scholarly journals Fabrication of highly porous scaffold materials based on functionalized oligolactides and preliminary results on their use in bone tissue engineering

2002 ◽  
Vol 4 ◽  
pp. 30-38 ◽  
Author(s):  
S Vogt ◽  
◽  
Y Larcher ◽  
B Beer ◽  
I Wilke ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
Preliminary Results ◽  
Scaffold Materials ◽  
Highly Porous

scholarly journals Highly porous scaffolds of PEDOT:PSS for bone tissue engineering

2017 ◽  
Vol 62 ◽  
pp. 91-101 ◽  
Author(s):  
Anne Géraldine Guex ◽  
Jennifer L. Puetzer ◽  
Astrid Armgarth ◽  
Elena Littmann ◽  
Eleni Stavrinidou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffolds ◽  
Highly Porous

scholarly journals Highly porous PHB-based bioactive scaffolds for bone tissue engineering by in situ synthesis of hydroxyapatite

2019 ◽  
Vol 100 ◽  
pp. 286-296 ◽  
Author(s):  
Micaela Degli Esposti ◽  
Federica Chiellini ◽  
Federica Bondioli ◽  
Davide Morselli ◽  
Paola Fabbri
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
In Situ Synthesis ◽  
Bioactive Scaffolds ◽  
Highly Porous

scholarly journals Poly(d,l-Lactic acid) Composite Foams Containing Phosphate Glass Particles Produced via Solid-State Foaming Using CO2 for Bone Tissue Engineering Applications

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 231 ◽  
Author(s):  
Maziar Shah Mohammadi ◽  
Ehsan Rezabeigi ◽  
Jason Bertram ◽  
Benedetto Marelli ◽  
Richard Gendron ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Solid State ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Melt Compounding ◽  
Composite Foams ◽  
Biodegradable Poly ◽  
Gaseous Carbon Dioxide ◽  
Highly Porous

This study reports on the production and characterization of highly porous (up to 91%) composite foams for potential bone tissue engineering (BTE) applications. A calcium phosphate-based glass particulate (PGP) filler of the formulation 50P2O5-40CaO-10TiO2 mol.%, was incorporated into biodegradable poly(d,l-lactic acid) (PDLLA) at 5, 10, 20, and 30 vol.%. The composites were fabricated by melt compounding (extrusion) and compression molding, and converted into porous structures through solid-state foaming (SSF) using high-pressure gaseous carbon dioxide. The morphological and mechanical properties of neat PDLLA and composites in both nonporous and porous states were examined. Scanning electron microscopy micrographs showed that the PGPs were well dispersed throughout the matrices. The highly porous composite systems exhibited improved compressive strength and Young’s modulus (up to >2-fold) and well-interconnected macropores (up to ~78% open pores at 30 vol.% PGP) compared to those of the neat PDLLA foam. The pore size of the composite foams decreased with increasing PGPs content from an average of 920 µm for neat PDLLA foam to 190 µm for PDLLA-30PGP. Furthermore, the experimental data was in line with the Gibson and Ashby model, and effective microstructural changes were confirmed to occur upon 30 vol.% PGP incorporation. Interestingly, the SSF technique allowed for a high incorporation of bioactive particles (up to 30 vol.%—equivalent to ~46 wt.%) while maintaining the morphological and mechanical criteria required for BTE scaffolds. Based on the results, the SSF technique can offer more advantages and flexibility for designing composite foams with tunable characteristics compared to other methods used for the fabrication of BTE scaffolds.

Preparation and Characterization of Fibroin/Chitosan/Hydroxyapatite Porous Scaffold

2013 ◽  
Vol 849 ◽  
pp. 151-156 ◽  
Author(s):  
Tarin Sukhachiradet ◽  
Wassanai Wattanutchariya
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
Chemical Precipitation ◽  
Precipitation Method ◽  
Xtt Assay ◽  
Average Porosity ◽  
Biocompatibility Test

Autograft is a general method used in orthopedic surgery for a bone replacement. However, the disadvantage of this method is the amount of risk factor to the donor sites. Currently, bone tissue engineering is another technique that could be implemented to solve this problem. Artificial bone scaffold generated by bone tissue engineering can be employed in order to accelerate damaged bone regeneration. In fact, this scaffold can be fabricated from synthetic contents such as bioceramics, biopolymers or composite. Three types of biomaterials: Chitosan, Hydroxyapatite (HA) and Fibroin were used to form porous scaffold. This research investigated the preparation of Hydroxyapatite and Fibroin from natural materials. Hydroxyapatite was synthesized from mollusk shell by wet chemical precipitation method. While, Fibroin was extracted from silk worms cocoons. Freeze drying method was employed to fabricate this composite porous scaffold. A mixing ratio of 1:2:1 among Fibroin: Chitosan: HA was studied to evaluate biodegradability, biocompatibility, porosity and pore structure of the output scaffolds. Results show that the output scaffolds have an interconnected porous structure with a pore size around 150-200μm and an average porosity of 94.26%. While the average degradation rate of the scaffold in lysozyme was 10.46% at 7 days. In addition, the biocompatibility test based on XTT assay test, shown that the scaffolds were non-cytotoxicity, which could be good for bone filling application in the future.

Fabrication of a RP-Based Biomimetic Grafting Material for Bone Tissue Engineering

2009 ◽  
Vol 626-627 ◽  
pp. 553-558 ◽  
Author(s):  
Xing Ma ◽  
Y.Y. Hu ◽  
Xiao Ming Wu ◽  
J. Liu ◽  
Zhuo Xiong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Collagen Type ◽  
Autologous Bone Marrow ◽  
Collagen Type I ◽  
Control Group ◽  
Type I ◽  
High Porosity ◽  
Highly Porous

Three-dimensional (3D) highly porous poly (DL-lactic-co-glycolic acid)/tricalcium phosphate (PLGA/TCP) scaffolds were fabricated using a rapid prototyping technique (RP). The biopolymer carriers (4mm×4mm×4mm) subsequently were coated with collagen type I (Col) to produce PLGA/TCP/Col composites and utilized as an extracellular matrix for a cell-based strategy of bone tissue engineering. Autologous bone marrow stromal cells (BMSCs) harvested from New Zealand white rabbits were cultured under an osteogenic condition (BMSCs-OB) followed by seeding into the structural highly porous PLGA/TCP/Col composites (i.e. PLGA/TCP/Col/BMSCs-OB). Scanning electron microscopy observation found that the RP-based scaffolds had appropriate microstructure, controlled interconnectivity and high porosity. Modification of the scaffolds with collagen type I (PLGA/TCP/Col) essentially increased the affinity of the carriers to seeding cells, and PLGA/TCP/Col composites were well biocompatible with BMSCs-OB. The PLGA/TCP/Col/BMSCs-OB constructs were then subcutaneously implanted in the back of rabbits compared to controls with autologous BMSCs suspension and carriers alone. As a result, histological new bone formation was observed only in the experimental group with PLGA/TCP/Col/BMSCs-OB constructs 8 weeks after implantation. In the control group with scaffold alone only biodegradation of the carriers was found. Therefore, these results validate our bio-manufacturing methods for a new bone graft substitute.

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