scholarly journals Characterization of Botrytis cinerea isolates from small fruits and grapevine in Serbia

2009 ◽  
Vol 61 (3) ◽  
pp. 419-429 ◽  
Author(s):  
Brankica Tanovic ◽  
G. Delibasic ◽  
Jasminka Milivojevic ◽  
M. Nikolic
Keyword(s):  
Transposable Elements ◽  
Botrytis Cinerea ◽  
Phenotypic Variability ◽  
Morphological Characterization ◽  
Single Spore

Twenty-six single-spore isolates of Botrytis cinerea from blackberry, raspberry, strawberry, and grapevine were investigated using transposable elements, morphological characterization, and sensitivity to fungicides. Both transposable elements, Flipper and Boty, were detected among isolates from all the hosts. Six vacuma (without transposable elements) and seven transposa (containing both elements) isolates were found to be present in sympatry in Serbia. Isolates containing only the Boty element were detected. Eight morphological types of colonies on PDA and MA media were observed, confirming the great phenotypic variability of B. cinerea. Sensitivity to fungicides was various, depending on both the fungicide and the isolate.

scholarly journals Detection and Characterization of Botrytis cinerea Isolates from Vegetables in Egypt

2019 ◽  
Vol 8 (3) ◽  
pp. 77-85
Author(s):  
Mahmoud A Gaber ◽  
Elsayed E Wagih ◽  
Mohamed R. A Shehata ◽  
Magda M Fahmy ◽  
Hala Abdel Wahab
Keyword(s):  
Transposable Elements ◽  
Botrytis Cinerea ◽  
Plant Pathogen ◽  
Molecular Characteristics ◽  
Molecular Variation ◽  
Phenotypic Characteristic ◽  
Phenotypic Characteristics ◽  
Crop Losses

Botrytis cinerea is a necrotrophic plant pathogen that causes plenty of crop losses in Egypt and worldwide. Fifteen isolates of B. cinerea were collected from cabbage, pepper and lettuce grown in different locations in Egypt and subjected to investigation. Diversity in phenotypic, pathological and molecular characteristics was detected among isolates, leading to categorising them into four different groups. Molecular variation was demonstrated in all isolates by transposable elements (TEs) analyses. Four TE types, based on the presence or absence of two transposable elements, boty and flipper, were recognised among B. cinerea isolates in which transposa type (having both TE, boty + flipper) was predominant (40%), while only boty and only flipper types appeared with distribution values of 26.7 and 20%, respectively and vacuma type (Lacking both TEs) showed the lowest distribution value (13.3%). Furthermore, vacuma population demonstrated the lowest potential comparing to others. A correlation was found between TE type and virulence level of isolate, but no impact of TE type was observed on phenotypic characteristics of B. cinerea. The present study revealed a correlation between the TE type and the isolate virulence, but no correlation was observed between phenotypic characteristic/sensitivity to fenhexamid and TE type

scholarly journals Phenotypic and genetic characterization of Botrytis cinerea isolates from tomato

Genetika ◽  
2012 ◽  
Vol 44 (3) ◽  
pp. 633-647 ◽  
Author(s):  
Biljana Kuzmanovska ◽  
Rade Rusevski ◽  
Ljupcho Jankuloski ◽  
Mirjana Jankulovska ◽  
Dario Ivic ◽  
...  
Keyword(s):  
Transposable Elements ◽  
Botrytis Cinerea ◽  
Genetic Characterization ◽  
Morphological Type ◽  
Republic Of Macedonia ◽  
High Tunnels ◽  
The Republic ◽  
Phenotypic And Genetic Characterization

One hundred and twenty-three isolates of Botrytis cinerea were collected from 7 different areas in the Republic of Macedonia, where tomato is mostly grown in greenhouses and high tunnels. Based on the mycelial formation, intensity of sporulation and sclerotial production, 9 different phenotypes were detected: 4 mycelial and 5 sclerotial. One sclerotial morphological type has not been previously reported. The presence or absence of two transposable elements, boty and flipper, was detected by PCR. Out of 123 isolates, 20 had two transposable elements, boty and flipper (transposa genotype), 48 had neither of these elements (vacuma genotype) and 55 had only the flipper element (flipper genotype). Isolates that contain only boty element were not detected. No relationship between the phenotypes, origin of isolates and the presence/absence of transposable elements, boty and flipper, was found.

scholarly journals Morphological characterization of active germoplasm bank fig tree accessions

2019 ◽  
Vol 41 (5) ◽  
Author(s):  
Maria Gabriela Fontanetti Rodrigues ◽  
Tatiane Paes dos Santos ◽  
Antonio Flávio Arruda Ferreira ◽  
Laís Naiara Honorato Monteiro ◽  
Eduarda Sayuri Nakanishi ◽  
...  
Keyword(s):  
Crop Production ◽  
Phenotypic Variability ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Qualitative And Quantitative ◽  
Fig Tree ◽  
Fig Trees ◽  
Selection Of ◽  
Qualitative And Quantitative Characteristics

Abstract Fig tree (Ficus carica L.) is a fruit of great importance worldwide, being able to be implanted as new source of agricultural variability. The aim of the present work was to characterize the active germplasm bank of fig trees by means of morphological descriptors of 45 fig accessions at the Faculty of Agrarian and Technological Sciences, Campus of Dracena, FCAT / UNESP aiming at the recognition of the quality and genetic variability potential of the species. Thus, their biometric characteristics were evaluated by qualitative and quantitative descriptors of leaves and fruits. Fig tree accessions were characterized by means of the morphological characteristics of leaves and fruits, identifying AGB with high phenotypic variability and well adapted to the region and, by the analysis of the qualitative and quantitative characteristics, it was concluded that the most divergent accessions presented characteristics of agronomic interest, allowing the selection of features in order to subsidize conservation works, genetic improvement and crop production.

scholarly journals Genetic variability in clones of 'Prata Anã' bananas based on phenotypic and molecular markers

Bragantia ◽  
2012 ◽  
Vol 71 (2) ◽  
pp. 182-189 ◽  
Author(s):  
Francisco Ermelindo Rodrigues ◽  
Samira Santiago Librelon ◽  
Silvia Nietsche ◽  
Márcia Regina Costa ◽  
Marlon Cristian Toledo Pereira
Keyword(s):  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Phenotypic Variability ◽  
Qualitative Evaluation ◽  
Morphological Characterization ◽  
Banana Tree ◽  
Minas Gerais State ◽  
Polymorphic Bands ◽  
Dna Primers

The objective of this study was to examine the molecular and phenotypic variability of 'Prata Anã' banana clones cultivated in northern Minas Gerais State. For the phenotypic and molecular characterization, the clones were collected from five properties. The morphological characterization of 20 clones was accomplished through a qualitative evaluation of 74 descriptors. For the molecular evaluations, 45 clones were collected, and 14 Random Amplified Polymorphic DNA primers were used. Among the 74 morphological descriptors evaluated in the 20 'Prata Anã' banana tree clones, 53 descriptors presented in the same homogeneous class for all clones. The presence of anthocyanin in the pseudostem (ANT) and the shape (BUS) and flexion (FLX) of the bunch clearly differentiated the 'Prata Anã' banana clones from the commercial cultivar. A total of 176 bands were produced from the molecular analysis using the 14 RAPD primers; there were 116 monomorphic and 60 polymorphic bands, with an average polymorphism percentage of 52.2%. The amplification using the OPP 14 primer resulted in the greatest percentage of polymorphic bands at 88%. The dendrogram indicated that each of the 45 'Prata Anã' clones that were evaluated using RAPD markers clustered with the commercial 'Prata Anã' cultivar.

Single spore isolation and morphological characterization of local Malaysian isolates of rice blast fungus Magnoporthe grisea

2015 ◽  
Author(s):  
Ankitta Mishra ◽  
Wickneswari Ratnam ◽  
Md Atiqur Rahman Bhuiyan ◽  
Ariane Ponaya ◽  
Khisord K. Jena
Keyword(s):  
Rice Blast ◽  
Rice Blast Fungus ◽  
Morphological Characterization ◽  
Single Spore ◽  
Blast Fungus

scholarly journals Agro-morphological characterization of Bambara nut accessions [Vigna subterranea (L) Verdcourt] from Burkina Faso

2020 ◽  
Vol 153 ◽  
pp. 15727-15744
Keyword(s):  
Burkina Faso ◽  
Phenotypic Variability ◽  
Morphological Diversity ◽  
Morphological Characters ◽  
Morphological Characterization ◽  
Vigna Subterranea ◽  
Breeding Programs ◽  
Ecological Zones ◽  
Total Variability

Objectives: The agro morphological diversity of Bambara nut remains poorly understood. In this study 90 Bambara nut accessions were assessed for phenotypic variability of agronomic and morphological traits. Methodology and results: A trial was conducted at Rollo (Bam province) on 90 accessions of Bambara nut of 3 agro ecological zones in Burkina Faso. Accessions were evaluated on 27 morphological characters. Result of qualitative traits analysis showed dominance of cream-coloured seeds, butterfly-grey eyes, and smooth yellowish-brown pods. The majority of quantitative traits were discriminant at 1%. The completed PCA indicates that the first two axes expressed 55.60% of the total variability. The dendogram highlighted four groups of diversity. Conclusion and application of results: The agro morphological characterization showed great variability within the accessions studied. The different associations between yield components and the clustering into classes could be exploited in breeding programs for improvement of Bambara nut productivity. RESUME Objectifs: La diversité agro morphologique du pois bambara reste mal connue. Dans cette étude, la variabilité phénotypique des caractères agronomiques et morphologiques de 90 accessions de pois bambara a été évaluées. Méthodologie et résultats: Un essai a été conduit à Rollo (Province de Bam) sur 90 accessions de pois bambara issues de quatre zones agro-écologiques du Burkina Faso. Les accessions ont été évaluées sur 27 caractères morphologiques. Les résultats de l’analyse des caractères qualitatifs ont montré la dominance des graines de couleur crème, des yeux gris papillon et des gousses lisses brun jaunâtre. La majorité des Caractères quantitatifs étaient discriminants à 1%. L'ACP indique que les deux premiers axes ont exprimé 55, 60% de la variabilité totale. Le dendrogramme a mis en évidence quatre groupes de diversité. Kambou et al., J. Appl. Biosci. 2020 Agro-morphological characterization of Bambara nut accessions [Vigna subterranea (L) Verdcourt] from Burkina Faso 15728 Conclusion et application des résultats: La caractérisation agro morphologique a montré une grande variabilité au sein des accessions étudiées. Les différentes associations entre les composantes du rendement et la subdivision en classes pourraient être exploités dans les programmes de sélection pour l'amélioration de la productivité du pois bambara. Mots clés: Pois bambara, caractères morphologiques, variabilité

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

Morphological Characterization of Rhinacanthus Species of Sri Lanka

Planta Medica ◽  
2010 ◽  
Vol 76 (05) ◽  
Author(s):  
APPR Amarasinghe ◽  
RP Karunagoda ◽  
DSA Wijesundara
Keyword(s):  
Sri Lanka ◽  
Morphological Characterization

Density and morphological characterization of bacteria in integrated and exclusive production systems

2020 ◽  
Vol 13 (11) ◽  
pp. 1
Author(s):  
A. R. B. Zanco ◽  
A. Ferreira ◽  
G. C. M. Berber ◽  
E. N. Gonzaga ◽  
D. C. C. Sabino
Keyword(s):  
Bacterial Community ◽  
Rainy Season ◽  
Production Systems ◽  
Cropping System ◽  
Morphological Characterization ◽  
Native Forest ◽  
Exclusive Production ◽  
Colony Forming Units

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season

Characterization of Antibacterial-Producing Endophytic Fungi of Syzygiumpolyanthum Leaves

2020 ◽  
Vol 20 (4) ◽  
pp. 448-454
Author(s):  
Rahmita Burhamzah ◽  
Gemini Alam ◽  
Herlina Rante
Keyword(s):  
Molecular Characterization ◽  
Endophytic Fungi ◽  
Morphological Characterization ◽  
Test Method ◽  
Analysis Method ◽  
Nitrogen Base ◽  
Rdna Genes ◽  
Antibacterial Screening ◽  
Planting Method

Background: Endophytic fungi live in plants’ tissue and can produce the same bioactive compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of the antibacterial compound producers. Aim and Objective: This study aimed to characterize morphologically, microscopically, and molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves. Methods: The isolation of endophytic fungi was done by fragment planting method on PDA medium. The antibacterial screening was performed using the antagonistic test as the first screening followed by the disc diffusion test method. The morphological characterization was based on isolate’s mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic characterization was based on its hyphae characteristics. The molecular characterization of the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS rDNA genes of the isolate. Results: The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus, P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation extract on S.aureus with a diameter of inhibition of 13.77 mm. Conclusion: Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is similar to Ceriporialacerate.

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