Isolation and Identification of Intestinal parasites and protozoa from Flesh flies Sarcophaga africa in Baghdad

2013 ◽  
Vol 16 (3) ◽  
pp. 214-223
Author(s):  
Afkar M. Hadi ◽  
Keyword(s):  
Intestinal Parasites ◽  
Isolation And Identification ◽  
Flesh Flies

scholarly journals Isolation and Identification of different Intestinal Helminthes Groups from Stray Cats in Urban City of Kirkuk

2021 ◽  
Vol 15 (7) ◽  
pp. 1916-1921
Author(s):  
Husain F. Hassan ◽  
Sabreen Y. Gaeib
Keyword(s):  
Infection Rate ◽  
Intestinal Parasites ◽  
Toxocara Cati ◽  
Isolation And Identification ◽  
Urban City ◽  
Stray Cats ◽  
Dipylidium Caninum ◽  
Taenia Taeniaeformis ◽  
First Time

In this study, 25 stray cats were caught from the urban city of Kirkuk hunted using specially designed traps. Cats were dissected and examined in order to investigate its infection with intestinal parasites. Our results indicate that all stray cats were infected with at least one type of helminthes. Three types of Trematodes were identified: Echinochasmus, Heterophyes and , Prohemistomum with infection rate of 4% for each type. Such species were identified for the first time in stray cats in the Iraq. Moreover, four types of cestodes were identified: Diplopylidium, Dipylidium caninum, Joyeuxiella and Taenia taeniaeformis with infection rate of 24% 48%, 16% and 12%, respectively. Nematodes of Physaloptera preputalis, Toxocara cati and Toxoascaris leonina were also found. The infection rate was 4% ,28% and 8% for each type, respectively. Keywords: intestinal helminthes, stray cats, Trematodes.

scholarly journals Isolation and Identification of Cryptosporidium sp. by Reverse Osmosis System of Tap water in Baghdad

2014 ◽  
Vol 11 (2) ◽  
pp. 894-899
Author(s):  
Baghdad Science Journal
Keyword(s):  
Public Health ◽  
Drinking Water ◽  
Reverse Osmosis ◽  
Intestinal Parasites ◽  
Tap Water ◽  
Staining Technique ◽  
High Rate ◽  
Contamination Rate ◽  
Isolation And Identification ◽  
Filtration System

A total of 60 samples of drinking water filtrated by Reverser 0smosis Filtration System from April to October 2012, from different houses in Baghdad – Al Resafa, so as to identify the eggs and cysts of protozoa. Two methods applied direct smear and staining technique with zeal nelson stain, which appeared Tape warm eggs, Ascaris lumbrecoides eggs and oocyst of Cryptospordium sp. This study revealed that total contamination rate with intestinal parasites in tap water were 96.6% this high rate, refers to filtrate tap water by reverse osmosis system was useful to prevent or reduce the contamination of drinking water, in order to reduce risks to public health; So recommended to apply this method at water purification stations. Distribution of Cryptosporidium sp. as study months of year appeared that Spring (April& May ) were recorded the highest rate of contamination in filtrate tap water samples, that due to potential temperatures degrees for this protozoa as(20-30cº).When the drinking water was better as environmental maintained decreased the infection with these parasites.

In vitro effect of an aqueous extract of Artocarpus lakoocha on the intestinal parasites in cattle

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
N Saowakon ◽  
P Chaichanasak ◽  
C Wanichanon ◽  
V Reutrakul ◽  
P Sobhon
Keyword(s):  
Aqueous Extract ◽  
Intestinal Parasites ◽  
Vitro Effect

Isolation and identification of chemical constituents from the roots of Peucedanum praeruptorum

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
YJ Lee ◽  
J Kim ◽  
J Lee ◽  
ES Cho ◽  
OS Bang
Keyword(s):  
Chemical Constituents ◽  
Isolation And Identification

Isolation and identification of neuroprotective compounds from Machilus thunbergii

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
CJ Ma ◽  
KY Lee ◽  
HK Yang ◽  
JS Yoon ◽  
YC Kim ◽  
...  
Keyword(s):  
Isolation And Identification ◽  
Machilus Thunbergii

Isolation of post operative hospital acquired infections in a private hospital of Bangladesh and identification of clonal relatedness of E. coli by using Pulse filed gel electrophoresis

2012 ◽  
Vol 6 (2) ◽  
pp. 7-10
Author(s):  
Mohammad Murshed ◽  
Sabeena Shahnaz ◽  
Md. Abdul Malek
Keyword(s):  
Private Hospital ◽  
Isolation And Identification ◽  
College Hospital ◽  
Hospital Acquired Infection ◽  
E Coli ◽  
Hospital Acquired Infections ◽  
Medical College ◽  
Clonal Relatedness ◽  
Hospital Acquired ◽  
Dressing Materials

Isolation and identification of post operative hospital acquired infection was carried out from July 2008 to December 2008 in Holy Family Red Crescent Medical College Hospital (private hospital). The major pathogen of wound infection was E. coli. A total; of 120 samples were collected from the surrounding environment of post operative room like floor, bed sheets, instruments, dressing materials, catheter, nasogastric and endotracheal tube. E. coli (40%) was the predominant organism followed by S. aureus (24%). DNA fingerprinting analysis using pulsed field gel electreopheresis of XbaI restriction digested genomic DNA showed that clonal relatedness between the two clinical nd environmental isolates were 100%.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19369 Bangladesh J Med Microbiol 2012; 06(02): 7-10

Investigation of duck plague virus in hoar areas of Bangladesh

2020 ◽  
Vol 26 (1-2) ◽  
pp. 73-78
Author(s):  
A Hossen ◽  
MH Rahman ◽  
MZ Ali ◽  
MA Yousuf ◽  
MZ Hassan ◽  
...  
Keyword(s):  
Cytopathic Effect ◽  
Clinical Sample ◽  
Chorioallantoic Membrane ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Duck Plague Virus ◽  
Pcr Method ◽  
Polymerase Chain ◽  
The Individual ◽  
Free Ranging

Duck plague (DP) is the most important infectious disease of geese, ducks and free-ranging water birds. The present study was conducted to determine the prevalence of duck plague virus followed by isolation and identification. For these purposes, a total of 155 cloacal swabs samples were collected randomly from duck of different haor areas of Bangladesh including 45 (41 surveillance and 4 clinical) samples from Netrokona; 42 (40 surveillance and 2 clinical) samples from Kishoregonj; 30 samples from Brahmanbaria and 38 samples from Sunamganj. The samples were processed and pooled (1:5 ratio) for initial screening of target polymerase gene of duck plague virus by polymerase chain reaction (PCR) method. All the samples of a positive pool were then tested individually for identifying the individual positive samples. The result showed that out of 155 samples, 41 (26.45%) were found positive in which 17 were from Netrokona, where 15 (36.58%) were from surveillance samples and 2 (50%) were from clinical sample; 16 were from Kishoregonj, where 14 (35%) were from surveillance samples and 2 (100%) were from clinical sample; 2 (6.6%) were from Brahmanbaria and 5 (13.15%) were from Sunamganj. These positive samples were inoculated into 9-10 days embryonated duck eggs (EDE) through chorioallantoic membrane (CAM) route for the isolation of virus. The EDE died earlier was also chilled, and in a similar way, the CAMs were collected and again performed PCR for id entification of virus. Out of 41 PCR positive samples, 26 samples were isolated and reconfirmed by PCR. Subsequently, DPV was isolated in primary duck embryo fibroblasts cell culture and confirmed by observing cytopathic effect (CPE). Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 73-78

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