scholarly journals Molecular cloning and characterization of glutamate decarboxylase cDNA from the giant-embryo Oryza sativa

2010 ◽  
Vol 62 (4) ◽  
pp. 873-879 ◽  
Author(s):  
Hui Xu ◽  
Pan Liao ◽  
Jianbo Xiao ◽  
Qianfei Zhang ◽  
Yanjun Dong ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Glutamate Decarboxylase ◽  
Structural Features ◽  
Full Length ◽  
Gamma Aminobutyric Acid ◽  
Full Length Cdna ◽  
Gaba Production ◽  
Calculated Molecular Weight ◽  
Giant Embryo

A full-length cDNA encoding glutamate decarboxylase (designated as OsGAD3), which catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), was isolated from the GABA-rich giant-embryo Oryza sativa (Shangshi Jing 315). The full-length cDNA of OsGAD3 (SSJ315) has a 1479 bp open reading frame (ORF) encoding a protein of 492 amino acid residues. The deduced protein had an isoelectric point (pI) of 5.72 and a calculated molecular weight of 56.1 kD. Sequence comparison showed that OsGAD3 (SSJ315) matches the glutamate decarboxylases of other plant species reported previously. Analysis of the structural features of the C-terminal portions of plant GADs revealed that OsGAD3 (SSJ315) has the typical CaM-binding domain (CaMBD) in the C-terminal region as most other plant GADs. Evolution analysis showed that plant GADs are conserved in the process of evolution. The cloning and characterization of the OsGAD3 (SSJ315) gene will enable us to use OsGAD3 to enhance GABA production in O. sativa (SSJ315) by metabolic engineering in the near future.

Characterization of 2159 Unmapped Full-length cDNA Sequences of Oryza sativa L. ssp. japonica ‘Nipponbare’

2009 ◽  
Vol 28 (2) ◽  
pp. 357-362
Author(s):  
Hiroshi Mizuno ◽  
Tsuyoshi Tanaka ◽  
Hiroaki Sakai ◽  
Hiroyuki Kawahigashi ◽  
Takeshi Itoh ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Full Length ◽  
Full Length Cdna ◽  
Cdna Sequences

Cloning and characterization of full-length cDNA clones encoding chalcone synthase from the orchid Bromheadia finlaysoniana

1998 ◽  
Vol 36 (9) ◽  
pp. 647-656 ◽  
Author(s):  
Chye-Fong Liew ◽  
Chong-Jin Goh ◽  
Chiang-Shiong Loh ◽  
Saw-Hoon Lim
Keyword(s):  
Chalcone Synthase ◽  
Full Length ◽  
Cdna Clones ◽  
Full Length Cdna

scholarly journals Characterization of a pathogenic full-length cDNA clone of a virulent porcine epidemic diarrhea virus strain AH2012/12 in China

Virology ◽  
2017 ◽  
Vol 500 ◽  
pp. 50-61 ◽  
Author(s):  
Baochao Fan ◽  
Zhengyu Yu ◽  
Fengjiao Pang ◽  
Xiangwei Xu ◽  
Baimeng Zhang ◽  
...  
Keyword(s):  
Virus Strain ◽  
Cdna Clone ◽  
Porcine Epidemic Diarrhea Virus ◽  
Full Length ◽  
Diarrhea Virus ◽  
Full Length Cdna ◽  
Porcine Epidemic Diarrhea

scholarly journals Rat P45017αfrom Testis: Characterization of a Full-Length cDNA Encoding a Unique Steroid Hydroxylase Capable of Catalyzing Both Δ4- and Δ5-Steroid-17,20-Lyase Reactions

1989 ◽  
Vol 3 (6) ◽  
pp. 968-975 ◽  
Author(s):  
H. Richard Fevold ◽  
Matthew C. Lorence ◽  
John L. McCarthy ◽  
John M. Trant ◽  
Masaaki Kagimoto ◽  
...  
Keyword(s):  
Full Length ◽  
Full Length Cdna ◽  
Steroid Hydroxylase

CHARACTERIZATION OF THE SPLICE SITES IN GT–AG AND GC–AG INTRONS IN HIGHER EUKARYOTES USING FULL-LENGTH cDNAs

2004 ◽  
Vol 02 (02) ◽  
pp. 309-331 ◽  
Author(s):  
SUMIE KITAMURA–ABE ◽  
HITOMI ITOH ◽  
TAKANORI WASHIO ◽  
AKIHIRO TSUTSUMI ◽  
MASARU TOMITA
Keyword(s):  
Alternative Splice ◽  
Splice Site ◽  
Consensus Sequence ◽  
Full Length ◽  
Splice Sites ◽  
Consensus Sequences ◽  
Full Length Cdna ◽  
Strong Consensus ◽  
Higher Eukaryotes

For the purpose of analyzing the relation between the splice sites and the order of introns, we conducted the following analysis for the GT–AG and GC–AG splice site groups. First, the pre-mRNAs of H. sapiens, M. musculus, D. melanogaster, A. thaliana and O. sativa were sampled by mapping the full-length cDNA to the genomes. Next, the consensus sequences at different regions of pre-mRNAs were analyzed in the five species. We also investigated the mononucleotide and dinucleotide frequencies in the extensive regions around the 5' splice sites (5'ss) and 3' splice sites (3'ss). As a result, differential frequencies of nucleotides at the first 5'ss in both the GT–AG and GC–AG splice site groups were observed in A. thaliana and O. sativa pre-mRNAs. The trend, which indicates that GC 5'ss possess strong consensus sequences, was observed not only in mammalian pre-mRNAs but also in the pre-mRNAs of D. melanogaster, A. thaliana and O. sativa. Furthermore, we examined the consensus sequences of the constitutive and alternative splice sites. It was suggested that in the case of the alternative GC–AG introns, the tendency to have a weak consensus sequence at 5'ss is different between H. sapiens and M. musculus pre-mRNAs.

CHARACTERIZATION OF A FULL-LENGTH cDNA FOR RABBIT FACTOR X

1997 ◽  
Vol 85 (6) ◽  
pp. 503-514 ◽  
Author(s):  
Usha R Pendurthi ◽  
Kimberly D Anderson ◽  
Harold L James
Keyword(s):  
Full Length ◽  
Factor X ◽  
Full Length Cdna
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