scholarly journals The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro

2010 ◽  
Vol 62 (1) ◽  
pp. 63-74
Author(s):  
V. Bajic ◽  
Z. Stanimirovic ◽  
Jevrosima Stevanovic ◽  
Zorka Milicevic ◽  
Lada Zivkovic ◽  
...  
Keyword(s):  
Fluorescent In Situ Hybridization ◽  
Human Peripheral Blood ◽  
Chromosome Instability ◽  
Micronucleus Assay ◽  
Peripheral Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Premature Centromere Division ◽  
Combinational Therapies

Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono and/or combinational therapies for cancer treatment.

Cytogenotoxicity of Inclusion Complexes of Diazepam with 2-Hydroxypropyl-β-cyclodextrin

Drug Research ◽  
2017 ◽  
Vol 67 (11) ◽  
pp. 661-672
Author(s):  
Jasmina Hadžiabdić ◽  
Nevenka Kopjar ◽  
Davor Želježić ◽  
Selma Špirtović-Halilović ◽  
Davorka Završnik
Keyword(s):  
Inclusion Complexes ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Genetic Material ◽  
Micronucleus Assay ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Accelerated Proliferation

Abstract Background Diazepam, as one of the most frequent prescribed drug from 1,4-benzodiazepine group, has certain limitations in pharmaceutical technology due to its poor solubility in water. By forming inclusion complexes with 2-hydroxypropyl-β-cyclodextrin, diazepam's biopharmaceutical properties can be greatly improved. Aim Aim of this research was to in vitro evaluate genotoxicity of prepared novel complexes of diazepam and their influence on proliferation of human peripheral blood lymphocytes. Methods For identification of possible genotoxicity of diazepam inclusion complexes, cytokinesis-block micronucleus assay has been chosen. Evaluated concentrations of two diazepam inclusion complexes were 0.2 µg/mL, 0.5 µg/mL and 1.0 µg/mL in cell culture. For a reference, in vitro cytogenotoxicity evaluation of diazepam alone has been conducted as well. Results Neither one of the diazepam, complexed nor non-complexed, in given concentrations showed genotoxicity, induced genetic damage or loss of genetic material. Conclusions Nuclear division index values, as indicators of cytostaticity and cytotoxicity suggested that investigated inclusion diazepam complexes induced accelerated proliferation of human peripheral blood lymphocytes in vitro, therefore possibly shortening the duration and dynamics of the cell cycle.

scholarly journals In vitro cytogenetic toxicity of bezafibrate in human peripheral blood lymphocytes

2017 ◽  
Vol 69 (4) ◽  
pp. 579-589 ◽  
Author(s):  
M. Topaktas ◽  
N. E. Kafkas ◽  
S. Sadighazadi ◽  
E. S. Istifli
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes
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