scholarly journals PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY POTENTIAL EVALUATIONS OF METHANOLIC EXTRACTS OF COCOS NUCIFERA (L.) LEAVES

2019 ◽  
Author(s):  
Md. Saeedul Alam Nizami ◽  
Kazi Mobarak Hossain ◽  
Md. Shahidul Islam
Keyword(s):  
Plant Extracts ◽  
Methanolic Extract ◽  
Cocos Nucifera ◽  
Antioxidant Effect ◽  
Pharmacological Activities ◽  
Ic50 Values ◽  
Anti Inflammatory ◽  
Dpph Scavenging ◽  
Cocos Nucifera L ◽  
Dpph Scavenging Assay

Cocos nucifera (L.) (Arecaceae) is commonly called the ‘‘coconut tree’’ and is the most naturally widespread fruit plant on Earth. Throughout history, humans have used medicinal plants therapeutically, and minerals, plants, and animals have traditionally been the main sources of drugs. The objective in the present study was to screen the phytochemical profile and pharmacological activities of methanolic extract of coconut leaves. To investigate pharmacological activities DPPH scavenging assay and HRBC membrane stabilization methods were performed for antioxidant and anti-inflammatory potential respectively. The phytochemical analysis of methanolic extract of coconut leaves showed that they contained significant presence of flavonoids, phenols , saponins, terpenoids and triterpenes. Alkaloids, glycosides and tannins are also moderately present. Quantitative evaluations show significant presence of phenols which was more than tannin content. The pharmacological studies revealed that the plant extracts may have significant antioxidant effect which is probably mediated by inhibition of DPPH free radical. The IC50 values by DPPH scavenging assay observed for standard and leaves were 97.29µg/ml and 486.78µg/ml respectively. Thus, this plant extracts have significant antioxidant effect. It also had moderate anti-inflammatory activity. The IC50 values for anti-inflammatory activity by standard and coconut leaves were 21.46 µg/ml and 831.21 µg/ml respectively. These findings suggest that Cocos nucifera (L.) may be a possible source for the development of a newanti-inflammatory drug.

scholarly journals Demonstration of Phytochemicals and In-vitro Antioxidant and Anti-inflammatory activity by methanolic extract of Elettaria cardamomum

2020 ◽  
Vol 3 (3) ◽  
pp. 01-04
Author(s):  
Md. Shahidul Islam
Keyword(s):  
Methanolic Extract ◽  
Research Work ◽  
Inflammatory Activity ◽  
Pharmacological Activities ◽  
Elettaria Cardamomum ◽  
Ic50 Values ◽  
Phytochemical Profile ◽  
Anti Inflammatory ◽  
Dpph Scavenging

The existing research study attempts to untie novel avenues for development of the medicinal exercises of Elettaria cardamomum, fashionable known as the “Queen of Spices” and locally recognized as “elaichi”. Its seeds are utilized as abortifacient, acrid, alexiteric, aromatic, sweet, cardiac tonic, cooling, carminative, digestive, diuretic, expectorant, stimulant and also tonic beneficial in the asthma, haemorrhoids, bronchitis, strangury, renal in addition to vesical calculi, anorexia, halitosis, gastropathy dyspepsia as well as burning sensation. The prime goal of this research work is to evaluate antioxidant as well as anti-inflammatory properties of the traditional Bangladeshi medicinal extracts in addition to examine these activities. The aim in present work was to screen the phytochemical profile as well as pharmacological activities of the methanolic extract of this plant’s leaves. To explore pharmacological actions DPPH scavenging test and the HRBC membrane stabilization techniques were done for the antioxidant and also anti-inflammatory test respectively. The pharmacological works revealed that plant extracts might have noteworthy antioxidant effect which is possibly mediated by the inhibition of DPPH free radical which is accountable for oxidation. The IC50 values by the DPPH scavenging test observed for the standard and the leaves were 106.38µg/ml & 594.47µg/ml respectively. There is also moderate anti-inflammatory activity. The IC50 values for anti-inflammatory activity by standard & leaves were 35.04µg/ml and 944.0 µg/ml respectively.

scholarly journals Components from the Leaves and Twigs of Mangrove Lumnitzera racemosa with Anti-Angiogenic and Anti-Inflammatory Effects

Marine Drugs ◽  
2018 ◽  
Vol 16 (11) ◽  
pp. 404 ◽  
Author(s):  
Szu-Yin Yu ◽  
Shih-Wei Wang ◽  
Tsong-Long Hwang ◽  
Bai-Luh Wei ◽  
Chien-Jung Su ◽  
...  
Keyword(s):  
Spectroscopic Data ◽  
Methanolic Extract ◽  
Data Interpretation ◽  
Tube Formation ◽  
Biosynthesis Pathway ◽  
Angiogenic Effect ◽  
Ic50 Values ◽  
Anti Inflammatory ◽  
Lumnitzera Racemosa

One new neolignan, racelactone A (1), together with seven known compounds (2−8) were isolated from the methanolic extract of the leaves and twigs of Lumnitzera racemosa. The structure of racelactone A (1) was determined on the basis of the mass and NMR spectroscopic data interpretation. With respect to bioactivity, compound 1 displayed an anti-angiogenic effect by suppressing tube formation. Furthermore, compounds 1, 4, and 5 showed significant anti-inflammatory effects with IC50 values of 4.95 ± 0.89, 1.95 ± 0.40, and 2.57 ± 0.23 μM, respectively. The plausible biosynthesis pathway of racelactone A (1) was proposed.

scholarly journals Evaluation of the Antioxidant and Antiradical Properties of Some Phyto and Mammalian Lignans

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7099
Author(s):  
Leyla Polat Kose ◽  
İlhami Gulcin
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Nordihydroguaiaretic Acid ◽  
Butylated Hydroxytoluene ◽  
Frap Assay ◽  
Antiradical Properties ◽  
Reducing Ability ◽  
Ic50 Values ◽  
Dpph Scavenging ◽  
Dpph Scavenging Assay

In this study, the antioxidant and antiradical properties of some phyto lignans (nordihydroguaiaretic acid, secoisolariciresinol, secoisolariciresinol diglycoside, and α-(-)-conidendrin) and mammalian lignans (enterodiol and enterolactone) were examined by different antioxidant assays. For this purpose, radical scavenging activities of phyto and mammalian lignans were realized by 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical (ABTS•+) scavenging assay and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging assay. Additionally, the reducing ability of phyto and mammalian lignans were evaluated by cupric ions (Cu2+) reducing (CUPRAC) ability, and ferric ions (Fe3+) and [Fe3+-(TPTZ)2]3+ complex reducing (FRAP) abilities. Also, half maximal inhibitory concentration (IC50) values were determined and reported for DPPH• and ABTS•+ scavenging influences of all of the lignan molecules. The absorbances of the lignans were found in the range of 0.150–2.320 for Fe3+ reducing, in the range of 0.040–2.090 for Cu2+ reducing, and in the range of 0.360–1.810 for the FRAP assay. On the other hand, the IC50 values of phyto and mammalian lignans were determined in the ranges of 6.601–932.167 µg/mL for DPPH• scavenging and 13.007–27.829 µg/mL for ABTS•+ scavenging. In all of the used bioanalytical methods, phyto lignans, as secondary metabolites in plants, demonstrated considerably higher antioxidant activity compared to that of mammalian lignans. In addition, it was observed that enterodiol and enterolactone exhibited relatively weaker antioxidant activities when compared to phyto lignans or standard antioxidants, including butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), Trolox, and α-tocopherol.

scholarly journals Characterization of the antinociceptive and anti-inflammatory activities from Cocos nucifera L. (Palmae)

2009 ◽  
Vol 122 (3) ◽  
pp. 541-546 ◽  
Author(s):  
Sebastian Rinaldi ◽  
Davi O. Silva ◽  
Fabiana Bello ◽  
Celuta S. Alviano ◽  
Daniela S. Alviano ◽  
...  
Keyword(s):  
Cocos Nucifera ◽  
Anti Inflammatory ◽  
Cocos Nucifera L

scholarly journals Comparative HPTLC Analysis of Leaves of Allium cepa L., Ficus carica L. and Ziziphus mauritiana L. with Standard Quercetin

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
Une H. D. ◽  
Khan D. ◽  
Mohammed M.
Keyword(s):  
Quantitative Analysis ◽  
Allium Cepa ◽  
Plant Extracts ◽  
Calibration Curve ◽  
Methanolic Extract ◽  
Solvent System ◽  
Ficus Carica ◽  
Ziziphus Mauritiana ◽  
Pharmacological Activities ◽  
Allium Cepa L

Flavonols aglycones are well established in plant extracts with omnipresence of common flavonols like kaempferol, quercetin and myricetin. Quercetin has been reported for various pharmacological activities with major ones in the field of inflammation, cancer, arthritis, diabetes and its complications. Leaves of plants selected for this study viz. Allium cepa L., Ficus carica L. and Ziziphus mauritiana L. reported to have antioxidant, anticancer, hypoglycemic activities. It is hypothetised thisactivities are due to flavonoids present in these plants. Hence the ethylacetate fraction of methanolic extract of these leaves was obtained as it provides flavanoid rich fraction and the samples were prepared and HPTLC analysis was performed against standard quercetin and their quantitative analysis was performed, the solvent system toluene: ethyl acetate: formic acid (9: 1: 0.5) was selected. It was observed that these plants shows presence of quercetin where A. cepa has highest concentration with F. carica and Z. mauritiana having quercetin in declining order. The calibration curve was linear in the range of 0.1 to 0.7 μg for quercetin. Further a correlation coefficient of 0.999 indicates good linearity between concentration and area. Thus, it was proved that the plants used in the study has presence of quercetin which is possibly the reason having the pharmacological uses.

scholarly journals In vitro and in vivo anti-inflammatory activity of Dillenia pentagyna Roxb. bark, a folklore medicine of Mizoram

2017 ◽  
Vol 17 (2) ◽  
pp. 69-77
Author(s):  
Zothanpuia ◽  
Bibhuti K. Kakoti
Keyword(s):  
Methanolic Extract ◽  
Inflammatory Activity ◽  
Endangered Plants ◽  
Pharmacological Activities ◽  
North East India ◽  
Rich Variety ◽  
North East ◽  
Anti Inflammatory

Natural products have played an exceptionally important role in health care and prevention of diseases for thousands of years and are one of the greatest sources of medicine. Mizoram is a home to a rich variety of flora and fauna however; very less systematic survey and documentation were carried out to date. The plant diversity of Mizoram is not adequately studied as compared to other states of north east India. Dillenia pentagyna Roxb., known as kaihzawl in Mizo and dog teak in English, is one of the endangered plants that is yet to be explore. The Mizo communities use the decoction of D. pentagyna bark to treat different stomach ailments, inflammation and diabetes. In this perspective, the methanolic extract of the bark of D. pentagyna was evaluated for its anti-inflammatory properties. The extract showed the presence of flavanoids, triterpenoids, steroids, phenolics, saponins, fixed oils and others exerting varied pharmacological activities. The methanolic extract of this plant was found to contain active constituents such as betulinic acid and β-sitosterol which are found to actively interact with the inflammatory mediators and suppressed the inflammation when evaluated for its in vitro and in vivo anti-inflammatory activity. The present study suggests that the methanolic extract of D. pentagyna bark showed significant anti-inflammatory activity. This study also support the folkloric used of the plant in diseases related to inflammatory condition.

scholarly journals Steric Effect of Antioxidant Diels-Alder-Type Adducts: A Comparison of Sanggenon C with Sanggenon D

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2610 ◽  
Author(s):  
Xican Li ◽  
Zhenxing Ren ◽  
Zimei Wu ◽  
Zhen Fu ◽  
Hong Xie ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Steric Effect ◽  
Bathochromic Shift ◽  
Radical Scavenging ◽  
Diels Alder ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Ic50 Values ◽  
Dpph Scavenging ◽  
Dpph Scavenging Assay

Sanggenons C and D are two Diels-Alder-type adducts from Chinese crude drug Sang-bai-pi. Structurally, both sanggenons construct stereoisomers. In the study, they were comparatively determined using four antioxidant assays, including ferric ion reducing antioxidant power (FRAP) assay, Cu2+-reducing assay, 1,1-diphenyl-2-picryl-hydrazl (DPPH•)-scavenging assay, and 2,2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid radical (ABTS•+)-scavenging assay. Their Fe2+-binding reactions were explored using UV-Vis spectra. Finally, their cytoprotective effects were evaluated using flow cytometry. In electron transfer (ET)-based FRAP and Cu2+-reducing assays, sanggenon D was found to have lower IC50 values than sanggenon C; however, in multi-pathway-based DPPH•-scavenging and ABTS•+-scavenging assays, sanggenon C possessed lower IC50 values than sanggenon D. UV-Vis spectra suggested that sanggenon C generated a bathochromic-shift (286 nm → 302 nm) and displayed stronger UV absorption than sanggenon D. In flow cytometry, sanggenon C and sanggenon D, respectively, exhibited 31.1% and 42.0% early apoptosis-percentages towards oxidative-stressed mesenchymal stem cells (MSCs). In conclusion, both sanggenons may undergo multiple pathways (e.g., ET and Fe2+-binding) to protect MSCs against oxidative stress. In the mere ET aspect, sanggenon D possesses a higher level than sanggenon C, while in multi-pathway-based radical-scavenging, Fe2+-binding, and cytoprotection aspects, sanggenon C is more active than sanggenon D. These discrepancies can conclusively be attributed to the steric effect.

Virgin coconut oil supplementation ameliorates cyclophosphamide-induced systemic toxicity in mice

2015 ◽  
Vol 35 (2) ◽  
pp. 205-212 ◽  
Author(s):  
SS Nair ◽  
JJ Manalil ◽  
SK Ramavarma ◽  
IM Suseela ◽  
A Thekkepatt ◽  
...  
Keyword(s):  
Protective Efficacy ◽  
Cocos Nucifera ◽  
Experimental Studies ◽  
Coconut Oil ◽  
Thiobarbituric Acid ◽  
Similar Efficacy ◽  
Systemic Toxicity ◽  
Virgin Coconut Oil ◽  
Anti Inflammatory ◽  
Cocos Nucifera L

Virgin coconut oil (VCO) is an unrefined kernal oil, prepared from Cocos nucifera L., having substantial nutritional and medicinal value. Experimental studies have suggested its antioxidant, anti-inflammatory, immunostimulatory and hypolipidemic effects. The present study assesses its effect on formalin-induced chronic inflammation and cyclophosphamide (CTX)-induced systemic toxicity in murine models. Oral administration of VCO effectively reduced formalin-induced paw oedema in mice with more or less similar efficacy as that of diclofenac. The CTX-induced hike in blood urea, creatinine, thiobarbituric acid reactive substances (TBARS) and liver marker enzymes in mice was marginally decreased by VCO (8 g/kg body weight) ingestion orally. The liver and kidney catalase, superoxide dismutase and glutathione peroxidase activities, together with cellular glutathione and TBARS levels, were found to be improved in these animals. Overall the study reveals the protective efficacy of VCO against secondary toxicity induced by CTX possibly through its antioxidant and anti-inflammatory properties.

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