scholarly journals Macrophage-mediated angiogenic activation of outgrowth endothelial cells in co-culture with primary osteoblasts

2014 ◽  
Vol 27 ◽  
pp. 149-165 ◽  
Author(s):  
E Dohle ◽  
◽  
I Bischoff ◽  
T Böse ◽  
A Marsano ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Primary Osteoblasts ◽  
Angiogenic Activation

Lactoferrin inhibits the inflammatory and angiogenic activation of bovine aortic endothelial cells

2010 ◽  
Vol 60 (5) ◽  
pp. 475-482 ◽  
Author(s):  
Mijung Yeom ◽  
Jongbong Park ◽  
Bombi Lee ◽  
Sang-Yun Choi ◽  
Kyoung Soo Kim ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Aortic Endothelial Cells ◽  
Bovine Aortic Endothelial Cells ◽  
Angiogenic Activation ◽  
Aortic Endothelial

The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and endothelial cells

Cytotherapy ◽  
2011 ◽  
Vol 13 (8) ◽  
pp. 1000-1012 ◽  
Author(s):  
Wolfgang Metzger ◽  
Daniela Sossong ◽  
Annick Bächle ◽  
Norbert Pütz ◽  
Gunther Wennemuth ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Overlay Technique ◽  
Primary Osteoblasts

Angiogenic activation of valvular endothelial cells in aortic valve stenosis

2004 ◽  
Vol 298 (2) ◽  
pp. 455-464 ◽  
Author(s):  
Fariba Chalajour ◽  
Hendrik Treede ◽  
Alireza Ebrahimnejad ◽  
Heidrun Lauke ◽  
Hermann Reichenspurner ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Aortic Valve ◽  
Aortic Valve Stenosis ◽  
Angiogenic Activation

scholarly journals Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Wei Liu ◽  
Yanghui Ou ◽  
Yumeng Yang ◽  
Xuemei Zhang ◽  
Liqi Huang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Primary Cultures ◽  
Tube Formation ◽  
Endothelial Cell Proliferation ◽  
Adhesion Assay ◽  
Human Umbilical Vein ◽  
Tnf Α ◽  
Umbilical Vein Endothelial Cells ◽  
Angiogenic Activation

Punicalagin, a major ellagitannin isolated from pomegranate, is proved to have various pharmacological activities with an undefined therapy mechanism. The objective of this research was to demonstrate the effect of punicalagin on anti-inflammatory and angiogenic activation in human umbilical vein endothelial cells (HUVECs) and their potential mechanisms. Endothelial-leukocyte adhesion assay was applied to evaluate primary cultures of HUVECs activation following tumor necrosis factor alpha (TNF-α) treatment. The endothelial cell proliferation, migration, permeability and tube formation were assessed by EdU assay, wound migration assay, trans-endothelial electrical resistances (TEER) assay, and capillary-like tube formation assay, respectively. In addition, the expression of relevant proteins was assessed using Western blot analysis. We confirmed that punicalagin could reduce the adhesion of human monocyte cells to HUVECs in vitro and in vivo. Further, punicalagin decreased the expression of mRNA and proteins of ICAM-1 and VCAM-1 in HUVECs. Moreover, punicalagin inhibited permeability, proliferation, migration, and tube formation in VEGF-induced HUVECs, suppressed IKK-mediated activation of NF-κB signaling in TNF-α-induced endothelial cells, and inhibited vascular endothelial growth factor receptor 2 (VEGFR2) activation and downstream p-PAK1. Our findings indicated that punicalagin might have a protective effect on HUVECs activation, which suggested that punicalagin functions through an endothelial mediated mechanism for treating various disorders such as, cancer, rheumatoid arthritis, and cardiovascular disease.

Effects of endothelial cells on proliferation and survival of human mesenchymal stem cells and primary osteoblasts

2012 ◽  
Vol 30 (10) ◽  
pp. 1682-1689 ◽  
Author(s):  
Dominik Steiner ◽  
Florian Lampert ◽  
G. Björn Stark ◽  
Günter Finkenzeller
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Primary Osteoblasts

scholarly journals Cell Communication in a Coculture System Consisting of Outgrowth Endothelial Cells and Primary Osteoblasts

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
David Paul Eric Herzog ◽  
Eva Dohle ◽  
Iris Bischoff ◽  
Charles James Kirkpatrick
Keyword(s):  
Endothelial Cells ◽  
Growth Factors ◽  
Gap Junctions ◽  
Bone Tissue ◽  
Bone Repair ◽  
Cell Communication ◽  
Signal Molecules ◽  
Cell Nuclei ◽  
Repair Processes ◽  
Primary Osteoblasts

Bone tissue is a highly vascularized and dynamic system with a complex construction. In order to develop a construct for implant purposes in bone tissue engineering, a proper understanding of the complex dependencies between different cells and cell types would provide further insight into the highly regulated processes during bone repair, namely, angiogenesis and osteogenesis, and might result in sufficiently equipped constructs to be beneficial to patients and thereby accomplish their task. This study is based on anin vitrococulture model consisting of outgrowth endothelial cells and primary osteoblasts and is currently being used in different studies of bone repair processes with special regard to angiogenesis and osteogenesis. Coculture systems of OECs and pOBs positively influence the angiogenic potential of endothelial cells by inducing the formation of angiogenic structures in long-term cultures. Although many studies have focused on cell communication, there are still numerous aspects which remain poorly understood. Therefore, the aim of this study is to investigate certain growth factors and cell communication molecules that are important during bone repair processes. Selected growth factors like VEGF, angiopoietins, BMPs, and IGFs were investigated during angiogenesis and osteogenesis and their expression in the cultures was observed and compared after one and four weeks of cultivation. In addition, to gain a better understanding on the origin of different growth factors, both direct and indirect coculture strategies were employed. Another important focus of this study was to investigate the role of “gap junctions,” small protein pores which connect adjacent cells. With these bridges cells are able to exchange signal molecules, growth factors, and other important mediators. It could be shown that connexins, the gap junction proteins, were located around cell nuclei, where they await their transport to the cell membrane. In addition, areas in which two cells formed gap junctions were found.

Mo2070 Modulatory Effect of Hypoxia on Inflammatory and Angiogenic Activation of Human Intestinal Microvascular Endothelial Cells

2012 ◽  
Vol 142 (5) ◽  
pp. S-733
Author(s):  
Annette Wilson ◽  
William M. Rivers ◽  
Anthony J. Bauer ◽  
Arthur Barrie ◽  
David G. Binion
Keyword(s):  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial ◽  
Angiogenic Activation
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