Specific targeting and noninvasive imaging of breast cancer stem cells using single-walled carbon nanotubes as novel multimodality nanoprobes

Nanomedicine ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. 31-46 ◽  
Author(s):  
Achraf Al Faraj ◽  
Asma Sultana Shaik ◽  
Baraa Al Sayed ◽  
Rabih Halwani ◽  
Ibrahim Al Jammaz
Keyword(s):  
Breast Cancer ◽  
Carbon Nanotubes ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Noninvasive Imaging ◽  
Single Walled Carbon Nanotubes ◽  
Breast Cancer Stem Cells ◽  
Single Walled Carbon ◽  
Specific Targeting ◽  
Walled Carbon Nanotubes

scholarly journals Dragging Human Mesenchymal Stem Cells with the Aid of Supramolecular Assemblies of Single-Walled Carbon Nanotubes, Molecular Magnets, and Peptides in a Magnetic Field

2015 ◽  
Vol 2015 ◽  
pp. 1-9
Author(s):  
Ana Cláudia C. de Paula ◽  
Gustavo A. M. Sáfar ◽  
Alfredo M. Góes ◽  
Marcelo P. Bemquerer ◽  
Marcos A. Ribeiro ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Carbon Nanotubes ◽  
Stem Cells ◽  
Supramolecular Assembly ◽  
Single Walled Carbon Nanotubes ◽  
Molecular Magnets ◽  
Multiwalled Carbon ◽  
Blood Capillaries ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection. So a challenge in stem cells therapy is the delivery of cells to the organ of interest, a specific site. The aim of this paper was to investigate the effects of a supramolecular assembly composed of single-walled carbon nanotubes (SWCNT), molecular magnets (lawsone-Co-phenanthroline), and a synthetic peptide (FWYANHYWFHNAFWYANHYWFHNA) in the hASCs cultures. The hASCs were isolated, characterized, expanded, and cultured with the SWCNT supramolecular assembly (SWCNT-MA). The assembly developed did not impair the cell characteristics, viability, or proliferation. During growth, the cells were strongly attached to the assembly and they could be dragged by an applied magnetic field of less than 0.3 T. These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body. This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

Photoablation of Glioblastoma Stem Cells by Single-Walled Carbon Nanotubes Functionalized with CD133 Antibody

2017 ◽  
pp. 389-410
Author(s):  
Chung-Hao Wang ◽  
Shih-Hwa Chiou ◽  
Chen-Pin Chou ◽  
Yu-Chih Chen ◽  
Yao-Jhang Huang ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Stem Cells ◽  
Single Walled Carbon Nanotubes ◽  
Glioblastoma Stem Cells ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

scholarly journals Targeting single-walled carbon nanotubes for the treatment of breast cancer using photothermal therapy

2013 ◽  
Vol 24 (37) ◽  
pp. 375104 ◽  
Author(s):  
Luís F F Neves ◽  
John J Krais ◽  
Brent D Van Rite ◽  
Rajagopal Ramesh ◽  
Daniel E Resasco ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Carbon Nanotubes ◽  
Photothermal Therapy ◽  
Single Walled Carbon Nanotubes ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

scholarly journals Targeted Single-Walled Carbon Nanotubes for Photothermal Therapy Combined with Immune Checkpoint Inhibition for the Treatment of Metastatic Breast Cancer

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Patrick McKernan ◽  
Needa A. Virani ◽  
Gabriela N. F. Faria ◽  
Clément G. Karch ◽  
Ricardo Prada Silvy ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Carbon Nanotubes ◽  
Metastatic Breast Cancer ◽  
Photothermal Therapy ◽  
Metastatic Breast ◽  
Single Walled Carbon Nanotubes ◽  
Maximum Temperature ◽  
Single Walled Carbon ◽  
Photothermal Ablation ◽  
Walled Carbon Nanotubes

AbstractThe greatest contributors to cancer mortality are metastasis and the consequences of its treatment. Here, we present a novel treatment of metastatic breast cancer that combines photothermal therapy with targeted single-walled carbon nanotubes (SWCNTs) and immunostimulation with a checkpoint inhibitor. We find that the selective near-infrared photothermal ablation of primary orthotopic EMT6 breast tumors in syngeneic BALB/cJ mice using an annexin A5 (ANXA5) functionalized SWCNT bioconjugate synergistically enhances an anti-cytotoxic T-lymphocyte-associated protein 4 (anti-CTLA-4)-dependent abscopal response, resulting in an increased survival (55%) at 100 days after tumor inoculation. In comparison, there was no survival at 100 days for either photothermal therapy by itself or immunostimulation by itself. Prior to photothermal therapy, the SWCNT-ANXA5 bioconjugate was administered systemically at a relatively low dose of 1.2 mg/kg, where it then accumulated in tumor vasculature via ANXA5-dependent binding. During photothermal therapy, the average maximum temperature in the tumor reached 54 °C (duration 175 s). The mechanism of prolonged survival resulting from combinatorial photothermal ablation and immune stimulation was evaluated by flow cytometric quantification of splenic antitumoral immune effector cells and serum cytokine quantification.

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