scholarly journals Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench)

2007 ◽  
Vol 59 (2) ◽  
pp. 119-124
Author(s):  
Mira Milisavljevic ◽  
Gordana Timotijevic ◽  
Svetlana Radovic ◽  
M.M. Konstantinovic ◽  
Vesna Maksimovic
Keyword(s):  
Structural Analysis ◽  
Small Group ◽  
Cdna Library ◽  
Plant Species ◽  
Bioinformatics Analysis ◽  
Aspartic Proteinase ◽  
Genomic Fragment ◽  
Gene Coding ◽  
New Perspective ◽  
Fagopyrum Esculentum Moench

A novel type of aspartic proteinase gene was isolated from the cDNA library of developing buckwheat seeds. This cDNA, FeAPL1, encoded an AP-like protein lacking the plant-specific insert (PSI) domain characteristic of typical plant aspartic proteinases. In addition the corresponding genomic fragment was isolated. It is demonstrated that this gene does not contain introns. Since bioinformatics analysis of the Arabidopsis genome showed that most potential AP genes are intronless and PSI-less, it appears that "atypical" is an inappropriate word for that class of AP. Isolation of this specific buckwheat gene among the small group of those isolated from other plant species provides a new perspective on the diversity of AP family members in plants. .

scholarly journals Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench)

2010 ◽  
Vol 62 (1) ◽  
pp. 143-151 ◽  
Author(s):  
Gordana Timotijevic ◽  
Mira Milisavljevic ◽  
Svetlana Radovic ◽  
M.M. Konstantinovic ◽  
Vesna Maksimovic
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cdna Library ◽  
Seed Development ◽  
Potential Role ◽  
Aspartic Proteinase ◽  
Fagopyrum Esculentum ◽  
High Homology ◽  
Aspartic Proteinases ◽  
Fagopyrum Esculentum Moench

Aspartic proteinase gene (FeAP12) has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP) characterized by the presence of a plant-specific insert (PSI), unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.

scholarly journals Transcriptome-Based Identification of a Functional Fasciola hepatica Carboxylesterase B

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1454
Author(s):  
Yaretzi J. Pedroza-Gómez ◽  
Raquel Cossio-Bayugar ◽  
Hugo Aguilar-Díaz ◽  
Silvana Scarcella ◽  
Enrique Reynaud ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Dna Sequences ◽  
Bioinformatics Analysis ◽  
Fasciola Hepatica ◽  
Polyacrylamide Gel Electrophoresis ◽  
Cellular Membrane ◽  
Reading Frame ◽  
Protein Mass ◽  
Gene Coding ◽  
Sds Page

Bioinformatics analysis of the complete transcriptome of Fasciola hepatica, identified a total of ten putative carboxylesterase transcripts, including a 3146 bp mRNA transcript coding a 2205 bp open reading frame that translates into a protein of 735 amino acids, resulting in a predicted protein mass of 83.5 kDa and a putative carboxylesterase B enzyme. The gene coding for this enzyme was found in two reported F. hepatica complete genomes stretching 23,230 bp, containing two exons of 1282 and 1864 bp, respectively, as well as a 20,084 bp intron between the exons. The enzymatic activity was experimentally assayed on F. hepatica protein extracts by SDS-PAGE zymograms using synthetic chromogenic substrates, confirming both the theoretical molecular weight and carboxylesterase enzymatic activity. Further bioinformatics predicted that this enzyme is an integral component of the cellular membrane that should be active as a 167 kDa homodimer complex and polyacrylamide gel electrophoresis (PAGE) zymograms experiments confirmed the analysis. Additional bioinformatics analysis showed that DNA sequences that code for this particular enzyme are highly conserved in other parasitic trematodes, although they are labeled hypothetical proteins.

Genetic and morphological analysis of floral homeotic mutants tepal-like bract and fagopyrum apetala of Fagopyrum esculentum

Botany ◽  
2008 ◽  
Vol 86 (4) ◽  
pp. 367-375 ◽  
Author(s):  
Maria D. Logacheva ◽  
Ivan N. Fesenko ◽  
Aleksey N. Fesenko ◽  
Aleksey A. Penin
Keyword(s):  
Plant Species ◽  
Morphological Analysis ◽  
Morphological Evolution ◽  
Single Gene ◽  
Gene Mutations ◽  
Fagopyrum Esculentum ◽  
Wide Range ◽  
Fagopyrum Esculentum Moench ◽  
Mutant Phenotypes ◽  
Floral Homeotic

The studies on floral homeotic mutants of the model plant species Arabidopsis thaliana (L.) Heynh. and Antirrhinum majus L. have clarified many important aspects of the genetic control of flower development. However, the details of this process can vary in species representing different lineages of flowering plants. The studies on floral homeotic mutants of nonmodel plant species may significantly improve the understanding of the mechanisms of morphological evolution of flowers. We report here the results of the genetic and morphological analysis of two floral homeotic mutants of common buckwheat ( Fagopyrum esculentum Moench.). The mutant, tepal-like bract (tlb), is characterized by the transformation of bracts into petaloid organs, whereas fagopyrum apetala (fap), has a carpelloid perianth. Both mutant phenotypes are caused by a single recessive nuclear mutation. The double mutant fap tlb combines the features of tlb and fap. Our results show that single gene mutations are sufficient to convert the buckwheat bract into a tepal and to confer carpel identity on first whorl organs. These results are consistent with the premise that variations on the ABC model can be used to explain a wide range of floral architectures.

Assessment of the suitability of Plagiohammus spinipennis (Thoms.) (Col., Cerambycidae) as an agent for control of weeds of the genus Lantana (Verbenaceae). II. Host specificity

1969 ◽  
Vol 58 (4) ◽  
pp. 787-792 ◽  
Author(s):  
K. L. S. Harley ◽  
R. K. Kunimoto
Keyword(s):  
Small Group ◽  
Host Plant ◽  
Host Specificity ◽  
Plant Species ◽  
Deleterious Effect ◽  
Tectona Grandis ◽  
Normal Host ◽  
Commercial Importance ◽  
High Degree ◽  
Test Plants

Results are presented of a study of the host specificity of Plagiohammus spinipennis (Thorns.) under conditions that emphasised the behaviour of the insect in the field in the presence of an abundance of its host-plant, Lantana cantata. The results of this and previous studies are compared.The adult beetles fed on various test plants but had little or no deleterious effect. Oviposition was restricted to the insect’s normal host, L. camara, and to Cordia ambigua, G. grandiflora, G. curassavica, Lippia umbellata, Lippia sp. (? miocephala), Tectona grandis and Vernonia deppeana. Of these, only T. grandis is of commercial importance. Larvae were unable to complete their development on this plant and died after feeding briefly. Development by larvae of P. spinipennis was restricted to a small group of mostly related, unimportant plant species as well as the serious weed L. camara.P. spinipennis thus shows a high degree of host specificity, and was therefore liberated for control of Lantana camara in Australia in 1967.

Dictyostelium discoideum contains two profilin isoforms that differ in structure and function

1991 ◽  
Vol 100 (3) ◽  
pp. 481-489 ◽  
Author(s):  
M. Haugwitz ◽  
A.A. Noegel ◽  
D. Rieger ◽  
F. Lottspeich ◽  
M. Schleicher
Keyword(s):  
Amino Acid ◽  
Cdna Library ◽  
Dictyostelium Discoideum ◽  
Actin Polymerization ◽  
Critical Concentration ◽  
Exchange Chromatography ◽  
Amino Acid Sequences ◽  
Actin Binding ◽  
Gene Coding ◽  
And Function

Two profilin isoforms (profilins I and II) have been purified from Dictyostelium discoideum, using affinity chromatography on a poly(L-proline) matrix; the isoforms could be separated by cation-exchange chromatography on a FPLC system. The gene coding for profilin I was cloned from a lambda gt11 cDNA library using a profilin I-specific monoclonal antibody. The profilin II cDNA was isolated by probing the cDNA library with an oligonucleotide deduced from the N-terminal amino acid sequence of profilin II, which has an open N terminus in contrast to profilin I. The deduced amino acid sequences of both genes show that profilin I in comparison to profilin II is slightly larger (13,064 Da vs 12,729 Da), has a more acidic isoelectric point (calc. pI 6.62 vs 7.26) and shares with profilin II 68 identical residues out of 126 amino acids. Although both profilins contain a conserved lysine residue in the putative actin-binding region and can be crosslinked covalently to G-actin, the crosslinking efficiency of profilin II to actin is substantially higher than that of profilin I. These data are in agreement with studies on the functional properties of the profilin isoforms. In most preparations profilin II was more efficient in delaying the onset of elongation during the course of actin polymerization and caused a higher critical concentration for actin polymerization than profilin I, probably due to the slightly increased affinity of profilin II for D. discoideum G-actin (approx. Kd 1.8 × 10(−6) M) as compared to that of profilin I (approx. Kd 5.1 × 10(−6) M).(ABSTRACT TRUNCATED AT 250 WORDS)

Structural analysis of a maize gene coding for glutathione-S-transferase involved in herbicide detoxification

1986 ◽  
Vol 6 (4) ◽  
pp. 203-211 ◽  
Author(s):  
Dilip M. Shah ◽  
Cathy M. Hironaka ◽  
Roger C. Wiegand ◽  
Elizabeth I. Harding ◽  
Gwen G. Krivi ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Glutathione S Transferase ◽  
Gene Coding ◽  
Herbicide Detoxification ◽  
Maize Gene

scholarly journals Economic strategies of plant absorptive roots vary with root diameter

2016 ◽  
Vol 13 (2) ◽  
pp. 415-424 ◽  
Author(s):  
D. L. Kong ◽  
J. J. Wang ◽  
P. Kardol ◽  
H. F. Wu ◽  
H. Zeng ◽  
...  
Keyword(s):  
Plant Species ◽  
Root Traits ◽  
Root Diameter ◽  
Resource Acquisition ◽  
Root Cortex ◽  
Significant Relationships ◽  
Economic Strategies ◽  
Absorptive Roots ◽  
New Perspective ◽  
N Fractions

Abstract. Plant roots typically vary along a dominant ecological axis, the root economics spectrum, depicting a tradeoff between resource acquisition and conservation. For absorptive roots, which are mainly responsible for resource acquisition, we hypothesized that root economic strategies differ with increasing root diameter. To test this hypothesis, we used seven plant species (a fern, a conifer, and five angiosperms from south China) for which we separated absorptive roots into two categories: thin roots (thickness of root cortex plus epidermis < 247 µm) and thick roots. For each category, we analyzed a range of root traits related to resource acquisition and conservation, including root tissue density, different carbon (C), and nitrogen (N) fractions (i.e., extractive, acid-soluble, and acid-insoluble fractions) as well as root anatomical traits. The results showed significant relationships among root traits indicating an acquisition-conservation tradeoff for thin absorptive roots while no such trait relationships were found for thick absorptive roots. Similar results were found when reanalyzing data of a previous study including 96 plant species. The contrasting economic strategies between thin and thick absorptive roots, as revealed here, may provide a new perspective on our understanding of the root economics spectrum.

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