scholarly journals Effect of salinity on Arabidopsis thaliana seed germination and acid phosphatase activity

2016 ◽  
Vol 68 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Nawel Nasri ◽  
Samira Maatallah ◽  
Rym Kaddour ◽  
Mokhtar Lachâal

The salt tolerance of four accessions of Arabidopsis thaliana (COL (Columbia), NOK2, N1438 and N1380) was evaluated during germination by the capacity of seeds to germinate in the presence of 50 mM NaCl and to maintain adequate acid phosphatase activity. Our results show that saline conditions reduced the final germination percentage, speed of germination and delayed the germination processes of accessions NOK2, N1438 and N1380. In contrast, 100% of germination was found in COL under salt-stress conditions. In the presence of NaCl 50 mM, acid phosphatase activity increased in the first 24 h, the activity reaching the control level in germinating seeds of COL, but in the three other accessions NOK2, N1438 and N1380, acid phosphatase activity diminished under salt stress. These findings suggest that changes in the phosphatase enzymes might play an important role in the acclimation of COL seeds to the changing environmental conditions.

2014 ◽  
Vol 58 (1) ◽  
pp. 47-57 ◽  
Author(s):  
Stanisław Flasiński ◽  
Ryszard Zamorski ◽  
Urszula Kotowska

Oilseed rape plants responded to water and salt stresses (-0.5 MPa, PEG 6000 and NaCI) by reduction of the fresh and dry weights of shoots and roots. When PEG was used, the ratio of dry weights of roots:shoots surpassed that of controls. The leaf protein content increased considerably. The phosphorus content decreased only in the roots, most significantly after three days of stress. Immediately after the stresses were induced, an increase in the acid phosphatase (AP) activity was noted. Water and salt stresses caused four- and two-fold increases in AP activity in leaves, respectively. Changes in the enzyme activity were negligible in stems and roots. There are nine forms of AP in young leaves of oilseed rape. In the stressed plants, from No. 5 revealed lower activity and forms Nos 8 and 9, higher activities than in the control. The increase in AP activity was directly accompanied by the decrease in the water potential of the tissues. Oilseed rape is considerably less sensitive to salt stress than to water stress, which is manifested as the lower inhibition of plant growth and also by a smaller increase in acid phosphatase activity.


2019 ◽  
Vol 17 (1) ◽  
pp. 39-44
Author(s):  
Md. Mozahidul Islam ◽  
Md. Sabibul Haque ◽  
AKM Golam Sarwar

Cowpea (Vigna unguiculata (L.) Walp.) is one of the most important grain legumes worldwide and its production is affected by increasing soil salinity due to global climate change. An experiment was conducted at the Plant Physiology Laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh to evaluate the germination capability of seven cowpea genotypes under salt stress. The germination test was carried out in Petri dishes following two factorial CRD with three replications. Seven cowpea genotypes viz, Red Pine, Green Super, Hai Jiang San Hao, Kegornatki, Kegornatki Green, Kegornatki HYV & Kegornatki Red; and three salt levels viz, 0, 6 & 12 dSm-1, were used as experimental treatments. The germination percentage (GP), mean germination time (MGT), radicle and plumule length, radicle and plumule fresh and dry weight and different stress tolerance indices were recorded to screen the genotypes for salt tolerance. The study highlighted that salt concentrations drastically reduced seed germination and significantly delayed the process in all genotypes. The GP, length and biomass of radicle and plumule and salt tolerance indices were significantly decreased while the MGT was significantly increased with increasing salt stress in all cowpea genotypes. A significant variation among the genotypes in response to salt stress was also observed. Among the seven cowpea genotypes, Hai Jiang San Hao and Green Super showed higher salt tolerance in comparison to the other genotypes based on the measured traits; these genotypes can be used for further breeding program and/or cultivation in coastal saline prone areas with further investigation. J. Bangladesh Agril. Univ. 17(1): 39–44, March 2019


Author(s):  
O. T. Minick ◽  
E. Orfei ◽  
F. Volini ◽  
G. Kent

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).


2008 ◽  
Vol 39 (6) ◽  
pp. 627-634 ◽  
Author(s):  
Tatiana Salles de Souza Malaspina ◽  
Célio Xavier dos Santos ◽  
Ana Paula Campanelli ◽  
Francisco Rafael Martins Laurindo ◽  
Mari Cleide Sogayar ◽  
...  

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