scholarly journals Karyotype analysis of some Onobrychis (sainfoin) species in Turkey

2012 ◽  
Vol 64 (2) ◽  
pp. 567-571 ◽  
Author(s):  
Somay Akçelik ◽  
S. Avci ◽  
S. Uzun ◽  
C. Sancak

In this study, karyotype analysis of the species of Onobrychis sp. was undertaken using the squash method. The results showed that the chromosome number of Onobrychis tournefortii (Willd.) Desv., O. gracilis Besser, O. hypargyrea Boiss. is 2n = 14 and O. argyrea Boiss. subsp. argyrea Boiss., 2n = 16. The karyotype formula of O. tournefortii (Willd.) Desv. is 4m+3sm, of O. hypargyrea Boiss. and O. gracilis Besser 3m+4sm, and of O. argyrea Boiss. subsp. argyrea Boiss. 2m+ 5sm+1 st.

2014 ◽  
Vol 62 (3) ◽  
pp. 251 ◽  
Author(s):  
Rocío Deanna ◽  
Gloria E. Barboza ◽  
Marisel A. Scaldaferro

Somatic chromosomes of 12 samples belonging to seven Larnax Miers species and three Deprea Raf. species are studied. Chromosome number and karyotype analysis of both genera are reported for the first time. All taxa have 2n = 24. The most frequent haploid karyotype formula (8 of 12 samples) is 9 metacentric (m) + 3 submetacentric (sm) chromosomes, whereas L. glabra (Standl.) N.W. Sawyer and Larnax sp. display 10 m + 2 sm. Karyotypes of L. nieva S. Leiva & N.W. Sawyer and D. cuyacensis (N.W. Sawyer & S. Leiva) S. Leiva & Lezama are remarkable for the highest number of sm chromosome pairs, with 7 m + 5 sm and 5 m + 7 sm, respectively, presenting the highest intrachromosomal asymmetry index (A1), whereas Larnax sp. and L. glabra show the lowest A1. Most samples (9 of 12) examined have only one pair of chromosomes with nucleolar organiser regions (NOR), whereas L. glabra, Larnax sp., and D. cuyacensis possess two pairs of NOR. Systematic considerations about the monophyly of Larnax and Deprea are provided. The different karyotype parameters obtained, together with morphological characters, are discussed to single out the species.


2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Anupam Guha ◽  
Md. Rabius Sani ◽  
Purabi Banik ◽  
Anita Roy

Houttuynia cordata Thunb. (Saururaceae), has the chromosome number of 2n = 112 with karyotype formula A2+B98+C12. The size of the chromosomal complement was found to range from 1.52 µm to 3.00 µm with one pair of chromosomes bearing secondary constrictions. The detailed karyotype analysis revealed that chromosomes fall under the Stebbins category of 1A, which indicating slightly asymmetric nature of chromosome. The chromosome tally and conformity of the karyotype in the present study corroborated as a new cytotype being adapted in this area, the north-eastern region of India.


2019 ◽  
Vol 32 ◽  
pp. 207-216
Author(s):  
Sadeq S. Kareem Al-Tai ◽  
Sahar A.A. Malik Al-Saadi

Chromosome count, karyotypic character analysis, meiotic studies, monoploid karyograms and ideograms were performed in six taxa of Minuartia growing in Iraq (M. hamata, M. hybrida subsp. hybrida, M. intermedia, M. meyeri, M. picta and  M. hybrida subsp. turcica). Species of M. hamata and M. meyeri showed 2n=2x=30 chromosome number, while M. hybrida subsp. hybrida and M. intermedia  were diploid (26). The chromosome number (n=x) of six species was studied, and was found to be n=15 in M. hamata and M. meyeri, 13 in M. hybrida and M. intermedia, while in M. picta we recorded values of n= 11 and 14. Karyotype analysis of this species was first carried out in our study. Analysis of metaphases showed that the karyotype formula was mainly metacentric, submetacentric, and sub acrocentric. The sizes of the chromosomes were mainly small and very small. The course of meiosis varied from normal to abnormal. Abnormal microsporogenesis formation of two bridge chromosomes was detected in M. hamata and one bridge chromosome in M. intermedia and M. meyeri. Formation of laggard’s chromosomes was detected in M. hamata, M. meyeri   and M. intermedia. As well as ring chromosome was showed in M. hybrida subsp. hybrida, also, some cells contain triad cell in metaphase stage instead four cells, as well as founded cell, contains two nuclei in same species which led to reduced pollen fertility and differences in pollen grain size.


2010 ◽  
Vol 59 (1-6) ◽  
pp. 219-223 ◽  
Author(s):  
Liu Jun ◽  
Ren Bao-Qing ◽  
Luo Peigao ◽  
Ren Zhenglong

Abstract Alnus Mill. (alder) is an ecologically valuable tree genus. It is essential to study its genetic makeup in order to use alder trees to their full potential. Five specimens from four Alnus species (A. mandshurica, A. pendula, A. sibirica, and A. sieboldiana), found in northeastern Asia, were subjected to karyotype analysis. The analysis showed that these tree samples could be divided into three categories based on chromosome numbers or ploidy levels: viz., 2n = (4x) = 28, 2n = (8x) = 56 and 2n = (16x) = 112. The differences in chromosome number and karyotype parameters among Alnus species and even within the same species possibly resulted from natural polyploidization. Comparing the chromosome numbers of Alnus species in China with those in Japan showed that there appear to be only two categories in China, whereas there are up to five categories in Japan. The earliest fossil records of Alnus pollen were also discovered in Japan. We conclude that the center origin of Alnus spp. is Japan rather than China.


1970 ◽  
Vol 40 (1) ◽  
pp. 53-56 ◽  
Author(s):  
Syeda Sharmeen Sultana ◽  
Hosne Ara ◽  
Sheikh Shamimul Alam

Alocasia fallax Schott and A. odora (Roxb.) Koch (Araceae) were investigated cytogenetically to confirm their taxonomic status. There is no report of 2n chromosome number for A. fallax in the available literature and internet information. Therefore the 2n chromosome number (2n = 28) found in this study is probably the first report for A. fallax. Alocasia odora showed exactly double 2n chromosome number (2n = 56) from A. fallax. In addition to chromosome number, the other karyotypic features of A. odora were exactly double for that of A. fallax. The centromeric formulae of A. fallax was 24 m + 4 sm whereas it is just double in A. odora. Total length of 2n chromosome complement of A. odora (62.58 μm) was almost double to A. fallax. The range of chromosomal length of the two species was almost same. Moreover, A. odora plant is much taller than A. fallax. All of these data suggests that A. odora might be an autotetraploid of A. fallax which in course of evolution had undergone some changes in GC-rich repeats. Key words: Alocasia; CMA; Karyotype analysis DOI: http://dx.doi.org/10.3329/bjb.v40i1.7998 Bangladesh J. Bot. 40(1): 53-56, 2011 (June)


2018 ◽  
Vol 6 (2) ◽  
pp. 115-121
Author(s):  
Pushpa Karna Mallick

In this study chromosome number determination and karyotype analysis of four species of genus Blumea from the family asteraceae was carried out. The specimen plants were collected from central parts of Nepal, namely  Blumea fistulosa (Roxb.) Kurz, Blumea lacera var.glandulosa (DC.) Hook, Blumea lacera (Buem f.) DC. and  Blumea laciniata DC were observed. The chromosome number in somatic cells were recorded to be 2n= 22 in Blumea fistulosa; 2n= 32 in  Blumea lacera var.glandulosa; 2n=18 in Blumea lacera and Blumea laciniata. The range of chromosome length found to be 0.6 to 1.6 µm in  Blumea fistulosa, 0.6 to 1.6µm in Blumea lacera var.glandulosa, 0.6 to 1.7 µm in Blumea lacera and 0.8 to 1.6 µm in Blumea laciniata. Karyotype formula for Blumea fistulosa is M12+ sm10, for Blumea lacera var. glandulosa is M14+sm14+ st4, for Blumea lacera is M14+ st2  and for Blumea laciniata is M12+ sm6 in  Blumea laciniata. In this investigation a pair of satellite chromosome found in  only one species Blumea  fistulosa at the end of shot arm of chromosome. Mainly three types of chromosomes observed in this study  having centromere at middle point, at sub-median region and at sub-terminal region. Numerical and structural variation in chromosome are evolutionary significance.  Similarity in size of chromosomes and karyomorphology indicates the homogeneity of the taxa within this tribe. Int. J. Appl. Sci. Biotechnol. Vol 6(2): 115-121


Biologia ◽  
2012 ◽  
Vol 67 (2) ◽  
Author(s):  
Paola Jara-Arancio ◽  
Pedro Jara-Seguel ◽  
Claudio Palma-Rojas ◽  
Gina Arancio ◽  
Raul Moreno

AbstractThe karyotype of fifteen Leucocoryne taxa was studied, assessing characteristics such as chromosome morphology and size, secondary constriction location, and asymmetry level. Two groups of Leucocoryne taxa were described based on chromosome number (2n = 10 and 2n = 18) and karyotype asymmetry. The haploid karyotype formula for the group 2n = 10 was 3m + 2st (or 2t), whereas for the group 2n = 18 was 7m + 2st (or 2t). Such results corroborate the karyotype descriptions previously carried out for some taxa of the genus. Leucocoryne taxa showed a high resemblance in chromosome morphology, but inter-specific differences were found in mean chromosome size. These data and previous studies based on gross chromosome morphology support Crosa’s hypothesis, which suggests that the cytotype 2n = 10 is diploid and perhaps ancestral, whereas that the cytotype 2n = 18 is tetraploid but with an additional chromosome fusion being probably a derived status.


2020 ◽  
Vol 8 (4) ◽  
pp. 422-426
Author(s):  
Pushpa Karna Mallick

In present investigation karyotype of mitotic chromosomes were determined, two species of the genus Spilanthes, namely Spilanthes acmella (L.) Murray and Spilanthes calva DC. from plants transplanted in earthen pots collected from different localities from Central Nepal in my home garden. The somatic chromosome number determined in present study were 2n=36 for both species, Spilanthes acmella (L.) Murray and Spilanthes calva DC. The chromosomes total length ranged from 0.6 to 1.6 µm in Spilanthes acmella (L.) Murray and 0.4 to 2.1µm in Spilanthes calva DC. The relative length ranged from 2.3 to 12.1 µm in the species Spilanthes calva and 2.0 to 8.5 µm in the species Spilanthes acmella. The Karyotype formula were M16+m2+sm16+st2 in Spilanthes calva DC. and M22 +sm14 in Spilanthes acmella (L.) Murray cytologically obtained by using 2% aceto-orcein. The karyotype of the two species shows considerable variation exists in morphology of chromosome and structure. The variation in karyomorphology shows evolved nature of this species which play a great role in evolution. Int. J. Appl. Sci. Biotechnol. Vol 8(4): 422-426


Caryologia ◽  
2021 ◽  
Vol 74 (1) ◽  
pp. 53-61
Author(s):  
Maryam Hasaninejad ◽  
Ziba Jamzad ◽  
Saeid Afsharzadeh ◽  
HojJatollah Saeidi

In this survey, the chromosome counts of eight Nepeta L. species were investigated and the karyotypic diversity among these species was studied. The examined species belong to N. cephalotes Boiss. species group, namely N. eremokosmos Rech.f., N. gloeocephala Rech. f., cephalotes Boiss., N. pungens (Bunge) Benth., N. ispahanica Boiss., N. mahanensis Jamzad & Simonds, N. hormozganica Jamzad and N. denudata Benth. collected from different habitats in Iran. The ploidy levels, karyotype formula, chromosome length range, total karyotype length, several karyotype asymmetries values and Stebbins classification were determined in this study. Results showed the same chromosome number, 2n = 2x= 18 for all studied species. The basic chromosome number for the above mentioned species are x = 9. Also, the smallest chromosome length is 1.02 μm in N. mahanensis. The largest chromosome length is 2.3 μm in N. ispahanica. The chromosomes of species were metacentric or submetacentric. According to the Stebbins classification, these species were located into three classes 1A, 2A and 3A. The chromosome numbers for six of studied species are reported here for the first time.


Author(s):  
Nana Bakhtadze ◽  
Nino Gabroshvili ◽  
Levan Mumladze ◽  
Nino Gabroshvili

Chromosome number data on the Hygromiidae (Gastropoda: Stylommatophora) are summarized and reviewed briefly in the context of the phylogeny of the family. In hygromiids, the haploid chromosome numbers range from 21 to 26. It is supposed that n = 21 is the ancestral chromosome number in the family. The modal haploid number for Hygromiidae is 23. Description of karyotype in terms of chromosome number and morphology of hygromiid land snail Circassina frutis is provided for the first time. The diploid chromosome number of this species is 2n = 46. The karyotype is symmetric and consists of 21 pairs of metacentric and 2 pairs of submetacentric chromosomes. The karyotype formula is as follows: 2n = 42m + 4sm (n = 21m + 2sm). The fundamental number (FN) is 92. Chromosomes range in length from 2.53 μm for the smallest pair to 6.00 μm for the largest pair. The total length of chromosomes in diploid complement (TCL) is 170.40 ± 3.22 μm.


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