scholarly journals The therapeutic potential of propolis against damage caused by Salmonella typhimurium in mice liver: A biochemical and histological study

2015 ◽  
Vol 67 (3) ◽  
pp. 807-816 ◽  
Author(s):  
Preeti Kalia ◽  
Neelima Kumar ◽  
Kusum Harjai
Keyword(s):  
Salmonella Typhimurium ◽  
Therapeutic Potential ◽  
Histological Study ◽  
Experimental Period ◽  
Hepatoprotective Activity ◽  
Bacterial Diseases ◽  
Colony Forming Units ◽  
Mice Liver ◽  
Limited Scope ◽  
Different Doses

Honeybee products are a rich source of nutritive supplements and traditional medication. The increasing resistance of bacteria towards various antibiotics and the limited scope of some vaccines makes it important to explore alternative therapies to combat bacterial diseases. The present study aimed to evaluate the antibacterial action of propolis using biochemical and histopathological methods in Salmonella typhimurium-infected BALB/c mice. Crude propolis was collected from an apiary and extracted with 70% ethanol. Hepatotoxicity was induced in mice by infecting them with Salmonella typhimurium (104 colony-forming units (CFU)), and the hepatoprotective activity of propolis was evaluated by administration of different doses of propolis (100, 300 and 500 mg/kg body weight) for 30 days. Biochemical and histopathological examinations were performed at regular intervals during the experimental period. Results obtained after treatment were compared with similar studies performed on normal control mice. Infected mice showed elevated liver marker enzymes and revealed the presence of characteristic typhoidal nodules in histological preparations. These results point to the therapeutic activity of propolis against Salmonella typhimurium.

Inhibition of Salmonella typhimurium on Agar Medium and Poultry Skin by Ultraviolet Energy

1996 ◽  
Vol 59 (3) ◽  
pp. 319-321 ◽  
Author(s):  
SUSAN S. SUMNER ◽  
EVA A. WALLNER-PENDLETON ◽  
GLENN W. FRONING ◽  
LA VERNE E. STETSON
Keyword(s):  
Ultraviolet Radiation ◽  
Salmonella Typhimurium ◽  
Agar Medium ◽  
Uv Light ◽  
Light Treatment ◽  
Complete Elimination ◽  
Bacterial Reduction ◽  
Colony Forming Units ◽  
Ultraviolet Energy ◽  
Different Doses

Ultraviolet radiation (UV) was effective in destroying Salmonella typhimurium on agar plates and poultry skin. Agar plates inoculated with varying numbers of colony-forming units (CFU) of S. typhimurium (1.2 × 102 to 1.7 × 109) were subjected to different doses of UV light to determine optimal killing. Poultry skin was also inoculated with varying CFU of S. typhimurium per 2 cm2 of skin and subjected to UV light. UV light treatment of inoculated agar plates revealed almost complete elimination (99.9%) of S. typhimurium at 2,000 μW · s · cm−2. Bacterial reduction was less effective on the surface of poultry skin when a 80.5% reduction in S. typhimurium was obtained at 2,000 μW · s · cm−2.

scholarly journals Investigation of Pharmacological Activity of Caralluma penicillata: Anti-Inflammatory Properties and Gastritis Protection against Indomethacin in Adult Guinea Pigs

2014 ◽  
Vol 2014 ◽  
pp. 1-9
Author(s):  
Nabil Albaser ◽  
Najeeb Ghanem ◽  
Mohanad Shehab ◽  
Adnan Al-Adhal ◽  
Mohammed Amood AL-Kamarany
Keyword(s):  
Chronic Inflammation ◽  
Guinea Pigs ◽  
Therapeutic Potential ◽  
Histological Study ◽  
Folk Medicine ◽  
Ethanol Extract ◽  
Optimum Dose ◽  
Repeated Doses ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Caralluma is a plant that possessing a great therapeutic potential in folk medicine in Yemen, namely, Caralluma penicillata (C. penicillata) as antiulcer. The study aims to evaluate the anti-inflammatory properties and gastritis protection activity of C. penicillata against indomethacin in adult guinea pigs. The study was divided into four parts: firstly, the optimum dose of extract as anti-inflammatory effect was determined. Secondly, the acute anti-inflammatory effect of extract were estimated. Thirdly, the repeated doses of extract against chronic inflammation was estimated. The anti-inflammatory activity of extract was compared with indomethacin as a prototype of drug against inflammation. Fourthly, the gastritis protection properties of extract with/without indomethacin were performed. The results showed that a 400 mg/kg of 10% ethanol extract produced the maximum of anti-inflammatory effect. Also, the single dose of extract was equipotent for indomethacin (10 mg/kg), but shorter in duration with regard to acute anti-inflammatory effect. In addition, the repeated doses of extract against chronic inflammation were less potent than indomethacin with regard to ulcerogenic effect. On the other hand, extract-indomethacin combination reduced the gastritis effect of indomethacin based on ulcer index and histological study.

scholarly journals Therapeutic Potential of Human Neutrophil Peptide 1 against Experimental Tuberculosis

2001 ◽  
Vol 45 (2) ◽  
pp. 639-640 ◽  
Author(s):  
Sudhir Sharma ◽  
Indu Verma ◽  
G. K. Khuller
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Human Neutrophil ◽  
Therapeutic Efficacy ◽  
Therapeutic Potential ◽  
Therapeutic Activity ◽  
Experimental Tuberculosis ◽  
Dose Dependent ◽  
Different Doses ◽  
Human Neutrophil Peptide

ABSTRACT The therapeutic efficacy of human neutrophil peptide 1 (HNP-1) against experimental tuberculosis in mice on the basis of numbers of CFU has been examined. Mice infected with 1.5 × 104CFU of Mycobacterium tuberculosisH37Rv and treated with different doses of HNP-1 injected subcutaneously exhibited significant clearance of bacilli from lungs, livers, and spleens. There were time- and dose-dependent decreases in the bacillary load in lungs, livers, and spleens of the HNP-1-treated animals compared to that in controls (untreated animals). These observations strongly suggest the therapeutic activity of HNP-1 against tuberculosis.

Preliminary Study on the Anti-radiation Effect of Jen-Sheng-Yang-Yung-Tang

1993 ◽  
Vol 21 (02) ◽  
pp. 187-195 ◽  
Author(s):  
Hsue-yin Hsu ◽  
Yau-hui Ho ◽  
Shi-Iong Lian ◽  
Chun-ching Lin
Keyword(s):  
Bone Marrow ◽  
Radiation Effect ◽  
Bone Marrow Cells ◽  
X Rays ◽  
Male Mice ◽  
X Ray ◽  
Colony Forming Units ◽  
Before And After ◽  
Preliminary Study ◽  
Different Doses

Six to seven week old male mice of ICR strain were exposed to different doses of x-rays to determine if Jen-Sheng-Yang-Yung-Tang could be a modifier in the elimination of radiation damage. Colony forming units of bone marrow cells in the spleen (CFUs) were measured before and after x-ray irradiation with intraperitoneal injection of 10 mg/20 g or 20 mg/20 g body weight of Jen-Sheng-Yang-Yung-Tang, once a day for seven consecutive days. The recovery of CFUs and hemocytes counts by 4 Gy irradiation with Jen-Sheng-Yang-Yung-Tang administration was faster for a concentration of 20 mg/20 g than 10 mg/20 g. The measurement of 10-day CFUs showed an increase of radiotolerance in the treatment of 20 mg/20 g administration before x-ray irradiation. The injection of Jen-Sheng-Yang-Yung-Tang accelerated the recovery of hemocyte counts in mice irradiated with 4 Gy x-ray; the effect was especially profound for leukocytes with 20 mg/20 g Jen-Sheng-Yang-Yung-Tang administration after irradiation.

Evaluation of Hepatoprotective activity of Methanol extract of Persicaria hydropiper: An in vivo screening

2021 ◽  
pp. 5819-5824
Author(s):  
Pooja Kamra ◽  
Mahaveer Singh ◽  
Hardarshan Singh Lamba ◽  
Birendra Srivastava
Keyword(s):  
Carbon Tetrachloride ◽  
Intraperitoneal Administration ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Standard Drug ◽  
Whole Plant ◽  
Dose Dependent ◽  
Histopathological Investigation ◽  
Different Doses

The present study aimed to evaluate the hepatoprotective potential of methanolic whole plant extract of Persicaria hydropiper in carbon tetrachloride (CCl4) induced hepatotoxicity model. Hepatotoxicity was induced in rats by intraperitoneal administration of carbon tetrachloride (CCl4) for seven days. The extract was thereafter administered at two different doses of 200 mg/kg and 400 mg/kg body weight for next seven days. Silymarin was used as a reference standard. The extract revealed hepatoprotective activity in dose dependent manner. The dose of 400 mg/kg exhibited maximum hepatoprotective ability as apparent from several evaluation parameters including liver function profile, bilirubin, antioxidant enzymes as well as histopathological investigation which was comparable to the standard drug Silymarin respectively. These findings sustenance the use of the extract as an adjuvant with existing therapy for treatment of liver ailments.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF CORDIA OBLIQUA AGAINST PARACETAMOL-INDUCED LIVER TOXICITY ON WISTAR RATS

2019 ◽  
pp. 149-152
Author(s):  
SIVAKRISHNAN SIVAGNANAM ◽  
PRADEEPRAJ DEVARASU
Keyword(s):  
Wistar Rats ◽  
Liver Toxicity ◽  
Histological Study ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Plant Materials ◽  
Percolation Method ◽  
Soxhlet Apparatus ◽  
Serum Glutamate Pyruvate Transaminase

Objective: The ethanolic extract of Cordia obliqua (EECO) Willd. leaves was evaluated for hepatoprotective activity in Wistar rats by inducing hepatic damage with paracetamol. Methods: The leaves of C. obliqua were dried under shade, segregated, pulverized by a mechanical grinder and passed through a 40 mesh sieve. The powdered plant materials were stored in an air-tight container. The above-powdered materials were successively extracted with ethanol by hot continuous percolation method in Soxhlet apparatus for 24 h. The extract was concentrated using a rotary evaporator and subjected to freeze drying in a lyophilizer till dry powder was obtained. Results: Treatment with EECO at 200 and 400 mg/kg showed significant decrease in serum glutamate-pyruvate transaminase, serum glutamic oxaloacetic transaminase, alkaline phosphatase, and total bilirubin levels and a significant elevation in the total protein, albumin, and globulin levels in serum when compared with paracetamol treated rats. In histological study of liver, the hepatocytes show moderate cytoplasm and moderately enlarged pleomorphic and hyperchromatic nuclei. The portal triads show mild periportal inflammation composed of lymphocytes, and central veins are normal. Conclusion: On the basis of results, we can conclude that the EECO leaves showed potential hepatoprotective activity in rats.

scholarly journals PENGARUH PEMBERIAN TUAK TERHADAP GAMBARAN HISTOPATOLOGI HATI MENCIT (Mus musculus) ICR JANTAN

2020 ◽  
Vol 11 (2) ◽  
pp. 193
Author(s):  
St Aisyah Sijid ◽  
Cut Muthiadin ◽  
Zulkarnain Zulkarnain ◽  
Ar. Syarif Hidayat
Keyword(s):  
Body Weight ◽  
Liver Disease ◽  
Liver Damage ◽  
Mus Musculus ◽  
Liver Weight ◽  
Palm Wine ◽  
Icr Mice ◽  
Liver Histopathology ◽  
Mice Liver ◽  
Different Doses

Liver damage or liver disease is caused by many factors, one of which is by consuming drinks that contain alcohol. Tuak is one of the drinks that contain alcohol. This study aims to determine the effect of palm wine administration on the histopathological picture of male ICR mice (Mus musculus) liver. This study used 20 mice consisting of 4 treatments namely P0 = 0 mL / day / head; P1 = 0.1 mL / day / head; P2 = 0.2 mL / day / head and P3 = 0.3 mL / day / head. The parameters observed were body weight of mice, liver weight and liver histopathology of mice. The results showed that administration of palm wine to male ICR mice at different doses gave an influence on the histopathological picture of male ICR mice (Mus musculus).AbstrakKerusakan hati atau penyakit liver disebabkan oleh banyak faktor, salah satunya adalah dengan mengkonsumsi minuman yang mengandung alkohol. Tuak merupakan salah satu minuman yang mengandung alkohol. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tuak terhadap gambaran histopatologi hati mencit (Mus musculus) ICR jantan. Penelitian ini menggunakan 20 ekor mencit yang terdiri dari 4 perlakuan yaitu P0 = 0 mL/hari/ekor; P1 = 0,1 mL/hari/ekor; P2 = 0,2 mL/hari/ekor dan P3 = 0,3 mL/hari/ekor. Parameter yang diamati adalah berat badan mencit, berat hati dan histopatologi hati mencit. Hasil penelitian menunjukkan bahwa pemberian tuak pada mencit ICR jantan dengan dosis yang berbeda memberikan pengaruh terhadap gambaran histopatologi hati mencit (Mus musculus) ICR jantan.Kata Kunci: Hati, Histopatologi, Mencit, Tuak

scholarly journals Hepatoprotective Activity of Curcuma mangga Extract on Paracetamol-Induced Male Mice

2018 ◽  
Vol 1 (2) ◽  
pp. 35-40
Author(s):  
Yuandani ◽  
Silvia Mardaliza ◽  
Marianne
Keyword(s):  
Ethanol Extract ◽  
Serum Levels ◽  
Hepatic Necrosis ◽  
Hepatoprotective Activity ◽  
Negative Control ◽  
Hepatoprotective Effect ◽  
Hepatic Tissue ◽  
High Dose ◽  
Male Mice ◽  
Mice Liver

This study was carried out to investigate the protective effect of ethanol extract of Curcuma mangga rhizomes on paracetamol-induced hepatotoxicity. High dose of paracetamol (1.35g/kg bw) was used to induce hepatic necrosis of mice liver. The male mice  received ethanol extract of C. mangga rhizomes (100, 200 and 400 mg/kg BW) for 7 days. The hepatoprotective actvity of extract was compared to normal, positive (curcuma) and negative control. The liver function was evaluated by measuring the biochemistry parameters which include alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, histophatological study on hepatic tissue section was also carried out. The C. mangga extract displayed hepatoprotective effect except at dose of 100 mg/kg bw. The increasing of serum levels of AST and ALT were inhibited after treatment with ethanol extract at doses of 200 and 400 mg/kb bw which was comparable with normal and curcuma as postive control (p>0.05). In addition, histological assessment of hepatic tissue demonstrated no liver damage, specially at dose of 400 mg/kb BW. The result indicate that ethanol extract of C. mangga rhizomes has hepatoprotective effect, especially at doses of 200 and 400 mg/kg bw .   Keywords: C. mangga, rhizomes, biochemistry parameters, histopathology

scholarly journals Hepatoprotective activity of aqueous extract from Caragana jubata (Pall.) Poir. shoots in the model of acute hepatitis induced by acetaminophen in rats

2018 ◽  
Vol 64 (3) ◽  
pp. 241-246 ◽  
Author(s):  
P.A. Kakorin ◽  
I.V. Babenkova ◽  
Y.O. Teselkin ◽  
G.V. Ramenskaya ◽  
T.A. Demura ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Alkaline Phosphatase ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Total Bilirubin ◽  
Histological Study ◽  
Blood Parameters ◽  
Hepatoprotective Activity ◽  
Reference Preparation ◽  
Liver Homogenates

The aim of the study was to investigate the hepatoprotective activity of an aqueous extract of Caragana jubata (Pall.) Poir. Acute experimental hepatitis was induced by acetaminophen administration of 1000 mg/kg. Studies were conducted in white Wistar rats. The aqueous extract of C. jubata demonstrated the hepatoprotective effect, comparable to that of the reference preparation “Carcil”. This was manifested by the normalization of biochemical blood parameters (ALT, AST, alkaline phosphatase, cholesterol, total bilirubin) and antioxidant activity of liver homogenates, determined by the method based on oxidation of luminol induced by 2,2¢-azo-bis-2-amidinopropane. Normalization of morphofunctional indices was also shown in a histological study of liver of rats that received aqueous extract from C. jubata.

scholarly journals An Overview of Some Plant Based Products With Hepatoprotective Activity

2021 ◽  
Vol 30 (2) ◽  
pp. 1-15
Author(s):  
Sonali Dnyaneshwar Labhade ◽  
Swapnil Sharma ◽  
Sarvesh Paliwal ◽  
Shivani Desai
Keyword(s):  
Medicinal Plants ◽  
Therapeutic Potential ◽  
Folk Medicine ◽  
Herbal Medicines ◽  
Repeated Administration ◽  
Hepatoprotective Activity ◽  
Hepatoprotective Effect ◽  
Quality Trait ◽  
Liver Disorders ◽  
Current Review

In folk medicine there are various medicinal amalgamation possessing hepatoprotective activity. This activity is of significance because several toxins cause liver injury. Hence, many pharmaceutical companies are targeting herbal medicines for the treatment of liver abnormalities and towards evolving a safe and effective formulation with desired route of administration. In current review we have focused on the studies showing hepatoprotective effect using marine compounds and plant derived compounds. Liver disorder, a global health problem, usually include acute or chronic hepatitis, heptoses, and cirrhosis. It may be due to toxic chemicals and certain antibiotics. Uncontrolled consumption of alcohol also affects liver in an unhealthy way. To cure liver disorders several formulations of medicinal plants are being used. It is observed that hepatoprotective effect of plant is mostly due to flavonoids, alkaloids, terpenoids, steroids, and glycoside. A single drug cannot be useful for all the types of liver disorders. Several plant extracts for liver illness results from poisonous chemicals, viruses, extra alcohol consumption, and repeated administration of medication. By using standards of protection and efficacy, manufacture of plant products need to be ruled out. Current review provides an understanding of ethnopharmocology, toxicology of several medicinal plants manifesting hepatoprotective potential. Despite of varied database analysis new discoveries and their probabilities, evidences on viral hepatitis treatment or liver cirrhosis is inadequate.  Further information about phytotherapy, toxicology, quality control studies shall be endorsed. Further in depth studies are required to discover quality trait like SAR, MOA, safety and toxicity and therapeutic potential of phytoconstituents in clinical settings.

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