scholarly journals 3D culture of murine adipose-derived multipotent mesenchymal stromal cells in hydrogel based on carbomer 974P

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
V. Kyryk ◽  
O. Kuchuk ◽  
A. Mamchur ◽  
A. Ustymenko ◽  
T. Lutsenko ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Culture Media ◽  
Three Dimensional ◽  
3D Culture ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Term Survival ◽  
Hydrogel Scaffolds ◽  
3D Cultivation

Actual issues during tissue regeneration are to ensure the survival of transplanted cells at the site of their application and further activity, especially in case of local pathological alterations such as inflammation and ischemia. For this purpose, the matrices that can not only fill the defects of tissues, but also be scaffolds for cells are developed.The aim of this study was to evaluate the effectiveness of 3D cultivation of murine adipose-derived multipotent mesenchymal stromal cells (MSMCs) in hydrogel based on carbomer 974P.Materials and methods. MSMCs were obtained from the adipose tissue of FVB-Cg-Tg(GFPU)5Nagy/J mice transgenic for GFP gene. The cells were phenotyped by flow cytometry and directly differentiated into osteogenic and adipogenic direction to confirm multipotent phenotype. MMSCs were cultured and directly differentiated into osteogenic direction in three-dimensional hydrogel scaffolds. For hydrogel preparation we used carbomer 974P with composition of glycerol, propylene glycol, triethylamine and agarose in original proportion.Results. The three-dimensional hydrogel based on carbomer 974P for the further engraftment with MMSCs was obtained. Modified protocols for the preparation of hydrogels based on carbomer and agarose and their rehydration by culture media for the 3D cultivation of adipose-derived MMSCs have been developed. The optimal concentration of MSMCs and the injection method for engraftment of hydrogels of the required form and size are selected. It was shown that adipose-derived MMSCs in 3D carbomer hydrogel preserve the potential of directed osteogenic differentiation.Conclusion. Three-dimensional hydrogel based on carbomer 974P is capable to support cells, provide the necessary cytoarchitectonics, maintain intercellular interactions, which can promote further long-term survival and specialization of graft.

scholarly journals Long-term Engraftment of Multipotent Mesenchymal Stromal Cells That Differentiate to Form Myogenic Cells in Dogs With Duchenne Muscular Dystrophy

2012 ◽  
Vol 20 (1) ◽  
pp. 168-177 ◽  
Author(s):  
Yuko Nitahara-Kasahara ◽  
Hiromi Hayashita-Kinoh ◽  
Sachiko Ohshima-Hosoyama ◽  
Hironori Okada ◽  
Michiko Wada-Maeda ◽  
...  
Keyword(s):  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Myogenic Cells

scholarly journals Three-dimensional cartilage-derived construct derived from multipotent mesenchymal stromal cells

2020 ◽  
Vol 22 ◽  
pp. 02014
Author(s):  
Oleg Makeyev ◽  
Arteom Korotkov ◽  
Svetlana Kostyukova ◽  
Daria Sichkar ◽  
Elizaveta Yakovleva
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Three Dimensional ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Cartilage Tissue ◽  
Cartilage Surface ◽  
Initial Stage ◽  
Conventional Drug ◽  
Surgical Methods ◽  
Further Development

Damage to the cartilage surface of the joints is a common pathology. Conventional drug therapies are effective only at the initial stage of the disease and only slow down the further development of the disease. In subsequent stages, it is necessary to apply surgical methods from replacing a defect to complete prosthetics of a damaged joint. In order to replace defects in cartilage tissue, a three-dimensional bioequivalent was created based on autologous multipotent mesenchymal stromal cells.

scholarly journals Alpha-1 Antitrypsin-Expressing Mesenchymal Stromal Cells Confer a Long-Term Survival Benefit in a Mouse Model of Lethal GvHD

2019 ◽  
Vol 27 (8) ◽  
pp. 1436-1451
Author(s):  
Sabine Geiger ◽  
Emrah I. Ozay ◽  
Ulf Geumann ◽  
Marina K. Hereth ◽  
Terese Magnusson ◽  
...  
Keyword(s):  
Mouse Model ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Survival Benefit ◽  
Term Survival ◽  
Long Term Survival ◽  
Alpha 1 Antitrypsin

scholarly journals Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Olga Schmal ◽  
Jan Seifert ◽  
Tilman E. Schäffer ◽  
Christina B. Walter ◽  
Wilhelm K. Aicher ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Ex Vivo ◽  
Three Dimensional ◽  
3D Culture ◽  
Hanging Drop ◽  
Mixed Cell ◽  
Hematopoietic Stem ◽  
Extracellular Matrix Components ◽  
Stem And Progenitor Cells

Efficientex vivoexpansion of hematopoietic stem cells with a concomitant preservation of stemness and self-renewal potential is still an unresolved ambition. Increased numbers of methods approaching this issue using three-dimensional (3D) cultures were reported. Here, we describe a simplified 3D hanging drop model for the coculture of cord blood-derived CD34+hematopoietic stem and progenitor cells (HSPCs) with bone marrow-derived mesenchymal stromal cells (MSCs). When seeded as a mixed cell suspension, MSCs segregated into tight spheroids. Despite the high expression of niche-specific extracellular matrix components by spheroid-forming MSCs, HSPCs did not migrate into the spheroids in the initial phase of coculture, indicating strong homotypic interactions of MSCs. After one week, however, HSPC attachment increased considerably, leading to spheroid collapse as demonstrated by electron microscopy and immunofluorescence staining. In terms of HSPC proliferation, the conventional 2D coculture system was superior to the hanging drop model. Furthermore, expansion of primitive hematopoietic progenitors was more favored in 2D than in 3D, as analyzed in colony-forming assays. Conclusively, our data demonstrate that MSCs, when arranged with a spread (monolayer) shape, exhibit better HSPC supportive qualities than spheroid-forming MSCs. Therefore, 3D systems are not necessarily superior to traditional 2D culture in this regard.

scholarly journals Clonal chromosomal and genomic instability during human multipotent mesenchymal stromal cells long-term culture

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0192445 ◽  
Author(s):  
Victoria Nikitina ◽  
Tatiana Astrelina ◽  
Vladimir Nugis ◽  
Aleksandr Ostashkin ◽  
Tatiana Karaseva ◽  
...  
Keyword(s):  
Genomic Instability ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Long Term Culture
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