scholarly journals Screening, Isolation and Identification of Thermophilic Esterase Enzyme Isolated from Rhodococcus SP: LKE-021

2019 ◽  
Vol 13 (3) ◽  
pp. 1855-1861 ◽  
Author(s):  
Lekha Singh ◽  
Gaurav Sharma ◽  
Gyanendra Awasthi ◽  
Lokendra Kumar ◽  
Mohammad Irfan Ali ◽  
...  
Keyword(s):  
Isolation And Identification ◽  
Thermophilic Esterase ◽  
Rhodococcus Sp

scholarly journals POTENSI PIGMEN ALAMI DARI BAKTERI SIMBION KARANG Mantipora sp SEBAGAI PEWARNA MAKANAN

Pro Food ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 643
Author(s):  
Dhanang Puspita ◽  
Jacob Lukas Alexander Uktolseja
Keyword(s):  
Coral Reefs ◽  
Research Method ◽  
Food Industry ◽  
Synthetic Dyes ◽  
Bacterial Isolation ◽  
Isolation And Identification ◽  
Natural Pigment ◽  
Self Defense ◽  
Bacterial Pigment ◽  
Rhodococcus Sp

ABSTRACT Color is very important in the food industry. The need for food coloring requires manufacturers to use synthetic dyes that have the potential to cause poisoning and cancer. One source of natural dyes comes from bacteria that are symbiotic with coral reefs. The purpose of this study is to isolate and characterize the bacterial pigment that has symbiosis with Montipora sp. The research method consisted of bacterial isolation and identification, pigment identification with UV-Vis spectrofotometer (200 – 800 nm) and TLC. The results of isilation and identification showed that Rhodococcus sp is dominant bacterial which is produces of carotenoiids for self defense from UV rays. The pigment found in Rhodococcus sp has the potential as a natural pigment for food coloring. Keywords: carotenoids, Montipora, pigment, Rhodococcus sp. ABSTRAK Warna sangat penting dalam industri pangan. Kebutuhan pewarna makanan menuntut produsen memakai bahan pewarna sintetik yang berpotensi menyebabkan keracunan dan kanker. Salah satu sumber pewarna alami berasal dari bakteri yang bersimbiosis dengan terumbu karang. Tujuan dari penelitian ini adalah mengisolasi dan mengkarakterisasi pigmen bakteri yang bersimbion Montipora sp. Metode penelitian terdiri dari isolasi dan identifkasi bakteri, identifikasi pigmen dengan spektrofotometer UV-Vis (200 – 800 nm) dan KLT. Hasil isolasi dan identifikasi bakteri berjenis Rhodococcus sp dan piigmen yang dihasilkan adalah karotenoid yang digunakan sebagai pertahanan diri dari sinar UV. Pigmen yang terdapat pada Rhodococcus sp berpotensi sebagai pigmen alami untuk pewarna pangan. Kata kunci: karotenoid, Montipora, pigmen, Rhodococcus sp.

scholarly journals Isolation and identification of berberine and berberrubine metabolites by berberine-utilizing bacterium Rhodococcus sp. strain BD7100

2016 ◽  
Vol 80 (5) ◽  
pp. 856-862 ◽  
Author(s):  
Kazuki Ishikawa ◽  
Hisashi Takeda ◽  
Daigo Wakana ◽  
Fumihiko Sato ◽  
Tomoo Hosoe
Keyword(s):  
Isolation And Identification ◽  
Rhodococcus Sp

Isolation and identification of a novel Rhodococcus sp. ML-0004 producing epoxide hydrolase and optimization of enzyme production

2007 ◽  
Vol 42 (5) ◽  
pp. 889-894 ◽  
Author(s):  
Zhiqiang Liu ◽  
Yin Li ◽  
Lifeng Ping ◽  
Yingying Xu ◽  
Fengjie Cui ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Epoxide Hydrolase ◽  
Isolation And Identification ◽  
Rhodococcus Sp

Isolation and identification of chemical constituents from the roots of Peucedanum praeruptorum

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
YJ Lee ◽  
J Kim ◽  
J Lee ◽  
ES Cho ◽  
OS Bang
Keyword(s):  
Chemical Constituents ◽  
Isolation And Identification

Isolation and identification of neuroprotective compounds from Machilus thunbergii

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
CJ Ma ◽  
KY Lee ◽  
HK Yang ◽  
JS Yoon ◽  
YC Kim ◽  
...  
Keyword(s):  
Isolation And Identification ◽  
Machilus Thunbergii

Isolation of post operative hospital acquired infections in a private hospital of Bangladesh and identification of clonal relatedness of E. coli by using Pulse filed gel electrophoresis

2012 ◽  
Vol 6 (2) ◽  
pp. 7-10
Author(s):  
Mohammad Murshed ◽  
Sabeena Shahnaz ◽  
Md. Abdul Malek
Keyword(s):  
Private Hospital ◽  
Isolation And Identification ◽  
College Hospital ◽  
Hospital Acquired Infection ◽  
E Coli ◽  
Hospital Acquired Infections ◽  
Medical College ◽  
Clonal Relatedness ◽  
Hospital Acquired ◽  
Dressing Materials

Isolation and identification of post operative hospital acquired infection was carried out from July 2008 to December 2008 in Holy Family Red Crescent Medical College Hospital (private hospital). The major pathogen of wound infection was E. coli. A total; of 120 samples were collected from the surrounding environment of post operative room like floor, bed sheets, instruments, dressing materials, catheter, nasogastric and endotracheal tube. E. coli (40%) was the predominant organism followed by S. aureus (24%). DNA fingerprinting analysis using pulsed field gel electreopheresis of XbaI restriction digested genomic DNA showed that clonal relatedness between the two clinical nd environmental isolates were 100%.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19369 Bangladesh J Med Microbiol 2012; 06(02): 7-10

Investigation of duck plague virus in hoar areas of Bangladesh

2020 ◽  
Vol 26 (1-2) ◽  
pp. 73-78
Author(s):  
A Hossen ◽  
MH Rahman ◽  
MZ Ali ◽  
MA Yousuf ◽  
MZ Hassan ◽  
...  
Keyword(s):  
Cytopathic Effect ◽  
Clinical Sample ◽  
Chorioallantoic Membrane ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Duck Plague Virus ◽  
Pcr Method ◽  
Polymerase Chain ◽  
The Individual ◽  
Free Ranging

Duck plague (DP) is the most important infectious disease of geese, ducks and free-ranging water birds. The present study was conducted to determine the prevalence of duck plague virus followed by isolation and identification. For these purposes, a total of 155 cloacal swabs samples were collected randomly from duck of different haor areas of Bangladesh including 45 (41 surveillance and 4 clinical) samples from Netrokona; 42 (40 surveillance and 2 clinical) samples from Kishoregonj; 30 samples from Brahmanbaria and 38 samples from Sunamganj. The samples were processed and pooled (1:5 ratio) for initial screening of target polymerase gene of duck plague virus by polymerase chain reaction (PCR) method. All the samples of a positive pool were then tested individually for identifying the individual positive samples. The result showed that out of 155 samples, 41 (26.45%) were found positive in which 17 were from Netrokona, where 15 (36.58%) were from surveillance samples and 2 (50%) were from clinical sample; 16 were from Kishoregonj, where 14 (35%) were from surveillance samples and 2 (100%) were from clinical sample; 2 (6.6%) were from Brahmanbaria and 5 (13.15%) were from Sunamganj. These positive samples were inoculated into 9-10 days embryonated duck eggs (EDE) through chorioallantoic membrane (CAM) route for the isolation of virus. The EDE died earlier was also chilled, and in a similar way, the CAMs were collected and again performed PCR for id entification of virus. Out of 41 PCR positive samples, 26 samples were isolated and reconfirmed by PCR. Subsequently, DPV was isolated in primary duck embryo fibroblasts cell culture and confirmed by observing cytopathic effect (CPE). Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 73-78

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