Prednisolone-induced changes in gene-expression profiles in healthy volunteers

2011 ◽  
Vol 12 (7) ◽  
pp. 985-998 ◽  
Author(s):  
Erik JM Toonen ◽  
Wilco WM Fleuren ◽  
Ulla Nässander ◽  
Marie-José C van Lierop ◽  
Susanne Bauerschmidt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Healthy Volunteers ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Induced Changes

scholarly journals Age and Vitamin E-Induced Changes in Gene Expression Profiles of T Cells

2006 ◽  
Vol 177 (9) ◽  
pp. 6052-6061 ◽  
Author(s):  
Sung Nim Han ◽  
Oskar Adolfsson ◽  
Cheol-Koo Lee ◽  
Tomas A. Prolla ◽  
Jose Ordovas ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Vitamin E ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Induced Changes

454 pyrosequencing-based analysis of gene expression profiles in the amphipod Melita plumulosa: Transcriptome assembly and toxicant induced changes

2014 ◽  
Vol 153 ◽  
pp. 73-88 ◽  
Author(s):  
Sharon E. Hook ◽  
Natalie A. Twine ◽  
Stuart L. Simpson ◽  
David A. Spadaro ◽  
Philippe Moncuquet ◽  
...  
Keyword(s):  
Gene Expression ◽  
454 Pyrosequencing ◽  
Transcriptome Assembly ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Induced Changes

scholarly journals Gene expression profiling of the effects of organic dust in lung epithelial and THP-1 cells reveals inductive effects on inflammatory and immune response genes

2016 ◽  
Vol 48 (4) ◽  
pp. 281-289 ◽  
Author(s):  
Vijay Boggaram ◽  
David S. Loose ◽  
Koteswara R. Gottipati ◽  
Kartiga Natarajan ◽  
Courtney T. Mitchell
Keyword(s):  
Gene Expression ◽  
Inflammatory Responses ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Organic Dust ◽  
Monocytic Cells ◽  
Lung Epithelial ◽  
Poultry Dust ◽  
Induced Changes ◽  
Dust Extract

The intensification and concentration of animal production operations expose workers to high levels of organic dusts in the work environment. Exposure to organic dusts is a risk factor for the development of acute and chronic respiratory symptoms and diseases. Lung epithelium plays important roles in the control of immune and inflammatory responses to environmental agents to maintain lung health. To better understand the effects of organic dust on lung inflammatory responses, we characterized the gene expression profiles of A549 alveolar and Beas2B bronchial epithelial and THP-1 monocytic cells influenced by exposure to poultry dust extract by DNA microarray analysis using Illumina Human HT-12 v4 Expression BeadChip. We found that A549 alveolar and Beas2B bronchial epithelial and THP-1 cells responded with unique changes in the gene expression profiles with regulation of genes encoding inflammatory cytokines, chemokines, and other inflammatory proteins being common to all the three cells. Significantly induced genes included IL-8, IL-6, IL-1β, ICAM-1, CCL2, CCL5, TLR4, and PTGS2. Validation by real-time qRT-PCR, ELISA, Western immunoblotting, and immunohistochemical staining of lung sections from mice exposed to dust extract validated DNA microarray results. Pathway analysis indicated that dust extract induced changes in gene expression influenced functions related to cellular growth and proliferation, cell death and survival, and cellular development. These data show that a broad range of inflammatory mediators produced in response to poultry dust exposure can modulate lung immune and inflammatory responses. This is the first report on organic dust induced changes in expression profiles in lung epithelial and THP-1 monocytic cells.

scholarly journals Variability in statin-induced changes in gene expression profiles of pancreatic cancer

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Helena Gbelcová ◽  
Silvie Rimpelová ◽  
Tomáš Ruml ◽  
Marie Fenclová ◽  
Vítek Kosek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pancreatic Cancer ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Induced Changes

scholarly journals Single-Cell Transcript Profiling of Barley Attacked by the Powdery Mildew Fungus

2007 ◽  
Vol 20 (3) ◽  
pp. 235-246 ◽  
Author(s):  
Torben Gjetting ◽  
Peter H. Hagedorn ◽  
Patrick Schweizer ◽  
Hans Thordal-Christensen ◽  
Timothy L. W. Carver ◽  
...  
Keyword(s):  
Gene Expression ◽  
Powdery Mildew ◽  
Expression Profiles ◽  
Cdna Array ◽  
Gene Expression Profiles ◽  
Powdery Mildew Fungus ◽  
Nonspecific Resistance ◽  
Susceptible Host ◽  
Leaf Epidermal Cell ◽  
Induced Changes

In many plant-pathogen interactions, there are several possible outcomes for simultaneous attacks on the same leaf. For instance, an attack by the powdery mildew fungus on one barley leaf epidermal cell may succeed in infection and formation of a functional haustorium, whereas a neighboring cell attacked at the same time may resist fungal penetration. To date, the mixed cellular responses seen even in susceptible host leaves have made it difficult to relate induced changes in gene expression to resistance or susceptibility in bulk leaf samples. By microextraction of cell-specific mRNA and subsequent cDNA array analysis, we have successfully obtained separate gene expression profiles for specific mildew-resistant and -infected barley cells. Thus, for the first time, it is possible to identify genes that are specifically regulated in infected cells and, presumably, involved in fungal establishment. Further, although much is understood about the genetic basis of effective papilla resistance associated with mutant mlo barley, we provide here the first evidence for gene regulation associated with effective papilla-based nonspecific resistance expressed in nominally “susceptible” wild-type barley.

Radiation-Induced Changes in Gene-Expression Profiles for the SCC VII Tumor Cells Grown In Vitro and In Vivo

2006 ◽  
Vol 8 (7-8) ◽  
pp. 1263-1272 ◽  
Author(s):  
John A. Cook ◽  
Eric Y. Chuang ◽  
Mong-Hsun Tsai ◽  
Debbie Coffin ◽  
William Degraff ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tumor Cells ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Radiation Induced ◽  
Induced Changes

Reproducible chemical-induced changes in gene expression profiles in human hepatoma HepaRG cells under various experimental conditions

2009 ◽  
Vol 23 (3) ◽  
pp. 466-475 ◽  
Author(s):  
Carine B. Lambert ◽  
Catherine Spire ◽  
Marie-Pierre Renaud ◽  
Nancy Claude ◽  
Andre Guillouzo
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Human Hepatoma ◽  
Experimental Conditions ◽  
Heparg Cells ◽  
Induced Changes

Abnormal adipokine levels and leptin-induced changes in gene expression profiles in multiple myeloma

2009 ◽  
Vol 83 (5) ◽  
pp. 460-470 ◽  
Author(s):  
Janne E. Reseland ◽  
Sjur Reppe ◽  
Ole K. Olstad ◽  
Henrik Hjorth-Hansen ◽  
Anne T. Brenne ◽  
...  
Keyword(s):  
Gene Expression ◽  
Multiple Myeloma ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Induced Changes

Microarray analysis of gene expression in blood neutrophils of parturient cows

2004 ◽  
Vol 16 (2) ◽  
pp. 212-221 ◽  
Author(s):  
Sally A. Madsen ◽  
Ling-Chu Chang ◽  
Mary-Clare Hickey ◽  
Guilherme J. M. Rosa ◽  
Paul M. Coussens ◽  
...  
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
Mixed Model ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Rt Pcr ◽  
Altered Expression ◽  
Mixed Model Approach ◽  
Blood Neutrophils ◽  
Induced Changes

It is well documented that blood neutrophils from parturient dairy cows do not perform as well as neutrophils from nonparturient cows in laboratory assays of adhesion, migration, or phagocytosis-induced respiratory burst. However, little is known about the possible molecular basis for parturition-induced changes in neutrophils. cDNA microarray analysis was used in the current study to explore parturition-induced changes in gene expression profiles in bovine blood neutrophils. Total RNA from isolated blood neutrophils of four parturient Holstein cows was obtained before, during, and after parturition, reverse transcribed into cDNA, and sequentially labeled with Cy3 or Cy5 dyes prior to paired hybridizations to 1,056 member bovine total leukocyte (BOTL-3) microarrays in a loop design. Resulting gene expression data were LOWESS normalized by array and analyzed using a mixed model approach. Results showed that expression profiles for 302 BOTL-3 genes were influenced by parturition. BLASTn analysis and preliminary clustering of affected genes by biological function indicated that the largest proportion (14%) of changed genes encode proteins critical to regulation of apoptosis. Independent confirmation of altered expression for 16 of these genes was achieved using quantitative real-time RT-PCR (Q-RT-PCR). A predominantly survival phenotype inferred from the microarray and Q-RT-PCR results was substantiated by monitoring apoptosis status of blood neutrophils from castrated male cattle cultured in the presence of sera from parturient cows. Thus our combined gene expression and apoptosis phenotyping results suggest that bovine parturition may induce prolonged survival in normally short-lived blood neutrophils.

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