scholarly journals Treatment of isolated pistils with protease inhibitors overcomes the self-incompatibility response in buckwheat

2007 ◽  
Vol 59 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Jovanka Miljus-Djukic ◽  
Svetlana Radovic ◽  
Vesna Maksimovic
Keyword(s):  
Pollen Tube ◽  
Protease Inhibitors ◽  
Pollen Tube Growth ◽  
Fluorescence Microscope ◽  
Fagopyrum Esculentum ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Fagopyrum Esculentum Moench ◽  
Pepstatin A

Isolated pistils of distylous buckwheat (Fagopyrum esculentum Moench) were treated with protease inhibitors (PMSF, pepstatin A, and antipain). Pistils were cross- or self- pollinated, and growth of pollen tubes was observed under a fluorescence microscope. Treatments with all inhibitors suppressed inhibition of self-pollen tube growth, suggesting that activity of proteases is involved in rejection of self-pollen during the SI response.

Pollen‒pistil interaction in Lilium longiflorum : the role of the pistil in controlling pollen tube growth following cross- and self-pollinations

1982 ◽  
Vol 215 (1198) ◽  
pp. 45-62 ◽  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Active Component ◽  
Lilium Longiflorum ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
System A ◽  
Pollen Tube Elongation

By cytophysiological methods, the self-incompatibility mechanism of the breeding system in Lilium longiflorum has been examined with particular reference to the synthesis, location and nature of the stylar factors involved in the control of pollen tube development. A ‘bioassay’ has been developed by which the effect of stylar extracts on pollen tube elongation may be investigated. With use of this system, a crude fraction of proteins from the stylar fluid has been shown to inhibit pollen tube growth only when protein fractions from ‘self’ styles are used. The proteins of this fraction have been analysed by thin-layer gel electrofocusing. Changes in the profiles thus obtained following selfing and a heat treatment known to inactivate the self-incompatibility response indicate a highly polarized glycoprotein to be an active component of the system. The various ways by which such a glycoprotein could control pollen tube elongation are considered in detail, and these events in Lilium are discussed in the light of our knowledge of other self-incompatibility systems operating in angiosperms.

scholarly journals Seasonal Changes in the Self-Incompatibility and Pollen Tube Growth in Japanese Pears (Pyrus serotina Rehd.)

1985 ◽  
Vol 53 (4) ◽  
pp. 377-382 ◽  
Author(s):  
Shin HIRATSUKA ◽  
Makoto HIROTA ◽  
Eikichi TAKAHASHI ◽  
Naomi HIRATA
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Seasonal Changes ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Pyrus Serotina

scholarly journals Testing of self-(in)compatibility in apricot cultivars from European breeding programmes

2013 ◽  
Vol 40 (No. 2) ◽  
pp. 65-71 ◽  
Author(s):  
D. Milatović ◽  
D. Nikolić ◽  
B. Krška
Keyword(s):  
Fluorescence Microscopy ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Reliable Method ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Breeding Programmes

Self-(in)compatibility was tested in 40 new apricot cultivars from European breeding programmes. Pollen-tube growth in pistils from laboratory pollinations was analysed using the fluorescence microscopy. Cultivars were considered self-compatible if at least one pollen tube reached the ovary in the majority of pistils. Cultivars were considered self- incompatible if the growth of pollen tubes in the style stopped along with formation of characteristic swellings. Of the examined cultivars, 18 were self-compatible and 22 were self-incompatible. Fluorescence microscopy provides a relatively rapid and reliable method to determine self-incompatibility in apricot cultivars.      

Cellular Mechanisms for Pollen Tube Growth Inhibition in Gametophytic Self-incompatibility

2006 ◽  
pp. 201-221 ◽  
Author(s):  
Barend H. J. de Graaf ◽  
Chris Lee ◽  
Bruce A. McClure ◽  
Noni (V. E.) Franklin-Tong
Keyword(s):  
Pollen Tube ◽  
Growth Inhibition ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Cellular Mechanisms
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