scholarly journals Optimization of cyclosporin A production by Beauveria nivea in continuous fed-batch fermentation

2011 ◽  
Vol 63 (3) ◽  
pp. 907-914 ◽  
Author(s):  
Huijun Dong ◽  
Junyun Jiang ◽  
Tongshun Yan ◽  
Junjie Zhao
Keyword(s):  
Cyclosporin A ◽  
Batch Fermentation ◽  
Feeding Rate ◽  
Control Method ◽  
Batch Process ◽  
Effective Control ◽  
Fed Batch ◽  
Residual Concentration ◽  
Fed Batch Fermentation ◽  
Continuous Feeding

To develop the effective control method for fed-batch culture of cyclosporin A production, we chose fructose, L-valine and (NH4)2HPO4 as feeding nutrients and compared their productivities in relation to different concentrations. The feeding rate of three kinds of feeding materials was controlled to maintain the suitable residual concentration. The fed-batch fermentation results indicated that the optimal concentrations of fructose, L-valine and (NH4)2HPO4 were about 20 g/L, 0.5 g/L and 0.6 g/L for cyclosporin A production, respectively. The cultivation of Beauveria nivea could produce cyclosporin A up to 6.2 g/L for 240 hrs through a continuous feeding-rate-controlled-batch process under the optimal feeding conditions.

scholarly journals Controlling methanol feeding for recombinant protein production by Pichia pastoris under oxidation stress in fed-batch fermentation

2020 ◽  
Author(s):  
Rongkang Hu ◽  
Dongming Lan ◽  
Ruiguo Cui ◽  
Haomin Hong ◽  
Yachun Niu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pichia Pastoris ◽  
Batch Fermentation ◽  
Protein Production ◽  
Feeding Rate ◽  
Induction Phase ◽  
Fed Batch ◽  
Ros Accumulation ◽  
Fed Batch Fermentation ◽  
Methanol Feeding

Abstract Background: Methanol can be used by Pichia pastoris as the sole carbon source and inducer to produce recombinant proteins in high-cell-density fermentations, but also damages cells due to reactive oxygen species (ROS) accumulation from methanol oxidation. Here, we study the relationship between methanol feeding and ROS accumulation by controlling constant methanol feeding rate during the induction phase.Results: Higher methanol feeding rate increased the level of ROS accumulation caused by methanol oxidation. While the cell growth rate was proportional to the rate of methanol feeding rate, but maximum total protein production and highest enzyme activity were achieved at methanol feeding rate 4 mL/(L·h) as compared to that with 5 mL/(L·h). Moreover, oxidative demage induced by over accumulation of ROS in P. pastoris during the methanol induction phase caused cell death and reduced protein expression ability. ROS scavenging system analysis reveals that the higher methanol feeding rate, especially 5 mL/(L·h), resulted in increased intracellular catalase activity and decreased glutathione content significantly. Finally, Spearman's correlation analysis further reveals that the reduced glutathione might be beneficial for maintaining cell viability and increasing protein production under oxidative stress caused by toxic accumulation of ROS accumulation.Conclusion: Our findings suggest an integrated strategy to control the feeding of the essential substrate based on analyzing its response to oxidative stress caused by toxic accumulation of ROS accumulation, as well as develop strategy to optimize fed-batch fermentation.

scholarly journals Carotenoid Production by Rhodotorula mucilaginosa in Batch and Fed-Batch Fermentation Using Agroindustrial Byproducts

2019 ◽  
Vol 57 (3) ◽  
pp. 388-398 ◽  
Author(s):  
Tábita Veiga Dias Rodrigues ◽  
Thalita D. Amore ◽  
Erika Carvalho Teixeira ◽  
Janaina Fernandes de Medeiros Burkert
Keyword(s):  
Batch Fermentation ◽  
Corn Steep Liquor ◽  
Biomass Concentration ◽  
Batch Process ◽  
Rhodotorula Mucilaginosa ◽  
Fed Batch ◽  
Carotenoid Production ◽  
Fed Batch Fermentation ◽  
Steep Liquor ◽  
Agroindustrial Byproducts

Carotenoids are natural pigments that can be produced through biotechnological processes. However, the costs are relatively high and can be minimized by using lower-cost substrates as alternative nutrient sources. The fed-batch fermentation is one of the techniques used to obtain a high biomass concentration and/or maximum production. Thus, the aim of this work is to produce carotenoids in batch and fed-batch fermentation with the yeast Rhodotorula mucilaginosa CCT 7688 using agroindustrial byproducts in the culture medium. Carotenoid production was increased using experimental designs, which modified the concentration of the agroindustrial medium. In batch production the highest concentrations of total carotenoids (1248.5 μg/L) and biomass (7.9 g/L) were obtained in the medium containing 70 g/L sugar cane molasses and 3.4 g/L corn steep liquor at 25 °C and 180 rpm in 168 h, demonstrating an increase of 17 % when compared to the standard yeast malt medium (1200 μg/L). In the fed-batch production, different feeding strategies were tested with 30 g/L sugar cane molasses and 6.5 g/L corn steep liquor, reaching a total carotenoid production of 3726 μg/L and biomass concentration of 16 g/L. Therefore, the strategy of the fed-batch process resulted in an increase in the carotenoid production of approx. 400 % compared to that in the batch process (740.3 μg/L). Thus, the R. mucilaginosa strain has the potential to produce carotenoids in agroindustrial medium.

scholarly journals Fed-batch fermentation of nipa sap to acetic acid by Moorella thermoacetica (f. Clostridium thermoaceticum)

2017 ◽  
Vol 23 (4) ◽  
pp. 507-514 ◽  
Author(s):  
Dung Nguyen ◽  
Harifara Rabemanolontsoa ◽  
Shiro Saka
Keyword(s):  
Acetic Acid ◽  
Batch Fermentation ◽  
Feeding Rate ◽  
Acetic Acid Concentration ◽  
Fed Batch ◽  
Clostridium Thermoaceticum ◽  
Acid Yield ◽  
Moorella Thermoacetica ◽  
Batch Technique ◽  
Fed Batch Fermentation

An efficient process for conversion of nipa sap to acetic acid was developed. Nipa sap was hydrolyzed with invertase and provided glucose as well as fructose as main sugars. Batch fermentation of glucose and fructose was inadequate with increased substrate concentration. By contrast, fed-batch technique on hydrolyzed nipa sap with high feeding rate drastically increased acetic acid concentration and productivity to be 42.6 g/L and 0.18 g/(L/h), respectively. All the sugars in hydrolyzed nipa sap were consumed, with acetic acid yield of 0.87 g/g sugar. Overall, nipa sap as hydrolyzed with invertase was efficiently fermented to acetic acid, which is a valuable chemical and a potential biorefinery intermediate.

Glycerol Synthesis Attenuation ofCandida glycerinogenesin Fed-batch Fermentation

2012 ◽  
Vol 18 (5) ◽  
pp. 791
Author(s):  
Xiaoyun DING ◽  
Bin ZHUGE ◽  
Huiying FANG ◽  
Hong ZONG ◽  
Xiaoxiao LIU ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Glycerol Synthesis
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