scholarly journals Isolation and Characterization of Marine Endophytic Fungi from Seaweeds, and Bioactivity of their Crude Extracts

2019 ◽  
Vol 13 (3) ◽  
pp. 1451-1460 ◽  
Author(s):  
Feroze Ahamed ◽  
Marudhamuthu Murugan
Keyword(s):  
Endophytic Fungi ◽  
Isolation And Characterization ◽  
Crude Extracts

scholarly journals Isolation and characterization of glyoxylate dehydrogenase from the fungus Sclerotium rolfsii

1984 ◽  
Vol 218 (1) ◽  
pp. 113-118 ◽  
Author(s):  
A J Balmforth ◽  
A Thomson
Keyword(s):  
Affinity Chromatography ◽  
Active Site ◽  
Sclerotium Rolfsii ◽  
Thiol Group ◽  
Isolation And Characterization ◽  
Crude Extracts ◽  
Nad Oxidoreductase ◽  
Inhibition Studies ◽  
Dye Affinity Chromatography

Glyoxylate dehydrogenase (glyoxylate: NAD+ oxidoreductase) was purified 600-fold in three steps from crude extracts of the fungus Sclerotium rolfsii (Corticium rolfsii Curzi). Two of the purification steps involved dye-affinity chromatography. The enzyme is a tetramer of Mr 250 000, with identical subunits of Mr 57 000. Inhibition studies suggest that there is one essential thiol group per active site.

scholarly journals Isolation and characterization of a low-molecular-weight substance activating head and bud formation in hydra

Development ◽  
1973 ◽  
Vol 29 (1) ◽  
pp. 27-38
Author(s):  
H. Chica Schaller
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Bud Formation ◽  
Isolation And Characterization ◽  
Crude Extracts ◽  
Head Formation ◽  
Weight Substance

From crude extracts of hydra, a substance activating head formation was isolated and enriched at least 100000-fold. The molecular weight was determined to be approximately 900. Sensitivity against proteases suggests that it is a peptide. The substance acts at a concentration equivalent to the extract of 1 hydra per ml or at a concentration of less than 10 10M. In its highly purified form the substance activates head and bud formation.

scholarly journals Isolation and characterization of plumbagin (5- hydroxyl- 2- methylnaptalene-1,4-dione) producing endophytic fungi Cladosporium delicatulum from endemic medicinal plants

2018 ◽  
Vol 20 ◽  
pp. e00282 ◽  
Author(s):  
N. Venkateswarulu ◽  
S. Shameer ◽  
P.V. Bramhachari ◽  
S.K. Thaslim Basha ◽  
C. Nagaraju ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Endophytic Fungi ◽  
Isolation And Characterization

Isolation and characterization of endophytic fungi from Camptotheca acuminata

2013 ◽  
Vol 29 (10) ◽  
pp. 1831-1838 ◽  
Author(s):  
Xiaowei Ding ◽  
Kaihui Liu ◽  
Baiwan Deng ◽  
Wenqiang Chen ◽  
Wenjun Li ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Camptotheca Acuminata ◽  
Isolation And Characterization

scholarly journals The isolation and characterization of 3-phosphoglycerate dehydrogenase from peas

1968 ◽  
Vol 109 (5) ◽  
pp. 743-748 ◽  
Author(s):  
J. C. Slaughter ◽  
D. D. Davies
Keyword(s):  
Thiol Group ◽  
Isolation And Characterization ◽  
Crude Extracts ◽  
Michaelis Constants ◽  
Phosphoglycerate Dehydrogenase

1. 3-Phosphoglycerate dehydrogenase was purified 400-fold from crude extracts of etiolated pea epicotyls. 2. Michaelis constants were determined for all four substrates. 3. Loss of sensitivity to inhibition by l-serine occurs on purification. 4. The purified enzyme is inhibited by thiol-group reagents and, with N-ethyl-maleimide, protection is afforded by 3-phosphoglycerate though not by NAD+.

scholarly journals Isolasi dan Karakterisasi Fungi Endofit Umbi Porang (Amorphophallus muelleri Blume) sebagai Antibakteri

BIOSCIENTIAE ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 55
Author(s):  
Muhammad Fazrul Rahman ◽  
Witiyasti Imaningsih ◽  
Sasi Gendro Sari
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Endophytic Fungi ◽  
E Coli ◽  
Isolation And Characterization ◽  
Venomous Animal ◽  
Animal Bites ◽  
Aspergillus Sp ◽  
Escherichia Coli Bacteria

Medicinal plants such as porang (Amorphophallus muelleri Blume) can produce bioactive compounds from plants-associated endophytes. Therefore, medicinal plants were a sources of isolation of endophytic fungi and endophytic fungi were a sources of secondary metabolites that have anticancer, antimalarial, antimicrobial, and so on. A Porang tuber has been used as a medicine for boils, medicine for sliced wounds and medicine for wounds due to venomous animal bites. The research was aimed to carry out isolation and characterization of endophytic fungi from porang tuber, and to test the ability of endophytic fungi from porang tuber as antibacterial against gram positive (Staphylococcus aureus) and gram negative (Escherichia coli) bacteria. Isolation of endophytic fungi from porang tubers succeeded in getting five different fungi isolates. Based on macroscopic and microscopic characteristics, endophytic fungi isolated from porang tuber were members of the genus Curvularia, Penicillium, 2 isolates of Aspergillus, and 1 isolate that had not been identified. Porang endophytic fungi had antibacterial activity against S. aureus, but did not have antibacterial activity against E. coli bacteria. Endophytic fungi that had antibacterial activity were Curvularia sp., Penicillium sp., Aspergillus sp.

scholarly journals Isolation and Characterization of Endophytic Fungi from Purslane and the Effects of Isolates on the Growth of the Host

2019 ◽  
Vol 09 (05) ◽  
pp. 438-453
Author(s):  
Mandlaa   ◽  
Yu Zhang ◽  
Yongqing Wan ◽  
Ying Tie ◽  
Bo Zhang ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Isolation And Characterization
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