scholarly journals Cografts of artificial dermis matrix and autogenetic split-thickness of repaired skin in severe hand wounds in patients with deep burns

2014 ◽  
Vol 66 (1) ◽  
pp. 173-178
Author(s):  
Hongqi Liu ◽  
Yan Li ◽  
Deqian Sha
Keyword(s):  
Wound Healing ◽  
Hand Function ◽  
Donor Site ◽  
Test Group ◽  
Healing Time ◽  
Whole Body ◽  
Control Group ◽  
Large Area ◽  
Artificial Dermis ◽  
Thickness Skin

The aim of this paper was to evaluate the effect of using artificial dermis matrix plus autologous split-thickness skin (ADM and ASTS) in the treatment of deep-burns in hands of severely burned patients?We recruited a total of 58 patients with large area burns greater than 80% that were eschar-excised. Twenty-eight of them were treated with ADM and ASTS (test group); 30 were treated with autologous medium-thickness skin (AMTS) (control group). The healing time of the hand wound was noted, clinical and photographic evaluations were performed, and a Jebsen-Taylor hand function test was compared and analyzed in the two groups. The wound healing time in the test group (24.22?3.34 days) were longer than that of the control group (13.42?3.36 days) and statistically significant. The healing time of skin graft donor sites was shorter than that of the control group (7.14?1.63 vs. 14.28?2.37 days) and statistically significant (P<0.05). The 3rd and 6th month follow-up with clinical and functional evaluations revealed no differences between the two groups. In addition, there was no obvious scar formation and less pigmentation in either group. The repair of deeply burned hands with artificial dermis matrix was beneficial to both wound healing and the donor site, and was beneficial to the whole body rehabilitation of severely burned patients.

Electrical stimulation on open wound healing in Rabbits

2018 ◽  
Vol 33 (2) ◽  
pp. 51-61
Author(s):  
MH Mia ◽  
MM Hossain ◽  
UK Rima ◽  
MM Rahman ◽  
MR Alam ◽  
...  
Keyword(s):  
Wound Healing ◽  
Electrical Stimulation ◽  
Healing Process ◽  
Skin Incision ◽  
Healing Time ◽  
Skin Wound ◽  
Control Group ◽  
Thickness Skin ◽  
Experimental Group ◽  
Made In

Micro-amperage electrical stimulation (MES) on the healing process of skin wound in rabbits was studied. Twenty adult New Zealand white rabbits were randomly divided into four equal groups and a full thickness skin incision was made in each rabbit. This experimental group received an MES of 1000 μA (1mA) current intensity for 30 minutes twice a day. Swelling area of the wound of experimental groups were 11.1cd ± 0.2 mm, 10.8d ± 0.1 mm and 10.7cd ± 0.1mm at days 4, 7 and15, respectively. Elevation of sutured line (mm) of experimental groups was 2.4b ± 0.1, 2.1b ± 0.1, 2.2b ± 0.1 at days 4, 7 and 15, respectively. The average healing time was 30.4b, 28.4b and 26.4b in days 4, 7 and 15, respectively, significantly different from control groups. Number of fibroblasts and blood vessels were significantly higher in the experimental group than in control group. The result indicates that the application of MES significantly enhances the wound healing in rabbits.Bangl. vet. 2016. Vol. 33, No. 2, 51-61

Use of autologous platelet-rich plasma in healing skin graft donor sites

2020 ◽  
Vol 29 (1) ◽  
pp. 36-41
Author(s):  
Igor Slaninka ◽  
Aleš Fibír ◽  
Milan Kaška ◽  
Jiří Páral
Keyword(s):  
Wound Healing ◽  
Skin Graft ◽  
Platelet Rich Plasma ◽  
Donor Site ◽  
High Risk Patient ◽  
Healing Time ◽  
Control Group ◽  
Graft Donor ◽  
Time Required ◽  
Wound Healing Time

Objective: To demonstrate that the use of platelet-rich plasma (PRP) enhances both the quality of healing and the time required for wound healing at a skin graft donor site. Methods: Patients who had dermo-epidermal skin grafts taken from the thigh area were included in a prospective, randomised clinical study. PRP was applied to one donor site and then covered with Vaseline-impregnated, open-weave gauze and gauze, while the contralateral donor site on the other thigh served as a control and was covered with the open-weave gauze and gauze without PRP. Results: A total of 24 patients took part in the study, of which three developed infections and were thus removed from the study. Use of PRP reduced the wound healing time of the dermo-epidermal graft donor sites by a mean 17.8% and median 18 days. On average, the treated donor sites healed in 14.9 days compared with 18.4 days for the control group. The median was 14 days compared with 18 days in the control group (p=0.026). In one patient, healing was slower on the side where PRP was applied. In 20 patients, healing of the donor site was accelerated where PRP was applied. Conclusion: The study demonstrated a beneficial effect of PRP, as healing time was shortened. Using PRP to heal wounds could be beneficial for patients for whom commonly available wound healing therapies have failed, as well as for high-risk patient groups for whom problematic wound healing may be expected.

scholarly journals Free skin grafting in reconstructive surgery of burns in children

2017 ◽  
Vol 5 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Konstantin A. Afonichev ◽  
Maksim S. Nikitin ◽  
Yaroslav N. Proshchenko
Keyword(s):  
Reconstructive Surgery ◽  
Donor Site ◽  
Skin Grafting ◽  
Expansion Method ◽  
Control Group ◽  
Hypertrophic Scars ◽  
Large Area ◽  
Skin Expansion ◽  
Thickness Skin ◽  
Experimental Group

When performing reconstructive surgery in children suffering from extensive post-burn hypertrophic scars, the main problem is deficiency of donor intact skin. Aim. This study aimed to determine the possibility of using the expander skin balloon expansion method for obtaining free, large area split-thickness skin autografts. Materials and methods. A comparative analysis of treatment for 39 children with extensive post-burn hypertrophic scars was performed. In 16 children (experimental group), balloon skin expansion of a donor site for obtaining large area split-thickness skin grafts (more than 100 cm²) was performed. In 23 children (control group), the large area grafts were cut off without prior balloon skin expansion of the donor site. Results. In cases where it is necessary to close a wound defect over 100 cm², it is advisable to perform prior balloon skin expansion of the donor site. This technique enables attainment of an injury-resistant free implant full grafting material and also provides multiple uses of a donor site without disturbing the esthetics.

PENGGUNAAN SELAPUT AMNION SEGAR PADA INSISI LUKA OPERASI SEKSIO SESAREA

2019 ◽  
Vol 1 (2) ◽  
pp. 47-55
Author(s):  
Syahredi Syaiful Adnani ◽  
Hafni Bachtiar
Keyword(s):  
Wound Healing ◽  
Caesarean Section ◽  
Amniotic Membrane ◽  
Healing Time ◽  
Cost Of Care ◽  
Control Group ◽  
P Value ◽  
Control Groups ◽  
And Control ◽  
Wound Healing Time

In the last few decades, the incidence of caesarean section is increasing in the world, especially in Indonesia. One of the way to treat tissue scar is through biologic and synthetic dressing where nowadays, amnion has been used as biologic dressing frequently. This study was conducted to determine the effect of the use of fresh amniotic membrane on wound incision Caesarean section compared with Caesarean section incision wound covered using regular gauze bandages and fixated with plaster in RS. Dr. Reksodiwiryo Padang. The design of this study is an experimen-tal study with Post test design with control group design. Sampling was done using a formula consecutive sampling two different test samples obtained an average of 72 people for each group. The analysis used include univariate and bivariate analyzes. The average wound healing time the difference was statistically significant (p value <0.05) in the treatment and control groups. There was highly significant difference in the proportion of local infection on day 3 between the treatment and control groups (p value <0.05). There were very significant differences in the proportion of local allergic reactions at day 3, and 5 between the treatment and control group (p <0.05). There are significant differences in terms of the cost of care per day between treatment and control groups (p <0.05). From this study, the average wound healing time has a very significant difference.Keywords: Fresh Amniotic Membrane, Wound Cesarean Section, Wound Healing

Evaluation of the Effect of Platelet-Rich Fibrin on Wound Healing at Split-Thickness Skin Graft Donor Sites: A Randomized, Placebo-Controlled, Triple-Blind Study

2020 ◽  
pp. 153473461990043 ◽  
Author(s):  
Mojtaba Vaheb ◽  
Maryam Karrabi ◽  
Mahtab Khajeh ◽  
Alireza Asadi ◽  
Ehsan Shahrestanaki ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Outcome Evaluation ◽  
Control Group ◽  
Platelet Rich Fibrin ◽  
Thickness Skin ◽  
Split Thickness Skin Grafting ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Split-thickness skin grafting (STSG) is widely used to heal wounds resulting from trauma, burns, and chronic wounds. This study aimed to determine the true effect of platelet-rich fibrin (PRF) on patients with burn wounds requiring STSG during treatment of donor wounds. This randomized, triple-blind clinical trial was conducted on patients who referred to the burn ward of Vasei Hospital of Sabzevar, Iran, from May 2017 to May 2018. The donor site was randomly divided into 2 groups: PRF and control (Vaseline petrolatum gauze) using Vaseline gauze. In the intervention group, the PRF gel was applied to the wound and covered with Vaseline gauze and wet dressing. Conversely, only Vaseline gauze and wet dressing were applied to the control group. Outcome evaluation was conducted using paired t test and Wilcoxon signed rank-sum test, as appropriate, on days 8 and 15. The mean age of the patients was 33.10 ± 2.60 years, and 51.50% were male. The mean wound healing time in the PRF and control groups was 11.80 ± 3.51 and 16.30 ± 4.32 days, respectively ( P < .001). The PRF group showed significantly higher wound healing rates than the control group at 8 and 15 days dressing ( P < .001 and P < .001, respectively). Moreover, the mean wound healing for all wound healing indices diagnosed by 2 specialists in PRF was higher than control group on days 8 and 15 ( P < .001). We found a statistically significant difference on days 8 and 15 regarding the mean pain levels between the 2 groups ( P < .001). The findings showed that PRF can significantly increase the time and rate of donor wound healing compared with conventional treatment and also reduce the severity of pain.

scholarly journals CICAFAST: comparison of a biological dressing composed of fetal fibroblasts and keratinocytes on a split-thickness skin graft donor site versus a traditional dressing: a randomized controlled trial

Trials ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Alexandra Poinas ◽  
Pierre Perrot ◽  
Judith Lorant ◽  
Olivier Nerrière ◽  
Jean-Michel Nguyen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Skin Graft ◽  
Donor Site ◽  
Split Thickness Skin Graft ◽  
Skin Grafts ◽  
Thickness Skin ◽  
Fetal Fibroblasts ◽  
Biological Dressing ◽  
Graft Donor

Abstract Background Wound repair is one of the most complex biological processes of human life. Allogeneic cell-based engineered skin substitutes provide off-the-shelf temporary wound coverage and act as biologically active dressings, releasing growth factors, cytokines and extracellular matrix components essential for proper wound healing. However, they are susceptible to immune rejection and this is their major weakness. Thanks to their low immunogenicity and high effectiveness in regeneration, fetal skin cells represent an attractive alternative to the commonly used autologous and allogeneic skin grafts. Methods/design We developed a new dressing comprising a collagen matrix seeded with a specific ratio of active fetal fibroblasts and keratinocytes. These produce a variety of healing growth factors and cytokines which will increase the speed of wound healing and induce an immunotolerant state, with a slight inflammatory reaction and a reduction in pain. The objective of this study is to demonstrate that the use of this biological dressing for wound healing at the split-thickness skin graft (STSG) donor site, reduces the time to healing, decreases other co-morbidities, such as pain, and improves the appearance of the scar. This investigation will be conducted as part of a randomized study comparing our new biological dressing with a conventional treatment in a single patient, thus avoiding the factors that may influence the healing of a graft donor site. Discussion This clinical trial should enable the development of a new strategy for STSG donor-wound healing based on a regenerative dressing. The pain experienced in the first few days of STSG healing is well known due to the exposure of sensory nerve endings. Reducing this pain will also reduce analgesic drug intake and the duration of sick leave. Our biological dressing will meet the essential need of surgeons to “re-crop” from existing donor sites, e.g., for thermal-burn patients. By accelerating healing, improving the appearance of the scar and reducing pain, we hope to improve the conditions of treatment for skin grafts. Trial registration ClinicalTrials.gov, ID: NCT03334656. Registered on 7 November 2017.

scholarly journals Topical Wound Healing Activity of Myricetin Isolated from Tecomaria capensis v. aurea

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4870
Author(s):  
Abdelsamed I. Elshamy ◽  
Naglaa M. Ammar ◽  
Heba A. Hassan ◽  
Walaa A. El-Kashak ◽  
Salim S. Al-Rejaie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Cytokines ◽  
Group Treatment ◽  
Wound Closure ◽  
Burn Injury ◽  
Control Group ◽  
Albino Rats ◽  
Large Area ◽  
Blood Capillaries ◽  
Tnf Α

Wounds and burn injury are major causes of death and disability worldwide. Myricetin is a common bioactive flavonoid isolated naturally from the plant kingdom. Herein, a topical application of naturally isolated myricetin from the shoots of Tecomaria capensis v. aurea on excisional wound healing that was performed in albino rats. The wounded rats were treated every day with 10 and 20% myricetin for 14 days. During the experiment, the wound closure percentage was estimated at days 0, 7, and 14. Effects of myricetin on the inflammatory cytokines (tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and cluster of differentiation 68 (CD68) in the serum were evaluated using immunosorbent assay kits. The percentage of wound closure and contraction was delayed in wounded rats (67.35%) and was remarkably increased after treatment of wounded rats with myricetin; the treatment with 20% myricetin was the most potent (98.76%). Histological findings exhibited that 10% myricetin caused the formation of a large area of scarring at the wound enclosure and stratified squamous epithelium without the formation of papillae as in the control group. Treatment with 20% myricetin exhibited less area of scarring at the wound enclosure as well as re-epithelialization with a high density of fibroblasts and blood capillaries in the wound. Level elevations of serum pro-inflammatory cytokines, IL-1β, and TNF-α and macrophage CD68 were decreased in wounded rats treated with myricetin. Thus, it can be suggested that the enhancements in inflammatory cytokines as well as systemic reorganization after myricetin treatment may be recommended to play a crucial part in the promotion of wound healing. The findings suggest that treatment with a higher dose of myricetin was better in improving wound curing in rats. It could serve as a potent anti-inflammatory agent and can be used as an adjunctive or alternative agent in the future.

Biological versus non-biological dressings in the management of split-thickness skin-graft donor sites: a systematic review and meta-analysis

2020 ◽  
Vol 29 (10) ◽  
pp. 604-610
Author(s):  
Shafiq Rahman ◽  
Benjamin Langridge ◽  
Ahmed Al-Hadad ◽  
Rehman Ali Khan ◽  
Muhammad Hyder Junejo ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Skin Graft ◽  
Meta Analysis ◽  
Healing Time ◽  
Conclusive Evidence ◽  
Split Thickness Skin Graft ◽  
Controlled Trials ◽  
Cochrane Central Register ◽  
Thickness Skin

Objective: There are currently no definitive guidelines regarding the management of split-thickness skin-graft (STSG) donor sites. The literature reports biological and non-biological dressings as the two main groups; however, there is no conclusive evidence regarding the ideal type. A systematic review and meta-analysis of existing clinical trials was performed to compare biological and non-biological dressings in managing STSG donor sites. Method: The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement standards was used to conduct this study. Electronic databases including MEDLINE, Embase, CINAHL and the Cochrane Central Register of Controlled Trials (CENTRAL) were searched by two authors (SR and BL). Data analysis was performed with RevMan 5.3. Results: In total, 10 studies, consisting of eight randomised controlled trials and two observational assessments, were identified. Wound healing time was faster with biological dressings compared to non-biological dressings (mean difference -5.44 days; p<0.05). A higher epithelialisation rate was also noted for biological dressings. There was no difference in the infection rate between the two study groups (odds ratio [OR] 0.39; 95% confidence interval [CI] 0.15–1.04) or wound exudation (OR 0.31; 95% CI 0.01–8.28). The pain level experienced during dressing changes in both groups was reported to be similar. Conclusion: The rate of epithelialisation and wound healing is greater for STSG donor sites when treated with biological dressings, but they offer no difference in terms of reducing pain, limiting infection or exudation.

scholarly journals A Comparison of Wound Healing Rate Following Treatment with Aftamed and Chlorine Dioxide Gels in Streptozotocin-Induced Diabetic Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Fouad Al-Bayaty ◽  
Mahmood Ameen Abdulla
Keyword(s):  
Wound Healing ◽  
Chlorine Dioxide ◽  
Cellular Proliferation ◽  
Radical Scavenging ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Thickness Skin ◽  
Group 2

Background and Purpose. This study aimed to evaluate the wound healing activities of Aftamed and chlorine dioxide gels in streptozotocin-induced diabetic rats. Experimental Approach. Forty-eight Sprague Dawley rats were chosen for this study, divided into 4 groups. Diabetes was induced. Two-centimeter-diameter full-thickness skin excision wounds were created. Animals were topically treated twice daily. Groups 1, the diabetic control group, were treated with 0.2 mL of sterile distilled water. Group 2 served as a reference standard were treated with 0.2 mL of Intrasite gel. Groups 3 and 4 were treated with 0.2 mL of Aftamed and 0.2 mL of chlorine dioxide gels respectively. Granulation tissue was excised on the 10th day and processed for histological and biochemical analysis. The glutathione peroxidase ,superoxide dismutase activities and the malondialdehyde (MDA) levels were determined. Results. Aftamed-treated wounds exhibited significant increases in hydroxyproline, cellular proliferation, the number of blood vessels, and the level of collagen synthesis. Aftamed induced an increase in the free radical-scavenging enzyme activity and significantly reduced the lipid peroxidation levels in the wounds as measured by the reduction in the MDA level. Conclusions. This study showed that Aftamed gel is able to significantly accelerate the process of wound healing in diabetic rats.

scholarly journals Clinical evaluation of the effects of low-intensity laser (GaAlAs) on wound healing after gingivoplasty in humans

2004 ◽  
Vol 12 (2) ◽  
pp. 133-136 ◽  
Author(s):  
Carla Andreotti Damante ◽  
Sebastião Luiz Aguiar Greghi ◽  
Adriana Campos Passanezi Sant'Ana ◽  
Euloir Passanezi
Keyword(s):  
Wound Healing ◽  
Laser Therapy ◽  
Clinical Evaluation ◽  
Test Group ◽  
Sign Test ◽  
Control Group ◽  
Apparent Difference ◽  
Low Intensity ◽  
Low Intensity Laser Therapy ◽  
Intensity Laser

Low-intensity laser therapy aims at pain suppression, edema reduction and acceleration of wound healing. The main goal of this study was to clinically evaluate the effects of Aluminum Gallium Arsenate laser - 670nm in wound healing after gingivoplasty in 11 patients. Surgery was performed in anterior superior and/or inferior regions. The right side of the patient (test group) received a laser energy density of 4J/cm², in a 48-hour interval, during one week, totalizing four sessions. The irradiation was punctual in a contact mode in three points. The left side did not receive irradiation (control group). Clinical evaluation was performed by five specialists in periodontology through photography of the treated areas at post-surgical periods of 7, 15, 21,30,60 days. The observers pointed the best healed side. The Sign test was used for statistical analysis with a confidence level of 5% (P<0.05). The examiners found a better pattern of healing sometimes in the test and sometimes in the control group until 21 days after surgery. After this period there was no apparent difference between them. There was no statistical difference between the sides (p>0.05). These results have shown that low-intensity laser therapy did not accelerate oral mucosa healing after gingivoplasty.

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