scholarly journals Possible Role of Myoglobin in Regulating Calpain-1 Activity in Postmortem Beef Muscle

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
J. V. Cooper ◽  
S. Suman ◽  
Z. D. Callahan ◽  
K. C. Kerns ◽  
M. Zigo ◽  
...  
Keyword(s):  
Shear Force ◽  
Beef Tenderness ◽  
Beef Carcasses ◽  
Muscle Ph ◽  
Reducing Activity ◽  
The Right ◽  
Beef Color ◽  
Moderate Relationship ◽  
Redox Forms

ObjectivesPrevious research revealed a relationship between meat color and beef tenderness and indicated that myoglobin can inhibit calpain-1 in solution. The objective of this study was to determine the extent to which myoglobin and beef color are associated with calpain activity and beef tenderness.Materials and MethodsBeef Longissimus dorsi samples from the left side of Holstein beef carcasses (n = 21) were collected immediately post exsanguination on the processing floor for 0 h analyses. Muscle temperature and pH was measured at 0, 24, and 48 h postmortem. After USDA quality and yield grade determination, steaks (n = 6) were removed from the right side of each carcass (n = 21) at 48 h for analyses at 48 and 336 h postmortem. Color (L*, a*, and b* values), surface myoglobin redox forms, metmyoglobin reducing activity (MRA), total myoglobin concentrations, slice shear force (SSF), Warner-Bratzler shear force (WBSF) were measured. Calpain-1 concentrations and autolysis were determined via Western blot at 0, 48, and 336 h.ResultsDecline in muscle pH was 6.4, 5.8, and 5.6 at 0, 24, and 48 h, respectively. Shear force values at 48 h were 73.19 N for WBSF and 384.21 N for SSF and at 336 h were 48.75 N for WBSF and 260.47 N for SSF. Myoglobin reducing activity at 336 h was positively correlated to WBSF at 48 h and negatively correlated to calpain-1 concentration at 0 h (P < 0.05; Table 9). Color measurements of L* and b* at 48 h were moderately correlated with WBSF at 336 h (P < 0.05; Table 9). The b* measurement at 336 h showed a moderate relationship to calpain-1 concentration at 0 h (P < 0.05; Table 9).ConclusionModerate correlations between color and tenderness measurements taken at 48 h with those taken at 336 h were discovered indicating that myoglobin may impact calpain-1 in vivo.Table 9Correlations (P-values) between selected color and tenderness measurements (n = 21)

scholarly journals Electrical stimulator voltage settings minimally influences beef longissimus muscle tenderness

2021 ◽  
Author(s):  
Travis S Arp ◽  
Emily Rice ◽  
Dale R Woerner ◽  
Kenichi Kathoh ◽  
Gary Smith ◽  
...  
Keyword(s):  
Shear Force ◽  
Low Voltage ◽  
Carcass Quality ◽  
Longissimus Muscle ◽  
Beef Processing ◽  
Beef Carcasses ◽  
Muscle Tenderness ◽  
Muscle Ph ◽  
Processing Plants ◽  
Electrical Stimulator

The objective of the current study was to evaluate the effect of differing electrical stimulation (ES) voltage levels on beef longissimus muscle (LM) tenderness, postmortem temperature, and pH decline, and carcass quality. Beef carcasses from three commercial beef processing plants (A, B, C) were exposed to three varying voltage levels: 1) control (no ES) 2) high ES (60-Hz for 17s each at 25, 35, 45, and 55 V) 3) low-ES (60-Hz for 17s each at 16, 20, 24, and 28 V). Ninety beef carcasses were selected from the three plants, and within a carcass, paired sides were randomly assigned to one of three ES treatments. The results indicated that ES affected (P &lt; 0.05) muscle pH at 3 h postmortem in two of the three plants. However, ES did not affect (P &gt; 0.05) pH at the time of grading (post rigor). Although the slice shear force (SSF) values were lower (P &lt; 0.05) for ES steaks compared to controls, it was not (P &lt; 0.05) influenced by the voltage levels. Variation in tenderness was observed among the plants (P &lt; 0.05), with Plant C having the toughest steaks, whereas Plant A and B exhibited similar (P &gt; 0.05) tenderness. Overall, the lack of difference in postmortem tenderness between high- and low-voltage settings indicated the ES-voltage minimally influenced tenderness.&nbsp;

scholarly journals Visual evaluation of beef tenderness by using surface structural observations and its relationship to meat colour

1970 ◽  
Vol 45 (3) ◽  
pp. 255-262
Author(s):  
KY Modika ◽  
L Frylinck ◽  
KW Moloto ◽  
PE Strydom ◽  
PH Heinze ◽  
...  
Keyword(s):  
Shear Force ◽  
Visual Analysis ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Post Mortem ◽  
Visual Evaluation ◽  
Beef Tenderness ◽  
Meat Colour ◽  
The Right ◽  
Visual Colour

The study describes the relationship between visual and instrumental measurements for colour and tenderness between five South African beef breeds: Bos indicus (Brahman), Sanga type (Nguni), British Bos taurus (Angus), European Bos taurus (Charolais) and the composite (Bonsmara). Ten animals per genotype were used (total = 50). The carcasses were split and the right sides were electrically stimulated, while the left sides were not stimulated. Steaks were aged until three days post mortem on polystyrene plates and until 9, 14 and 20 days post mortem in vacuum bags. The steaks were evaluated by visual analysis for colour, marbling, fibre separation, surface texture and structure integrity by a 10-member trained panel. Colour was also measured by the CIE L*, a*, b* system using a Minolta meter, and tenderness was measured by means of Warner-Bratzler shear force. High negative correlations were observed between the visual colour and L* (r = −0.809), b* (r = −0.698) and high positive correlations were observed between the visual colour and hue (r = 0.797). There were also negative correlations between shear force and structure integrity (r = −0.410) and fibre separation (r = −0.401). Very low negative correlations were observed between colour and shear force (r = −0.242). Therefore, although it may be possible to judge meat colour by visual analysis, it does not appear possible to predict tenderness by colour judgment. There is potential for an experienced eye to predict tenderness by observing visual structural properties such as fibre separation and structural integrity.Keywords: Meat colour and tenderness, tenderness prediction, trained visual panel, visual analysis

VARIATION IN MEAT QUALITY AT THREE SITES ALONG THE LENGTH OF THE BEEF LONGISSIMUS MUSCLE

1990 ◽  
Vol 70 (2) ◽  
pp. 707-710 ◽  
Author(s):  
C. GARIÉPY ◽  
S. D. M. JONES ◽  
W. M. ROBERTSON
Keyword(s):  
Key Words ◽  
Meat Quality ◽  
Shear Force ◽  
Lumbar Vertebra ◽  
Quality Parameters ◽  
Drip Loss ◽  
Longissimus Muscle ◽  
Muscle Ph ◽  
The Right ◽  
Quality Variation

Variation in meat quality parameters (pH, color, shear force, expressible juice, drip loss) for the longissimus muscle at three sites (site 1 = 4–7th ribs, site 2 = 8–12th ribs, site 3 = 13th rib - 5th lumbar vertebra) was investigated in the right carcass sides from 80 steers. Muscle pH was similar for all three sites at 45 min and at 24 h postmortem. At 6 d, muscle pH was higher (P < 0.05) at site 1 than site 3. Visually assessed color scores were similar (P > 0.05) for all three sites. Minor differences were found for expressible juice and drip loss across the three sites. It was concluded that apart from shear force, quality variation at the three sites along the longissimus muscle was minor. Key words: Beef, longissimus, meat quality variation

The role of the right inferior frontal gyrus following reversal of consistent stimulus-stop mappings

2013 ◽  
Author(s):  
Maisy Best ◽  
Tobias Stevens ◽  
Fraser Milton ◽  
Christopher D. Chambers ◽  
Ian P. McLaren ◽  
...  
Keyword(s):  
Inferior Frontal Gyrus ◽  
The Right

Managing Landscape Irrigation to Avoid Soil and Nutrient Losses

EDIS ◽  
2013 ◽  
Vol 2013 (11) ◽  
Author(s):  
George Hochmuth ◽  
Laurie Trenholm ◽  
Don Rainey ◽  
Esen Momol ◽  
Claire Lewis ◽  
...  
Keyword(s):  
Natural Environment ◽  
Management Practices ◽  
Root Zone ◽  
Irrigation Management ◽  
Critical Factor ◽  
Nutrient Losses ◽  
County Agents ◽  
The Right ◽  
Soil And Water

Proper irrigation management is critical to conserve and protect water resources and to properly manage nutrients in the home landscape. How lawns and landscapes are irrigated directly impacts the natural environment, so landscape maintenance professionals and homeowners must adopt environmentally-friendly approaches to irrigation management. After selecting the right plant for the right place, water is the next critical factor to establish and maintain a healthy lawn and landscape. Fertilization is another important component of lawn and landscape maintenance, and irrigation must be applied correctly, especially following fertilization, to minimize potential nutrient losses. This publication supplements other UF/IFAS Extension publications that also include information on the role of soil and the root zone in irrigation management. This publication is designed to help UF/IFAS Extension county agents prepare materials to directly address nutrient losses from lawns and landscapes caused by inadequate irrigation management practices. This 6-page fact sheet was written by George Hochmuth, Laurie Trenholm, Don Rainey, Esen Momol, Claire Lewis, and Brian Niemann, and published by the UF Department of Soil and Water Science, October 2013. http://edis.ifas.ufl.edu/ss586

Formation of financial literacy in training of informatics teachers on the basis of the 1С:Enterprise system

2020 ◽  
Vol 1 (1) ◽  
pp. 11-18
Author(s):  
M. A. Rodionov ◽  
I. V. Akimova
Keyword(s):  
Financial Literacy ◽  
Basic Knowledge ◽  
State University ◽  
Science And Literature ◽  
Successful Development ◽  
Mathematics History ◽  
School Subjects ◽  
Personal Finances ◽  
The Right

In the submitted study the problem of the formation of financial literacy of students at informatics lessons and relevant training of future informatics teachers is considered. Financial literacy is understood as a set of basic knowledge in the field of finance, banking, insurance, as well as budgeting for personal finances that allow a person to choose the right financial product or service, soberly assess and take risks that may arise during the use of these products, correctly accumulate savings and identify doubtful (fraudulent) investment schemes. The authors conclude that successful development of meaningful lines of the course of financial literacy requires integration of a few school subjects, such as mathematics, history, informatics, social science and literature. The role of modern informatics teacher in the formation of financial literacy of students is great. Therefore, in the training of a future informatics teacher, it should be paid the attention to issues related to the study of elements of financial literacy in informatics lessons. In order to solve the problem, the authors propose to use the special course “Basics of work in 1С:Enterprise”, which is implemented at Penza State University. The article contains a program of the course and the methodological recommendations for its implementation.

The adaptation of design thinking in auditing

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Linda MEIJER-WASSENAAR ◽  
Diny VAN EST
Keyword(s):  
The Netherlands ◽  
Design Thinking ◽  
Public Organizations ◽  
Design Framework ◽  
The Right ◽  
The Way

How can a supreme audit institution (SAI) use design thinking in auditing? SAIs audit the way taxpayers’ money is collected and spent. Adding design thinking to their activities is not to be taken lightly. SAIs independently check whether public organizations have done the right things in the right way, but the organizations might not be willing to act upon a SAI’s recommendations. Can you imagine the role of design in audits? In this paper we share our experiences of some design approaches in the work of one SAI: the Netherlands Court of Audit (NCA). Design thinking needs to be adapted (Dorst, 2015a) before it can be used by SAIs such as the NCA in order to reflect their independent, autonomous status. To dive deeper into design thinking, Buchanan’s design framework (2015) and different ways of reasoning (Dorst, 2015b) are used to explore how design thinking can be adapted for audits.

ANDROST-5-ENE-3β,17β-DIOL IN OVARY OF POST-MENOPAUSAL HYPEROESTROGENIC WOMEN

1968 ◽  
Vol 58 (3) ◽  
pp. 364-376 ◽  
Author(s):  
S. Pesonen ◽  
M. Ikonen ◽  
B-J. Procopé ◽  
A. Saure
Keyword(s):  
Ovarian Tissue ◽  
Cortical Layer ◽  
Vaginal Smears ◽  
Incubation Experiments ◽  
Cell Groups ◽  
Post Menopause ◽  
Reducing Activity ◽  
Post Menopausal ◽  
One Year

ABSTRACT The ovaries of ten patients, at least one year after the post-menopause, were incubated with two Δ5-C19-steroids and also studied histochemically. All these patients had post-menopausal uterine bleeding and increased oestrogen excretion of the urine. The urinary estimations of gonadotrophins, 17-KS, 17-OHCS and pregnanediol were carried out on all patients. Vaginal smears were read according to Papanicolaou, and the endometrium and ovaries were studied histologically. The incubation experiments indicate the presence of Δ5-3β-hydroxysteroid-dehydrogenase. When androst-5-ene-3β,17β-diol was used as precursor the formation of testosterone occurred without any concomitant production of DHA and/or androstenedione. This seems to indicate the possible role of the Δ5-pathway in the formation of testosterone by post-menopausal ovarian tissue. The histochemical reactions indicated a reducing activity on NADH, lactate and glucose-6-phosphate, in certain corpora albicantia, atretic follicles and in diffuse thecoma regions in the cortical layer of the ovary. Steroid-3β-ol-dehydrogenase and β-hydroxybutyrate-dehydrogenase were found only at the edges of certain corpora albicantia, in some individual stroma cell groups and in some atretic follicles. Our studies, both biochemical and histochemical, suggest that the observed increase in the urinary oestrogens of the patients studied might in part at least, be of ovarian origin. This opinion is also supported by the postoperative oestrogen values.

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