scholarly journals Assessment of genetic diversity of Busa cattle from Bosnia and Herzegovina using microsatellite DNA markers

2011 ◽  
Vol 63 (4) ◽  
pp. 1077-1085
Author(s):  
Biljana Rogic ◽  
Lidija Tomic ◽  
B. Vazic ◽  
M. Jelic ◽  
S. Jovanovic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Bosnia And Herzegovina ◽  
Genetic Characterization ◽  
Management Strategies ◽  
Cattle Breed ◽  
Microsatellite Dna Markers ◽  
The Mean ◽  
Two Populations ◽  
Conservation And Management

The variability of 21 microsatellite loci was analyzed in order to estimate the genetic diversity of the Busa cattle breed. A total of 50 animals involved in the study were divided into two groups: Busa from eastern Herzegovina and Busa from western Herzegovina. The mean number of alleles per locus was 6.6. The average expected heterozygosities were 0.6885 and 0.6212 in the eastern and western populations, respectively. The observed heterozygosity values were 0.6579 and 0.6336, in eastern and western population, respectively. The degree of population differentiation (FST) ranged from 0.008 (for ILSTS006 locus) to 0.242 (for BM1818 locus), with a mean of 0.112. Cluster analysis showed that the two populations were clearly clustered into two distinct clades. This report represents the first genetic characterization of pure Busa cattle from Bosnia and Herzegovina. The obtained results are important for the future development of conservation and management strategies for this cattle breed.

scholarly journals Genetic diversity and structure of autochthonous cattle breeds from Bosnia and Herzegovina based on microsatellites

Genetika ◽  
2019 ◽  
Vol 51 (1) ◽  
pp. 335-345
Author(s):  
Biljana Rogic ◽  
Bozo Vazic ◽  
Mihajla Djan ◽  
Marina Stamenkovic-Radak
Keyword(s):  
Genetic Diversity ◽  
Bosnia And Herzegovina ◽  
Genetic Characterization ◽  
Management Strategies ◽  
Cattle Breed ◽  
Total Sample ◽  
Cattle Breeds ◽  
Genetic Diversity And Structure ◽  
Hardy Weinberg Equilibrium

Genotype data from 21 microsatellites were used to evaluate genetic diversity and relationships of Gatacko cattle and Busha breeds. A total of 100 animals included in the study were divided into three groups: Gatacko cattle (GC), Busha from eastern Herzegovina (BEH) and Busha from western Herzegovina (BWH). Hardy-Weinberg equilibrium was observed for all loci in all groups, except for single locus (INRA35) in group of Busha from western Herzegovina. A total number of 166 alleles were detected in a total sample. The average number of alleles per locus was 7.9. The highest observed mean heterozygosity (0.688) was found in the Gatacko cattle and the lowest (0.634) in Busha from western Herzegovina. A pairwise population matrix of Fst values among analyzed groups showed significantly high genetic differentiation among all three groups: Gatacko cattle and Busha from western Herzegovina, Gatacko cattle and Busha from eastern Herzegovina and Busha from western and Busha from eastern Herzegovina. This report represents the first genetic characterization of Gatacko cattle and Busha cattle, traditional cattle breed from Bosnia and Herzegovina. The obtained results are valuable for the future development of conservation and management strategies for these cattle breeds.

scholarly journals Primer Note: Microsatellite-AFLP development for Araucaria araucana (Mol.) K. Koch, an endangered conifer of Chilean and Argentinean native forests

2011 ◽  
Vol 60 (1-6) ◽  
pp. 285-288 ◽  
Author(s):  
G. Marconi ◽  
M. A. Martín ◽  
M. Cherubini ◽  
L. Raggi ◽  
F. Drake ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Native Species ◽  
Conservation Strategy ◽  
Native Forests ◽  
Araucaria Araucana ◽  
The Mean ◽  
Two Populations ◽  
Aflp Technique

Abstract Araucaria araucana (Mol.) K. Koch is one of the most important native species of Chile and Argentina, and also one of the most endangered. In this study, we report the development and characterization of a set of microsatellite markers in the species by means of the microsatellite-AFLP (M-AFLP) technique. A total of 25 M-AFLP derived bands, showing a typical microsatellite pattern, were selected and sequenced. Of these, 12 that contained microsatellite sequences, were used for primer extension. Six of the resulting SSR markers provided easily interpretable patterns and were used to investigate the level of genetic diversity in two populations of A. araucana. A total of 43 alleles were amplified. The mean overall loci of observed and expected heterozygosities for the Conguillio and Villa Araucaria populations were 0.322 and 0.443, respectively. The primers presented in this study may provide useful information for the establishment of a conservation strategy in the species.

scholarly journals Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Zoonotic Potential ◽  
Common Occurrence ◽  
High Genetic Diversity ◽  
Gp60 Gene ◽  
The Common ◽  
Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

Genetic Diversity among Calamus brandisii Becc. (Arecaceae) Populations in Kerala Part of Western Ghats

2021 ◽  
Vol 43 (1) ◽  
pp. 38-42
Author(s):  
Kavungal Priya ◽  
◽  
Indira . ◽  
Vadakkethil Balakrishnan Sreekumar ◽  
Renuka . ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Western Ghats ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Issr Markers ◽  
High Genetic Diversity ◽  
Western Ghats Of India ◽  
The Mean ◽  
Two Parameters ◽  
Two Populations

Calamus brandisii Becc. is one of the endemic slender rattans found in the Western Ghats of India. The genetic diversity of two main populations available in Kerala was investigated using 20 RAPD and 9 ISSR markers. Two parameters viz., gene diversity and genetic diversity within and among populations were analyzed. ISSR analysis showed quite high genetic diversity in Pandimotta compared to Bonacaud population whereas in RAPD markers both these populations were moderately diverse. The percentage of total genetic differentiation (Gst) among two populations is relatively higher than the mean Gst value indicating high genetic diversity within the populations. The genetic distance between these two populations was 0.1739 with ISSR markers and 0.1971 with RAPD markers. Because of its high genetic diversity, Pandimotta population can be treated as an important population of gene diversity with potentially useful genes. This may be included in the high priority reservoir for genetic conservation also.

scholarly journals Random Amplification of Polymorphic DNA Analysis for Genetic Characterization of Two Breeds of Dogs, German Shepherd and Japanese Spitz

2013 ◽  
Vol 13 (2) ◽  
pp. 73-78
Author(s):  
Jarina Joshsi ◽  
Lumanti Manandhar ◽  
Patima Shrestha ◽  
Rani Gupta ◽  
Rojlina Manadhar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Neighbor Joining ◽  
German Shepherd ◽  
Polymorphic Loci ◽  
Random Amplification

Random amplified polymorphic DNA (RAPD) markers were used to study genetic diversity in dog samples belonging to populations of German Shepherd and Japanese Spitz. A total of twelve samples were typed using eight RAPD primers. Out of eight primers, three primers gave result in six individuals of dogs. The phylogenetic tree constructed by the neighbor joining method based on Nei. Original measures revealed highest genetic identity found in German Shepherd as 0.9444 and highest genetic distance as 1.2809. The analysis predicts the number of polymorphic loci as 15 and the percentage of polymorphic loci as 83.3. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 73-78 DOI: http://dx.doi.org/10.3126/njst.v13i2.7717

scholarly journals Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers

2018 ◽  
Vol 22 (1) ◽  
pp. 22
Author(s):  
Jayusman Jayusman ◽  
Muhammad Na’iem ◽  
Sapto Indrioko ◽  
Eko Bhakti Hardiyanto ◽  
ILG Nurcahyaningsih
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Management Strategies ◽  
Genetic Distances ◽  
Sampled Data ◽  
Toona Sinensis ◽  
The Mean ◽  
Individual Trees

Surian Toona sinensis Roem is one of the most widely planted species in Indonesia. This study aimed to estimate the genetic diversity between a number of surian populations in a progeny test using RAPD markers, with the goal of proposing management strategies for a surian breeding program. Ninety-six individual trees from 8 populations of surian were chosen as samples for analysis. Eleven polymorphic primers (OP-B3, OP-B4, OP-B10, OP-H3, OP-Y6, OP-Y7, OP-Y8, OP-Y10, OP-Y11, OP-Y14, and OP-06) producing reproducible bands were analyzed for the 96 trees, with six trees per family sampled. Data were analyzed using GenAlEx 6.3, NTSYS 2.02. The observed percentage of polymorphic loci ranged from 18.2% to 50%. The mean level of genetic diversity among the surian populations was considered to be moderate (He 0.304). Cluster analysis grouped the genotypes into two main clusters, at similarity levels of 0.68 and 0.46. The first two axes of the PCoA explained 46.16% and 25.54% of the total variation, respectively. The grouping of samples into clusters and subclusters did not correspond with family and their distances, but the grouping was in line with the genetic distances of the samples.

scholarly journals Genetic Characterization of Cleveland Bay Horse Breed

Diversity ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 174 ◽  
Author(s):  
Khanshour ◽  
Hempsey ◽  
Juras ◽  
Cothran
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Genetic Similarity ◽  
Genetic Resource ◽  
Genetic Characterization ◽  
Genetic Relationships ◽  
Similarity Measures ◽  
Horse Breed ◽  
The Usa

The Cleveland Bay (CB) is the United Kingdom’s oldest established horse breed. In this study we analyzed the genetic variability in CB horses and investigated its genetic relationships with other horse breeds. We examined the genetic variability among 90 CB horses sampled in the USA compared to a total of 3447 horses from 59 other breeds. Analysis of the genetic diversity and population structure was carried out using 15 microsatellite loci. We found that genetic diversity in CB horses was less than that for the majority of other tested breeds. The genetic similarity measures showed no direct relationship between the CB and Thoroughbred but suggested the Turkman horses (likely in the lineage of ancestors of the Thoroughbred) as a possible ancestor. Our findings reveal the genetic uniqueness of the CB breed and indicate its need to be preserved as a genetic resource.

scholarly journals Genetic, morphological and spatial characterization of two populations of Mabea fistulifera Mart. (Euphorbiaceae), in different successional stages

2005 ◽  
Vol 48 (2) ◽  
pp. 275-284 ◽  
Author(s):  
Maíra Figueiredo Goulart ◽  
Sérvio Pontes Ribeiro ◽  
Maria Bernadete Lovato
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Genetic Data ◽  
Ecological Traits ◽  
Spatial Characterization ◽  
Shannon’S Index ◽  
Successional Stages ◽  
Morphologic Data ◽  
Two Populations

Two populations of Mabea fistulifera (Euphorbiaceae) located at Rio Doce State Park (Minas Gerais, Brazil) in places characterized as in different successional stages, were investigated through genetic and ecological traits. Twenty randomly chosen individuals from each population had its genetic data assessed by 43 RAPD markers and were also evaluated through five morphologic traits and its spatial distribution. Both Shannon's index and AMOVA reveled that most of the genetic variation was found between individuals within populations and about 9% of variation was located between populations. Moreover, populations differed genetically, but presented similar values of genetic diversity. Morphologic data showed differences between populations. Significant correlations among matrix of genetic, geographic and morphologic distances were obtained considering both populations, and spatially closer individuals were more similar genetically, characterizing genetic substructured populations.

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