scholarly journals Activity of Muntingia calabura Leaves Ethanolic Extract on Glucose and Insulin Blood Levels in Streptozotocin-induced Rat

1970 ◽  
Vol 7 (4) ◽  
pp. 8-11
Author(s):  
Suci Syahara ◽  
Urip Harahap ◽  
Tri Widyawati
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Blood Levels ◽  
Control Group Design ◽  
Insulin Levels ◽  
Blood Insulin ◽  
Glucose Levels ◽  
Muntingia Calabura

Objectives: The research  aims to determine the effect of Muntingia calabura leaves extract on improving diabetes conditions, by analyzing blood glucose and insulin levels of male Rattus novergicus which is given high fat diet and induce streptozotocin 30 mg/kg BB with a two-week interval. Design: This research was a purely experimental with pretest and post test control group design. This research was divides into 6 groups. Three treatment groups were treated by Muntingia calabura leaves extract with the doses of 125, 250, and 500 mg/kg. Other groups are negative control (CMC-Na), positive control (glibenklamid) and normal control. Interventions: The variables specified in this study are variations in the concentration of extract used. variations in extract concentration are expected to affect the results of glucose concentration and blood insulin in rats. Main outcome measure: The main parameters measured in this study were rat blood glucose and insulin levels. Results: The result of this research indicate that the ethanol extract of Muntingia calabura leaves able to reduce blood glucose levels effectively at dose 500 mg/kg (97, 17 mg/dL) so that insulin levels increase well (0,839 µg/mL). Conclusion: Muntingia calabura leaves ethanol extract can reduce glucose levels and increase blood insulin levels in rats.  

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals UJI AKTIVITAS ANTIDIABETES EKSTRAK ETANOL BUAH CABAI MERAH ( Capsicum annuum L. ) TERHADAP MENCIT PUTIH JANTAN

2019 ◽  
Vol 9 (1) ◽  
pp. 86 ◽  
Author(s):  
Zulkarni Zulkarni
Keyword(s):  
Blood Glucose ◽  
Capsicum Annuum ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Capsicum Annuum L ◽  
Blood Glucose Levels ◽  
Significant Difference

This Research was conducted to determine the effect of ethanol extract from red chilli (Capsicum annuum L)in lowering blood glucose levels of hyperglycemic male white mices. This study used 30 malewhitemices and divided into 6 groups: negative control group, the positive control group, the treatmentgroup withdosage of 200 mg / kgweight, 400 mg / kgwieght, 600 mg / kg weight and a comparison group with glibenclamide with dosage of 5 mg / kgweightadministered orally for 21 days. The level of fasting blood glucose was checked 6 days after dexamethasone induced, and after the ethanol extract of red chilies on day 7th, 14th, and 21st. The data was analyzed statistically with one –way and two-way Anova by usingSPSS16 program and proceed with the test Duncan to look the significant difference between treatments. The results showed that the ethanol extract from red chilies with a dosage of 200mg / kgweight, 400mg / kgweight, 600 mg / kgweight showed the effect in lowering blood sugar levels in male white mices hyperglycemia significantly (p <0.05). The duration ethanol extract of red chili affected blood glucose levels. The most effective duration in lowering blood glucose levels is the administration of a preparation within 21 days.

The Effect of Ethanol Extract of Guava Leaf on Hypercholesterolemia-Diabetes of White Rats Male Induced by High Fat Feed and Streptozotocin

2021 ◽  
Vol 7 (2) ◽  
pp. 177-187
Author(s):  
Joni Tandi ◽  
Franciska Diana Pratiwi ◽  
Surisna Surisna ◽  
Niluh Puspita Dewi ◽  
Magfirah Magfirah
Keyword(s):  
Blood Glucose ◽  
Effective Dose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Group I ◽  
Guava Leaf

This study aims to determine the effect of guava leaf extract (Psidiium guajava L) on hypercholesterolemia-diabetes and the effective dose for reducing cholesterol and blood glucose levels. This study is a laboratory experimental study using 30 rats divided into 6 test animals. the treatment group, each group consisted of 5 rats. Group I was normal control, group II negative control was given Na-CMC suspension, group III positive control was given simvastatin group IV dose 150 mg / kg BW, group V dose 250 mg / kg BW and group VI dose 350 mg / kg BW. The results showed that the ethanol extract of guava leaves had an effect on reducing blood glucose levels of male white rats with an effective dose of 250mg / kg BW with an average reduction of 119 mg/dL and effectively reduce cholesterol levels at a dose of 150 mg /kg BW with an average reduction of 28,33 mg/dL.

scholarly journals Evaluation the Effect of Vernonia amygdalina. Del Leaves Ethanol Extract on Blood Sugar and its Synergism with Glibenclamide in Rats

2020 ◽  
Vol 13 (1) ◽  
pp. 26-34
Author(s):  
Igwe K ◽  
Ikpeazu O ◽  
Otuokere I
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Treatment Groups ◽  
Glucose Levels

Antidiabetic activity of Vernonia amygdalina and its possible synergism with glibenclamide was checked. Forty eight rats were used for the research, for hypoglycermic study of V. amygdalina alone, they were grouped into five of six rats each. Group 1 was the negative control and was administered distilled water orally. Groups 2, 3, and 4 were the treatment groups which received 100, 200 and 300 mg/kg body weight of the V. amygdalina extract respectively orally by intubation. Group 5 was the positive control group which received a known antidiabetic drug, glibenclamide. Diabetes was induced with alloxan. For the synergism study, another 18 rats grouped into 3 of six rats each was used. Both groups of glibenclamide only and glibenclamide plus V. amygdalina extract were dosed for 14 days orally by intubation, thereafter were sacrificed and blood collected from heart for analysis. There were 5 replicates grouped by weight throughout the study and both single and synergistic studies had the same controls. Effect of V. amygdalina extract was checked on blood glucose and its possible synergism with glibenclamide. All results in treatment groups were compared with the normal control at statistical confidence of p<0.05. Result shows that V. amygdalina extract reduced blood glucose level in the test groups as dose of extract increased. Combination of V. amygdalina with glibenclamide demonstrated further deduction in blood glucose levels in the treatment rats groups. Therefore addition of V. amygdalina into glibenclamide increased efficacy in the diabetic rats. The interaction between V. amygdalina and glibenclamide in this work was additive and therefore synergistic.

scholarly journals NANOEMULSION OF OKRA FRUIT EXTRACT AS ANTIDIABETIC TREATMENT

2020 ◽  
pp. 138-142
Author(s):  
RATNA DJAMIL ◽  
SARAH ZAIDAN ◽  
DENI RAHMAT ◽  
DIAH KARTIKA PRATAMI ◽  
FELIX HAKIM
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Glucose Levels ◽  
Antidiabetic Treatment ◽  
Blood Glucose Levels ◽  
Average Blood Glucose

Objective: Okra (Abelmoschus esculentus (L.) Moench) has potential antidiabetic activity. This study created a nanoemulsion of okra extract (NOE) and examined its activity on alloxan-induced diabetes mellitus in mice. Methods: Okra was macerated with 70% ethanol and dried in a rotary evaporator into the crude extract. The extract was encapsulated in a solution of glyceryl caprylate, propylene glycol, and glycerine to form a nanoemulsion. To determine the antihyperglycaemic effect of okra extract, 35 male mice (Mus musculus L.) were divided into seven groups: a non-diabetic normal control group and six diabetic mice groups (untreated negative control, glibenclamide-treated positive control, and four treatments with okra ethanol extract (OEE) at 200 and 400 mg/kg BW and NOE at 200 and 400 mg/kg BW). Results: The group treated with NOE at 400 mg/kg BW (NOE400) had the lowest average blood glucose level of 93.4 mg/dL among hyperglycaemic mice. The decrease in blood glucose levels in NOE400 (52.05%) was significantly different from those in the positive control (42.63%) and OEE treatments (39.32%). The nanoemulsion used in this study fulfills quality requirements, with a mean particle size of 134.7 nm, a polydispersity index of 0.512, and a zeta value of −26.72 mV. Conclusion: NOE reduced blood glucose levels in alloxan-induced hyperglycaemic mice better than OEE. Nanoemulsion can improve the antidiabetic activity of okra extract by increasing penetration of active compounds into interstitial space so that their delivery and bioavailability are higher.

scholarly journals Pengaruh Pemberian Infusa Daun Kersen (Muntingia calabura L.) terhadap Kadar Glukosa Darah Mencit Putih (Mus musculus) yang Diberi Beban Glukosa

2019 ◽  
Vol 8 (2) ◽  
pp. 60-66
Author(s):  
Mira Febrina
Keyword(s):  
Blood Glucose ◽  
Mus Musculus ◽  
Animal Experiments ◽  
Negative Control ◽  
Control Group ◽  
Oral Glucose ◽  
Glucose Load ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Muntingia Calabura

A study on the effect of cherry leaf infusion (Muntingia calabura L.) on glucose levels of male white mice (Mus musculus) given glucose load. This study aims to see the effect of cherry leaf infusion (Muntingia calabura L.) to blood glucose levels of male white mice (Mus musculus) given glucose load as much as 0.65ml / 20gBB. This research used Oral Glucose Tolerance Test (TTGO) method. Animal experiments were divided into 6 groups. The negative control group was given only aquadest, the positive group was given metformin with the dosage of 1.3mg / 20gBB, the comparison group was given 0.65ml / 20gBB glucose solution, the treatment group was given cesen leaves infusion (Muntingia calabura L.) with concentration 5, 10 and 20 %. After each test animal was given 30 minutes treatment then given glucose 0.65ml / 20gBB orally. Then measured blood glucose levels of mice at minute 30-180. Based on the results of testing the infusion of kersen leaf (Muntingia calabura L.) showed that the concentrations of 5, 10 and 20% had decreased levels of white mice blood glucose (Mus musculus) with normal control. However, the results of the two-way ANOVA assay continued with the Post Hoc Tukey test showed that the concentration of 20% differed significantly with the concentrations of 5 and 10%. And the results obtained also indicate that the duration of administration may affect the decrease in blood glucose levels of mice (p <0.05).

scholarly journals The Effectiveness of Vitamin D Addition to Decreasing Blood Glucose Levels of Hyperglycemic Mice Model with Glimepirid Therapy

2020 ◽  
Vol 6 (2) ◽  
pp. 71
Author(s):  
Tsintani Nur Aristiana ◽  
Cholis Abrori ◽  
Muhammad Ali Shodikin
Keyword(s):  
Diabetes Mellitus ◽  
Vitamin D ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Negative Control ◽  
Control Group ◽  
Mice Model ◽  
Control Group Design ◽  
Glucose Levels ◽  
Blood Glucose Levels

Abstract Low 25-hydrocyvitamin D (25(OH)D3) levels are usually found in patients with type 1 and type 2 diabetes mellitus. This study aims to compare the effectiveness of fasting blood glucose levels in hyperglycemic mice model with glimepirid single therapy, hyperglycemic mice model with vitamin D, and mice model of hyperglycemic with glimepirid therapy plus vitamin D. This study used the true experimental research design with the randomized posttest only control group design. There were 25 male mice (Mus musculus) strain Balb/C with 20-30 g body weight and 2-3 months old which were grouped into 5 groups. The negative control group (K1) was injected with placebo and the positive control group (K2) was injected with STZ 150 mg/kgBW i.p. Groups P1, P2, and P3 were given STZ injection of 150 mg/kgBW i.p and given glimepirid 0.26 mg/kgBW; vitamin D 6,5 ml/kgBW; glimepirid 0,26 mg/kgBW with vitamin D supplementation 6,5 ml/kgBW. The results of the Tukey post hoc tests obtained differences in the mean of delta KGD between groups P1 and K2 = 122,55; in group P2 with K2 = 81,60; and in group P3 with K2 = 74,40. From these data, it can be concluded that in the group given glimepirid plus vitamin D there was no additional effectiveness in decreasing fasting blood glucose levels of hyperglycemic mice model compared to the group given only glimepirid therapy and the group given vitamin D alone. Keywords: diabetes mellitus, vitamin D, glimepiride, streptozotocin.

PENGARUH PEMBERIAN FRAKSI ETILASETAT KULIT UBI JALAR UNGU TERHADAP KADAR MALONDIALDEHID (MDA) SERUM MENCIT PUTIH JANTAN HIPERGLIKEMIA

2018 ◽  
Vol 8 (2) ◽  
pp. 144
Author(s):  
Ria Afrianti
Keyword(s):  
Blood Glucose ◽  
Statistical Analysis ◽  
Sweet Potato ◽  
Ethyl Acetate Fraction ◽  
Serum Levels ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Purple Sweet Potato

This study aims to determine the effect giving of ethylacetate fraction of leather  purple sweet potato (Ipomoea batatas (L.) Lam, on levels of malondialdehyde (MDA) serum in mice hyperglicemia were induced with streptozocin dose of 50 mg/kgBW. Mice were divided into 5 groups, each group consisting of 3 tails, group I is a negative control, group II is a positive control, group III,IV and V is given ethylacetate fraction a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW. Ethyl Acetate Fraction leather purple sweet potato given orally for 15 days after the animal is declared hyperglicemia and measurement of blood glucose levels on 5, 10, and 15 day after giving test preparation in animal experiments. On the 16 day throughout the mice were taken serum levels measured malondialdehid. The statistical analysis results showed that giving of ethyl acetate fraction of leather purple sweet potato at a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW can lower blood glucose levels in mice hyperglycemia significantly (p<0.05). Malondialdehid levels on average in each group is 1.35 nmol/ml, 3.00 nmol/ml, 2.72 nmol/ml, 2.20 nmol/ml and 2.61 nmol/ml, the results of statistical analysis showed a decrease in melondialdehid serum levels were significantly (p<0.05), where a dose of 300 mg/kgBW is an effective dose for lowering blood glucose levels followed by decreased levels of malondialdehid which give effect approaching negative control.

scholarly journals UJI ANTIDIABETIK EKSTRAK ETANOL BUNGA PEPAYA (Carica papaya L.) TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 160
Author(s):  
Adinda Fransisca Pongoh ◽  
Edwin De Queljoe ◽  
Henki Rotinsulu
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Carica Papaya ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Control Group ◽  
Homogeneity Test ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

ABSTRACT This study aims to determine the antidiabetic activity of papaya flower ethanol extract (Carica papaya L.) against male white rats (Rattus norvegicus) induced by alloxan. This research is experimental. Fifteen rats were divided into 5 treatment groups, each group consisted of 3 rats. The first group was the negative control group given Aquades, the second group was positive control given Glibenklamid, the three groups were 200 mg dose variation groups, four groups were 400 mg dose variations, and the five groups were 800 mg dose variations. Previously, rats were examined fasting blood glucose levels, then mice were induced by an alloxan dose of 120 mg / kgBW intraperitoneally. On the 3rd day blood glucose levels were examined and then treated according to groups for 7 days, measurement of blood glucose levels after the treatment was carried out once every 3 days namely day 3, day 7, and day 10. Data obtained were then analyzed statistically using SPSS , including normality test (Shapiro-Wilk), homogeneity test (Levene), ANOVA test (One way). The results of this study indicate that Papaya Flower (Carica papaya L.) Ethanol Extract can provide the best blood glucose level reduction effect at a dose of 800 mg. Keywords : Antidiabetic, Papaya Flower Extract (Carica papaya L.), Diabettes Mellitus, Male White Rat (Rattus norvegicus), Aloxan.  ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas Antidiabetik Ekstrak Etanol Bunga Pepaya (Carica papaya L.) Terhadap Tikus Putih Jantan (Rattus norvegicus) Yang Diinduksi Aloksan. Penelitian ini bersifat eksperimental. Sebanyak 15 ekor tikus dibagi ke dalam 5 kelompok perlakuan masing- masing kelompok terdiri dari 3 ekor tikus. Kelompok pertama kelompok Kontrol negatif yang diberikan Aquades, Kelompok kedua Kontrol Positif yang diberikan Glibenklamid, kelompok tiga kelompok variasi dosis 200 mg, kelompok empat kelompok variasi dosis 400 mg, dan kelompok lima kelompok variasi dosis 800 mg. Sebelumnya tikus dilakukan pemeriksaan kadar glukosa darah puasa, selanjutnya tikus diinduksi Aloksan dosis 120 mg/kgBB secara intraperitoneal. Pada hari ke 3 diperiksa kadar Glukosa darah kemudian diberikan perlakuan sesuai kelompok selama 7 hari, pengukuran kadar glukosa darah setelah perlakuan dilakukan 3 hari sekali yaitu hari ke 3, hari ke 7, dan hari ke 10. Data yang diperoleh kemudian dianalisis secara statistik menggunakan SPSS, meliputi uji normalitas (Shapiro-Wilk), uji homogenitas (Levene), uji ANOVA (One way). Hasil penelitian ini menunjukkan bahwa Ekstrak Etanol Bunga Pepaya (Carica papaya L.)  dapat memberikan efek penurunan kadar glukosa darah yang paling baik yaitu pada dosis 800 mg. Kata kunci : Antidiabetik, Ekstrak Bunga pepaya (Carica papaya L.), Diabettes Mellitus, TikusPutih Jantan (Rattus norvegicus), Aloksan.

scholarly journals Effectiveness of Antidiarrheal Unripe Wooden Banana (Musa paradisiaca L.) in Male Balb-C/Mice Induced with Escherichia coli

2020 ◽  
Vol 56 (3) ◽  
pp. 208
Author(s):  
Arista Wahyu Ningsih ◽  
Maftuchah Rochmanti ◽  
Achmad Basori
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Negative Control ◽  
Morbidity Rate ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Musa Paradisiaca ◽  
Fecal Weight ◽  
Antidiarrheal Activity

The survey results in Indonesia in 2017 showed that the diarrhea morbidity rate for all age groups was 6.897.463. In Indonesia, unripe wooden banana has been used empirically as an antidiarrheal by the people in Senduro village, Lumajang, East Java. The study aimed to prove the antidiarrheal effect of ethanol extract of unripe wooden banana (Musa paradisiaca L.) in male Balb-C/mice induced by Escherichia coli bacteria. This study was a laboratory experimental study with post-test only control group design that used 40 mice divided into 8 groups, namely 1 group without treatment (normal mice) and 7 groups induced by Escherichia coli (1 negative control group given CMC-Na, 3 groups positive controls were given Loperamid HCL 0,5 mg/kgBW, 1 mg/kgBW and 2 mg/kgBW and 3 treatment groups were given extracts 100 mg/kgBW, 200 mg/kgBW and 400 mg/kgBW). Observation of animals in this study was carried out for 4 hours. Data were analyzed of frequency of diarrhea, fecal weight and fecal consistency used Kruskal Wallis and the results showed significant differences between treatment groups (p <0.05). From the results of the Mann-Whitney test, the ethanol extract of unripe wooden banana with a dose of 100 mg/kgBW was not significantly different (p> 0.05) with the control group of Loperamid HCL dose of 0.5 mg/kgBW. Unripe wooden banana had been shown to have antidiarrheal activity at an extract dose of 100 mg/kg BW in terms of the parameters of fecal consistency, frequency of diarrhea, and fecal weight. The results showed that the higher the dose, the better antidiarrheal activity.

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