scholarly journals Radical scavenging activity of crude polysaccharides from Camellia sinensis

2011 ◽  
Vol 63 (3) ◽  
pp. 717-721
Author(s):  
Fan Yang ◽  
Zhiwei Yang ◽  
Jianbo Xiao
Keyword(s):  
Molecular Weight ◽  
Free Radical ◽  
Camellia Sinensis ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Concentration Dependent Manner ◽  
Molecular Weights

A preparation of crude polysaccharides (TPS) was isolated from Camellia sinensis by precipitation and ultrafiltration. TPS1, TPS2, and TPS3 had molecular weights of 240, 21.4, and 2.46 kDa, respectively. The radical scavenging activities of TPS were evaluated by DPPH free radical, hydroxyl radical and superoxide radical scavenging. These results revealed that TPS exhibited strong radical scavenging activity in a concentration-dependent manner. TPS3 with lowest molecular weight showed a higher radical scavenging activity.

scholarly journals Phytosterol, Lipid and Phenolic Composition, and Biological Activities of Guava Seed Oil

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2474
Author(s):  
Adchara Prommaban ◽  
Niramon Utama-ang ◽  
Anan Chaikitwattana ◽  
Chairat Uthaipibull ◽  
John B. Porter ◽  
...  
Keyword(s):  
Free Radical ◽  
Serum Lipids ◽  
Seed Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Concentration Dependent Manner ◽  
Guava Seed

Plant seeds have been found to contain bioactive compounds that have potential nutraceutical benefits. Guava seeds (Psidium guajava) are by-products in the beverage and juice industry; however, they can be utilized for a variety of commercial purposes. This study was designed to analyze the phytochemicals of the n-hexane extract of guava seed oil (GSO), to study its free-radical scavenging activity, and to monitor the changes in serum lipids and fatty acid profiles in rats that were fed GSO. The GSO was analyzed for phytochemicals using chromatographic methods. It was also tested for free-radical scavenging activity in hepatoma and neuroblastoma cells, and analyzed in terms of serum lipids and fatty acids. GSO was found to contain phenolic compounds (e.g., chlorogenic acid and its derivatives) and phytosterols (e.g., stimasterol, β-sitosterol and campesterol), and exerted radical-scavenging activity in cell cultures in a concentration-dependent manner. Long-term consumption of GSO did not increase cholesterol and triglyceride levels in rat serum, but it tended to decrease serum fatty acid levels in a concentration-dependent manner. This is the first study to report on the lipid, phytosterol and phenolic compositions, antioxidant activity, and the hepato- and neuro-protection of hydrogen peroxide-induced oxidative stress levels in the GSO extract.

scholarly journals Free Radical Scavenging Capacities and Alleviating Actions of Polysaccharides Extract of Termitomyces le-testui on Methylprednisolone-Induced Immunodepression in Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Foncham Evans Ngwenah ◽  
Kada Sanda Antoine ◽  
Salah Martin ◽  
Tume Christopher ◽  
Oumar Mahamat
Keyword(s):  
Hydrogen Peroxide ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hot Water ◽  
Phagocytic Index ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Concentration Dependent Manner ◽  
Crude Polysaccharide

Background. Natural products have been said to show immunomodulatory and antioxidant activities. The research study was aimed to assess the immunomodulatory and free radical scavenging activities of crude polysaccharide from dry mushroom fruiting bodies of Termitomyces le-testui. Materials and Methods. Hot water extract of polysaccharide extract of T. le-testui was prepared and tested in white albino Wister rats for its immunomodulatory activities effect on methylprednisolone-immunosuppressed animals. In addition, the radical scavenging activity of the polysaccharide was evaluated using nitrite and hydrogen peroxide. Results. The result of the study showed that the polysaccharide T. le-testui increases the phagocytic index, energy metabolism of macrophages, spleen index, and nitric oxide in a concentration-dependent manner in immunosuppressed animals. Also, it was observed that the extract increased dose-dependent total oxidative stress and thymus index. Finally, the crude polysaccharide-rich extract showed nitrite and hydrogen peroxide scavenging activity in a concentration-dependent manner. Conclusion. Polysaccharide-rich extract possesses immunomodulatory and antioxidant properties.

scholarly journals FREE-RADICAL SCAVENGING ACTIVITY LEAF EXTRACT OF LITSEA LAEVIGATA GAMBLE

2019 ◽  
pp. 96-103
Author(s):  
S. SUJATHA ◽  
T. SEKAR
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Metal Chelating

Objective: In the present study, antioxidant activity in the leaf of the pet-ether, chloroform, acetone and methanolic extracts from Litsea laevigata Gamble. Leaf was investigated by employing established in vitro studies. L. laevigata belongs to the Lauraceae family. Methods: The capability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals were determined by in vitro antioxidant assays using 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH.) scavenging, reducing power assay, superoxide radical (O2*-) scavenging activity, phosphomolybdenum assay, FRAP, ABT and metal chelating activity were performed to know the antioxidant potency of the plant extract of leaves of L. laevigata. Results: Results are evaluated higher in leaf extract of L. laevigata recorded total phenol, total flavonoid, and tannin. The present state of work was designed to evaluate the phytochemical, antioxidant in the plant leaf extracts of L. laevigata. The plant L. laevigata methanolic extract of leaf showed greater IC50 antioxidant activity of DPPH assay (5.264 µg/ml) and compare to other extract, higher phosphomolybdenum reduction (164.36 mg/g), better Reducing power activity leaf in methanol (0.711%), higher ferric reducing power (4060.66MmolFe(II)E/mg), and higher in superoxide radical scavenging activity in (78.12 mg/ml). However, the better metal chelating ability was shown by the water extracts of the leaf (5.145 EDTAE/100g) compared to other solvent extracts. Conclusion: The result indicates the total phenol and antioxidant activity potential of L. laevigata.

Antioxidant Activity of Fruits of Perillafrutescens

2014 ◽  
Vol 644-650 ◽  
pp. 5262-5265
Author(s):  
Jing Rong Song ◽  
Gang Lv
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Concentration Dependent Manner ◽  
Dpph Method

The antioxidant activities of extracts and residuum of Perillafrutescens fruits from supercritical CO2 extraction were determined in vitro. The residuum was extracted in turn with water, propyl alcohol and ethyl acetate. The antioxidant activities of the extracts were assayed with antioxidant capacity in linoleic acid model system, reducing powers, radical scavenging activity using 1,1-diphenyl-2-picrylhy-drazyl (DPPH) method. The results show that the ethyl acetate extract of Perillafrutescens possesses strongest DPPH radical scavenging activity and reducing power in a concentration-dependent manner.

Enzymatic Extraction of Polysaccharides from Laminaria Japonica and their Free Radical Scavenging Activity and Antimicrobial Activity Evaluation

2012 ◽  
Vol 479-481 ◽  
pp. 605-610 ◽  
Author(s):  
Jing Chen ◽  
Miao Ping Tian ◽  
Shang Gui Deng ◽  
Xu Bo Fang
Keyword(s):  
Hydroxyl Radicals ◽  
Ph Value ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Laminaria Japonica ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Enzymatic Extraction ◽  
Concentration Dependent Manner ◽  
Potential Applications

Complex enzymes, including cellulase, pectinase and neutral protease were used for extraction of laminaria polysaccharides, which have a broad range of applications in the food, pharmaceutical, agricultural and chemical industries. The enzymatic extraction conditions were optimized and the maximum polysaccharides yield was achieved with the addition of 2.5% of enzymes under a pH value of 5.0 and temperature of 55 °C for 210 min. Polysaccharides prepared under the above conditions were effective against some pathogenic and spoilage microorganisms, including Salmonella sp, Bacillus subtilis, Enterococcus faecalis, Proteus vulgaris, Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens, with a diameter range of 5.1 ± 0.29-12.5 ± 0.78 mm. In addition, antioxidant activity of laminaria polysaccharides against hydroxyl radicals was also investigated and it was observed that the polysaccharides displayed obvious scavenging activity on hydroxyl radicals in concentration-dependent manner. Overall, polysaccharides from Laminaria Japonica demonstrated potential applications in food safety and control.

scholarly journals ANTIMICROBIAL AND FREE RADICAL SCAVENGING ACTIVITY OF LEAF AND STEM EXTRACT OF LIMONIA ALATA WIGHT AND ARN.

2018 ◽  
Vol 11 (4) ◽  
pp. 360
Author(s):  
Malathi S ◽  
Sekar T ◽  
Sujatha S
Keyword(s):  
Free Radical ◽  
Plant Extract ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Metal Chelating

 Objective: In the present study, antioxidant activities leaves and stem of the petroleum, chloroform, acetone, and methanolic extracts from Limonia alata (L.). Leaf and stem were investigated by employing established in vitro studies. The leaves and bark are used for the fomentation of rheumatic pain; the dried fruit is useful in malignant and pestilent fevers and is used as an antidote for poisons, the folklore claim suggests that the leaf is showing wound healing property. L. alata is belonging to the family Rutaceae.Methods: The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, superoxide radical (O2*−) scavenging activity, phosphomolybdenum assay, ferric reducing antioxidant power and metal chelating activity, was performed to know the antioxidant potency and antimicrobial studies of the plant extract of leaves and stem of L. alata.Result: Results are evaluated higher in leaves, stem and root of L. alata (L.) recorded total phenol (59.95±5.30). The present state of work was designed to evaluate the phytochemical, antioxidant as well as to screen the antimicrobial present in the plant extracts of L. alata. The plant L. alata petroleum ether extract of leaf showed greater IC50 for DPPH assay (154.8 μg/mL) and compare to another extract, higher reducing power activity stem in methanol (0.99EDTAE/100 g extract), better phosphomolybdenum reduction (0.512 mg/g extract) higher ferric reducing power (11542.3MmolFe(II)E/mg) extract and higher superoxide radical scavenging activity in leaf extract (332.13%). However, the better metal chelating ability was shown by the water extracts of the leaf (18.24 EDTAE/100 g) compared to other solvent extracts.Conclusion: The result indicates the antioxidant and antibacterial activity potential of L. alata.

Antioxidant Activities of Xiao-Yan-Hua-Jie-San and Identification of the Active Components

2013 ◽  
Vol 781-784 ◽  
pp. 1020-1026
Author(s):  
Yu Ping Li ◽  
Li Hua Yao ◽  
Guang Jie Wu ◽  
Fu Quan Jiang ◽  
Xiang Yuan Xiong ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Model Systems ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Concentration Dependent Manner

Xiao-Yan-Hua-Jie-San (XYHJS), a traditional Chinese prescription, is used as a medication recipe to clinically treat inflammation and hepatitis. In previous study, we reported the hepatoprotective effects of XYHJS by increasing an antioxidant enzyme activity in mice. In the present study, the main bioactive components of XYHJS and their antioxidant activity were further investigated by using different model systems in vitro. The total phenolics content in the extract of XYHJS was determined by the Folin-Ciocalteu method. Analysis of the major phenolic compounds in the extract of XYHYS was carried out by high-performance liquid chromatography (HPLC) and thin layer chromatographic (TLC) method. The total phenolic content of the extract was 2.84 ± 0.06 mg gallic acid equivalent (GAE)/g extract powder. The phenolic acid in XYHJS was found to be gallic acid (GA). The content of GA was 2.80 mg/ml by HPLC (n=5, RSD=1.26%). Furthermore, the antioxidant activity of XYHJS extract was determined by 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity and hydroxyl free radicals (·OH) scavenging activity assay. The radical scavenging activity of XYHJS increased significantly in a concentration-dependent manner. At a concentration of 2.5 mg/ml, the DPPH and ·OH scavenging activity was 89.94% and 91.53%, respectively. It can be concluded that the content of GA in XYHJS is very high and it is the main contributor to the antioxidant activity of XYHJS. Our study indicates that XYHJS prescription could be considered to be an effective agent in the prevention of various liver diseases associated with oxidative stress.

PANCREATIC LIPASE INHIBITORY AND ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF FLORETS OF ASTER SPECIES

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (12) ◽  
pp. 60-68
Author(s):  
R. R Shahu ◽  
◽  
P. Rathod ◽  
C Kulkarni ◽  
S. Mhatre ◽  
...  
Keyword(s):  
Free Radical ◽  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Superoxide Radical ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Pancreatic lipase inhibitory effect of methanolic extract of florets Aster sp. was studied. In case of Aster sp. (White florets) the pancreatic lipase inhibition recorded was 82.75% with an IC50 value of 38μg/mL. Aster sp. (light pink) showed 80.04 % inhibition with an IC50 of 72 μg/mL. On 2 h dialysis, both the extracts lost their significant pancreatic lipase inhibitory activity, indicating the reversible nature of inhibition. pH slightly affected the performance of pancreatic lipase inhibitory activity. Extract (white florets) also showed 22.03% superoxide radical scavenging activity and 22.73% free radical scavenging activity indicating the multifunctional nature of the extract. Light pink florets showed 40.67% superoxide radical scavenging activity and 24.10% free radical scavenging activity. Saponin and alkaloid molecule in white florets of Aster sp. and alkaloid in light pink florets were identified as active principles for pancreatic lipase inhibitory activity.

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