scholarly journals Optimization and Molecular Characterization of Lipase Producing Bacillus subtilis Strain Rcps3 and Bacillus fumarioli Strain Rcps4 from Oil-contaminated Soils of Warangal

2020 ◽  
Vol 14 (3) ◽  
pp. 2063-2074
Author(s):  
Ramya Chouhan ◽  
Suresh Lapaka ◽  
Nagaraju Alpula ◽  
Srinivas Podeti
Keyword(s):  
Bacillus Subtilis ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Lipase Production ◽  
Subtilis Strain ◽  
Bacterial Strains ◽  
Almost All ◽  
Bacterial Sources ◽  
Hydrolysis Of

Microbial sources are regularly used as reliable biocatalysts sources which are often used in the process and production industry. Demands for such organisms with greater capacity of intended enzyme production are on the rise. Lipase is important enzyme used in the biotechnological process of hydrolysis of fats in almost all the relevant industries We have utilized the local oil-contaminated soil resources to search for efficacious bacterial strains that have excellent lipase activity. We were successful in identifying two such bacterial sources, namely, Bacillus subtilis strain RCPS3 and Bacillus fumarioli strain RCPS4, responsible for lipase production from oil effluent contaminated soil of Telangana. This is the first report of these two strains from this part of India that are involved in lipase production. The strains were isolated, optimized, and purified using standard microbiology protocols and were characterized at the molecular level using the biomarker 16s ribosomal RNA genes of the strains. The identified and isolated bacterial strains were confirmed as Bacillus subtilis strain RCPS3, and Bacillus fumarioli strain RCPS4 through molecular and computational characterization.

scholarly journals Enhanced Production of Biosurfactant from Bacillus subtilis Strain Al-Dhabi-130 under Solid-State Fermentation Using Date Molasses from Saudi Arabia for Bioremediation of Crude-Oil-Contaminated Soils

2020 ◽  
Vol 17 (22) ◽  
pp. 8446
Author(s):  
Naif Abdullah Al-Dhabi ◽  
Galal Ali Esmail ◽  
Mariadhas Valan Arasu
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Crude Oil ◽  
Solid State Fermentation ◽  
Contaminated Soils ◽  
Quadratic Model ◽  
Subtilis Strain ◽  
Natural Environments ◽  
Bacterial Strains ◽  
Enhanced Production

Crude oil and its derivatives are the most important pollutants in natural environments. Bioremediation of crude oil using bacteria has emerged as a green cleanup approach in recent years. In this study, biosurfactant-producing Bacillus subtilis strain Al-Dhabi-130 was isolated from the marine soil sediment. This organism was cultured in solid-state fermentation using agro-residues to produce cost-effective biosurfactants for the bioremediation of crude-oil contaminated environments. Date molasses improved biosurfactant production and were used for further optimization studies. The traditional “one-variable-at-a-time approach”, “two-level full factorial designs”, and a response surface methodology were used to optimize the concentrations of date molasses and nutrient supplements for surfactant production. The optimum bioprocess conditions were 79.3% (v/w) moisture, 34 h incubation period, and 8.3% (v/v) glucose in date molasses. To validate the quadratic model, the production of biosurfactant was performed in triplicate experiments, with yields of 74 mg/g substrate. These findings support the applications of date molasses for the production of biosurfactants by B. subtilis strain Al-Dhabi-130. Analytical experiments revealed that the bacterial strain degraded various aromatic hydrocarbons and n-alkanes within two weeks of culture with 1% crude oil. The crude biosurfactant produced by the B. subtilis strain Al-Dhabi-130 desorbed 89% of applied crude oil from the soil sample. To conclude, biosurfactant-producing bacterial strains can increase emulsification of crude oil and support the degradation of crude oil.

scholarly journals Intracellular serine proteinase of Bacillus subtilis strain Marburg 168. Comparison with the homologous enzyme from Bacillus subtilis strain A-50

1979 ◽  
Vol 179 (2) ◽  
pp. 333-339 ◽  
Author(s):  
A Y Strongin ◽  
D I Gorodetsky ◽  
I A Kuznetsova ◽  
V V Yanonis ◽  
Z T Abramov ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Serine Proteinase ◽  
Subtilis Strain ◽  
Bacterial Strains ◽  
Terminal Sequence ◽  
Gramicidin S ◽  
Intracellular Enzymes ◽  
Sepharose 4B ◽  
Homologous Enzyme ◽  
Strict Selection

Intracellular serine proteinase was isolated from sporulating cells of Bacillus subtilis Marburg 168 by gramicidin S-Sepharose 4B affinity chromatography. The enzymological characteristics, the amino acid composition and the 19 residues of the N-terminal sequence of the enzyme are reported. The isolated proteinase was closely related to, but not completely identical with, the intracellular serine proteinase of B. subtilis A-50. The divergence between these two intracellular enzymes was less than that between the corresponding extracellular serine proteinases (subtilisins) of types Carlsberg and BPN′!, produced by these bacterial strains. This may be connected with the more strict selection constraints imposed in intracellular enzymes during evolution.

scholarly journals Potential risks of antibiotic resistant bacteria and genes in bioremediation of petroleum hydrocarbon contaminated soils

2020 ◽  
Vol 22 (5) ◽  
pp. 1110-1124 ◽  
Author(s):  
Colin J. Cunningham ◽  
Maria S. Kuyukina ◽  
Irena B. Ivshina ◽  
Alexandr I. Konev ◽  
Tatyana A. Peshkur ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbon ◽  
Resistant Bacteria ◽  
Careful Selection ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
Potential Risks ◽  
Selection Of

The problems associated with potential risks of antibiotic resistance spreading during bioremediation of oil-contaminated soil are discussed. Careful selection of bacterial strains and pretreatment of organic wastes used as fertilizers are suggested.

scholarly journals Bacillus subtilis Strain Engineered for Treatment of Soil-Transmitted Helminth Diseases

2013 ◽  
Vol 79 (18) ◽  
pp. 5527-5532 ◽  
Author(s):  
Yan Hu ◽  
Melanie M. Miller ◽  
Alan I. Derman ◽  
Brian L. Ellis ◽  
Rose Gomes Monnerat ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Strain ◽  
Subtilis Strain ◽  
Human Consumption ◽  
Bacterial Strains ◽  
Content Type ◽  
Live Bacteria ◽  
Therapeutic Properties ◽  
Adverse Environmental Conditions ◽  
Hookworm Infections

ABSTRACTSoil-transmitted helminths (hookworms, whipworms, and large roundworms) are agents of intestinal roundworm diseases of poverty that infect upwards of 2 billion people worldwide. A great challenge in treating these diseases is the development of anthelmintic therapeutics that are inexpensive, can be produced in great quantity, and are capable of delivery under varied and adverse environmental conditions. A potential solution to this challenge is the use of live bacteria that are acceptable for human consumption, e.g.,Bacillus subtilis, and that can be engineered with therapeutic properties. In this study, we expressed theBacillus thuringiensisanthelmintic protein Cry5B in a bacterial strain that has been used as a model for live bacterial therapy,Bacillus subtilisPY79. PY79 transformed with a Cry5B expression plasmid (PY79-Cry5B) is able to express Cry5B from the endogenousB. thuringiensis cry5Bpromoter. During sporulation of PY79-Cry5B, Cry5B is packaged as a crystal. Furthermore, Cry5B produced in PY79 is bioactive, with a 50% lethal concentration (LC50) of 4.3 μg/ml against the roundwormCaenorhabditis elegans. PY79-Cry5B was a significantly effective therapeutic in experimentalAncylostoma ceylanicumhookworm infections of hamsters. A single 10-mg/kg (0.071 μmol/kg of body weight) dose of Cry5B administered as a Cry5B-PY79 spore crystal lysate achieved a 93% reduction in hookworm burdens, which is superior on a molar level to reductions seen with clinically used anthelmintics. Given that a bacterial strain such as this one can be produced cheaply in massive quantities, our results demonstrate that the engineering and delivery of live bacterial strains have great potential to treat a significant contributor to poverty worldwide, namely, hookworm disease and other soil-transmitted helminthiasis.

scholarly journals Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29

2009 ◽  
Vol 10 (4) ◽  
pp. 264-272 ◽  
Author(s):  
Jing Li ◽  
Qian Yang ◽  
Li-hua Zhao ◽  
Shu-mei Zhang ◽  
Yu-xia Wang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Antifungal Protein ◽  
Subtilis Strain ◽  
Purification And Characterization

scholarly journals RHIZODEGRADATION OF PHENANTHRENE, ANTHRACENE AND PYRENE BY AUGMENTING BACILLUS CEREUS AND BACILLUS SUBTILIS STRAINS

2021 ◽  
Vol 9 (02) ◽  
pp. 89-96
Author(s):  
M. Poornachander Rao ◽  
◽  
Anitha Yerra ◽  
K. Satyaprasad ◽  
◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Contaminated Soils ◽  
Rhizosphere Soil ◽  
Culture Method ◽  
Experimental Period ◽  
Bacterial Strains ◽  
Bacterial Populations ◽  
Metabolic Activities ◽  
Pot Culture

Rhizodegradation is one of the best methods for the effective removal of dangerous polycyclic aromatic hydrocarbons pollutants from soil. This is operative due to the high persistent, non-bioavailability nature of PAHs and combined, sequential reactions of bacteria present in rhizosphere of plants. We have conducted pot-culture method to study the degradation of three PAHs compounds namely phenanthrene, anthracene and pyrene in artificially contaminated soils of rhizosphere and non-rhizosphere soil treatments of blackgram(Vignamungo L.) that augmented by two potential PAHs degraders namely Bacillus cereus CPOU13 and Bacillus subtilis SPC14 isolated from naturally contaminated soils for 90days. HPLC studies revealed that degradation percentages of the three PAHs in treatments were more where selected strains augmented to the soil treatments over the non-augmented soils. The rhizosphere treatments that have augmented strains recorded more degradation percentages of phenanthrene, anthracene and pyrene over the rhizosphere treatments that were non-augmented. Pyrene, a high molecular weight PAHs degraded maximum to 96.24% in rhizosphere soil treatment that is augmented with the strains while moderate degradation of pyrene recorded in non-autoclaved soil treatments that contain natural microbial communities. The study of counting of bacterial populations during the experimental period revealed that the populations of the selected and other natural bacteria were gradually increased from the first day, reached maximum by 60days and became almost consistent in 90days in all the treatments. It was also observed that the populations of bacteria were high in rhizosphere treatments compared to the non-rhizosphere soil treatments. With these results it has been predicted that degradation of PAHs in rhizosphere soil treatments is closely associated with the increasing PAHs degrading bacterial populations of selected bacterial strains that may consume more quantity of PAHs for their metabolic activities in rhizosphere soils. Key words: Rhizodegradation, PAHs, HPLC, pot culture.

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