Development and in vivo imaging of a PET/MRI nanoprobe with enhanced NIR fluorescence by dye encapsulation

Nanomedicine ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. 219-229 ◽  
Author(s):  
Jun Sung Kim ◽  
Young-Hwa Kim ◽  
Jin Hyun Kim ◽  
Keon Wook Kang ◽  
Eunju Lee Tae ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Nir Fluorescence

Activatable NIR Fluorescence/MRI Bimodal Probes for in Vivo Imaging by Enzyme-Mediated Fluorogenic Reaction and Self-Assembly

2019 ◽  
Vol 141 (26) ◽  
pp. 10331-10341 ◽  
Author(s):  
Runqi Yan ◽  
Yuxuan Hu ◽  
Fei Liu ◽  
Shixuan Wei ◽  
Daqing Fang ◽  
...  
Keyword(s):  
Self Assembly ◽  
In Vivo Imaging ◽  
Nir Fluorescence ◽  
Fluorogenic Reaction

scholarly journals Rational design of a NIR-II fluorescent nano-system with maximized fluorescence performance and applications

2021 ◽  
Author(s):  
Haoli Yu ◽  
Yuesong Wang ◽  
Yan Chen ◽  
Min Ji
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Rational Design ◽  
Near Infrared ◽  
Nir Fluorescence

Purpose: Near-infrared (NIR) fluorescence imaging (FI) become a research hotspot in the field of in vivo imaging. Here, we intend to synthesize a NIR-II fluorescence nano-system with an excellent fluorescence...

Near-infrared Emitting Gold-silver Nanoclusters with Large Stokes Shift for Two-Photon in Vivo Imaging

2021 ◽  
Author(s):  
Yaowei Peng ◽  
Xiaoyu Huang ◽  
Fu Wang
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Stokes Shift ◽  
Silver Nanoclusters ◽  
One Pot ◽  
Two Photon ◽  
Nir Fluorescence ◽  
Gold Silver ◽  
Stokes Shifts

Near-infrared emitting bi-metallic gold/silver nanoclusters with great stokes shifts were manufactured through one-pot synthesis. The gold/silver nanoclusters exhibit strong NIR fluorescence due to the silver effect, which can be applied...

A fluorinated aza-BODIPY derivative for NIR fluorescence/PA/19F MR tri-modality in vivo imaging

2019 ◽  
Vol 55 (42) ◽  
pp. 5851-5854 ◽  
Author(s):  
Lianhua Liu ◽  
Yaping Yuan ◽  
Yuqi Yang ◽  
Michael T. McMahon ◽  
Shizhen Chen ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Small Molecule ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Tumor Imaging ◽  
Near Infrared Fluorescence ◽  
Highly Efficient ◽  
Nir Fluorescence

A fluorinated aza-BODIPY derivative BDPF was developed as a small molecule contrast agent, which displayed highly efficient near infrared fluorescence/photoacoustic/19F MR tri-modality tumor imaging.

scholarly journals In Vivo Imaging of Click-Crosslinked Hydrogel Depots Following Intratympanic Injection

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3070
Author(s):  
Hyeon Jin Ju ◽  
Mina Park ◽  
Ji Hoon Park ◽  
Gi Ru Shin ◽  
Hak Soo Choi ◽  
...  
Keyword(s):  
Hearing Loss ◽  
Hyaluronic Acid ◽  
Residence Time ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Tympanic Cavity ◽  
Nir Fluorescence ◽  
Degradation Pattern ◽  
Intratympanic Injection

In this study, we developed injectable intratympanic hyaluronic acid (HA) depots for the treatment of hearing loss. We prepared an injectable click-crosslinking formulation by modifying HA with tetrazine (HA-TET) and trans-cyclooctene (HA-TCO), which crosslinked to form an HA depot (Cx-HA). Preparation of the click-crosslinking HA formulation was facile, and Cx-HA depot formation was reproducible. Additionally, the Cx-HA hydrogel was significantly stiffer than HA hydrogel. To monitor the degradation pattern of hydrogels, we mixed a zwitterionic near-infrared (NIR) fluorophore (e.g., ZW800-1C) in the click-crosslinking HA formulation. Then, HA-TET and HA-TCO solutions containing ZW800-1C were loaded separately into the compartments of a dual-barrel syringe for intratympanic injection. The Cx-HA depots formed quickly, and an extended residence time in the tympanic cavity was confirmed by performing NIR fluorescence imaging. We have successfully prepared an injectable click-crosslinking HA formulation that has promise as an intratympanic drug depot.

scholarly journals Er3+-Ions-Doped Multiscale Nanoprobes for Fluorescence Imaging in Cellular and Living Mice

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2676
Author(s):  
Cong Cao ◽  
Yu Xie ◽  
Shi-Wen Li ◽  
Chang Hong
Keyword(s):  
Light Scattering ◽  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Diagnostic Techniques ◽  
Single Nanoparticle ◽  
Nir Fluorescence ◽  
Nir Luminescence

With the development of biotechnology, luminescent nanoprobes for biological disease detection are widely used. However, the further application in clinic is limited by the reduced penetration depth in the tissues and light scattering. In this work, we have synthesized NaYF4:Yb,Er,Ce@SiO2-OAlg nanomaterials, which have both upconversion and near-infrared (NIR) luminescence. The optimized probes were determined to achieve cell imaging by its upconversion (UCL) luminescence and in vivo imaging through collection of NIR fluorescence signals simultaneously. The research is conducive to developing accurate diagnostic techniques based on UCL and NIR fluorescence imaging by a single nanoparticle.

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

In vivo imaging of beta-amyloid load in transgenic rodents with [F-18]FDDNP microPET

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S588-S588
Author(s):  
Vladimir Kepe ◽  
Gregory M Cole ◽  
Jie Liu ◽  
Dorothy G Flood ◽  
Stephen P Trusko ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Beta Amyloid ◽  
Amyloid Load

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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