scholarly journals Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia

2014 ◽  
Vol 66 (2) ◽  
pp. 683-690 ◽  
Author(s):  
Sanja Cakic ◽  
Miljana Mojsilovic ◽  
D. Mihaljica ◽  
Marija Milutinovic ◽  
A. Petrovic ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Genetic Variability ◽  
Ixodes Ricinus ◽  
Intraspecific Variability ◽  
Balkan Peninsula ◽  
Coi Gene ◽  
Gene Sequences ◽  
Ricinus Tick ◽  
Pathogenic Agents

The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas.

scholarly journals Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia

2014 ◽  
Vol 66 (3) ◽  
pp. 1243-1251
Author(s):  
Sanja Cakic ◽  
Miljana Mojsilovic ◽  
Darko Mihaljica ◽  
Marija Milutinovic ◽  
Andjeljko Petrovic ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Ixodes Ricinus ◽  
Intraspecific Variability ◽  
Balkan Peninsula ◽  
Coi Gene ◽  
Gene Sequences ◽  
Ricinus Tick ◽  
Pathogenic Agents ◽  
Font Color

The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas. [Projekat Ministarstva nauke Republike Srbije, br. ON 173006] <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href='http://dx.doi.org/10.2298/ABS1404689U'>10.2298/ABS1404689U</a><u></b></font>

scholarly journals The genomic Echinococcus microsatellite EmsB sequences: from a molecular marker to the epidemiological tool

Parasitology ◽  
2009 ◽  
Vol 137 (3) ◽  
pp. 439-449 ◽  
Author(s):  
J. KNAPP ◽  
J. M. BART ◽  
S. MAILLARD ◽  
B. GOTTSTEIN ◽  
R. PIARROUX
Keyword(s):  
Genetic Structure ◽  
Genetic Variability ◽  
Molecular Marker ◽  
Alveolar Echinococcosis ◽  
Adaptive Strategies ◽  
Parasitic Disease ◽  
The Past ◽  
Epidemiological Tool ◽  
Important Challenge

SUMMARYIn the field of molecular and epidemiological parasitology, characterization of fast evolving genetic markers appears as an important challenge to consider the diversity and genetic structure of parasites. The study of respective populations can help us to understand their adaptive strategies to survive and perpetuate the species within different host populations, all trying to resist infection. In the past, the relative monomorphic features of Echinococcus multilocularis, the causative agent of alveolar echinococcosis and a severe human parasitic disease, did not stimulate studies dealing with the genetic variability of Echinococcus species or respective populations. A recently developed, characterized and validated original multilocus microsatellite, named EmsB, tandemly repeated in the genome, offered an additional opportunity for this line of investigation. We have compiled in this review new insights brought by this molecular tracker on the transmission activity of Echinococcus among different hosts and at different geographical scales.

scholarly journals Limited Genetic Structure of Gypsy Moth Populations Reflecting a Recent History in Europe

Insects ◽  
2018 ◽  
Vol 9 (4) ◽  
pp. 143 ◽  
Author(s):  
Nikola Lacković ◽  
Milan Pernek ◽  
Coralie Bertheau ◽  
Damjan Franjević ◽  
Christian Stauffer ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Gypsy Moth ◽  
Phylogenetic Reconstruction ◽  
Population Expansion ◽  
Balkan Peninsula ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Low Genetic Diversity ◽  
Genetic Structure And Diversity

The gypsy moth, Lymantria dispar, a prominent polyphagous species native to Eurasia, causes severe impacts in deciduous forests during irregular periodical outbreaks. This study aimed to describe the genetic structure and diversity among European gypsy moth populations. Analysis of about 500 individuals using a partial region of the mitochondrial COI gene, L. dispar was characterized by low genetic diversity, limited population structure, and strong evidence that all extant haplogroups arose via a single Holocene population expansion event. Overall 60 haplotypes connected to a single parsimony network were detected and genetic diversity was highest for the coastal populations Croatia, Italy, and France, while lowest in continental populations. Phylogenetic reconstruction resulted in three groups that were geographically located in Central Europe, Dinaric Alps, and the Balkan Peninsula. In addition to recent events, the genetic structure reflects strong gene flow and the ability of gypsy moth to feed on about 400 deciduous and conifer species. Distinct genetic groups were detected in populations from Georgia. This remote population exhibited haplotypes intermediate to the European L. dispar dispar, Asian L. dispar asiatica, and L. dispar japonica clusters, highlighting this area as a possible hybridization zone of this species for future studies applying genomic approaches.

scholarly journals Mitochondrial Gene Sequence (COI) Reveals the Genetic Structure and Demographic History of Lymantria dispar (Lepidoptera: Erebidae: Lymantriinae) in and around China

Insects ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 146 ◽  
Author(s):  
Yao Xu ◽  
Sufang Zhang ◽  
Hongbin Wang ◽  
Mei Wang ◽  
Guohong Li
Keyword(s):  
Genetic Structure ◽  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Demographic History ◽  
Mitochondrial Gene ◽  
Dna Barcode ◽  
Full Length ◽  
Coi Gene ◽  
Gene Sequences ◽  
Gypsy Moths

The gypsy moth, Lymantria dispar, is among the most destructive quarantine pests of forests. Here, we reconstructed the genetic structure and determined the population differentiation of gypsy moths across its distribution range at different times. This information could be used to both improve the prevention and detection of gypsy moths in the field. Using 31 newly designed species-specific primers targeting fragments of 216–1102 bp, we identified 103 full-length cytochrome oxidase subunit I (COI) gene sequences from eight fresh samples and 95 L. dispar specimens collected between 1955 and 1996, mainly in China. Combining 103 full-length COI gene sequences with 146 COI gene sequences from Genbank or DNA barcode libraries, we analyzed the genetic differentiation, gene flow and haplotypes between gypsy moth populations in order to reflect the genetic structure and population dynamics of gypsy moths. We discovered 25 previously unknown haplotypes from old gypsy moth specimens. We found that the genetic diversity among gypsy moth populations (collected in the same region at different time points) was relatively high. Furthermore, the genetic structure of Chinese geographical populations (Heilongjiang, Liaoning, Beijing) in different years was distinct. Our results suggested that some gypsy moths in China showed the genetic affinity with European gypsy moths (a sub-species of gypsy moths found mainly in Europe).

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