scholarly journals Antiproliferative effects of some medicinal plants on HeLa cells

2013 ◽  
Vol 65 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Desanka Cenic-Milosevic ◽  
Z. Tambur ◽  
D. Bokonjic ◽  
S. Ivancajic ◽  
Tatjana Stanojkovic ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Hela Cells ◽  
Melissa Officinalis ◽  
Echinacea Angustifolia ◽  
Antiproliferative Effects ◽  
Hela Cell Lines ◽  
Ic50 Value

Medicinal plants maintain the health and vitality of individuals, and also have potential curative effect on various diseases, including cancer. In this study were investigated the antiproliferative effects of water extracts of previously obtained ethanolic dry extracts of three different medicinal plants (Echinacea angustifolia, Salvia officinalis and Melissa officinalis) on cell lines derived from human cervix adenocarcinoma (HeLa cells). The best cytotoxic activity (IC50 = 43.52 ?g/ml) on HeLa cell lines was exhibited by Echinacea angustifolia. The extract of Salvia officinalis also showed a good cytotoxic activity against HeLa cell lines; the IC50 value was 70.41 ?g/ml. Melissa officinalis manifested a slightly weaker cytotoxic activity and an IC50 value of 122.22 ?g/ml.

scholarly journals Novel C-ring Analogs of Ursolic acid: Synthesis and Cytotoxic Evaluation

2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901
Author(s):  
Uppuluri V. Mallavadhani ◽  
Banita Pattnaik ◽  
Nitasha Suri ◽  
Ajit K. Saxena
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Ursolic Acid ◽  
Parent Compound ◽  
Acid Synthesis ◽  
Hela Cell Line ◽  
Hela Cell Lines ◽  
Protective Groups

Ursolic acid (1), a natural pentacyclic triterpenic acid, afforded a variety of ring-C transformed products (5–11) when treated with N-bromosuccinimide under the influence of a range of protective groups and solvents. The synthesized compounds have been evaluated for cytotoxic activity against prostate PC 3, leukemia THP 1, cervical Hela and lung A-549 cancer cell lines. Among the tested analogs, compounds 5, 8, 9 and 10 showed potent activity against PC 3, THP 1 and Hela cell lines. Especially, compound 10 showed enhanced activity against the Hela cell line than the parent compound. Compounds 5, 8 and 9 showed comparable activities against PC 3 and THP 1 cell lines.

scholarly journals CYTOTOXIC CONSTITUENTS FROM AERIAL PARTS OF HELICTERES HIRSUTA PLANT, COLLECTED IN BINH PHUOC PROVINCE

2018 ◽  
Vol 127 (1A) ◽  
pp. 111
Author(s):  
Pham Huu Dien
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Folk Medicine ◽  
Hep G2 ◽  
Aerial Parts ◽  
Hela Cell Lines ◽  
Human Prostate Carcinoma ◽  
Pure Compounds ◽  
Human Lung Carcinoma

<p><em>Helicteres hirsuta</em> plant is a member of <em>Helicteres </em>genus<em> </em>of the plant family Sterculiaceae and widely found in countries of South Asia such as Vietnam, Lao, Thailand etc. In recent years, it is known as a new folk medicine protecting and securing people against human lung carcinoma, hormone - dependent human prostate carcinoma and human liver. In this paper, from aerial parts of <em>Helicteres hirsuta</em> plant, collected in Binhphuoc province (October, 2016) three pure compounds <strong>1</strong>-<strong>3</strong> were isolated and structurally elucidated, such as <em>b</em>-stigmasterol (<strong>1</strong>), protosta-17(20), 24-dien-3<em>b</em>-ol (<strong>2</strong>) and icosanoic acid (<strong>3</strong>). Among them, compound <strong>2</strong> has a remarkable cytotoxic activity against SK-LU-1, Hep-G2, and Hela cell lines with IC<sub>50</sub> values from 32.86 to 77.31 mg/mL, meanwhile compound <strong>3</strong> shows a moderable cytotoxic activity against SK-Mel-2, AGS, SK-LU-1, Hep-G2, and Hela cell lines with IC<sub>50</sub> values from 59.02 to 80.87 mg/mL.  </p>

scholarly journals Rational Design, Synthesis and Cytotoxic Activity of N-(Phenylcarbamoyl)Benzamide on HeLa Cell Lines

2020 ◽  
Vol 52 (2) ◽  
pp. 174
Author(s):  
Bambang Tri Purwanto ◽  
Siswandono Siswandono ◽  
Suko Hardjono ◽  
Dian Triwidiandany
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Rational Design ◽  
Design Synthesis ◽  
Hela Cell Lines

scholarly journals Synthesis and Antiproliferative Activity of Diethylamine Mannich Base of Asymmetrical Mono-Carbonyl Curcumin Analogs against HeLa Cell Lines

2017 ◽  
Author(s):  
Pekik Wiji Prasetyaningrum ◽  
Anton Bahtiar ◽  
Hayun Hayun
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Mannich Base ◽  
Parent Compound ◽  
Mass Spectral Data ◽  
Mass Spectral ◽  
Curcumin Analogs ◽  
Hela Cell Lines

A series of diethylamine Mannich base of asymmetrical mono-carbonyl analogs of curcumin (AMACs) were synthesized and evaluated for cytotoxic activity against Hela Cell lines. The structures of the synthesized compounds were confirmed on the basis of FTIR, 1H-NMR, 13C-NMR and mass spectral data. Preliminary cytotoxic test using BSLT showed that all the synthesized compounds exhibited more potent cytotoxic activity than that of curcumin. While results of MTT assay showed that all the synthesized compounds exhibited more potent antiproliferative activity against HeLa cell lines than that of cisplatin. Compound 2b exhibited as the most potent compound of the series. Compound 2a, 2b, 2c, and 2f had IC50 (&micro;M) less than that of compound 1a, 1b, 1c and 1f indicating that the addition of diethylamine Mannich base improves the antiproliferative activity of the parent compound.

scholarly journals Dipterostilbenosides A and B, Oligostilbene Glycosides from Dipterocarpus tuberculatus

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900
Author(s):  
Serm Surapinit ◽  
Jonkolnee Jong-aramruang ◽  
Pongpun Siripong ◽  
Suttira Khumkratok ◽  
Santi Tip-pyang
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Spectroscopic Data ◽  
Methanolic Extract ◽  
Chemical Investigation ◽  
Hela Cell Lines

From the chemical investigation of the methanolic extract of the roots of the Thai dipterocarp, Dipterocarpus tuberculatus, two new oligostilbene glycosides, dipterostilbenes A (1) and B (2), were isolated together with four known stilbenes. Their structures and relative configurations were determined on the basis of spectroscopic data. From an evaluation of cytotoxic activity against KB and HeLa cell lines, α-viniferin (5) and (-)-hopeaphenol (6) showed potent activity, but less than that of doxorubicin.

scholarly journals Synthesis and Cytotoxicity Evaluation of Novel Asymmetrical Mono-carbonyl Analogs of Curcumin (AMACs) against Vero, HeLa, and MCF7 Cell Lines

2018 ◽  
Author(s):  
Pekik Wiji Prasetyaningrum ◽  
Anton Bahtiar ◽  
Hayun Hayun
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Mcf7 Cell ◽  
Chemotherapeutic Agents ◽  
Toxic Activity ◽  
Mass Spectral ◽  
Hela Cell Lines ◽  
Ic50 Value ◽  
High Cytotoxic Activity ◽  
Very High

A series of novel asymmetrical mono-carbonyl analogs of curcumin (AMACs) were synthesized and evaluated for cytotoxic activity using the brine shrimp lethality test (BSLT) and the methyl thiazolyl tetrazolium assay against Vero, HeLa, and MCF7 cell lines. The structures of the synthesized compounds were confirmed by Fourier transform infrared spectrophotometry (FTIR), 1H-nuclear magnetic resonance (NMR), 13C-NMR, and mass spectral data. The results of the cytotoxicity evaluation showed that the synthesized compounds exhibited moderate to very high toxic activity in BSLT, requiring a concentration of 13.06&ndash;714.49 &micro;g/mL to kill half the population. Most of the compound exhibited cytotoxic activity against HeLa cell lines, comparable to the activity of cisplatin with a concentration of the synthesized compounds required to inhibit 50% of the growth of the cell lines (IC50) value of 40.65&ndash;95.55 &micro;M, and most of the compounds tested against MCF7 cell lines exhibited moderate to very high cytotoxic activity (IC50 value 7.86&ndash;35.88 &micro;M). However, the selectivity index of the compounds was low, less than 1&ndash;1.96. Among the synthesized compounds, compound 1b showed the highest cytotoxicity and selectivity against MCF7 cell lines. Compound 1b could be considered for further development to obtain more active and selective chemotherapeutic agents against breast cancer.

scholarly journals Isolation and Characterization of Hermetia Illucens Larval Protein for the Assessment of Inhibitory Activity against MCF7 and HeLa cell Lines

2020 ◽  
Vol 9 (3) ◽  
pp. 3045-3050
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Hela Cells ◽  
Inhibitory Activity ◽  
Crude Protein ◽  
The Body ◽  
Assay Method ◽  
Hermetia Illucens ◽  
Isolation And Characterization ◽  
Hela Cell Lines

Cancer has a major impact on society having the highest morbidity and mortality rate. Approximately 70% of deaths are predominantly caused due to cancer and it prevails in low and middle-income countries, as they are unaware of the signs and symptoms, treatment methods, non-availability of cancer drugs and the incurrence of huge economic burden on the family. As per the literature, the chemotherapeutic drug has numerous side effects and occasionally it may lead to death. Based on these inferences, there is a requirement to identify therapeutic peptides that are more effective for cancer treatment with less toxic effects on the body. Peptides have a great advantage to fight against the disease efficiently. Several flies have anticancer, antibacterial, antifungal and antioxidant properties that help to control disease and inhibit cancer progression. In the present research, we have utilized Hermetia illucens has great anticancer peptides that can control cancer growth. In the current research, we utilized crude protein extraction from Hermetia illucens larvae, to discover the inhibitory activity against MCF7 and HeLa cell lines. by adopting the MTT assay method, along with reference drug Camptothecin. Bradford assay results helped to identify that 23.5µg/ml concentration of protein is present in the crude sample. Flow cytometry was used to calculate the % of cell violations and IC50 values were observed in HeLa (19.88±50.3μg/mL) and MCF7 (224.68±50.3μg/mL) cells. Cell cycle inhibition was observed in HeLa cells of G1 (57.3±1.2) and, S (19.8±1.3) phase of dose-dependent with the significance of (P<0.05). Hermetia illucens crude protein has significant inhibition with HeLa cells that have the best anticancer proteins in human cervical carcinoma cells.

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