scholarly journals New quantitative automated model to simulate bacterial dissemination in human tissue during irrigation of contaminated wounds

2021 ◽  
Vol 42 ◽  
pp. 63-71
Author(s):  
C Brochhausen ◽  
◽  
F Froschermeier ◽  
V Alt ◽  
C Pfeifer ◽  
...  
Keyword(s):  
High Pressure ◽  
Cost Effective ◽  
Detection Algorithm ◽  
Negative Influence ◽  
Positive Control ◽  
Deep Tissue ◽  
Tissue Samples ◽  
Tissue Healing ◽  
Bioimage Analysis ◽  
Measuring Field

This study presents a simple and cost-effective model using microparticles to simulate the bacterial distribution pattern in soft tissue after low- and high-pressure irrigation. Silica coated iron microparticles [comparable diameter (1 µm) and weight (0.8333 pg) to Staphylococcus aureus] were applied to the surface of twenty fresh human muscle tissue samples in two amputated lower legs. Particle dissemination into deep tissue layers as an undesired side effect was investigated in four measuring fields as positive control (PC) as well as after performing pulsatile high-pressure (HP, 8 measuring fields) and low-pressure flushing (LP, 8 measuring fields). Five biopsies were taken out of each measuring field to get a total number of 100 biopsies. After histological and digital image processing, the specimens were analysed, and all incomplete sections were excluded. A special detection algorithm was parameterised using the open source bioimage analysis software QuPath. The application of this detection algorithm enabled automated counting and detection of the particles with a sensitivity of 95 % compared to manual counts. Statistical analysis revealed significant differences (p < 0.05) in our three different sample groups: HP (M = 1608, S = 302), LP (M = 2176, SD = 609) and PC (M = 4011, SD = 686). While both HP and LP flushing techniques are able to reduce the number of bacteria, a higher effectiveness is shown for HP irrigation. Nevertheless, a challenge for the validity of the study is the use of dead tissue and therefore a possible negative influence of high-pressure irrigation on tissue healing and further dispersion of particles cannot be evaluated.

scholarly journals Development of cost-effective IoT module-based pipe classification system for flexible manufacturing system of painting process of high-pressure pipe

2021 ◽  
Author(s):  
Young Jun Yoo
Keyword(s):  
High Pressure ◽  
Classification System ◽  
Flexible Manufacturing ◽  
Manufacturing System ◽  
Flexible Manufacturing System ◽  
Cost Effective ◽  
Detection Algorithm ◽  
Line Detection ◽  
Painting Robot ◽  
Painting Process

Abstract This paper researches an IoT module-based pipe classification system for a flexible manufacturing system that recognizes the size and length of pipes used in the painting process of high-pressure pipes. The proposed system is composed of an IoT module, USB camera, and edge TPU for pipe classification. The proposed system recognizes the type of pipe by three processes; object detection of the pipe, line detection algorithm of the three regions of interest, and pipe classification algorithm based on the line detection algorithm. Furthermore, the proposed system enables web-based real-time monitoring, providing convenience to workers and helping them make quick decisions. The IoT module interfaces with the painting robot and the sequence control that paints for each type of pipe is executed in the painting robot, allowing flexible manufacturing of the painting process.

Insight on Ameliorative Role of Selenium Nanoparticles and Niacin in Wound Healing on Adult Female Albino Mice

2020 ◽  
Vol 14 (3) ◽  
pp. 169-186
Author(s):  
Marwa Emam ◽  
Akaber T. Keshta ◽  
Yasser M.A. Mohamed ◽  
Yasser A. Attia
Keyword(s):  
Wound Healing ◽  
Adult Female ◽  
Vascular Endothelial Cell ◽  
Skin Layer ◽  
Positive Control ◽  
Healing Time ◽  
Selenium Nanoparticles ◽  
Tissue Samples ◽  
Liver And Kidney ◽  
Albino Mice

Background: Wound healing is a complex process necessary for repairing damaged tissues and preventing infection. Selenium nanoparticles (Se NPs) were known due to their antioxidant and antimicrobial effects, also niacin has angiogenesis and antioxidant effects that are important in wound healing. Objective: The present study was conducted to investigate the effect of Se NPs and niacin in reducing and accelerating the wound healing time in mice. Methods: A simple wet chemical method has been modified to synthesize Se NPs in order to investigate their effect and niacin on reducing the wound healing in 80 adult female albino mice (250 mm2 full thickness open excision wound) that were divided into eight groups (10 mice/each). After 30-days, the mice were sacrificed, blood and tissue samples were taken for analysis. Results: The results showed that the percentage of wound area had been significantly reduced in Se NPs and niacin treated groups compared to the positive control. The level of Vascular Endothelial cell Growth Factor and Collagenase I in Se NPs and niacin groups significantly exceed those of other groups while Nitric Oxide (NO) was significantly decreased in treated groups. Liver and kidney functions showed the lower toxicity effect of Se NPs and niacin. Skin tissue showed the wound healing effect of Se NPs and niacin by regenerating skin layer compared to the positive group. Conclusion: Se NPs and niacin play an important role in accelerating and reducing the time of wound healing while they were antagonistic to each other.

scholarly journals Autologous Platelet-Rich Fibrin (PRF) as an Adjunct in the Management of Osteoradionecrosis and Medication-Related Osteonecrosis of Jaws. Case Series in A Single Centre

2021 ◽  
Vol 11 (8) ◽  
pp. 3365
Author(s):  
Benjie Law ◽  
Hui Yuh Soh ◽  
Syed Nabil ◽  
Rama Krsna Rajandram ◽  
Abd Jabar Nazimi ◽  
...  
Keyword(s):  
Case Series ◽  
Cost Effective ◽  
Control Group ◽  
Platelet Rich Fibrin ◽  
Tissue Healing ◽  
Number Of Patients ◽  
Short Period ◽  
Oral And Maxillofacial Region ◽  
Surgical Adjunct ◽  
Autologous Platelet

Osteoradionecrosis (ORN) of the jaws and medication-related osteonecrosis of the jaws (MRONJ) are uncommon but serious diseases affecting the oral and maxillofacial region with clinically similar appearance but distinct pathophysiology. Management of ORN and MRONJ is inherently challenging and the treatment outcomes are unpredictable. The use of autologous platelet concentrates (APCs) to promote hard and soft tissue healing is well described in the literature, and the efficacy of leucocyte and platelet-rich fibrin (L-PRF) has been well documented in a number of clinical studies. The aim of this study was to present our treatment strategy and the outcomes of incorporating L-PRF as a surgical adjunct in management of ORN and MRONJ in our centre. Methods: eight cases of ORN and MRONJ were treated with a combination of sequestrectomy and L-PRF as a surgical adjunct. Results: the overall success was 87.5%. Using L-PRF as an adjunct, we were able to predictably manage ORN and MRONJ without causing significant morbidity. Conclusion: our experience shows that L-PRF may be used as a valuable and cost-effective adjunct to surgical management of ORN and MRONJ. However, due to a limited number of patients, and a short period of review, the true effectiveness of the method is yet to be demonstrated in a longer follow-up study including a greater number of patients, besides the inclusion of a control group.

scholarly journals Single-Run Separation and Quantification of 14 Cannabinoids Using Capillary Electrophoresis

Separations ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 30
Author(s):  
Emil A. Zaripov ◽  
Tiah Lee ◽  
Yuchu Dou ◽  
Cory S. Harris ◽  
Artem Egorov ◽  
...  
Keyword(s):  
High Pressure ◽  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
Sodium Hydroxide ◽  
Limit Of Detection ◽  
Cost Effective ◽  
Uv Detector ◽  
Good Separation ◽  
Method Performance ◽  
Cost Effective Alternative

Quantification of major cannabinoids in cannabis products is normally performed using high-pressure liquid chromatography (HPLC)-based methods. We propose a cost-effective alternative method that successfully separates and quantifies 14 cannabinoids in a single run using capillary electrophoresis (CE) coupled with a UV detector in 18 min. The separation is carried out in 60% acetonitrile in the presence of 6.5 mM sodium hydroxide and 25 µM β-cyclodextrin, resulting in good separation of cannabinoids. Our CE method demonstrated the limit of detection between 1.2–1.8 µg/mL, with the linear range reaching up to 50 µg/mL. We validated the method performance by testing a plant extract and quantifying cannabinoid content. This method is the first to separate 14 cannabinoids in one run using a CE system with UV detection.

Utilizing Novel Expandable Steel Packers to Overcome Multi-Stage Fracturing Completion Deployment Challenges in Horizontal Gas Wells

2021 ◽  
Author(s):  
Ebikebena M. Ombe ◽  
Ernesto G. Gomez ◽  
Aldia Syamsudhuha ◽  
Abdullah M. AlKwiter
Keyword(s):  
High Pressure ◽  
High Temperature ◽  
Cost Effective ◽  
Outer Diameter ◽  
Gas Wells ◽  
Multi Stage ◽  
High Pressure High Temperature ◽  
Zonal Isolation ◽  
Gas Bearing ◽  
Productive Time

Abstract This paper discusses the successful deployment of Multi-stage Fracturing (MSF) completions, composed of novel expandable steel packers, in high pressure, high temperature (HP/HT) horizontal gas wells. The 5-7/8" horizontal sections of these wells were drilled in high pressure, high temperature gas bearing formations. There were also washed-outs & high "dog-legs" along their wellbores, due to constant geo-steering required to keep the laterals within the hydrocarbon bearing zones. These factors introduced challenges to deploying the conventional MSF completion in these laterals. Due to the delicate nature of their packer elastomers and their susceptibility to degradation at high temperature, these conventional MSF completions could not be run in such hostile down-hole conditions without the risk of damage or getting stuck off-bottom. This paper describes the deployment of a novel expandable steel packer MSF completion in these tough down-hole conditions. These expandable steel packers could overcome the challenges mentioned above due to the following unique features: High temperature durability. Enhanced ruggedness which gave them the ability to be rotated & reciprocated during without risk of damage. Reduced packer outer diameter (OD) of 5.500" as compared to the 5.625" OD of conventional elastomer MSF packers. Enhanced flexibility which enabled them to be deployed in wellbores with high dog-leg severity (DLS). With the ability to rotate & reciprocate them while running-in-hole (RIH), coupled with their higher annular clearance & tolerance of high temperature, the expandable steel packers were key to overcoming the risk of damaging or getting stuck with the MSF completion while RIH. Also, due to the higher setting pressure of the expandable steel packers when compared to conventional elastomer packers, there was a reduced risk of prematurely setting the packers if high circulating pressure were encountered during deployment. Another notable advantage of these expandable packers is that they provided an optimization opportunity to reduce the number of packers required in the MSF completion. In a conventional MSF completion, two elastomer packers are usually required to ensure optimum zonal isolation between each MSF stage. However, due to their superior sealing capability, only one expandable steel packer is required to ensure good inter-stage isolation. This greatly reduces the number of packers required in the MSF completion, thereby reducing its stiffness & ultimately reducing the probability of getting stuck while RIH. The results of using these expandable steel packers is the successful deployment of the MSF completions in these harsh down-hole conditions, elimination of non-productive time associated with stuck or damaged MSF completion as well as the safe & cost-effective completion in these critical horizontal gas wells.

scholarly journals Tensile Performance of a Novel Glue-Laminated Cornstalk Scrimber

2020 ◽  
Vol 23 (3) ◽  
pp. 198-203
Author(s):  
Wei Tian ◽  
Yongmei Qian ◽  
Ruozhu Wang ◽  
Yiming Wang
Keyword(s):  
High Pressure ◽  
Tensile Strength ◽  
Experimental Analysis ◽  
Building Material ◽  
Mechanical Performance ◽  
Strain Curve ◽  
Cost Effective ◽  
Building Industry ◽  
Young’S Moduli ◽  
Tensile Performance

Glue-laminated cornstalk scrimber is a novel composite to substitute timber. This composite can be prepared in three steps: selecting flawless cornstalks, laying them parallel to grain, and gluing the scrimbers under high pressure. Compared with ordinary timber, glue-laminated cornstalk scrimber excels in the resistance to water, damping, insect, and fire. It is therefore widely recognized as novel eco-friendly and cost- effective composite with great potential in the building industry. The tensile strength of glue-laminated cornstalk scrimber mainly depends on the parallel-to-grain strength of its fibers. The mechanical performance parallel to grain directly determines that of this composite. Hence, this paper carries out experimental analysis on the Young’s moduli and parallel-to-grain tensile strengths of cornstalk scrimber and glue-laminated cornstalk scrimber. The results show that the load-strain curve of glue-laminated cornstalk scrimber basically changed linearly parallel to grain, and the material exhibited stable Young’s modulus and good strength; the glue-laminated cornstalk scrimber had a slightly higher tensile strength than cornstalk scrimber, and could thus replace timber as a building material.

scholarly journals PLATELET CONCENTRATES- PREPARATION PROTOCOLS AND RECENT ADVANCES

2020 ◽  
pp. 85-87
Author(s):  
Prathamesh Fulsundar ◽  
Vijaysinh More
Keyword(s):  
Tissue Repair ◽  
Autologous Blood ◽  
Effective Means ◽  
Cost Effective ◽  
Blood Platelet ◽  
Clinical Applications ◽  
Prolonged Release ◽  
Platelet Concentrates ◽  
Platelet Rich Fibrin ◽  
Tissue Healing

Autologous blood derivatives are surgical biologic additive that are prepared by manipulation of autologous blood. Platelet rich fibrin is one of the most commonly used blood derivatives in dentistry. Blood derivatives have several advantages such as being 100% autogenous, cost effective, less time consuming, simple to perform and with superior & prolonged release of growth factors. Since inception there has been evolution of various techniques, in-depth research regarding its biological actions, clinical applications. Several modifications have been advocated in the conventional protocol like the advanced PRF, injectable PRF, PRF lysate and Titanium-prepared PRF. Hence, the aim of this article is to review various types and properties of blood derivatives and the advancement in the PRF technology since its inception. Furthermore, platelet concentrates are safe, reliable and cost-effective means to accelerate tissue healing and for improving the efficiency of tissue repair after injury.

scholarly journals NaIO4/Br- as a mild system for the oxidation of 1-methyl-anthra-9,10-quinones

2015 ◽  
Author(s):  
Marcin Cybulski ◽  
Adam Formela ◽  
Katarzyna Sidoryk ◽  
Olga Michalak ◽  
Anna Rosa ◽  
...  
Keyword(s):  
High Pressure ◽  
Carboxylic Acids ◽  
Scale Up ◽  
Lithium Bromide ◽  
Cost Effective ◽  
Building Blocks ◽  
Multidrug Resistant ◽  
Resistant Cell ◽  
Direct Oxidation ◽  
Reaction Conditions

One of the anthraquinone classes comprises compounds with a carbonyl group. These natural or synthetic anthraquinones find their application as building blocks in the synthesis of the compounds with a biological activity. Recently, 4-substituted anthra-9,10-quinone-1-carboxylic acids (2) have been used as key intermediates in the synthesis of patented compounds (3) with anticancer activity against multidrug resistant cell lines. Although 2,7-dihydro-3H-dibenz[de,h]cinnolin-3,7-diones (3) were successfully synthetized in a small laboratory scale, several problems were observed during the preparation of their acid intermediates (2) in a multi-gram scale. The known methods for the preparation of 2 are based on the oxidation of the methyl group in anthra-9,10-quinones (1). The most common are: the oxidation with the diluted nitric acid under high pressure in a sealed tube at the temperature of 195-220 oC, the oxidation in nitrobenzene by passing chlorine gas through the reaction mixture at the temperature of 160-170 oC or in a presence of the fuming sulphuric acid. The mentioned methods require aggressive reagents and specific reaction conditions including high pressure and temperature. Thus, there was a need to find a new efficient, cost-effective and reproducible synthetic method of preparation of 2. While searching literature it was found that the direct oxidation of alkylarenes mediated by the sodium periodate/lithium bromide combination produces benzyl acetates throughout benzyl bromides in the acetic acid, or benzylic acids in the diluted inorganic acid. Based on these results we examined a variety of reaction conditions with or without the bromine source and the oxidizing anion. As a result, a novel procedure for the preparation of highly pure 4-substituted anthra-9,10-quinone-1-carboxylic acids (HPLC > 99.5%) using oxidizing anion/ brominating reagent system was developed. It enabled 2 isolation by the simple filtration of the reaction mixture and was applied in the scale-up of 2,7-dihydro-3H-dibenz[de,h]cinnolin-3,7-dione derivatives.

Effect of Mixing Quality on NOx Emissions in Reheat Combustion of GT24 and GT26 Engines

2011 ◽  
Author(s):  
K. Michael Du¨sing ◽  
Andrea Ciani ◽  
Adnan Eroglu
Keyword(s):  
High Pressure ◽  
Development Process ◽  
Flow Characteristic ◽  
Water Channel ◽  
Cost Effective ◽  
Nox Emissions ◽  
Combustion System ◽  
Low Pressure Turbine ◽  
Effective Development ◽  
System 1

Alstoms GT24 and GT26 engines feature a unique sequential combustion system [1, 2]. This system consists of a premixed combustor (called EV), which is followed by a high pressure turbine, a reheat combustor (called SEV) and a low pressure turbine (Figure 1). Recently improvements in NOx performance of the SEV have been demonstrated. Starting with relatively simple methods numerous design variants have been tested and down selected. Further down-selection has been done with methods of increased complexity. Overall a fast and cost effective development process has been assured. During the development process the variation coefficient and unmixedness measured and calculated for mixing only systems (CFD and water channel) has proven to be a reliable indicators for low NOx emissions for the real combustion system on atmospheric and high pressure test rigs. To demonstrate this a comparison of both quantities against NOx emissions is shown. The paper focuses on the NOx results achieved during this development and its relation to mixing quantities. Using this relation, together with a detailed understanding of the flow characteristic in the SEV burner, reductions in NOx emissions for GT24 and GT26 SEV burner and lance hardware can be reached using relatively simple methods.

Sensorless Detection of Cavitation in Centrifugal Pumps

2006 ◽  
Author(s):  
Parasuram P. Harihara ◽  
Alexander G. Parlos
Keyword(s):  
Mechanical Load ◽  
A Priori ◽  
Detection Threshold ◽  
Cost Effective ◽  
Detection Algorithm ◽  
Design Parameters ◽  
Centrifugal Pumps ◽  
Detection Scheme ◽  
Pump Design ◽  
Electric Power Supply

Analysis of electrical signatures has been in use for some time for estimating the condition of induction motors, by extracting spectral indicators from motor current waveforms. In most applications, motors are used to drive dynamic loads, such as pumps, fans, and blowers, by means of power transmission devices, such as belts, couplers, gear-boxes. Failure of either the electric motors or the driven loads is associated with operational disruption. The large costs associated with the resulting idle equipment and personnel can often be avoided if the degradation is detected in its early stages prior to reaching failure conditions. Hence the need arises for cost-effective detection schemes not only for assessing the condition of the motor but also of the driven load. This prompts one to consider approaches that use no add-on sensors, in order to avoid any reduction in overall system reliability and increased costs. This paper presents an experimentally demonstrated sensorless approach to detecting varying levels of cavitation in centrifugal pumps. The proposed approach is sensorless in the sense that no mechanical sensors are required on either the pump or the motor driving the pump. Rather, onset of pump cavitation is detected using only the line voltages and phase currents of the electric motor driving the pump. Moreover, most industrial motor switchgear are equipped with potential transformers and current transformers which can be used to measure the motor voltages and currents. The developed fault detection scheme is insensitive to electric power supply and mechanical load variations. Furthermore, it does not require a priori knowledge of a motor or pump model or any detailed motor or pump design parameters; a model of the system is adaptively estimated on-line. The developed detection algorithm has been tested on data collected from a centrifugal pump connected to a 3 φ, 3 hp induction motor. Several cavitation levels are staged with increased severity. In addition to these staged pump faults, extensive experiments are also conducted to test the false alarm performance of the algorithm. Results from these experiments allow us to offer the conclusion that for the cases under consideration, the proposed model-based detection scheme reveals cavitation detection times that are comparable to those obtained from vibration analysis with a detection threshold that is significantly lower than used in industrial practice.

Sign in / Sign up

Export Citation Format

Share Document