene expression. Starting from these data, we studied the expression of choline acetyltransferase (ChAT) and vesicular ACh transporter (VAChT) expression in rat DRG neurons. ChAT and VAChT genes are arranged in a “cholinergic locus”, and several splice variants have been described. Using selective primers, we characterized splice variants of these cholinergic markers, demonstrating that rat DRGs express R1, R2, M, and N variants for ChAT and V1, V2, R1, and R2 splice variants for VAChT. Moreover, by RT-PCR analysis, we observed a progressive decrease in ChAT and VAChT transcripts from the late embryonic developmental stage (E18) to postnatal P2 and P15 and in the adult DRG. Interestingly, Western blot analyses and activity assays demonstrated that ChAT levels significantly increased during DRG ontogenesis. The modulated expression of different ChAT and VAChT splice variants during development suggests a possible differential regulation of cholinergic marker expression in sensory neurons and confirms multiple roles for ACh in DRG neurons, both in the embryo stage and postnatally.

Cryopreservation of transfected primary dorsal root ganglia neurons

2008 ◽  
Vol 173 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Angela M. Seggio ◽  
Karen S. Ellison ◽  
Matthew R. Hynd ◽  
William Shain ◽  
Deanna M. Thompson
Keyword(s):  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Dorsal Root Ganglia Neurons
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