scholarly journals Biodegradation of porous calcium phosphate scaffolds in an ectopic bone formation model studied by X-ray computed microtomograph

2010 ◽  
Vol 19 ◽  
pp. 136-146 ◽  
Author(s):  
VS Komlev ◽  
◽  
M Mastrogiacomo ◽  
RC Pereira ◽  
F Peyrin ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Ectopic Bone Formation ◽  
Formation Model ◽  
X Ray ◽  
Ectopic Bone ◽  
X Ray Computed

Ectopic Bone Formation in Titanium Mesh Loaded with Bone Morphogenetic Protein and Coated with Calcium Phosphate

2001 ◽  
Vol 108 (2) ◽  
pp. 434-443 ◽  
Author(s):  
Johan W. M. Vehof ◽  
Javed Mahmood ◽  
Hiroko Takita ◽  
Martin A. vanʼt Hof ◽  
Yoshinori Kuboki ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Ectopic Bone Formation ◽  
Titanium Mesh ◽  
Morphogenetic Protein ◽  
Ectopic Bone

scholarly journals Coralline Hydroxyapatite Coated with a Layer Biomimetic Calcium Phosphate Containing BMP-2 Induces Dose-Related Ectopic Bone Formation in Wistar Rats

Coatings ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1195
Author(s):  
Henri J. J. Uijlenbroek ◽  
Xingnan Lin ◽  
Xin Zhang ◽  
Liquan Deng ◽  
Daniel Wismeijer ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Wistar Rats ◽  
Ectopic Bone Formation ◽  
Bone Morphogenetic Protein 2 ◽  
Ectopic Bone ◽  
Coralline Hydroxyapatite ◽  
Male Wistar Rats ◽  
Loading Methods ◽  
Histological Results

In order to evaluate loading methods and the dose dependency of bone morphogenetic protein 2 (BMP-2) in ectopic bone formation, an osteoinductive material consisting of commercially available coralline hydroxyapatite (CHA) was coated with a layer of biomimetic calcium phosphate (BioCaP) containing BMP-2 in different ways. Eight groups—each containing samples of 0.25 g CHA—were formed and coated with, respectively, BioCaP with internally incorporated BMP-2 in concentrations of 1, 5, 10, 20, 40 and 60 µg per sample, and the two control groups with BioCaP only and BioCaP with 20 µg of adsorbed BMP-2 per sample. The samples were implanted subcutaneously in 27 male Wistar rats. The histological results show that there is no bone formation in the group in which no BMP-2 was included. All samples with BioCaP containing BMP-2 show bone formation. The group with 20 µg of adsorbed BMP-2 per sample shows the least bone formation. Coating-incorporated BMP-2 is more efficient in inducing bone formation than adsorbed BMP-2. The group with 5 µg of coating-incorporated BMP-2 per sample shows the most bone formation. Increasing the amount of coating-incorporated BMP-2 up to 60 µg does not improve ectopic bone formation.

Ectopic bone formation by microporous calcium phosphate ceramic particles in sheep muscles

Bone ◽  
2005 ◽  
Vol 36 (6) ◽  
pp. 1086-1093 ◽  
Author(s):  
D LENIHOUANNEN ◽  
G DACULSI ◽  
A SAFFARZADEH ◽  
O GAUTHIER ◽  
S DELPLACE ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Ectopic Bone Formation ◽  
Calcium Phosphate Ceramic ◽  
Ceramic Particles ◽  
Ectopic Bone

Exercise enhance the ectopic bone formation of calcium phosphate biomaterials in muscles of mice

2017 ◽  
Vol 77 ◽  
pp. 136-141 ◽  
Author(s):  
Lijia Cheng ◽  
Shuo Yan ◽  
Jiang Zhu ◽  
Peiling Cai ◽  
Ting Wang ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Ectopic Bone Formation ◽  
Ectopic Bone

scholarly journals Treatment of collagenase-induced osteoarthritis with a viral vector encoding TSG-6 results in ectopic bone formation

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4771 ◽  
Author(s):  
Mathijs G.A. Broeren ◽  
Irene Di Ceglie ◽  
Miranda B. Bennink ◽  
Peter L.E.M. van Lent ◽  
Wim B. van den Berg ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Bone Formation ◽  
Protease Activity ◽  
Expression Vector ◽  
Cartilage Damage ◽  
Treated Group ◽  
Ectopic Bone Formation ◽  
X Ray ◽  
Ectopic Bone ◽  
Experimental Osteoarthritis

Objective Tumor necrosis factor-inducible gene 6 (TSG-6) has anti-inflammatory and chondroprotective effects in mouse models of inflammatory arthritis. Because cartilage damage and inflammation are also observed in osteoarthritis (OA), we determined the effect of viral overexpression of TSG-6 in experimental osteoarthritis. Methods Bone marrow-derived cells were differentiated to multinucleated osteoclasts in the presence of recombinant TSG-6 or after transduction with a lentiviral TSG-6 expression vector. Multi-nucleated osteoclasts were analyzed after tartrate resistant acid phosphatase staining and resorption activity was determined on dentin slices. Collagenase-induced osteoarthritis (CIOA) was induced in C57BL/6 mice after intra-articular injection of an adenoviral TSG-6 or control luciferase expression vector. Inflammation-related protease activity was measured using bioluminescent Prosense probes. After a second adenovirus injection, cartilage damage was assessed in histological sections stained with Safranin-O. Ectopic bone formation was scored in X-ray images of the affected knees. Results TSG-6 did not inhibit the formation of multi-nucleated osteoclasts, but caused a significant reduction in the resorption activity on dentin slices. Adenoviral TSG-6 gene therapy in CIOA could not reduce the cartilage damage compared to the luciferase control virus and no significant difference in inflammation-related protease activity was noted between the TSG-6 and control treated group. Instead, X-ray analysis and histological analysis revealed the presence of ectopic bone formation in the TSG-6 treated group. Conclusion Gene therapy based on the expression of TSG-6 could not provide cartilage protection in experimental osteoarthritis, but instead resulted in increased ectopic bone formation.

The role of bFGF on the ability of MSC to activate endogenous regenerative mechanisms in an ectopic bone formation model

Biomaterials ◽  
2012 ◽  
Vol 33 (7) ◽  
pp. 2086-2096 ◽  
Author(s):  
Roberta Tasso ◽  
Massimiliano Gaetani ◽  
Erica Molino ◽  
Angela Cattaneo ◽  
Massimiliano Monticone ◽  
...  
Keyword(s):  
Bone Formation ◽  
Ectopic Bone Formation ◽  
Formation Model ◽  
Ectopic Bone
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