scholarly journals In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung

2021 ◽  
Author(s):  
Endah Setyaningrum ◽  
Achmad Arifiyanto ◽  
Nismah Nukmal ◽  
Titik Nur Aeny ◽  
Meishy Handerlin Putri ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Ethyl Acetate ◽  
Probit Analysis ◽  
Streptomyces Hygroscopicus ◽  
In Vitro Test ◽  
Ic50 Value ◽  
Vitro Test ◽  
Target Effects

Increasing natural ingredient awareness and utilization has created an increased demand for sources of natural medicinal ingredients, including sources of compound used to treat malaria. Streptomyces is a genus of prokaryote well recognized for its production of antibiotics and other pharmaceutically useful compound. This study aimed to assess the ability of unpurified fermentation metabolites to inhibit Plasmodium parasites. A strain of bacteria identified as Streptomyces hygroscopicus subsp. hygroscopicus strain i18 were isolated from pineapple fields in Lampung province, and was cultured and fermented on liquid synthetic Gause medium for 10 days. The supernatant was separated from the cells and extracted with ethyl acetate-methanol (1:1). Plasmodium falciparum 3D7 was used for antiplasmodial testing. Metabolites were tested qualitatively using a phytochemical approach. Saponins and triterpenoids were found to be present in the extract. Parasite inhibition as measured using probit analysis and yielded an IC50 value of 11.07 g.m/L. These findings suggest further examinations of this extract (e.g. assessment of off-target effects) are warranted.

scholarly journals CYTOTOXIC EFFECT FROM ETHYL ACETATE-METHANOL SUBFRACTION OF CARRISA CARANDAS L TOWARD HELA CELLS BY IN VITRO TEST

2017 ◽  
Vol 10 (14) ◽  
pp. 21
Author(s):  
Mamik P. Rahayu ◽  
Reslely Harjanti ◽  
Mae S. H. Wahyuningsih ◽  
Supargiyono .
Keyword(s):  
Ethyl Acetate ◽  
Colorless Crystal ◽  
Cytotoxic Effect ◽  
Chloroform Fraction ◽  
In Vitro Test ◽  
Preventive Effect ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Vitro Test

Objective: Cervical cancer is a malignant type of cancer, often affects women, particularly in developing countries. Carrisa carandas leaves contained many secondary metabolites that had potency as an anticancer. The purpose of this study was to understand the cytotoxic effect of subfraction of Carrisa carandas leaves against HeLa cells.Methods: Chloroform fraction was separated by VLC gradually with n-hexane–chloroform–ethyl acetate and methanol. The same profiles from eluent chloroform–ethyl acetate composed fraction 18-26 were categorized as Fr4 and ethyl acetate-methanol composed fraction 27-30 as Fr5. The cytotoxic effect was evaluated by MTT assay on HeLa cellsResults: The result showed that the cytotoxic effect of subfraction Fr4 and Fr5 had IC50 values of 177 mg/ml and 98 mg/ml, respectively. Colorless crystal of Subfraction Fr 5-3 had IC50 value of 333 mg/ml. Subfraction Fr 5 showed effective cytotoxic activity than the others. Conclusion: It had chemo-preventive effect against cancer cellsConclusion: This study applied MTT (Microculture Tetrazolium) method by in vitro test. The advantages of this method are relatively rapid, sensitive and accurate

scholarly journals A SYBR Green 1-based in vitro test of susceptibility of Ghanaian Plasmodium falciparum clinical isolates to a panel of anti-malarial drugs

2013 ◽  
Vol 12 (1) ◽  
Author(s):  
Neils B Quashie ◽  
Nancy O Duah ◽  
Benjamin Abuaku ◽  
Lydia Quaye ◽  
Ruth Ayanful-Torgby ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Sybr Green ◽  
Clinical Isolates ◽  
In Vitro Test ◽  
Vitro Test

Field Evaluation in Kenya of a 48-Hour in Vitro Test for Plasmodium falciparum Sensitivity to Chloroquine *

1983 ◽  
Vol 32 (5) ◽  
pp. 916-921 ◽  
Author(s):  
Harrison C. Spencer ◽  
Phuc Nguyen-Dinh ◽  
Jeffrey D. Chulay ◽  
S. Chemengay Masaba
Keyword(s):  
Plasmodium Falciparum ◽  
Field Evaluation ◽  
In Vitro Test ◽  
Vitro Test

scholarly journals Skrining Aktivitas Antimalaria Beberapa Tanaman Indonesia Hasil Eksplorasi Dari Hutan Raya Cangar, Batu-Malang, Jawa Timur

2017 ◽  
Vol 3 (1) ◽  
pp. 7
Author(s):  
Achmad Fuad Hafid ◽  
Nike Puliansari ◽  
Nur Suci Lestari ◽  
Lidya Tumewu ◽  
Abdul Rahman ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Eucalyptus Globulus ◽  
Antimalarial Activity ◽  
Probit Analysis ◽  
National Forest ◽  
Parasitic Disease ◽  
Antimalarial Therapy ◽  
Ic50 Value ◽  
Stem Extract

Background: Malaria is the most important parasitic disease. Malaria control which depends on specific chemotherapy now complicated by resistance of Plasmodium falciparum to most commonly available antimalarial drug. Such situation has heralded the need for alternative antimalarial therapy. Objective: This research aim was to find new antimalarial candidates from some Indonesia plants collected from Cangar National Forest, Batu-Malang, East Java. Methods: Eleven samples of leaves and stem extracts were screened against Plasmodium falciparum 3D7 culture which maintained in RPMI-1640 Medium. Samples tested in concentration of 0.01, 0.1, 1, 10 and 100 µg/ml. Probit analysis was used to determine IC50. Results: In vitro antimalarial activity revealed that only three crude extracts samples from Fraxinus griffithi stem extract, Piper sulcatum leaves extract and Eucalyptus globulus stem extract had good antimalarial activity with IC50 value of 0.33, 0.20 and 0.55 µg/ml, respectively. Conclusions: Fraxinus griffithi stem extract, Piper sulcatum leaves extract and Eucalyptus globulus stem extract might be a good candidate for antimalarial natural product resources.

PROLONGED INCUBATION IMPROVES THE MICRO-SCALE IN-VITRO TEST FOR DRUG SENSITIVITY OF PLASMODIUM FALCIPARUM

The Lancet ◽  
1984 ◽  
Vol 323 (8367) ◽  
pp. 7-9 ◽  
Author(s):  
StephenL Hoffman ◽  
JamesR Campbell ◽  
HarijaniA Marwoto ◽  
Diet Rustama ◽  
Nyoman Kumara Rai ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Drug Sensitivity ◽  
In Vitro Test ◽  
Prolonged Incubation ◽  
Micro Scale ◽  
Vitro Test

scholarly journals In Vitro Test of Antibacterial Ethanol Extract, n-Hexane Fraction and Ethyl acetate Fraction of Sungkai Leaf (Peronema cenescens) Against Salmonella typhi

1970 ◽  
Vol 8 (3) ◽  
pp. 59-61
Author(s):  
Sitepu Nadroh Br.
Keyword(s):  
Ethyl Acetate ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Salmonella Typhi ◽  
Hexane Fraction ◽  
In Vitro Test ◽  
Leaf Powder ◽  
Vitro Test

Object: This study aims to look at the class of compounds and the comparison of the antibacterial activity of ethanol extract, n-hexane fraction and ethyl acetate of Sungkai leaves against Salmonella typhi. Methods: Study included phytochemical screening and in vitro antibacterial testing of ethanol extract, n-hexane fraction and ethyl acetate of Sungkai leaves against Salmonella typhi. Results: obtained groups of chemical compounds alkaloids, flavonoids, glycosides, anthraquinones, tannins and triterpenoids/steroids on Sungkai leaf powder. Ethanol extract of Sungkai leaves obtained resistance at a concentration of 20% by 12.7 mm, and inhibition of the ethyl acetate fraction at a concentration of 20% of 14.8 mm. Conclusion: Ethyl acetate fraction of Sungkai leaves have antibacterial properties against S. typhi which is greater than ethanol extract and hexane fraction of leaf heal.  

scholarly journals In Vitro Test of Anticalculi Effect from Ethyl Acetate Fraction of Chives Leaf (Allium schoenoprasum L.)

2019 ◽  
Vol 2 (2) ◽  
pp. 20-23
Author(s):  
Iksen ◽  
Siti Morin Sinaga ◽  
Kevin Kevin ◽  
Marshinta Romarta Uly Hutabalian
Keyword(s):  
Ethyl Acetate ◽  
Kidney Stones ◽  
Human Kidney ◽  
Ethyl Acetate Fraction ◽  
Atomic Absorption Spectrophotometry ◽  
In Vitro Test ◽  
Absorption Spectrophotometry ◽  
Allium Schoenoprasum ◽  
Vitro Test

Abstract. The purpose of this study was to analyze the ability of chives leaves of ethyl acetate fraction to dissolve calcium in human kidney stones. This research starts from making the ethyl acetate fraction, calibration curve for calcium and measuring dissolved calcium levels in kidney stones by using atomic absorption spectrophotometry. The results showed that the ethyl acetate fraction with a concentration of 2.5% had the greatest anti-calculi capability of 92.02%. The conclusion of this study is that the ethyl acetate fraction of chives leaf has the potential to be anticalculi in the treatment of kidney stones.   Abstrak. Tujuan dari penelitian ini adalah untuk menganalisis kemampuan fraksi etil asetat daun kucai  untuk melarutkan kalsium pada batu ginjal manusia. Penelitian ini dimulai dari pembuatan fraksi etil asetat, pembuatan kurva kalibrasi kalsium serta pengukuran kadar kalsium terlarut pada batu ginjal dengan menggunakan spektrofotometri serapan atom. Hasil penelitian menunjukkan bahwa fraksi etil asetat dengan konsentrasi 2,5 % memiliki kemampuan antikalkuli yang terbesar yakni 92,02%. Kesimpulan penelitian ini adalah bahwa fraksi etil asetat daun kucai berpotensi sebagai antikalkuli pada pengobatan batu ginjal.  

A Rapid in Vitro Test for Chloroquine-Resistant Plasmodium falciparum

1990 ◽  
Vol 42 (6) ◽  
pp. 521-526 ◽  
Author(s):  
Ilya Y. Gluzman ◽  
Kano Nkangineme ◽  
Augustine U. Orjih ◽  
J. Tyler Martin ◽  
Thomas E. Wellems ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Test ◽  
Vitro Test

scholarly journals THE EFFECT OF MALACCA LEAVES (Phyllantus emblica) ETHANOLIC EXTRACT ON Plasmodium falciparum GROWTH IN VITRO

2018 ◽  
Vol 12 (4) ◽  
Author(s):  
Nuzul Asmilia ◽  
T Armansyah TR ◽  
Dwinna Aliza ◽  
Juli Melia ◽  
Erdiansyah Rahmi ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Antiplasmodial Activity ◽  
Control Group ◽  
Ic50 Value

The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.

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