scholarly journals DNA insecticides based on iap3 gene fragments of cabbage looper and gypsy moth nuclear polyhedrosis viruses show selectivity for non-target insects

2015 ◽  
Vol 67 (3) ◽  
pp. 785-792 ◽  
Author(s):  
Volodymyrovych Oberemok ◽  
Volodymyrivna Laikova ◽  
Sergeevich Zaitsev ◽  
Mutah Nyadar ◽  
Nikolaevich Shumskykh ◽  
...  
Keyword(s):  
Lymantria Dispar ◽  
Trichoplusia Ni ◽  
Insect Pest ◽  
Cabbage Looper ◽  
Black Cutworm ◽  
Insect Pest Control ◽  
Insecticidal Effect ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Potential Use

DNA insecticides are novel preparations based on short single-stranded fragments of anti-apoptotic (IAP) genes of nuclear polyhedrosis viruses. In this article we report about the harmlessness of the DNA insecticides based on single-stranded fragments of the LdMNPV (Lymantria dispar multiple nucleocapsid polyhedrosis virus) IAP3 gene for tobacco hornworm and black cutworm, and present the first evidence of significant insect-specific insecticidal effect of single-stranded TnSNPV (Trichoplusia ni single nucleocapsid polyhedrosis virus) IAP3 gene fragments on the viability of cabbage looper and their harmlessness for black cutworm and wheat weevil. Our results show that DNA insecticides based on LdMNPV IAP3 and TnSNPV IAP3 gene fragments can be selective in action and have potential use in insect pest control.

THE PERSISTENCE OF A NUCLEAR POLYHEDROSIS VIRUS IN THE HABITAT OF THE HOST INSECT, TRICHOPLUSIA NI: I. POLYHEDRA DEPOSITED ON FOLIAGE

1967 ◽  
Vol 99 (8) ◽  
pp. 785-794 ◽  
Author(s):  
R. P. Jaques
Keyword(s):  
Ultraviolet Light ◽  
Trichoplusia Ni ◽  
Laboratory Tests ◽  
Nuclear Polyhedrosis Virus ◽  
Cabbage Looper ◽  
Host Insect ◽  
Aqueous Suspensions ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

AbstractField, glasshouse, and laboratory tests showed that deposits of polyhedra of the nuclear polyhedrosis virus of the cabbage looper, Trichoplusia ni (Hübner), on foliage are practically non-infective after a 1-month exposure to weathering. Inactivation by sunlight appeared to be more important than removal by washing by rain in causing this loss of activity. The virus was readily inactivated by exposure to ultraviolet light. Virus exposed in aqueous suspensions of polyhedra or in wetted deposits was inactivated by shorter exposures than was virus in dry deposits of polyhedra.

scholarly journals Characterization and serology of the matrix protein from a nuclear-polyhedrosis virus of Trichoplusia ni before and after degradation by an endogenous proteinase

1977 ◽  
Vol 167 (2) ◽  
pp. 321-332 ◽  
Author(s):  
D A Eppstein ◽  
J A Thoma
Keyword(s):  
Trichoplusia Ni ◽  
Matrix Protein ◽  
Nuclear Polyhedrosis Virus ◽  
Monomer Unit ◽  
Matrix Proteins ◽  
Cabbage Looper ◽  
Before And After ◽  
The Matrix ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

The intact matrix protein from a nuclear-polyhedrosis virus of the cabbage looper (Trichoplusia ni), isolated after inhibition of an endogenous serine-type proteinase, was further purified by molecular-sieve chromatography. The matrix protein was associated with carbohydrate moieties, and the carbohydrate content was determined for the two major peptides isolated after proteolysis by the endogenous proteinase. The association-dissociation interactions of the intact and proteinase-hydrolysed monomer units were characterized at high and low pH. At pH1.9, proteinase-degraded matrix protein dissociated into two different peptide fractions, FI and FII. Fraction FII, a single peptide of 9400 daltons, comprised one-third of the monomer unit of 28 000 daltons. At pH9.5, the degraded peptides were tightly associated in units equivalent to the intact monomer. These monomer equivalents associated to form a series of interconverting aggregates. The predominant aggregate sedimented at 11S and had a mol.wt greater than or equal to 200 000. Two non-cross-reacting antigens were present in the aggregate mixture. The presence of these two antigens does not reflect the presence of two different matrix proteins; rather, the expression of the antigens correlates with the degree of aggregation of the matrix protein.

NATURAL OCCURRENCE OF VIRUSES OF THE CABBAGE LOOPER IN FIELD PLOTS

1970 ◽  
Vol 102 (1) ◽  
pp. 36-41 ◽  
Author(s):  
R. P. Jaques
Keyword(s):  
Trichoplusia Ni ◽  
Nuclear Polyhedrosis Virus ◽  
Cabbage Looper ◽  
Natural Occurrence ◽  
Virus Diseases ◽  
Granulosis Virus ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Cruciferous Plants ◽  
Field Plots

AbstractStudies on non-treated plots at Harrow, Ontario, in 1967 and 1968 and at Kentville, Nova Scotia, from 1963 to 1968 inclusive showed that residues of viruses of the cabbage looper, Trichoplusia ni (Hübner), found in soil were related qualitatively and quantitatively to the virus residues on leaves of cruciferous plants grown in the plots and to mortality by viruses in the host populations. At Harrow where the cabbage looper is common and where virus diseases occur naturally in field populations, the viruses that occurred in the populations of the host built up in soil as the season progressed. At Kentville where the host is rare, virus was rarely found in soil or on foliage. The typical nuclear-polyhedrosis virus, an atypical nuclear-polyhedrosis virus that formed abnormally large polyhedra, and a granulosis virus were found at Harrow, with the typical nuclear-polyhedrosis virus predominating. Only the typical nuclear-polyhedrosis virus was detected at Kentville.

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