Targeted removal of migratory tumor cells by functionalized magnetic nanoparticles impedes metastasis and tumor progression

Nanomedicine ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Kenneth E Scarberry ◽  
Roman Mezencev ◽  
John F McDonald
Keyword(s):  
Magnetic Nanoparticles ◽  
Tumor Progression ◽  
Tumor Cells ◽  
Functionalized Magnetic Nanoparticles

Peptide-based isolation of circulating tumor cells by magnetic nanoparticles

2014 ◽  
Vol 2 (26) ◽  
pp. 4080-4088 ◽  
Author(s):  
Linling Bai ◽  
Yimeng Du ◽  
Jiaxi Peng ◽  
Yi Liu ◽  
Yanmei Wang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Efficiency ◽  
Isolation Method ◽  
Functionalized Magnetic Nanoparticles

A new CTC isolation method with high efficiency by using EpCAM recognition peptide functionalized magnetic nanoparticles was developed.

Functionalized magnetic nanoparticles for biomedical applications

2015 ◽  
Vol 21 (42) ◽  
pp. 6038-6054 ◽  
Author(s):  
Dragoș Gudovan ◽  
Paul Balaure ◽  
Dan Mihăiescu ◽  
Adrian Fudulu ◽  
Bogdan Purcăreanu ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Biomedical Applications ◽  
Functionalized Magnetic Nanoparticles

scholarly journals EphA2 super-enhancer promotes tumor progression by recruiting FOSL2 and TCF7L2 to activate the target gene EphA2

2021 ◽  
Vol 12 (3) ◽  
Author(s):  
Shuang Cui ◽  
Qiong Wu ◽  
Ming Liu ◽  
Mu Su ◽  
ShiYou Liu ◽  
...  
Keyword(s):  
Tumor Progression ◽  
Tumor Cells ◽  
Target Gene ◽  
Super Enhancer ◽  
Proliferation And Metastasis ◽  
Active Transcription ◽  
Hct 116 ◽  
Large Clusters

AbstractSuper-enhancers or stretch enhancers (SEs) consist of large clusters of active transcription enhancers which promote the expression of critical genes that define cell identity during development and disease. However, the role of many super-enhancers in tumor cells remains unclear. This study aims to explore the function and mechanism of a new super-enhancer in various tumor cells. A new super-enhancer that exists in a variety of tumors named EphA2-Super-enhancer (EphA2-SE) was found using multiple databases and further identified. CRISPR/Cas9-mediated deletion of EphA2-SE results in the significant downregulation of its target gene EphA2. Mechanistically, we revealed that the core active region of EphA2-SE comprises E1 component enhancer, which recruits TCF7L2 and FOSL2 transcription factors to drive the expression of EphA2, induce cell proliferation and metastasis. Bioinformatics analysis of RNA-seq data and functional experiments in vitro illustrated that EphA2-SE deletion inhibited cell growth and metastasis by blocking PI3K/AKT and Wnt/β-catenin pathway in HeLa, HCT-116 and MCF-7 cells. Overexpression of EphA2 in EphA2-SE−/− clones rescued the effect of EphA2-SE deletion on proliferation and metastasis. Subsequent xenograft animal model revealed that EphA2-SE deletion suppressed tumor proliferation and survival in vivo. Taken together, these findings demonstrate that EphA2-SE plays an oncogenic role and promotes tumor progression in various tumors by recruiting FOSL2 and TCF7L2 to drive the expression of oncogene EphA2.

scholarly journals Iron-Bound Lipocalin-2 from Tumor-Associated Macrophages Drives Breast Cancer Progression Independent of Ferroportin

Metabolites ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 180
Author(s):  
Christina Mertens ◽  
Matthias Schnetz ◽  
Claudia Rehwald ◽  
Stephan Grein ◽  
Eiman Elwakeel ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Progression ◽  
Tumor Cells ◽  
Cancer Progression ◽  
Lung Metastases ◽  
Expression Profiles ◽  
The Cancer Genome Atlas ◽  
Lipocalin 2 ◽  
Tumor Associated Macrophages

Macrophages supply iron to the breast tumor microenvironment by enforced secretion of lipocalin-2 (Lcn-2)-bound iron as well as the increased expression of the iron exporter ferroportin (FPN). We aimed at identifying the contribution of each pathway in supplying iron for the growing tumor, thereby fostering tumor progression. Analyzing the expression profiles of Lcn-2 and FPN using the spontaneous polyoma-middle-T oncogene (PyMT) breast cancer model as well as mining publicly available TCGA (The Cancer Genome Atlas) and GEO Series(GSE) datasets from the Gene Expression Omnibus database (GEO), we found no association between tumor parameters and Lcn-2 or FPN. However, stromal/macrophage-expression of Lcn-2 correlated with tumor onset, lung metastases, and recurrence, whereas FPN did not. While the total iron amount in wildtype and Lcn-2−/− PyMT tumors showed no difference, we observed that tumor-associated macrophages from Lcn-2−/− compared to wildtype tumors stored more iron. In contrast, Lcn-2−/− tumor cells accumulated less iron than their wildtype counterparts, translating into a low migratory and proliferative capacity of Lcn-2−/− tumor cells in a 3D tumor spheroid model in vitro. Our data suggest a pivotal role of Lcn-2 in tumor iron-management, affecting tumor growth. This study underscores the role of iron for tumor progression and the need for a better understanding of iron-targeted therapy approaches.

High-Performance Functionalized Magnetic Nanoparticles with Tailored Sizes and Shapes for Localized Hyperthermia Applications

2021 ◽  
Author(s):  
Izabell Crăciunescu ◽  
Petru Palade ◽  
Nicuşor Iacob ◽  
George Marian Ispas ◽  
Anda Elena Stanciu ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
High Performance ◽  
Functionalized Magnetic Nanoparticles

scholarly journals Stromal CCL5 Promotes Breast Cancer Progression by Interacting with CCR3 in Tumor Cells

2021 ◽  
Vol 22 (4) ◽  
pp. 1918
Author(s):  
Mio Yamaguchi ◽  
Kiyoshi Takagi ◽  
Koki Narita ◽  
Yasuhiro Miki ◽  
Yoshiaki Onodera ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Carcinoma ◽  
Tumor Progression ◽  
Tumor Cells ◽  
Cancer Progression ◽  
Stromal Cells ◽  
Human Breast Carcinoma ◽  
Breast Cancer Patients ◽  
Breast Carcinomas ◽  
Human Breast

Chemokines secreted from stromal cells have important roles for interactions with carcinoma cells and regulating tumor progression. C-C motif chemokine ligand (CCL) 5 is expressed in various types of stromal cells and associated with tumor progression, interacting with C-C chemokine receptor (CCR) 1, 3 and 5 expressed in tumor cells. However, the expression on CCL5 and its receptors have so far not been well-examined in human breast carcinoma tissues. We therefore immunolocalized CCL5, as well as CCR1, 3 and 5, in 111 human breast carcinoma tissues and correlated them with clinicopathological characteristics. Stromal CCL5 immunoreactivity was significantly correlated with the aggressive phenotype of breast carcinomas. Importantly, this tendency was observed especially in the CCR3-positive group. Furthermore, the risk of recurrence was significantly higher in the patients with breast carcinomas positive for CCL5 and CCR3 but negative for CCR1 and CCR5, as compared with other patients. In summary, the CCL5-CCR3 axis might contribute to a worse prognosis in breast cancer patients, and these findings will contribute to a better understanding of the significance of the CCL5/CCRs axis in breast carcinoma microenvironment.

scholarly journals Cellulase immobilization on TEOS encapsulated and APTES functionalized magnetic nanoparticles (MNPs)

2021 ◽  
Vol 1092 (1) ◽  
pp. 012071
Author(s):  
Shah Samiur Rashid ◽  
Md. Belal Hossain Sikder ◽  
Mohd Hasbi Bin Ab. Rahim ◽  
Aizi Nor Mazila Binti Ramli ◽  
Rashidi Bin Roslan
Keyword(s):  
Magnetic Nanoparticles ◽  
Functionalized Magnetic Nanoparticles
Sign in / Sign up

Export Citation Format

Share Document