scholarly journals Correlation of the sperm penetration assay (SPA) and miscarriage after assisted reproduction: The potential use of spa as a new criterion for preimplantation genetic diagnosis

2011 ◽  
Vol 63 (1) ◽  
pp. 107-116
Author(s):  
Jelena Gradistanac ◽  
Maria Wikarczuk ◽  
S.G. Somkuti ◽  
L.I. Barmat ◽  
Jay Schinfeld ◽  
...  
Keyword(s):  
Preimplantation Genetic Diagnosis ◽  
Chromosomal Abnormalities ◽  
Sperm Quality ◽  
Genetic Diagnosis ◽  
Fetal Loss ◽  
Vitro Fertilization ◽  
Sperm Penetration ◽  
Sperm Penetration Assay ◽  
New Criterion

We analyzed 93 couples undergoing male screening with the Sperm Penetration Assay (SPA) before in vitro fertilization and intracytoplasmic sperm injection (ICSI), to determine the accuracy of SPA for subsequent embryonic development, incidence of pregnancy and miscarriage rates (SAB). ICSI patients with the lowest SPA scores had significantly higher incidences of Sthan did patients in the other SPA groups. Sperm quality is higher with better SPA scores. Poor sperm quality has increased incidence of chromosomal abnormalities and is associated with early fetal loss. Couples with negative SPA are candidates for preimplantation genetic diagnosis, to reduce the incidence of SAB.

scholarly journals High Aneuploidy Rates Observed in Embryos Derived from Donated Oocytes are Related to Male Aging and High Percentages of Sperm DNA Fragmentation

2015 ◽  
Vol 9 ◽  
pp. CMRH.S32769 ◽  
Author(s):  
Javier García-Ferreyra ◽  
Daniel Luna ◽  
Lucy Villegas ◽  
Rocío Romero ◽  
Patricia Zavala ◽  
...  
Keyword(s):  
Chromosomal Abnormalities ◽  
Sperm Quality ◽  
Genetic Diagnosis ◽  
Paternal Age ◽  
Sperm Dna Fragmentation ◽  
Aging Effects ◽  
Blastocyst Development ◽  
Advanced Paternal Age ◽  
Vitro Fertilization

Capsule Male aging effects on aneuploidy rates in embryos. Objective Paternal age is associated with decreasing sperm quality; however, it is unknown if it influences chromosomal abnormalities in embryos. The objective of this study is to evaluate if the aneuploidy rates in embryos are affected by advanced paternal age. Methods A total of 286 embryos, obtained from 32 in vitro fertilization/intracytoplasmic sperm injection cycles with donated oocytes in conjunction with preimplantation genetic diagnosis, were allocated according to paternal age in three groups: Group A: ≤39 years (n = 44 embryos); Group B: 40-49 years (n = 154 embryos); and Group C: ≥50 years (n = 88 embryos). Fertilization rates, embryo quality at day 3, blastocyst development, and aneuploidy embryo rates were then compared. Results There was no difference in the seminal parameters (volume, concentration, and motility) in the studied groups. Fertilization rate, percentages of zygotes underwent cleavage, and good quality embryos on day 3 were similar between the three evaluated groups. The group of men ≥50 years had significantly more sperm with damaged DNA, low blastocyst development rate, and higher aneuploidy rates in embryos compared to the other two evaluated groups ( P < 0.05). Conclusions Our findings suggest that advanced paternal age increases the aneuploidy rates in embryos from donated oocytes, which suggests that genetic screening is necessary in those egg donor cycles with sperm from patients >50 years old.

Ethical Issues and Practical Problems in Preimplantation Genetic Diagnosis

1998 ◽  
Vol 26 (1) ◽  
pp. 17-28 ◽  
Author(s):  
Jeffrey R. Botkin
Keyword(s):  
Preimplantation Genetic Diagnosis ◽  
Ethical Issues ◽  
Chromosomal Abnormalities ◽  
Genetic Diagnosis ◽  
Embryonic Cells ◽  
Cell Stage ◽  
Vitro Fertilization ◽  
Genetic Abnormalities ◽  
Cellular Dna

Preimplantation genetic diagnosis (PGD) is a new method of prenatal diagnosis that is developing from a union of in vitro fertilization (IVF) technology and molecular biology. Briefly stated, PGD involves the creation of several embryos in vitro from the eggs and sperm of an interested couple. The embryos are permitted to develop to a 6-to-10-cell stage, at which point one of the embryonic cells is removed from each embryo and the cellular DNA is analyzed for chromosomal abnormalities or genetic mutations. An embryo or several embryos found to be free of genetic abnormalities are subsequently transferred to the woman's uterus for gestation. Embryos found to carry a genetic abnormality are discarded or frozen. Extra normal embryos may be frozen for future transfer or donation to another couple.

Problems and prerequisites for determining the legal regime preimplantation genetic diagnosis in Russian legislation

2019 ◽  
Author(s):  
N.A. Altinnik , S.S. Zenin , V.V. Komarova et all ,
Keyword(s):  
In Vitro Fertilization ◽  
Preimplantation Genetic Diagnosis ◽  
Human Embryo ◽  
Legal Status ◽  
Genetic Diagnosis ◽  
Legal Regime ◽  
Vitro Fertilization

Сurrent problems and prerequisites for the formation of the legal regime of pre-implantation genetic diagnosis (PGD) are considered in Russian legislation with account the existing approaches to determining the legal status of a “pre-implantation” embryo obtained in the framework of the in vitro fertilization procedure (IVF) are discussed. The authors substantiates the conclusion that it is necessary to legally determine PGD as one of the stages of using IVF, as well as establishing generally binding requirements for the procedure, conditions and features of this diagnosis, taking into account the need to minimize the damage caused to the human embryo.

Preimplantation genetic diagnosis for the treatment of failed in vitro fertilization–embryo transfer and habitual abortion

2004 ◽  
Vol 81 (5) ◽  
pp. 1302-1307 ◽  
Author(s):  
Martin Wilding ◽  
Robert Forman ◽  
George Hogewind ◽  
Loredana Di Matteo ◽  
Fulvio Zullo ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Transfer ◽  
Preimplantation Genetic Diagnosis ◽  
Genetic Diagnosis ◽  
Vitro Fertilization ◽  
Habitual Abortion
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