scholarly journals Characterization of Electrospun Silk Fibroin Scaffolds for Bone Tissue Engineering: A Review

TecnoLógicas ◽  
2020 ◽  
Vol 23 (49) ◽  
pp. 33-51
Author(s):  
Mónica Liliana Mejía Suaza ◽  
Maria Elena Moncada ◽  
Claudia Patricia Ossa-Orozco
Keyword(s):  
Tissue Engineering ◽  
Tensile Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Thermal Characteristics ◽  
Future Research ◽  
Fiber Morphology ◽  
Scanning Calorimetry ◽  
Electron Microscopic

Silk Fibroin (SF) is a natural polymer obtained from the Bombyx mori silkworm. It has been used in bone tissue engineering thanks to its favorable biocompatibility, adhesion, low biodegradability, and tensile strength properties. Electrospinning is a technique to develop nanofibers. It uses high voltages to convert polymer solutions into porous nanostructured scaffolds with a good ratio between superficial area and volume. In this paper, we examine the effect of the electrospinning parameters on fiber morphology once the spun fibers have been treated. In addition, we present different physicochemical characterizations of electrospun SF scaffolds such as their morphology (via Scanning Electron Microscopic—SEM—), crystalline structure (via Fourier Transform Infrared—FTIR—spectroscopy and X-Ray Diffraction—XRD—), thermal characteristics (via Differential Scanning Calorimetry—DSC—and Thermogravimetric Analysis—TGA—), and mechanical properties (tensile strength). Finally, we discuss the potential applications and impacts of electrospun SF in bone tissue engineering and future research trends.

PLGA+HA/βTCP scaffold incorporating simvastatin: a promising biomaterial for bone tissue engineering

2020 ◽  
Author(s):  
Mariane Beatriz Sordi ◽  
Ariadne Cristiane Cabral da Cruz ◽  
Águedo Aragones ◽  
Mabel Mariela Rodríguez Cordeiro ◽  
Ricardo de Souza Magini
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Thermal Analyses ◽  
Chemical Properties ◽  
Biological Properties ◽  
Scanning Calorimetry ◽  
Evaporation Technique ◽  
Porosity Analysis ◽  
Biphasic Ceramic

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite / β-tricalcium phosphate (PLGA+HA/βTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% βTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m:m) of this medication. Scaffolds were synthesized in a cylindric-shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in PBS at 37 °C under constant stirring for 7, 14, 21, and 28 days. Non-degraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro, meso, and micropores in the polymer structure with HA/βTCP particles homogeneously dispersed. Chemical and thermal analyses presented very similar results for both PLGA+HA/βTCP and PLGA+HA/βTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/βTCP and PLGA+HA/βTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/βTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.

scholarly journals A Bibliometric Analysis of the Global Trend of Using Alginate, Gelatine, and Hydroxyapatite for Bone Tissue Regeneration Applications

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 647
Author(s):  
Mohamed Saiful Firdaus Hussin ◽  
Aludin Mohd Serah ◽  
Khairul Azri Azlan ◽  
Hasan Zuhudi Abdullah ◽  
Maizlinda Izwana Idris ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bibliometric Analysis ◽  
Research Work ◽  
The United States ◽  
Research Area ◽  
Bone Tissue Regeneration ◽  
Future Research ◽  
Starting Point

Collecting information from previous investigations and expressing it in a scientometrics study can be a priceless guide to getting a complete overview of a specific research area. The aim of this study is to explore the interrelated connection between alginate, gelatine, and hydroxyapatite within the scope of bone tissue and scaffold. A review of traditional literature with data mining procedures using bibliometric analyses was considered to identify the evolution of the selected research area between 2009 and 2019. Bibliometric methods and knowledge visualization technologies were implemented to investigate diverse publications based on the following indicators: year of publication, document type, language, country, institution, author, journal, keyword, and number of citations. An analysis using a bibliometric study found that 7446 papers were located with the keywords “bone tissue” and “scaffold”, and 1767 (alginate), 185 (gelatine), 5658 (hydroxyapatite) papers with those specific sub keywords. The number of publications that relate to “tissue engineering” and bone more than doubled between 2009 (1352) and 2019 (2839). China, the United States and India are the most productive countries, while Sichuan University and the Chinese Academy of Science from China are the most important institutions related to bone tissue scaffold. Materials Science and Engineering C is the most productive journal, followed by the Journal of Biomedical Materials Research Part A. This paper is a starting point, providing the first bibliometric analysis study of bone tissue and scaffold considering alginate, gelatine and hydroxyapatite. A bibliometric analysis would greatly assist in giving a scientific insight to support desired future research work, not only associated with bone tissue engineering applications. It is expected that the analysis of alginate, gelatine and hydroxyapatite in terms of 3D bioprinting, clinical outcomes, scaffold architecture, and the regenerative medicine approach will enhance the research into bone tissue engineering in the near future. Continued studies into these research fields are highly recommended.

Development of silk fibroin/nanohydroxyapatite composite hydrogels for bone tissue engineering

2015 ◽  
Vol 67 ◽  
pp. 66-77 ◽  
Author(s):  
Marta Ribeiro ◽  
Mariana A. de Moraes ◽  
Marisa M. Beppu ◽  
Mónica P. Garcia ◽  
Maria H. Fernandes ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Composite Hydrogels

Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach

2021 ◽  
Vol 17 (1) ◽  
pp. 015003
Author(s):  
Lya Piaia ◽  
Simone S Silva ◽  
Joana M Gomes ◽  
Albina R Franco ◽  
Emanuel M Fernandes ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Cellular Proliferation ◽  
Mechanical Performance ◽  
Tissue Growth ◽  
Polymeric Scaffolds ◽  
Bioactive Agents

Abstract Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth. By introducing scaffolds that can be autografts and/or allografts, tissue engineering provides new approaches to manage the major burdens involved in this process. Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions. The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering. Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60–170 μm, and a stiffness ranging from 1 to 2 MPa. Furthermore, when cultured with MC3T3 cells, the scaffolds were able to form apatite crystals after 21 d; and they were able to support cell growth and proliferation up to 14 d of culture. Besides, cellular proliferation was higher on the scaffolds coated with silk. These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering.

Silk Fibroin-Based Scaffold for Bone Tissue Engineering

2018 ◽  
pp. 371-387 ◽  
Author(s):  
Joo Hee Choi ◽  
Do Kyung Kim ◽  
Jeong Eun Song ◽  
Joaquim Miguel Oliveira ◽  
Rui Luis Reis ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin

scholarly journals Preparation and biological properties of silk fibroin/nano-hydroxyapatite/graphene oxide scaffolds with an oriented channel-like structure

RSC Advances ◽  
2020 ◽  
Vol 10 (17) ◽  
pp. 10118-10128 ◽  
Author(s):  
Lu Wang ◽  
Min Fang ◽  
Yijing Xia ◽  
Jiaxin Hou ◽  
Xiaoru Nan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Biological Properties ◽  
Hybrid Scaffold

A novel SF/nHAp/GO hybrid scaffold with oriented channel-like structure in bone tissue engineering.

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