scholarly journals Characterization of trypsin-like enzymes from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae

2009 ◽  
Vol 61 (4) ◽  
pp. 713-718 ◽  
Author(s):  
N. Loncar ◽  
Natasa Bozic ◽  
Vera Nenadovic ◽  
Jelisaveta Ivanovic ◽  
Z. Vujcic
Keyword(s):  
Anterior Section ◽  
Posterior Section ◽  
Morimus Funereus

The pH along the midgut of M. funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine.

scholarly journals Comparison of strength and depth cut with scalpel on porcine gingival tissues

Duazary ◽  
2020 ◽  
Vol 17 (1) ◽  
pp. 19-26
Author(s):  
Arnulfo Taron-Dunoyer ◽  
Antonio Díaz-Caballero ◽  
Eliana Ávila-Martínez ◽  
Efren Castellar-Vásquez
Keyword(s):  
Shear Force ◽  
Vertical Shear ◽  
Gingival Tissue ◽  
Anterior Section ◽  
Posterior Section ◽  
Significant Difference ◽  
Convenience Sampling ◽  
The Difference ◽  
Cut Depth ◽  
Experimental Trials

The composition of the gums confers some physical characteristics that make it resistant to mechanical stimulation.  The objective of the study was to compare the difference of the utilized forces when performing cuts in the anterior and posterior sections of porcine gingival tissue, measuring the depth of the tissue. A comparative descriptive study was performed with a non-probability convenience sampling, sectioned pig mandibles were used. The experimental trials were performed with an EZ-S SHIMADZU texture analyzer. All of the samples were submitted to a vertical shear force, thus identified the force level used to perform the incision and its depth. the necessary force to perform a cut in porcine gingival tissue was evaluated, comparing the posterior section (39.3571 Newton and 2.160 mm)  and  with the anterior ( 37.8424 newton and 1.747 mm), just as the depth of said cut, showing a statistical difference on the depth, (p=0.022 p< 0.59); regarding the force, no statistically significant difference was found. In the analyzed samples where the shear force in the posterior and anterior section were compared, no difference was found in both groups; as for the cut depth, this was greater in the posterior section than in the anterior.

scholarly journals Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae

2008 ◽  
Vol 60 (3) ◽  
pp. 403-409 ◽  
Author(s):  
Natasa Bozic ◽  
Biljana Dojnov ◽  
Aleksandra Milovanovic ◽  
Vera Nenadovic ◽  
Jelisaveta Ivanovic ◽  
...  
Keyword(s):  
Phenylmethylsulfonyl Fluoride ◽  
Chromogenic Substrates ◽  
Zymogram Analysis ◽  
Morimus Funereus ◽  
Specific Inhibitors

Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities.

Banffia constricta, a putative vetulicolid from the Middle Cambrian Burgess Shale

2005 ◽  
Vol 96 (2) ◽  
pp. 95-111 ◽  
Author(s):  
Jean-Bernard Caron
Keyword(s):  
Deposit Feeder ◽  
Entire Body ◽  
Burgess Shale ◽  
Middle Cambrian ◽  
Anterior Section ◽  
New Class ◽  
Stem Group ◽  
Posterior Section

ABSTRACTAlthough Banffia constricta was first described in 1911, the affinities of this soft-bodied fossil animal have remained unclear. Banffia is redescribed here, on the basis of over 300 specimens. Banffia has a bipartite body measuring up to 10 cm in length. The entire body is axially twisted along a spiral, clockwise as seen from the front. The anterior section consists of two fused carapace-like units with a prominent crown-like structure at the anterior end surrounding the mouth. This crown-like structure is composed of three concentric circlets. A single antenniform outgrowth lies posterior to the mouth. The posterior section of Banffia is composed of 40 to 50 lightly sclerotised segments. The anus is terminal, and situated in a caudal notch. The gut is straight. Simple serially repeated structures along the gut may represent metameric mid-gut diverticulae. Banffia is considered to have been an epibenthic gregarious animal and possibly a deposit-feeder.Banffia is putatively classified as an end-member of the phylum Vetulicolia, in a new class, the Banffozoa. If the vetulicolids represent a stem-group deuterostome, the absence of gills and a possible endostyle in Banffia is problematic. The presence of mid-gut diverticulae in Banffia may represent a link with the protostomes.

A trouser-trawl experiment to assess codends that exclude juvenile mulloway (Argyrosomus hololepidotus) in the Hawkesbury River prawn-trawl fishery

1995 ◽  
Vol 46 (6) ◽  
pp. 953 ◽  
Author(s):  
MK Broadhurst ◽  
SJ Kennelly
Keyword(s):  
Anterior Section ◽  
Trawl Fishery ◽  
Posterior Section ◽  
Simultaneous Comparisons ◽  
By Catch

A trouser trawl was used to assess two codends designed to reduce the by-catch of juvenile mulloway in the Hawkesbury River prawn-trawl fishery. Simultaneous comparisons were made between the catches and by-catches from each codend with those from a conventional codend. The new design incorporated a panel of netting (40-mm mesh or 85-mm mesh) sewn such that the meshes were square-shaped. The panel was placed into the top of the anterior section of the codend to allow water and swimming fish to escape through these larger openings while allowing prawns to tumble along the conventional diamond-shaped netting (40-mm mesh) on the bottom of the codend (and be retained in the posterior section). Comparisons with a conventional codend (in which all meshes were diamond-shaped) showed that the codend with the 40-mm square-mesh panel reduced the by-catch of small mulloway by a mean of 44% without significantly reducing the catch of prawns. The 85-mm square-mesh panel was excluded from analysis, owing to problems associated with its construction.

scholarly journals Patellar Tendon Imbrication

2021 ◽  
Vol 1 (3) ◽  
pp. 263502542110066
Author(s):  
Sercan Yalcin ◽  
Ronak M. Patel ◽  
Jack Andrish ◽  
Lutul D. Farrow
Keyword(s):  
Patellar Tendon ◽  
Patellar Instability ◽  
Patella Alta ◽  
Absorbable Suture ◽  
Skeletally Immature ◽  
Anterior Section ◽  
Posterior Section ◽  
Radiographic Measurements ◽  
Predetermined Level ◽  
Third Line

Background: Patella alta is a common cause of patellar instability. Patellar tendon imbrication is a successful surgery addressing the deformity by shortening the patellar tendon without necessitating an osteotomy. Indications: Symptomatic patella alta causing patellar instability in both skeletally immature and adult patients. Technique Description: The patellar tendon is exposed, and levels of imbrication as determined preoperatively by radiographic measurements are outlined on the tendon with a marking pen. A third line is then made proximal to the level of dissection that is half the distance of the lengthening. From the distal marking, a flap of tendon, the anterior half, is elevated by sharp dissection using a fresh No. 15 blade proximal to the predetermined level. Next, “redundancy-reducing” 2-0 vicryl sutures are placed and left untied by entering at the proximal marking, passing deep to the tendon, and emerging at the middle of the intact posterior section of tendon, followed by placement of 3 structural #2 Ethibond/Ti-Cron horizontal sutures into the patellar tendon that will create and maintain the imbrication. These sutures are then tied proximally while applying downward pressure to the patella to avoid tying under tension. Next, the “redundancy-reducing” sutures are tied thus imbricating the redundant posterior section of tendon. In a “pants over vest” fashion, the distal end of the anterior section of isolated tendon is repaired with ‘‘0’’ absorbable suture. The knee is then flexed beyond 90° to assess competence of the suture lines and to assess the need for quadriceps lengthening. Results: Radiographic shortening is maintained at a minimum of 2 years on 27 patients/32 knees. There were no complications directly related to the technique. Discussion/Conclusion: Patellar tendon imbrication is a safe and effective procedure to correct patella alta in the setting of lateral patellar instability. On average, the technique allowed 1 cm of patellar tendon shortening and maintained the correction at a minimum 2-year follow-up. In the skeletally immature patient, this technique allows correction of patella alta by avoidance of a tibial tuberosity osteotomy.

Quantitative Assessment of the Accuracy of Real-Time Virtual Sonography for Liver Surgery

2019 ◽  
Vol 27 (1) ◽  
pp. 60-67 ◽  
Author(s):  
Akinori Miyata ◽  
Junichi Arita ◽  
Chikara Shirata ◽  
Satoru Abe ◽  
Nobuhisa Akamatsu ◽  
...  
Keyword(s):  
Real Time ◽  
Liver Surgery ◽  
Ct Images ◽  
Actual Measurement ◽  
Anterior Section ◽  
Left Liver ◽  
Computed Tomographic ◽  
Posterior Section ◽  
Adjustment Time ◽  
The Right

Background. Real-time virtual sonography (RVS) is a navigation system for liver surgery. In this study, the degree of misalignment of intraoperative RVS images with computed tomographic (CT) images was measured. Methods. Between December 2014 and July 2015, intraoperative RVS was performed in a total of 33 patients undergoing liver surgery. Reconstructed CT images, rendered like intraoperative ultrasonographic (IOUS) images, were adjusted with the IOUS images and visualized side by side. The degree of misalignment between the reconstructed CT images and IOUS images was measured at anterior section, posterior section, and left liver in each patient. Furthermore, the time required for the adjustment was measured as the “adjustment time.” Results. The degree of misalignment between the images could potentially be measured for a total of 96 points in the 33 patients. Of these, the actual measurement could not be conducted for 35 points due to poor visualization of the intrahepatic vasculature (n = 20) or to a large misalignment that hampered continuation of further adjustment (n = 15). The median degree of misalignment was 9.8 mm (range = 2.4-37.6 mm) in the right anterior section, 9.8 mm (range = 2.7-71.5 mm) in the right posterior section, and 9.5 mm (range = 0.9-37.6 mm) in the left liver. The median adjustment time was 105 seconds (range = 51-245 seconds). Conclusions. Although some misalignment occurred, it might be acceptable for selected situations. Further investigation is needed to reduce the frequency of adjustment failure.

Digestive processes of haematophagous insects. XIII. Evidence for the digestive function of midgut proteinases of Glossina morsitans morsitans Westwood (Diptera: Glossinidae)

1977 ◽  
Vol 55 (9) ◽  
pp. 1557-1562 ◽  
Author(s):  
R. H. Gooding
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Proteolytic Enzymes ◽  
Gel Filtration ◽  
Glossina Morsitans Morsitans ◽  
Glossina Morsitans ◽  
Anterior Section ◽  
Digestive Function ◽  
Posterior Section

Trypsin (EC 3.4.21.4), proteinase VI, proteinase VII, aminopeptidase (EC 3.4.11.1), carboxypeptidase A (EC 3.4.12.2), and carboxypeptidase B (EC 3.4.11.3) occur in the posterior section of the midgut of unfed male and female Glossina morsitans morsitans Westwood, and their activities rise after a blood meal. Only traces of these enzymes occur in the anterior section of the midgut. Elution profiles of proteins during Sephadex gel filtration of the anterior midgut at various times after feeding, and the low ratio of free amino acids to protein in the lumen of the anterior section of the midgut indicate that no significant hydrolysis of protein takes place there. Results of Sephadex® gel filtration of material from the posterior section of the midgut indicate that proteins are rapidly converted to peptides and free amino acids, which occur in high concentration there. The results are interpreted as indicating that digestion of proteins takes place only in the posterior section of the midgut, and that all six proteolytic enzymes have a digestive function.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

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