scholarly journals Phase Change Related microRNA Profiles in the Plant Regeneration Process of Avocado through Shoot-tip Culture

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Jayeni Hiti-Bandaralage ◽  
◽  
Alice Hayward ◽  
Chris O’Brien ◽  
Umair Ahsan ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Phase Change ◽  
Shoot Tip ◽  
Regeneration Process ◽  
Shoot Tip Culture

scholarly journals Micropropagation of Anubias barteri var. Nana from Shoot Tip Culture and the Analysis of Ploidy Stability

2012 ◽  
Vol 40 (2) ◽  
pp. 148 ◽  
Author(s):  
Kantamaht KANCHANAPOOM ◽  
Panyaros CHUNUI ◽  
Kamnoon KANCHANAPOOM
Keyword(s):  
Plant Regeneration ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Tip Culture ◽  
Ploidy Stability ◽  
Flow Cytometric ◽  
Regenerated Plants ◽  
Mother Plants

Plant regeneration of Anubias barteri var. Nana was achieved through organogenesis in shoot tip cultures. Multiple shoots were induced from cultured shoot tips on a modified MS (Murashige and Skoog, 1962) medium supplemented with BA and kinetin. The maximum green shoot numbers were best obtained on MS medium containing 3 mg/L BA with 5 shoots. Rooting in all regenerated shoots was promoted on MS medium devoid of plant growth regulators or kinetin singly. Acclimatization and survival when transferred to field conditions were shown to be 100% in the regenerated plants. Cytological and flow cytometric analyses of the mother plants and in vitro grown plants derived from 5 years old cultures showed no differences in ploidy level, they were all diploid (2n = 2x = 48) with a 2C peak indicating that ploidy alteration did not occur.

scholarly journals Plant Regeneration of Difficult-to-root Native Wild Japanese Grape, Vitis kiusiana Momiyama, through In vitro Shoot Tip Culture.

1996 ◽  
Vol 13 (2) ◽  
pp. 139-145
Author(s):  
Ryosuke MOCHIOKA ◽  
Motoharu HAMAJI ◽  
Shousaku HORIUCHI ◽  
Hiroshi KUROOKA
Keyword(s):  
Plant Regeneration ◽  
Shoot Tip ◽  
Shoot Tip Culture

Different eradication effects of latent viruses by combining thermotherapy with shoot tip culture or cryotherapy in four apple cultivars

2021 ◽  
Vol 288 ◽  
pp. 110356
Author(s):  
Li Liu ◽  
Xuqiang Chen ◽  
Leiyu Yan ◽  
Yuankai Jin ◽  
Lulong Sun ◽  
...  
Keyword(s):  
Shoot Tip ◽  
Shoot Tip Culture ◽  
Apple Cultivars

scholarly journals Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture

2017 ◽  
Vol 16 (2) ◽  
Author(s):  
M.J.S. Carvalho ◽  
E.J. Oliveira ◽  
A.S. Souza ◽  
J.S. Pereira ◽  
M.S.A.S. Diamantino ◽  
...  
Keyword(s):  
Shoot Tip ◽  
Shoot Tip Culture

scholarly journals In vitro Mass Propagation of a Ground Orchid - Phaius tancarvilleae (L'Her.) Blume through Shoot Tip Culture

2011 ◽  
Vol 21 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha
Keyword(s):  
High Frequency ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Mass Propagation ◽  
Shoot Tip Culture ◽  
Shoot Tip Explants ◽  
Direct Shoot

High frequency direct shoot proliferation was induced from the shoot tip explants derived from the in vitro grown seedlings of a critically endangered and horticulturally important ground orchid Phaius tancarvilleae (L'Her) Blume. Shoot tip explants cultured on solidified MS with alone or combination of various concentrations of NAA and BAP produced shoots and multiple shoots. The maximum number of healthy shoots was observed on MS with BAP (1.0 mg/l) with an average of 13.3 shoots per culture in 20 weeks; where shoot multiplication was initiated after 4 weeks of culture. Regenerated shoots rooted on MS with various concentrations of NAA, IAA, IBA. MS with NAA (0.5 mg/l) was the most appropriate condition for rooting. The well developed in vitro rooted plantlets were hardened successfully in the potting mixture containing cocopeat and sphagnum moss in the ratio of 2 : 1.   Key words: Mass propagation, Phaius tancarvilleae, shoot multiplication   D. O. I. 10.3329/ptcb.v21i2.10241   Plant Tissue Cult. & Biotech. 21(2): 181-188, 2011 (December)

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