scholarly journals The antimicrobial activity of honey, bee pollen loads and beeswax from Slovakia

2012 ◽  
Vol 64 (3) ◽  
pp. 927-934 ◽  
Author(s):  
Miroslava Kacániová ◽  
N. Vukovic ◽  
R. Chlebo ◽  
P. Hascík ◽  
Katarína Rovná ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Microscopic Fungi ◽  
Microscopic Fungus ◽  
Bee Pollen ◽  
Pollen Loads ◽  
Different Strains

The aim of this study was to test the antimicrobial activity of propolis, bee pollen loads and beeswax samples collected in the year 2009 from two locations in Slovakia to pathogenic bacteria, microscopic fungi and yeasts. The antimicrobial effect of the bee product samples were tested using the agar well diffusion method. For extraction, 99.9% and 70% methanol (aqueous, v/v) and 96% and 70% ethanol (aqueous, v/v) were used. Five different strains of bacteria, i.e. Listeria monocytogenes ccM 4699, Pseudomonas aeruginosa ccM 1960; Staphylococcus aureus ccM 3953; Salmonella enterica ccM 4420, Escherichia coli ccM 3988, three different strains of microscopic fungi, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, and seven different strains of yeasts Candida krusei, Candida albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis, Geotrichum candidum, Rhodotorula mucilaginosa, were tested. After 48 hours S. aureus was the bacterium most sensitive to the 70% ethanol extract of pollen, A. fumigatus was the most sensitive microscopic fungus (70% ethanol) and C. glabrata the most sensitive yeast (70% methanol). Microorganisms most sensitive to propolis extracts were L. monocytogenes, A. fumigatus (70% ethanol) and G. candidum (70% methanol). Most sensitive to beeswax extracts were E. coli, A. niger and C. tropicalis.

scholarly journals Determination of Antimicrobial Activity of Different Essential Oils Obtained from Plants on Staphylococcus aureus Strains Isolated from Foods

2020 ◽  
Vol 8 (4) ◽  
pp. 1012-1017 ◽  
Author(s):  
Hacer Aslan Canberi ◽  
Esra Şentürk ◽  
Simge Aktop ◽  
Pınar Şanlıbaba
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Thyme Oil ◽  
Staphyloccocus Aureus ◽  
Hypericum Perforatum L ◽  
Different Strains

Essential oils (EOs) are known for its antimicrobial activity against several pathogenic bacteria. The present work evaluated the antimicrobial activity of 15 different EOs on survival of different strains of different Staphyloccocus aureus strains isolated from traditional cheeses by disc diffusion method. The most antimicrobial activity on the strains was found as oil thyme oil (mean zone diameter 23.203 mm). Clove oil and black seed oil had the highest antimicrobial activity after thyme oil with average zone diameters of 13.698 mm and 11.267 mm respectively. Hypericum perforatum L. oil (mean zone 6.209 mm), ginger oil (mean zone 6.250 mm) and garlic oil (mean zone 6.267 mm) were the lowest antimicrobial activity. New studies about antimicrobial effect of EOs in vivo conditions are recommended.

scholarly journals Antimicrobial effect of natural kinds of toothpaste on oral pathogenic bacteria

2021 ◽  
Vol 15 (10) ◽  
pp. 1436-1442
Author(s):  
Serdar Demir ◽  
Gul Keskin ◽  
Nese Akal ◽  
Yasemin Zer
Keyword(s):  
Antimicrobial Activity ◽  
Adverse Effects ◽  
Pathogenic Bacteria ◽  
Natural Kinds ◽  
Aloe Vera ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Oral Pathogens ◽  
Inhibition Zones ◽  
Agar Well Diffusion Method

Introduction: Because of the adverse effects on human health of some antimicrobial ingredients in traditional toothpaste, consumers are increasingly turning to toothpastes with natural ingredients. This study evaluates the antimicrobial effect of toothpastes containing different natural active agents against three oral pathogens: Streptococcus mutans, Streptococcus sanguinis, and Enterococcus faecalis. Methodology: This study tested one traditional toothpaste and seven different natural toothpastes containing theobromine, aloe vera, miswak, propolis, chitosan, enzymes and probiotics. The agar-well diffusion method was used to test the antimicrobial effect. Inhibition zones formed around toothpastes after 24 hours of incubation were measured and the data collected were statistically analyzed. Results: Toothpastes containing theobromine and chitosan and the traditional toothpaste showed antimicrobial efficacy for all tested bacteria. Toothpastes containing aloe vera, miswak, and propolis were only effective on S. mutans, while toothpastes containing probiotics and enzymes did not show any antimicrobial effect on the bacteria. Among toothpastes with natural ingredients, the theobromine-containing toothpaste showed the highest efficacy on S. mutans, while the aloe vera- and propolis-containing toothpastes had the lowest efficacy (p < 0.05). Conclusions: Theobromine- and chitosan-containing toothpastes, which showed antimicrobial activity against all bacteria, can be recommended as alternatives to traditional toothpastes.

scholarly journals Novel Amides Derivative with Antimicrobial Activity of Piper betle var. nigra Leaves from Indonesia

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 335
Author(s):  
Fajar Prasetya ◽  
Supriatno Salam ◽  
Agung Rahmadani ◽  
Kansy Haikal ◽  
Lizma Febrina ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Bacterial Suspension ◽  
Piper Betle ◽  
Yeast Suspension ◽  
Pathogenic Yeast ◽  
Opportunistic Pathogenic ◽  
Promising Source

Piper betle var. nigra is a tropical plant closely related to the common piper. P. betle has also been dubbed a promising source of natural antioxidants in herbal health products, antibacterial, antifungal, antimalarial, cytotoxic activity against the cancer cell lines K562 and HL-60, and antileishmanial. The aim of this study to observation Antimicrobial activity and isolation of chemical compound. The antimicrobial activity of P. betle extract was performed by well diffusion method against two oral pathogenic bacteria (Streptococcus mutans and Streptococcus sanguinis) and opportunistic pathogenic yeast (Candida albicans). The inoculum (bacterial and yeast suspension) was prepared from a 24-h culture on NB for bacterial suspension and on TSB for yeast suspension. Extraction and isolation using various method of chromatography. Isolated compounds were characterized by spectroscopic means. Our study showed antimicrobial activity from crude ethanol extract of leaves P. betle L. var. nigra against two oral pathogenic bacteria and opportunistic pathogenic yeast with concentration 0.5% and 1%. The first report of two new amides derivatives, piperenamide A (1) and piperenamide B (2) in P. betle L. var. nigra.

scholarly journals Phytochemical screening and evaluation of Berginia ligulata root extract for antimicrobial activity

2013 ◽  
Vol 14 (3) ◽  
pp. 101-104
Author(s):  
Rishi Kumar Shukla
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Diethyl Ether ◽  
Pathogenic Bacteria ◽  
Petroleum Ether Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Root Extract ◽  
Phytochemical Screening ◽  
Bacterial Strains

Berginia ligulata popularly known as paashaanbhed belongs to family Saxifragaceae. The present study was designed to screen out various phytoconstituents present in the roots of Berginia ligulata and further its antimicrobial activity against human pathogenic bacteria. For this roots of the plant were shade dried, powdered and extracted using different solvents in increasing order of polarity i.e. petroleum ether, diethyl ether, acetone, ethanol and methanol respectively. Preliminary phytochemical screening of the extracts revealed the presence of steroids, terpenoids, alkaloids, tannins, flavanoids, glycosides and carbohydrates. The presence of these bioactive constituents is associated with the antimicrobial activity of the plant. Antimicrobial activity of extracts was performed by disc diffusion method at a concentration of 100% and 50% (w/v) against five bacterial strains. The maximum antimicrobial activity was found in ethanol extract followed by acetone, methanol, diethyl ether and least in petroleum ether extract respectively. Ethanol extract revealed broad spectrum of inhibition against S. sureus and P. aeruginosa.

scholarly journals Evaluation of the antimicrobial activity of each component in Grossman’s sealer

2006 ◽  
Vol 20 (2) ◽  
pp. 127-131 ◽  
Author(s):  
Ricardo Novak Savioli ◽  
Jesus Djalma Pecora ◽  
Henis Mian ◽  
Izabel Yoko Ito
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Double Layer ◽  
Barium Sulfate ◽  
Antimicrobial Effect ◽  
Sodium Tetraborate ◽  
Diffusion Method ◽  
Similar Activity ◽  
E Coli ◽  
Different Strains

The antimicrobial activity of Grossman’s sealer and its components was evaluated on 13 different strains using the double layer well-diffusion method. Results revealed that Grossman’s sealer presented antimicrobial activity against all the tested strains. Among the components of the cement, sodium tetraborate presented the greatest antimicrobial activity, both in type and diameter of the halo and ring of inhibition. Sealer powder, rosin, and eugenol presented similar activity, with no effect on P. aeruginosa and C. albicans. Among these, only eugenol had an effect on E. coli. Zinc oxide was only active against S. sobrinus and E. coli. Barium sulfate and bismuth subcarbonate did not show any antimicrobial effect.

COMPOSICIÓN QUÍMICA Y ACTIVIDAD ANTIMICROBIANA DE EXTRACTOS DE MACROALGA Gracilaria vermiculophylla SOBRE Vibrio parahaemolyticus/ CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF DIFFERENT EXTRACTS FROM THE MACROALGAE Gracilaria vermiculophylla AGAINST

Biotecnia ◽  
1969 ◽  
Vol 18 (2) ◽  
pp. 27-31
Author(s):  
Pablo Sergio Osuna Amarillas ◽  
Anselmo Miranda Baeza ◽  
Martha Elisa Rivas Vega ◽  
Edgard Esquer Miranda ◽  
Daniel García Bedoya ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Marine Macroalgae ◽  
Diffusion Method ◽  
Gracilaria Vermiculophylla ◽  
Agar Diffusion Method

La búsqueda de compuestos naturales como una alternativa ante el uso de antibióticos sintéticos ha tomado mucha importancia. Se ha demostrado que las macroalgas marinas contienen compuestos capaces de inhibir el crecimiento bacteriano, incluyendo bacterias patógenas para el humano. El objetivo del presente trabajo fue determinar el efecto de dos extractos (acetona y metanol) de la macroalga Gracilaria vermiculophylla sobre el crecimiento de la bacteria Vibrio parahaemolyticus. La determinación del efecto antimicrobiano de los extractos crudos se llevó a cabo utilizando el método de difusión en disco; para lo cual se utilizaron 10, 30 y 50 μL de los extractos en los sensidiscos. Se determinó que el volumen de 50 μL, con ambos extractos presentó los halos de inhibición más altos (3.24 mm para el extracto en metanol y 3.17 mm para el extracto en acetona). ABSTRACT The search for natural compounds as an alternative to the use of synthetic antibiotics has become increasingly important. It has been shown that the marine macroalgae contain compounds capable of inhibiting bacterial growth, including pathogenic bacteria for humans. The aim of this study was to determine the effect of two extracts (acetone and methanol) of macroalgae Gracilaria vermiculophylla on the growth of Vibrio parahaemolyticus. Determination of antimicrobial effect of the crude extracts was performed using the agar diffusion method; for which 10, 30 and 50 μL of the extracts were loaded on sterile antibiotic discs. It was determined that the 50 μL with both extracts showed higher inhibiting zone diameter (3.24 mm for the extract in methanol and 3.17 mm for the extract in acetone).

Bioenhancing Effect of Cow Urine Distillate and Pepper Extract on Antibacterial Activity of Azadirachta Indica Leaves

1970 ◽  
Vol 9 (2) ◽  
pp. 3212-3214
Author(s):  
Pramod Dhakal ◽  
Ankit a Achary ◽  
Vedamurthy Joshi
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Watery Diarrhea ◽  
Diffusion Method ◽  
E Coli ◽  
Drug Molecules ◽  
Cow Urine

Bioenhancers are drug facilitator which do not show the typical drug activity but in combination to enhance the activity of other molecule in several way including increase the bioavailability of drug across the membrane, potentiating the drug molecules by conformational interaction, acting as receptor for drug molecules and making target cell more receptive to drugs and promote and increase the bioactivity or bioavailability or the uptake of drugs in combination therapy. The objective of the present study was to evaluate the antibacterial and activity of combination in Azadirachta indica extract with cow urine distillate and pepper extract against common pathogenic bacteria, a causative agent of watery diarrhea. It has been found that Indian indigenous cow urine and its distillate also possess bioenhancing ability. Bioenhancing role of cow urine distillate (CUD) and pepper extract was investigated on antibacterial activity of ethanol extract of Azadirachta indica. Antibacterial activity of ethanol extract neem alone and in combination with CUD and pepper extract were determined the ATCC strains against Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and E-coli by cup plate diffusion method. Ethanol extract of neem has showed more effect on P. aeruginosa, E-coli than S. aureus and K. pneumonia with combination of CUD and pepper extract. CUD and pepper did not show any inhibition of test bacteria in low concentration. The antibacterial effect of combination of extract and CUD was higher than the inhibition caused by extract alone and is suggestive of the bioenhancing role of cow urine distillate and pepper. Moreover, inhibition of test bacteria was observed with less concentration of extract on combining with CUD

scholarly journals Synthesis of Silver Nano Particles from Adansonia digitata Leaf Extract and Its Antimicrobial Properties

2020 ◽  
pp. 10-22
Author(s):  
A. O. Akintola ◽  
A. M. Azeez ◽  
B. D. Kehinde ◽  
I. C. Oladipo
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Biomedical Application ◽  
Nano Particles ◽  
Diffusion Method ◽  
Adansonia Digitata ◽  
Inhibition Zones

Silver nano particles (AgNPs) were green synthesized using Adansonia digitata leaf extract. The synthesized silver nano particles were characterized in terms of synthesis, size, shape, morphology and capping functionalities by UV-Visible Spectroscopy, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Antimicrobial activity of the synthesized silver nano particles was investigated by well diffusion method. The antibacterial activity of the nano particle was studied against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeurigunosa, Salmonella typhi and Klebsiella pneumonae while the antifungal activity was studied against Candida albicans, Aspergillus niger, Penicillum notatum and Rhizopus stolomifer. The synthesized AgNPs was active against all the studied microorganisms. Staphylococcus aureus was the most susceptible bacterium (inhibition zones ranging from 12.00 to 28.00 mm, MIC: 30 µl, MBC: 50 µl) while Aspergillus niger was the most susceptible fungi (inhibition zones ranging from 10.00 to 18.00 mm, MIC: 90 µl, MFC: 120 µl. In conclusion the synthesized silver nanoparticles was found to have antimicrobial activity against the pathogenic bacteria and fungi tested and hence has a great potential in biomedical application for the treatment of microbial infections.

scholarly journals Antibacterial activity of ginger extracts and its essential oil on some of pathogenic bacteria

2010 ◽  
Vol 7 (3) ◽  
pp. 1159-1165
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Cold Water ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Hot Water ◽  
Diffusion Method ◽  
Resistant Bacteria

The antimicrobial activity of ginger extracts ( cold-water, hot-water, ethanolic and essential oil ) against some of pathogenic bacteria ( Escherichia coli , Salmonella sp , Klebsiella sp , Serratia marcescens, Vibrio cholerae , Staphylococcus aureus , Streptococcus sp) was investigated using Disc diffusion method , and the results were compared with the antimicrobial activity of 12 antibiotics on the same bacteria . The results showed that the ginger extracts were more effective on gram-positive bacteria than gram-negative . V. cholerae and S. marcescens,were the most resistant bacteria to the extracts used , while highest inhibition was noticed against Streptococcus sp (28 mm) . The ethanolic extract showed the broadest antibacterial activity ( 11 to 28 mm ) , in comparison with moderate activity of essential oil , it was observed that the cold-water extract was more effective on the bacteria than hot-water extract . Ginger ethanolic extract presented higher diameter of inhibition zone for Streptococcus sp than in Ciprofloxacin , Cefotaxime , Cefalotin , Cephalexin and Cephaloridine , also it was found a similarity between the higher inhibition zones of ethanolic extract of ginger and some antibiotics for S. aureus , E. coli , Salmonella sp and Klebsiella sp . V. cholerae and S. marcescens,also highly resistant to antibiotics . Phytochemical analysis of ethanolic extract of ginger revealed the present of glycosides, terpenoids, flavonids and phenolic compounds

Comparative studies of the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola on Klebsiella pneumonia and Candida albicans in vitro

2021 ◽  
Vol 16 (1) ◽  
pp. 52-58
Author(s):  
Elizabeth Winful ◽  
Olanikpekun Idowu ◽  
Opeoluwa O. Fasanya ◽  
Nkechi E. Egbe
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Klebsiella Pneumonia ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Garcinia Kola ◽  
Zones Of Inhibition

Antimicrobial resistance has become a global health problem. Although a wide range of chemotherapeutic antimicrobials are available for treatment of microbial related infections and diseases, development of resistance to these chemotherapeutic agents is rapidly on the increase. Extracts from some plants have shown some promise in antimicrobial activity. This has led to the screening of several medicinal plants for their potential antimicrobial activity. Therefore, this study was aimed at evaluating the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola against Klebsiella pneumonia and Candida albicans in vitro. The Garcinia kola seeds extracts were obtained using 70% ethanol and distilled water respectively. Phytochemical screening of Garcinia kola revealed the presence of various potent phytochemicals such as tannins, saponins, flavonoids, alkaloids and glycosides. Both extracts of the seeds were investigated for antimicrobial activity using disc diffusion and agar well diffusion sensitivity tests. The ethanol extract produced zones of inhibition of about 7.3 mm for Candida albicans only at a concentration of 800mg/ml for the disc diffusion test. For the agar well diffusion test, the aqueous extract produced zones of inhibition of about 9.5 mm, while the ethanol extract produced zone of inhibition of 19 mm against Candida albicans at a concentration of 800 mg/ml. However, at the same concentration, the ethanol extract produced zones of inhibition of about 8.5 mm against Klebsiella pneumoniae. The findings of this study revealed that Garcinia kola was not effective in treating Klebsiella pneumonia infections but has potential in treating Candida albicans infections. Keywords: Garcinia kola, Klebsiella pneumonia, Candida albicans

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