scholarly journals Acellular matrix as a substrate for tissue-engineered graft of heart valve

2013 ◽  
Vol 1 (1) ◽  
pp. 52-55 ◽  
Author(s):  
A. Popandopulo ◽  
M. Petrova
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Heart Valve ◽  
Heart Valves ◽  
Three Dimensional ◽  
Tissue Scaffolds ◽  
The Body ◽  
Heart Regeneration ◽  
Acellular Matrix ◽  
Living Materials

In many cases heart valve prosthetics is the only solution to save patient’s life. All mechanical prosthetics currently used are not able to perform function in the body fully because non-living materials are used for their production. Tissue engineering provides the reconstruction of viable valves using stem cells. Acellularized three-dimensional tissue scaffolds as a matrix for autologous cells do improve function of heart valves and promote heart regeneration.

Tissue Engineering of Heart Valves: Formation of a Three-Dimensional Tissue Using Porcine Heart Valve Cells

ASAIO Journal ◽  
2002 ◽  
Vol 48 (6) ◽  
pp. 586-591 ◽  
Author(s):  
Markus Rothenburger ◽  
Wolfgang Völker ◽  
Peter Vischer ◽  
Elmar Berendes ◽  
Birgit Glasmacher ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Heart Valve ◽  
Heart Valves ◽  
Three Dimensional ◽  
Porcine Heart

Tissue Engineering of Pulmonary Heart Valves on Allogenic Acellular Matrix Conduits

Circulation ◽  
2000 ◽  
Vol 102 (suppl_3) ◽  
Author(s):  
Gustav Steinhoff ◽  
Ulrich Stock ◽  
Najibulla Karim ◽  
Heike Mertsching ◽  
Adine Timke ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Heart Valve ◽  
Heart Valves ◽  
Sheep Model ◽  
Control Valves ◽  
Acellular Matrix ◽  
Valve Tissue

Background —Tissue engineering using in vitro–cultivated autologous vascular wall cells is a new approach to biological heart valve replacement. In the present study, we analyzed a new concept to process allogenic acellular matrix scaffolds of pulmonary heart valves after in vitro seeding with the use of autologous cells in a sheep model. Methods and Results —Allogenic heart valve conduits were acellularized by a 48-hour trypsin/EDTA incubation to extract endothelial cells and myofibroblasts. The acellularization procedure resulted in an almost complete removal of cells. After that procedure, a static reseeding of the upper surface of the valve was performed sequentially with autologous myofibroblasts for 6 days and endothelial cells for 2 days, resulting in a patchy cellular restitution on the valve surface. The in vivo function was tested in a sheep model of orthotopic pulmonary valve conduit transplantation. Three of 4 unseeded control valves and 5 of 6 tissue-engineered valves showed normal function up to 3 months. Unseeded allogenic acellular control valves showed partial degeneration (2 of 4 valves) and no interstitial valve tissue reconstitution. Tissue-engineered valves showed complete histological restitution of valve tissue and confluent endothelial surface coverage in all cases. Immunohistological analysis revealed cellular reconstitution of endothelial cells (von Willebrand factor), myofibroblasts (α-actin), and matrix synthesis (procollagen I). There were histological signs of inflammatory reactions to subvalvar muscle leading to calcifications, but these were not found in valve and pulmonary artery tissue. Conclusions —The in vitro tissue-engineering approach using acellular matrix conduits leads to the in vivo reconstitution of viable heart valve tissue.

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

Tissue Engineering of Pulmonary Heart Valves on Allogenic Acellular Matrix Conduits : In Vivo Restoration of Valve Tissue

Circulation ◽  
2000 ◽  
Vol 102 (Supplement 3) ◽  
pp. III-50-III-55 ◽  
Author(s):  
G. Steinhoff ◽  
U. Stock ◽  
N. Karim ◽  
H. Mertsching ◽  
A. Timke ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Heart Valves ◽  
Acellular Matrix ◽  
Valve Tissue

Transplantation of collagen sponge-based three-dimensional neural stem cells cultured in the RCCS system facilitate locomotor functional recovery in spinal cord injury animals

2022 ◽  
Author(s):  
Jianwu Dai ◽  
Yunlong Zou ◽  
Yanyun Yin ◽  
Zhifeng Xiao ◽  
Yannan Zhao ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
Three Dimensional ◽  
Collagen Sponge ◽  
Cellular Functions ◽  
Cord Injury ◽  
Cells Cultured

Numerous studies have indicated that microgravity induces various changes in the cellular functions of neural stem cells (NSCs), and the use of microgravity to culture tissue engineering seed cells for...

Dental Pulp Stem Cells for Tissue Engineered Heart Valve

2020 ◽  
Author(s):  
Soontaree Petchdee ◽  
Wilairat Chumsing ◽  
Suruk Udomsom ◽  
Kittiya Thunsiri
Keyword(s):  
Stem Cells ◽  
Mitral Valve ◽  
Heart Valve ◽  
Heart Valves ◽  
Cell Types ◽  
Tensile Tests ◽  
Deciduous Teeth ◽  
Essential Information ◽  
Acquired Heart Disease ◽  
Tissue Engineered Heart Valves

Myxomatous mitral valve degeneration is the most acquired heart disease in dogs. To reduce the clinical progression of mitral valve degeneration and achieve the hemodynamic outcomes, many medical or surgical treatments have been motivated. The objectives of this study is to investigate the suitability of puppy deciduous teeth stem cells as a cell source for tissue engineered heart valves in dog with degenerative valve disease. Puppy deciduous teeth stem cells (pDSCs) were seeded on the scaffolds which made from polylactic acid (PLA), polycaprolactone (PLC) and silicone. The mechanical properties of the tissue engineered heart valves leaflets were characterized by biaxial tensile tests. Results showed that, deciduous teeth stem cells capable of differentiating into a variety of cell types. However, the ability of puppy deciduous teeth stem cells to differentiate declined with increasing passage number which correspond to the number of protein surface marker detection have been shown to decrease substantially by the fifth passage. PLA scaffold is significantly higher tensile strength than other materials. However, silicone showed the highest flaccidity. The results from this study may provide high regenerative capability and the essential information for future directions of heart valve tissue engineering.

scholarly journals The Influence of Astaxanthin on the Proliferation of Adipose-derived Mesenchymal Stem Cells in Gelatin-Methacryloyl (GelMA) Hydrogels

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2416 ◽  
Author(s):  
Bo Young Choi ◽  
Elna Paul Chalisserry ◽  
Myoung Hwan Kim ◽  
Hyun Wook Kang ◽  
Il-Whan Choi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cellular Proliferation ◽  
Three Dimensional ◽  
Confocal Imaging ◽  
Alamar Blue Assay ◽  
Ratio Test ◽  
Alamar Blue ◽  
Chemical Structures

Recently, astaxanthin, a red lipophilic pigment belonging to the xanthophyllic family of carotenoids, has shown the feasibility of its uses in tissue engineering and regenerative medicine, due to its excellent antioxidant activities and its abilities to enhance the self-renewal potency of stem cells. In this study, we demonstrate the influence of astaxanthin on the proliferation of adipose-derived mesenchymal stem cells in tissue-engineered constructs. The tissue engineered scaffolds were fabricated using photopolymerizable gelatin methacryloyl (GelMA) with different concentrations of astaxanthin. The effects of astaxanthin on cellular proliferation in two-dimensional environments were assessed using alamar blue assay and reverse transcription polymerase chain reaction (RT-PCR). Then, rheological properties, chemical structures and the water absorption of the fabricated astaxanthin-incorporated GelMA hydrogels were characterized using NMR analysis, rheological analysis and a swelling ratio test. Finally, the influence in three-dimensional environments of astaxanthin-incorporated GelMA hydrogels on the proliferative potentials of adipose-derived stem cells was assessed using alamar blue assay and the confocal imaging with Live/dead staining. The experimental results of the study indicate that an addition of astaxanthin promises to induce stem cell potency via proliferation, and that it can be a useful tool for a three-dimensional culture system and various tissue engineering applications.

scholarly journals A Customizable, Low-Cost Perfusion System for Sustaining Tissue Constructs

2018 ◽  
Vol 23 (6) ◽  
pp. 592-598
Author(s):  
Brian J. O’Grady ◽  
Jason X. Wang ◽  
Shannon L. Faley ◽  
Daniel A. Balikov ◽  
Ethan S. Lippmann ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Large Scale ◽  
Low Cost ◽  
Three Dimensional ◽  
Tissue Scaffolds ◽  
Perfusion System ◽  
Pump System ◽  
Tissue Constructs ◽  
Engineered Tissue

The fabrication of engineered vascularized tissues and organs requiring sustained, controlled perfusion has been facilitated by the development of several pump systems. Currently, researchers in the field of tissue engineering require the use of pump systems that are in general large, expensive, and generically designed. Overall, these pumps often fail to meet the unique demands of perfusing clinically useful tissue constructs. Here, we describe a pumping platform that overcomes these limitations and enables scalable perfusion of large, three-dimensional hydrogels. We demonstrate the ability to perfuse multiple separate channels inside hydrogel slabs using a preprogrammed schedule that dictates pumping speed and time. The use of this pump system to perfuse channels in large-scale engineered tissue scaffolds sustained cell viability over several weeks.

scholarly journals Human Mesenchymal Stem Cells Reendothelialize Porcine Heart Valve Scaffolds: Novel Perspectives in Heart Valve Tissue Engineering

2015 ◽  
Vol 4 (1) ◽  
pp. 288-297 ◽  
Author(s):  
Paola Lanuti ◽  
Francesco Serafini ◽  
Laura Pierdomenico ◽  
Pasquale Simeone ◽  
Giuseppina Bologna ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Heart Valve ◽  
Human Mesenchymal Stem Cells ◽  
Porcine Heart ◽  
Valve Tissue ◽  
Heart Valve Tissue ◽  
Heart Valve Tissue Engineering

scholarly journals A Three-Dimensional Collagen-Elastin Scaffold for Heart Valve Tissue Engineering

2018 ◽  
Vol 5 (3) ◽  
pp. 69 ◽  
Author(s):  
Xinmei Wang ◽  
Mir Ali ◽  
Carla Lacerda
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Heart Valves ◽  
Current Knowledge ◽  
Three Dimensional ◽  
Stimuli Responsive ◽  
Integrin Β1 ◽  
Mesenchymal Transition ◽  
3D Collagen

Since most of the body’s extracellular matrix (ECM) is composed of collagen and elastin, we believe the choice of these materials is key for the future and promise of tissue engineering. Once it is known how elastin content of ECM guides cellular behavior (in 2D or 3D), one will be able to harness the power of collagen-elastin microenvironments to design and engineer stimuli-responsive tissues. Moreover, the implementation of such matrices to promote endothelial-mesenchymal transition of primary endothelial cells constitutes a powerful tool to engineer 3D tissues. Here, we design a 3D collagen-elastin scaffold to mimic the native ECM of heart valves, by providing the strength of collagen layers, as well as elasticity. Valve interstitial cells (VICs) were encapsulated in the collagen-elastin hydrogels and valve endothelial cells (VECs) cultured onto the surface to create an in vitro 3D VEC-VIC co-culture. Over a seven-day period, VICs had stable expression levels of integrin β1 and F-actin and continuously proliferated, while cell morphology changed to more elongated. VECs maintained endothelial phenotype up to day five, as indicated by low expression of F-actin and integrin β1, while transformed VECs accounted for less than 7% of the total VECs in culture. On day seven, over 20% VECs were transformed to mesenchymal phenotype, indicated by increased actin filaments and higher expression of integrin β1. These findings demonstrate that our 3D collagen-elastin scaffolds provided a novel tool to study cell-cell or cell-matrix interactions in vitro, promoting advances in the current knowledge of valvular endothelial cell mesenchymal transition.

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