scholarly journals Evaluation of Consumer Preferences and Volatile Compounds of Beef Strip Loin Steaks Differing by Quality Grade, Postmortem Aging, and Degree of Doneness

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
D. H. Tucker ◽  
C. R. Kerth ◽  
K. R. Wall ◽  
Z. M. Hicks ◽  
R. K. Miller

ObjectivesThe purpose of this study was to determine consumer preferences and volatile aroma compounds for differences in flavor concerning quality grade, day of age, and degree of doneness on beef strip loins.Materials and MethodsUSDA Select (n = 18) and USDA upper 2/3 Choice (n = 18), boneless beef strip loins (IMPS 180), were selected from a commercial processing plant. Loins were cut in half and wet aged for either 10 or 20 d at 2°C. After aging, loins were cut into 2.54 cm steaks, individually vacuum-packaged and stored in a freezer at –40°C. Steaks were thawed at 4°C for 12 to 24 h prior to cooking. Steaks were cooked on a flat top griddle set to 204.4°C (± 11.1°C). The steaks were cooked to one of three degrees of doneness: 63°C (63; medium rare), 71°C (71; medium) or 80°C (80; medium well) and flipped once at the halfway cook temperature. Steaks were held at 60°C no longer than 20 min. Consumer testing was conducted over five sessions with 93 consumers. Each consumer evaluated the samples on five different attributes: overall liking, overall flavor, appearance juiciness, and tenderness. The consumers rated each sample based on a 9-point hedonic scale. Consumer data were run using a full factorial design using grade, age, and degree of doneness as main effects. The order in which samples were served was included as a random effect and data were blocked by session. Portions of cooked samples were collected for GC analysis by being placed into a 20mL glass jar and collected with a solid-phase micro-extraction fiber for 60 min. The SPME was then placed into a GC/MS to separate and identify each volatile chemical compound. Three-way interactions among volatile compounds were determined to be not significant (P > 0.05); therefore, they were removed from the model. Additionally, volatiles that were not present in cells of two-way interactions were not included. Multivariate relationships between consumer preference and GC/MS data were explored using PCA.ResultsUSDA Choice had a higher (P < 0.001) liking score than USDA Select grade beef loins for each of the five attributes tested. The 20-d aged steaks had higher (P < 0.03) scores for overall liking, overall flavor, juiciness, and tenderness. The degree of doneness affected overall liking and juiciness liking (P < 0.001) with 63°C having the greatest score followed by 71°C and then 80°C. For overall flavor, 63°C and 71°C were greater (P = 0.013) than for 80°C. For appearance, the degree of doneness of 63°C was preferred to steaks cooked at 71°C and 80°C (P = 0.002). Of the total volatiles (n = 52) present in the samples, 20 d age had greater (P < 0.04) iso butyraldehyde (pungent), 2-methyl-butanal (chocolate), and 3-methyl-butanal (fatty almond). Whereas, 3-hydroxy-2-butanone (buttery) was greater (P < 0.002) in 10 d age. Octanal (fatty) and nonanal (fatty) were greater (P < 0.04) in USDA Select than USDA Choice. 2-methyl pyrazine (chocolate, meaty, roasted) was greater (P < 0.04) in 20 d aged steaks cooked to 71°C and 80°C compared to other treatment combinations.ConclusionConsumer preferences were distinctly different based on quality grade, age, and degree of doneness. USDA Choice was generally the most preferred along with 63°C and 20 d age steaks. Positive (by their descriptors) volatile aroma compounds can be improved with aging and a degree of doneness of at least 71°C.

2021 ◽  
Author(s):  
Chris Kerth ◽  
Michael C Berto ◽  
Rhonda Miller ◽  
Jeffrey W. Savell

Beef flavor attributes were evaluated in USDA TopChoice and Select beef top loin steaks cut 1.3 cm (THIN) or 3.8 cm (THICK) andcooked on a commercial flat top grill at 177˚C (LOW) or 232˚C (HIGH) grillsurface temperature. Gas chromatography/mass spectrophotometry, was used toevaluate volatile aroma compounds.&nbsp; USDASelect steaks had more 2-octene and less trimethyl pyrazine in (P&lt;0.05) THINsteaks than THICK steaks, while Choice was unaffected by steak thickness(P&gt;0.05).&nbsp; Benzene acetaldehyde washigher and 4-hydroxybenzoic acid was higher in Select LOW grill temperaturescompared to Select HIGH grill temperatures, while 5-methyl-2-furancarboxaldehyde was only present in Choice HIGH grill temperatures (P&lt;0.05).Two acid, three alcohol, one aldehyde, one alkane, and one ketone volatilearoma compounds were higher (P&lt;0.05) for LOW compared to HIGH.&nbsp; Conversely, five alcohols, two aldehyde, twoalkane, all four furans, six ketones, four pyrazines, along with 1H-indole, twopyrroles, two pyridines, and one benzene aroma compounds were higher (P&lt;0.05)in HIGH compared to LOW.&nbsp; Additionally,one alcohol, two aldehydes, one ketones, one sulfur-containing, and six othervolatile compounds were lower, while one acid, one alcohol, one aldehyde, twofurans, one ketone, three pyrazine, one sulfur-containing, and one othervolatile compounds were higher in the THIN compared to THICK.&nbsp; Some aroma compounds like 2-butanone,4-methyl-2-pentanone, 1-ethyl-1H-pyrrole, 1-methyl-1H-pyrrole, and2-methyl-pyridine were only present in THICK cooked HIGH (P&lt;0.05). Steakthickness and grill time are important factors to consider in the developmentof positive Maillard reaction products.@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:roman;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:swiss;mso-font-pitch:variable;mso-font-signature:-536859905 -1073732485 9 0 511 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin:0in;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;}div.WordSection1{page:WordSection1;}@font-face{font-family:"MS Mincho";panose-1:2 2 6 9 4 2 5 8 3 4;mso-font-alt:"MS 明朝";mso-font-charset:128;mso-generic-font-family:modern;mso-font-pitch:fixed;mso-font-signature:-536870145 1791491579 134217746 0 131231 0;}@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:roman;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:swiss;mso-font-pitch:variable;mso-font-signature:-536859905 -1073732485 9 0 511 0;}@font-face{font-family:Cambria;panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:roman;mso-font-pitch:variable;mso-font-signature:-536870145 1073743103 0 0 415 0;}@font-face{font-family:"\@MS Mincho";panose-1:2 2 6 9 4 2 5 8 3 4;mso-font-charset:128;mso-generic-font-family:modern;mso-font-pitch:fixed;mso-font-signature:-536870145 1791491579 134217746 0 131231 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin:0in;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Cambria",serif;mso-ascii-font-family:Cambria;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:"MS Mincho";mso-fareast-theme-font:minor-fareast;mso-hansi-font-family:Cambria;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;}p.Major, li.Major, div.Major{mso-style-name:Major;mso-style-unhide:no;mso-style-qformat:yes;margin:0in;margin-bottom:.0001pt;text-align:center;line-height:200%;mso-pagination:widow-orphan;font-size:14.0pt;mso-bidi-font-size:12.0pt;font-family:"Times New Roman",serif;mso-fareast-font-family:"MS Mincho";mso-fareast-theme-font:minor-fareast;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-family:"Cambria",serif;mso-ascii-font-family:Cambria;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:"MS Mincho";mso-fareast-theme-font:minor-fareast;mso-hansi-font-family:Cambria;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;}div.WordSection1{page:WordSection1;}


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xiaojie Liu ◽  
Nini Hao ◽  
Ruifang Feng ◽  
Zhipeng Meng ◽  
Yanan Li ◽  
...  

Abstract Background Aroma is one the most crucial inherent quality attributes of fruit. ‘Ruixue’ apples were selected from a cross between ‘Pink Lady’ and ‘Fuji’, a later ripening yellow new cultivar. However, there is little known about the content and composition of aroma compounds in ‘Ruixue’ apples or the genetic characters of ‘Ruixue’ and its parents. In addition, the metabolic pathways for biosynthesis of aroma volatiles and aroma-related genes remain poorly understood. Results Volatile aroma compounds were putatively identified using gas chromatography-mass spectrometry (GC–MS). Our results show that the profile of volatile compounds changes with ripening. Aldehydes were the dominant volatile compounds in early fruit development, with alcohols and esters increasing dramatically during maturation. On the basis of a heatmap dendrogram, these aroma compounds clustered into seven groups. In ripe fruit, esters and terpenoids were the main aroma volatiles in ripening fruit of ‘Pink Lady’ and ‘Fuji’ apples, and they included butyl 2-methylbutanoate; propanoic acid, hexyl ester; propanoic acid, hexyl ester; hexanoic acid, hexyl ester; acetic acid, hexyl ester and (Z, E)-α-farnesene. Interestingly, aldehydes and terpenoids were the dominant volatile aroma compounds in ripening fruit of ‘Ruixue’, and they mainly included hexanal; 2-hexenal; octanal; (E)-2-octenal; nonanal and (Z, E)-α-farnesene. By comparing the transcriptome profiles of ‘Ruixue’ and its parents fruits during development, we identified a large number of aroma-related genes related to the fatty acid, isoleucine and sesquiterpenoid metabolism pathways and transcription factors that may volatile regulate biosynthesis. Conclusions Our initial study facilitates a better understanding of the volatile compounds that affect fruit flavour as well as the mechanisms underlying differences in flavour between ‘Ruixue’ and its parents.


2015 ◽  
Vol 69 (6) ◽  
Author(s):  
Eva Vítová ◽  
Kateřina Sůkalová ◽  
Martina Mahdalová ◽  
Lenka Butorová ◽  
Marcela Melikantová

AbstractThirteen cultivars of sea buckthorn (Hippophae rhamnoides L.) berries: Aromat, Botanicky, Buchlovicky, Hergo, Krasavica, Leicora, Ljubitelna, Pavlovsky, Peterbursky, Sluničko, Trofinovsky, Vitaminnaja and Velkoosecky, were tested for the content of volatile aroma compounds using gas chromatography with the solid phase microextraction method during two consequent years (2012- 2013). In total, 69 volatile compounds were identified: 26 alcohols, 12 aldehydes, 11 ketones, 9 acids and 11 esters. Based on principal component analysis, 18 most relevant compounds, best representing the variability of the whole system and suitable for the discrimination of the samples, were selected from all compounds identified. These compounds were then compared using the analysis of variance to confirm differences between the samples. Significant (p < 0.05) differences were found in the varieties in both years, Krasavica and Sluničko cultivars were found to be quite different from other varieties, being rich in the compounds identified and containing most of the selected compounds. Variability within the cultivars (between picking years) was low or not significant.


2020 ◽  
Author(s):  
Xiaojie Liu ◽  
Nini Hao ◽  
Ruifang Feng ◽  
Zhipeng Meng ◽  
Yana Li ◽  
...  

Abstract Background: Aroma is one the most crucial inherent quality attributes of fruit. ‘Ruixue’ apples were selected from a cross between ‘Pink Lady’ and ‘Fuji’, a later ripening yellow new cultivar. However, there is little known about the content and composition of aroma compounds in ‘Ruixue’ apples or the genetic characters of ‘Ruixue’ and its parents. In addition, the metabolic pathways for biosynthesis of aroma volatiles and aroma-related genes remain poorly understood.Results: Volatile aroma compounds were identified using gas chromatography-mass spectrometry (GC-MS). Our results show that the aroma profile of volatile compounds changes with ripening. Aldehydes were the dominant volatile compounds in early fruit development, with alcohols and esters increasing dramatically during maturation. In ripe fruit, esters and terpenoids were the main aroma volatiles in ripening fruit of ‘Pink Lady’ and ‘Fuji’ apples, and they included butyl 2-methylbutanoate, propanoic acid, hexyl ester, propanoic acid, hexyl ester, hexanoic acid, hexyl ester, acetic acid, hexyl ester and (Z, E)-α-farnesene. Interestingly, aldehydes and terpenoids were the dominant volatile aroma compounds in ripening fruit of ‘Ruixue’, and they mainly included 2-hexenal, 2-hexenal, octanal, (E)-2-octenal, nonanal and (Z, E)-α-farnesene. By comparing the transcriptome profiles of ‘Ruixue’ and its parents fruits during development, we identified a large number of aroma-related genes related to the fatty acid, isoleucine and sesquiterpenoid metabolism pathways and transcription factors that may regulate aroma biosynthesis. Conclusions: Our initial study facilitates a better understanding of the volatile aroma compounds that affect fruit flavour as well as the mechanisms underlying differences in flavour between ‘Ruixue’ and its parents.


2011 ◽  
Vol 24 (No. 6) ◽  
pp. 268-274 ◽  
Author(s):  
E. Vítová ◽  
B. Loupancová ◽  
J. Zemanová ◽  
H. Štoudkova ◽  
P. Březina ◽  
...  

Solid-phase microextraction coupled with gas chromatography was used for the analysis of volatile aroma compounds in Niva cheese. The extraction conditions were very mild, which minimises thermal, mechanical, or chemical modification of the sample; the method is rapid, simple, and cheap. In total, 54 compounds were identified in Niva cheese using this method: 3 hydrocarbons, 5&nbsp;aldehydes, 11 ketones, 18 alcohols, 3 esters, 10 fatty acids, and 4 sulphur compounds. These aroma compounds were quantified and subsequently the changes in the concentrations of them were studied throughout the ripening period. Most of the volatile compounds identified were present at all stages of the cheese ripening, their amounts changing significantly, however, in most cases the final concentration in the ripe cheeses was similar to the initial concentration in the unripe cheese. &nbsp;


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