scholarly journals TIMING OF DETECTION OF VIRUS PARTICLES IN PALISADE AND SPONGY PARENCHYMA CELLS OF TOBACCO LEAVES INOCULATED WITH TOBACCO MOSAIC VIRUS

Uirusu ◽  
1972 ◽  
Vol 22 (1-2) ◽  
pp. 55-57
Author(s):  
Yasushi TAKAGI ◽  
Takashi KAGI ◽  
Rokuo USHIYAMA
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Spongy Parenchyma ◽  
Tobacco Leaves ◽  
Virus Particles ◽  
Palisade And Spongy Parenchyma ◽  
Parenchyma Cells

scholarly journals RELATION OF TOBACCO MOSAIC VIRUS TO THE HOST CELLS

1967 ◽  
Vol 33 (3) ◽  
pp. 665-678 ◽  
Author(s):  
Katherine Esau ◽  
James Cronshaw
Keyword(s):  
Electron Microscope ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Host Cells ◽  
Vascular Bundles ◽  
Mesophyll Cells ◽  
Virus Particles ◽  
Nicotiana Tabacum L ◽  
Parenchyma Cells ◽  
Particle Aggregates

The relation of tobacco mosaic virus (TMV) to host cells was studied in leaves of Nicotiana tabacum L. systemically infected with the virus. The typical TMV inclusions, striate or crystalline material and ameboid or X-bodies, which are discernible with the light microscope, and/or particles of virus, which are identifiable with the electron microscope, were observed in epidermal cells, mesophyll cells, parenchyma cells of the vascular bundles, differentiating and mature tracheary elements, and immature and mature sieve elements. Virus particles were observed in the nuclei and the chloroplasts of parenchyma cells as well as in the ground cytoplasm, the vacuole, and between the plasma membrane and the cell wall. The nature of the conformations of the particle aggregates in the chloroplasts was compatible with the concept that some virus particles may be assembled in these organelles. The virus particles in the nuclei appeared to be complete particles. Under the electron microscope the X-body constitutes a membraneless assemblage of endoplasmic reticulum, ribosomes, virus particles, and of virus-related material in the form of wide filaments indistinctly resolvable as bundles of tubules. Some parenchyma cells contained aggregates of discrete tubules in parallel arrangement. These groups of tubules were relatively free from components of host protoplasts.

scholarly journals THE STRUCTURE OF CELLS DURING TOBACCO MOSAIC VIRUS REPRODUCTION

1965 ◽  
Vol 25 (3) ◽  
pp. 77-97 ◽  
Author(s):  
L. Kolehmainen ◽  
H. Zech ◽  
D. von Wettstein
Keyword(s):  
Endoplasmic Reticulum ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Electron Scattering ◽  
Osmium Tetroxide ◽  
Crystal Formation ◽  
Mesophyll Cells ◽  
Virus Particles ◽  
Flexible Rods ◽  
Late Stages

The submicroscopic organization of mesophyll cells from tobacco leaves systemically infected with tobacco mosaic virus (TMV) is described. After fixation with glutaraldehyde and osmium tetroxide the arrangement of the TMV particles within the crystalline inclusions is well preserved. Only the ribonucleic acid-containing core of the virus particles is visible in the micrographs. Besides the hexagonal virus crystals, several characteristic types of "inclusion bodies" are definable in the cytoplasm: The so-called fluid crystals seem to correspond to single layers of oriented TMV particles between a network of the endoplasmic reticulum and ribosomes. Unordered groups or well oriented masses of tubes with the diameter of the TMV capsid are found in certain areas of the cytoplasm. A complicated inclusion body is characterized by an extensively branched and folded part of the endoplasmic reticulum, containing in its folds long aggregates of flexible rods. Certain parts of the cytoplasm are filled with large, strongly electron-scattering globules, probably of lipid composition. These various cytoplasmic differentiations and the different forms of presumed virus material are discussed in relation to late stages of TMV reproduction and virus crystal formation.

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