scholarly journals The use of non-viral gene vectors for bioactive poly-(D,L-lactide) implant surfaces in bone tissue engineering

2012 ◽  
Vol 23 ◽  
pp. 441-448 ◽  
Author(s):  
AK Reckhenrich ◽  
◽  
C Koch ◽  
JT Egaña ◽  
C Plank
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Viral Gene ◽  
Gene Vectors

scholarly journals Dual non-viral gene delivery from microparticles within 3D high-density stem cell constructs for enhanced bone tissue engineering

Biomaterials ◽  
2018 ◽  
Vol 161 ◽  
pp. 240-255 ◽  
Author(s):  
Alexandra McMillan ◽  
Minh Khanh Nguyen ◽  
Tomas Gonzalez-Fernandez ◽  
Peilin Ge ◽  
Xiaohua Yu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Gene Delivery ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
High Density ◽  
Viral Gene ◽  
Viral Gene Delivery

scholarly journals Non-viral gene therapy for bone tissue engineering

2013 ◽  
Vol 29 (2) ◽  
pp. 206-220 ◽  
Author(s):  
Fiona Wegman ◽  
F. Cumhur Öner ◽  
Wouter J.A. Dhert ◽  
Jacqueline Alblas
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Viral Gene ◽  
Viral Gene Therapy

Porous bioprinted constructs in BMP-2 non-viral gene therapy for bone tissue engineering

2013 ◽  
Vol 1 (48) ◽  
pp. 6619 ◽  
Author(s):  
Loek D. Loozen ◽  
Fiona Wegman ◽  
F. Cumhur Öner ◽  
Wouter J. A. Dhert ◽  
Jacqueline Alblas
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Viral Gene ◽  
Viral Gene Therapy

Non-Viral Gene Delivery to Mesenchymal Stem Cells: Methods, Strategies and Application in Bone Tissue Engineering and Regeneration

2011 ◽  
Vol 11 (1) ◽  
pp. 46-57 ◽  
Author(s):  
Jose L. Santos ◽  
Deepti Pandita ◽  
Joao Rodrigues ◽  
Ana P. Pego ◽  
Pedro L. Granja ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Viral Gene ◽  
Viral Gene Delivery

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

PLGA+HA/βTCP scaffold incorporating simvastatin: a promising biomaterial for bone tissue engineering

2020 ◽  
Author(s):  
Mariane Beatriz Sordi ◽  
Ariadne Cristiane Cabral da Cruz ◽  
Águedo Aragones ◽  
Mabel Mariela Rodríguez Cordeiro ◽  
Ricardo de Souza Magini
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Thermal Analyses ◽  
Chemical Properties ◽  
Biological Properties ◽  
Scanning Calorimetry ◽  
Evaporation Technique ◽  
Porosity Analysis ◽  
Biphasic Ceramic

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite / β-tricalcium phosphate (PLGA+HA/βTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% βTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m:m) of this medication. Scaffolds were synthesized in a cylindric-shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in PBS at 37 °C under constant stirring for 7, 14, 21, and 28 days. Non-degraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro, meso, and micropores in the polymer structure with HA/βTCP particles homogeneously dispersed. Chemical and thermal analyses presented very similar results for both PLGA+HA/βTCP and PLGA+HA/βTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/βTCP and PLGA+HA/βTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/βTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.

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