Use of human fetal liver cells for treatment of patients with lower limb peripheral artery disease

2014 ◽  
Vol 2 (1) ◽  
pp. 10-13
Author(s):  
R. Salyutin ◽  
D. Dombrowski ◽  
M. Komarov ◽  
N. Sokolov ◽  
S. Palyanitsya ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Lower Limb ◽  
Fetal Liver ◽  
Life Quality ◽  
Liver Cells ◽  
Surgical Reconstruction ◽  
Clinical Effects ◽  
Doppler Flowmetry ◽  
Human Fetal Liver ◽  
Fetal Liver Cells

In the group of patients (n = 21, mean age 54 ± 5.8 years) with chronic lower limb ischemia stage IIB who were non-liable for reconstructiverestoration surgery, we have established positive clinical effects of local transplantation of human fetal liver progenitor cells. Complex examination following 1, 3, 6 and 12 months after transplantation included duplex scanning of limb arteries, x-ray contrast arteriography and laser Doppler flowmetry as well as measuring pain-free walking and evaluating life quality based on individual questionnaire data.Owing to the transplant “Cryopreserved human fetal liver progenitor cells” the patients demonstrated stable increase of life quality index and pain-free walking as well as improvement of general health allowing assign them to the group of patients with lower ischemia stage,  quicker social rehabilitation and lesser risk of disabling surgery (р < 0.05). Also, there were observations of improved microcirculation in the ischemic extremities owing to activation of endothelium-independent mechanisms of vasodilatation, reduced myotonus and neurotonus of the pre-capillaries and improved endothelium-dependent influence on the microhaemodynamic and, hence, an increased reserve capillary blood flow (p < 0.05).Analysis of the obtained results indicates prospects and effectiveness of using fetal liver cells transplantation in the patients who are not liable for surgical reconstruction of the vascular bed.

scholarly journals Comparison of Basal Gene Expression and Induction of CYP3As in HepG2 and Human Fetal Liver Cells

2007 ◽  
Vol 30 (11) ◽  
pp. 2091-2097 ◽  
Author(s):  
Masataka Maruyama ◽  
Tamihide Matsunaga ◽  
Eri Harada ◽  
Shigeru Ohmori
Keyword(s):  
Gene Expression ◽  
Fetal Liver ◽  
Liver Cells ◽  
Human Fetal Liver ◽  
Fetal Liver Cells ◽  
Human Fetal Liver Cells

scholarly journals Mechanisms of CYP3A Induction by Glucocorticoids in Human Fetal Liver Cells

2012 ◽  
Vol 27 (6) ◽  
pp. 653-657 ◽  
Author(s):  
Tamihide Matsunaga ◽  
Masataka Maruyama ◽  
Tsutomu Matsubara ◽  
Kiyoshi Nagata ◽  
Yasushi Yamazoe ◽  
...  
Keyword(s):  
Fetal Liver ◽  
Liver Cells ◽  
Human Fetal Liver ◽  
Fetal Liver Cells ◽  
Human Fetal Liver Cells

Expansion of mouse hematopoietic stem/progenitor cells in three-dimensional cocultures on growth-suppressed stromal cell layer

2019 ◽  
Vol 42 (7) ◽  
pp. 374-379 ◽  
Author(s):  
Hirotoshi Miyoshi ◽  
Chiaki Sato ◽  
Yuichiro Shimizu ◽  
Misa Morita
Keyword(s):  
Progenitor Cells ◽  
Mitomycin C ◽  
Stromal Cells ◽  
Fetal Liver ◽  
Three Dimensional ◽  
Hematopoietic Cells ◽  
Liver Cells ◽  
Hematopoietic Progenitor ◽  
Hematopoietic Stem ◽  
Fetal Liver Cells

With the aim of establishing an effective method to expand hematopoietic stem/progenitor cells for application in hematopoietic stem cell transplantation, we performed ex vivo expansion of hematopoietic stem/progenitor cells derived from mouse fetal liver cells in three-dimensional cocultures with stromal cells. In these cocultures, stromal cells were first cultured within three-dimensional scaffolds to form stromal layers and then fetal liver cells containing hematopoietic cells were seeded on these scaffolds to expand the hematopoietic cells over the 2 weeks of coculture in a serum-containing medium without the addition of cytokines. Prior to coculture, stromal cell growth was suppressed by treatment with the DNA synthesis inhibitor mitomycin C, and its effect on hematopoietic stem/progenitor cell expansion was compared with that in control cocultures in which fetal liver cells were cocultured with three-dimensional freeze-thawed stromal cells. After coculture with mitomycin C-treated stromal cells, we achieved a several-fold expansion of the primitive hematopoietic cells (c-kit+hematopoietic progenitor cells >7.8-fold, and CD34+hematopoietic stem/progenitor cells >3.5-fold). Compared with control cocultures, expansion of hematopoietic stem/progenitor cells tended to be lower, although that of hematopoietic progenitor cells was comparable. Thus, our results suggest that three-dimensional freeze-thawed stromal cells have higher potential to expand hematopoietic stem/progenitor cells compared with mitomycin C-treated stromal cells.

scholarly journals Expression of paramyxovirus V proteins promotes replication and spread of hepatitis C virus in cultures of primary human fetal liver cells

Hepatology ◽  
2011 ◽  
Vol 54 (6) ◽  
pp. 1901-1912 ◽  
Author(s):  
Linda Andrus ◽  
Svetlana Marukian ◽  
Christopher T. Jones ◽  
Maria Teresa Catanese ◽  
Timothy P. Sheahan ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Fetal Liver ◽  
Liver Cells ◽  
Human Fetal Liver ◽  
Fetal Liver Cells ◽  
Human Fetal Liver Cells

α-Fetoprotein production and erythropoiesis in cell cultures of human fetal liver

1977 ◽  
Vol 55 (5) ◽  
pp. 571-575 ◽  
Author(s):  
L. F. Congote ◽  
F. Bruno ◽  
S. Solomon
Keyword(s):  
Cell Function ◽  
Fetal Liver ◽  
Calf Serum ◽  
Liver Cells ◽  
Velocity Sedimentation ◽  
Erythroid Cell ◽  
Erythroid Cells ◽  
Human Fetal Liver ◽  
Fetal Liver Cells ◽  
Human Fetal Liver Cells

α-Fetoprotein and the synthesis of heme associated with hemoglobin were measured simultaneously in short-term cultures of human fetal liver cells to correlate the relationship of α-fetoprotein to erythroid cell function. Both synthetic processes decreased exponentially during the first 5 days of culture. The use of media supplemented with different batches of fetal calf serum and porcine portal vein serum indicated that the optimal conditions for the production of α-fetoprotein were different from those required for the synthesis of heme associated with hemoglobin. Moreover, the α-fetoprotein-producing cells could be separated from erythroid cells after velocity sedimentation in Ficoll gradients. Although it is well known that erythropoiesis and α-fetoprotein production occur simultaneously during ontogenesis, α-fetoprotein itself (0.01–100 μg/ml) did not stimulate heme synthesis in liver erythroid cells. Erythropoietin did not stimulate α-fetoprotein production. It is concluded that there is no cause–effect relationship between α-fetoprotein production and erythroid cell function in human fetal liver cells and that the two processes occur independently in different cell types.

scholarly journals Phenotypic and Functional Evidence for the Expression of CD4 by Hematopoietic Stem Cells Isolated From Human Fetal Liver

Blood ◽  
1997 ◽  
Vol 89 (4) ◽  
pp. 1364-1375 ◽  
Author(s):  
Marcus O. Muench ◽  
Maria Grazia Roncarolo ◽  
Reiko Namikawa
Keyword(s):  
Stem Cells ◽  
Fetal Liver ◽  
Liver Cells ◽  
Proliferative Potential ◽  
Erythroid Progenitors ◽  
Hematopoietic Stem ◽  
Human Fetal Liver ◽  
Fetal Bone ◽  
Fetal Stem Cells ◽  
Fetal Liver Cells

Abstract Expression of the CD4 antigen was observed on human fetal liver, fetal bone marrow (BM), and umbilical cord blood progenitors expressing high levels of CD34. Using clonal and liquid-culture assays, CD4+ CD34++ Lin− (lineage = CD3, CD8, CD10, CD14, CD15, CD16, CD19, CD20, and glycophorin A) fetal liver progenitors were found to have a greater proliferative potential than CD4− CD34++ Lin− progenitors, whereas the CD4− fraction was more enriched for erythroid progenitors. Both the CD4+ and the CD4− progenitor subpopulations also gave rise to multilineage engraftment upon transplantation into human fetal bone fragments, supportive of B-lymphoid and myeloid growth, or into human fetal thymic fragments, supportive of T-cell growth, implanted in scid/scid (SCID) mice. However, in SCID-hu mice transplanted with graded doses of donor cells ranging from 2.0 × 102 to 2.0 × 104 cells, BM reconstitution by the CD4+ fraction of CD34++ Lin− cells was more frequent than by the CD4− fraction when low numbers of cells were injected. These functional data strongly suggest that stem cells reside among CD4+ CD34++ Lin− fetal liver cells. This hypothesis was further supported by the observations that CD4+ CD34++ Lin− fetal liver cells were enriched for CDw90+ (Thy-1), CD117+ (kit), CD123+, HLA-DR+, CD7−, CD38−, CD45RA−, CD71−, CD115− (fms), and rhodamine 123dull cells, a phenotypic profile believed to represent fetal stem cells. Furthermore, all CD4+ CD34++ Lin− fetal liver cells also expressed CD13 and CD33.

scholarly journals Globin gene expression in erythroid human fetal liver cells.

1989 ◽  
Vol 83 (3) ◽  
pp. 1032-1038 ◽  
Author(s):  
T J Ley ◽  
K A Maloney ◽  
J I Gordon ◽  
A L Schwartz
Keyword(s):  
Gene Expression ◽  
Fetal Liver ◽  
Globin Gene ◽  
Liver Cells ◽  
Human Fetal Liver ◽  
Fetal Liver Cells ◽  
Human Fetal Liver Cells ◽  
Globin Gene Expression
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