scholarly journals Adipose-derived stem cells promote lymphangiogenesis in response to VEGF-C stimulation or TGF-β1 inhibition

2011 ◽  
Vol 7 (12) ◽  
pp. 1457-1473 ◽  
Author(s):  
Alan Yan ◽  
Tomer Avraham ◽  
Jamie C Zampell ◽  
Yosef S Haviv ◽  
Evan Weitman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Tgf Β1

scholarly journals The effect of inhibiting exosomes derived from adipose-derived stem cells via the TGF-β1/Smad pathway on the fibrosis of keloid fibroblasts

Gland Surgery ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 1046-1056
Author(s):  
Zhi-Yuan Wu ◽  
Hui-Jun Zhang ◽  
Zhi-Hong Zhou ◽  
Zhan-Peng Li ◽  
Si-Mu Liao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Smad Pathway ◽  
Tgf Β1 ◽  
Keloid Fibroblasts

scholarly journals Differential effects of BMP-2 and TGF-β1 on chondrogenic differentiation of adipose derived stem cells

2007 ◽  
Vol 40 (6) ◽  
pp. 809-823 ◽  
Author(s):  
A. T. Mehlhorn ◽  
P. Niemeyer ◽  
K. Kaschte ◽  
L. Muller ◽  
G. Finkenzeller ◽  
...  
Keyword(s):  
Stem Cells ◽  
Chondrogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Differential Effects ◽  
Tgf Β1

scholarly journals TGF-β1 Regulates Smooth Muscle Cells Differentiation from Adipose-Derived Stem Cells via the Wnt/β-Catenin Pathway

2021 ◽  
Author(s):  
Xuling Lv ◽  
Hao Chen ◽  
Zikai Zhang ◽  
Tian Li ◽  
Qing Wei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Pelvic Floor ◽  
Signaling Pathway ◽  
Myogenic Differentiation ◽  
Pelvic Floor Dysfunction ◽  
Muscle Cells ◽  
Adipose Derived Stem Cells ◽  
Tgf Β1

Abstract Background: Pelvic floor dysfunction (PFD) is a spectrum of disorders including stress urinary incontinence and pelvic organ prolapse. Transforming growth factor-β1 (TGF-β1) can induce mesenchymal stem cells (MSCs) to differentiate into smooth muscle cells (SMCs). SMCs derived from adipose-derived stem cells (ADSCs) can be used to repair damaged pelvic floor smooth muscle tissues, which is of great interest for clinical applications using stem cell therapy for PFD. The Wnt/β-catenin pathway acts as a decisive factor in the fate of stem cells.Methods and Results: In this study, we used medium containing TGF-β1, TGF-β1 inhibitor LY2109761, or Wnt/β-catenin inhibitor KYA1797K, to induce ADCSs to differentiate into SMCs in vitro to explore the influence of TGF-β1 on the myogenic differentiation of ADCSs via the Wnt/β-catenin pathway. Results: 1) TGF-β1 induces ADSC-derived SMCs to hyper-express the SMC markers including SMA-α, Desmin, Calponin, and SMMHC ; 2) TGF-β1 activates the Wnt/β-catenin signaling pathway in ADSCs. After blocking TGF-β1, the Wnt/β-catenin pathway and myogenic differentiation in cells were inhibited; 3) the Wnt/β-catenin pathway is involved in the differentiation of ADSCs into SMCs. After differentiation induction, the synchronized changes in the activation of Wnt/β-catenin signaling and the expression of SMC-specific proteins showed a trend of simultaneous changes, and after the inhibition of the Wnt pathway, the adult muscle differentiation was significantly inhibited.Conclusions: We established a simpler and more efficient method for inducing ADSCs to differentiate into SMCs using TGF-β1 and demonstrated that the Wnt/β-catenin signaling pathway is activated during this process.

The suppression effect of dendritic cells maturation by adipose-derived stem cells through TGF-β1 related pathway

2018 ◽  
Vol 370 (2) ◽  
pp. 708-717 ◽  
Author(s):  
Yu-Chi Wang ◽  
Rong-Fu Chen ◽  
Gerald Brandacher ◽  
W.P. Andrew Lee ◽  
Yur-Ren Kuo
Keyword(s):  
Stem Cells ◽  
Dendritic Cells ◽  
Adipose Derived Stem Cells ◽  
Suppression Effect ◽  
Related Pathway ◽  
Tgf Β1

Combined delivery of FGF-2, TGF-β1, and adipose-derived stem cells from an engineered periosteum to a critical-sized mouse femur defect

2016 ◽  
Vol 105 (3) ◽  
pp. 900-911 ◽  
Author(s):  
Raimundo Romero ◽  
John K. Travers ◽  
Emilie Asbury ◽  
Attie Pennybaker ◽  
Laura Chubb ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Tgf Β1

scholarly journals Adipose-Derived Stem Cells Alleviate Radiation-Induced Muscular Fibrosis by Suppressing the Expression of TGF-β1

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Wei Sun ◽  
Xinchu Ni ◽  
Suping Sun ◽  
Leiming Cai ◽  
Jingping Yu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Transforming Growth Factor ◽  
Adipose Derived Stem Cells ◽  
Peripheral Tissues ◽  
Muscle Fibrosis ◽  
Muscle Tissues ◽  
Radiation Induced ◽  
Skeletal Muscle Fibrosis ◽  
Tgf Β1

We aim to investigate the effects of adipose-derived stem cells (ASCs) transplantation on irradiation-induced skeletal muscle fibrosis. Sixty-four rabbits were randomly divided into ASCs group and PBS group followed by irradiation at unilateral hip with a single dose of 80 Gy. Nonirradiated side with normal skeletal muscle served as normal control. Skeletal muscle tissues were collected from eight rabbits in each group at 1 w, 4 w, 8 w, and 26 w after irradiation. Migration of ASCs was observed in the peripheral tissues along the needle passage in the injured muscle. The proportion of the area of collagen fibers to the total area in sections of ASCs group was lower than those of PBS groups at 4 w, 8 w, and 26 w after irradiation. Significant decrease was noted in the integrated optimal density of the transforming growth factorβ1 (TGF-β1) in the ASCs group compared with those of PBS group at 4 w, 8 w, and 26 w after irradiation. Moreover, the expression of TGF-β1 was lower in the ASCs group compared to those of the PBS group at each time point determined by Western blot analysis. ASCs transplantation could alleviate irradiation fibrosis by suppressing the level of TGF-β1 in the irradiated skeletal muscle.

scholarly journals Adipose-Derived Stem Cells Promote Bone Coupling in Bisphosphonate-Related Osteonecrosis of the Jaw by TGF-β1

2021 ◽  
Vol 9 ◽  
Author(s):  
Xian Dong ◽  
Linhai He ◽  
Xiaolong Zang ◽  
Yang He ◽  
Jingang An ◽  
...  
Keyword(s):  
Stem Cells ◽  
Animal Model ◽  
Conditioned Medium ◽  
Bone Matrix ◽  
Neutralizing Antibody ◽  
Clinical Samples ◽  
Therapeutic Effects ◽  
Adipose Derived Stem Cells ◽  
Tgf Β1

This study aimed to investigate molecularly targeted therapy to revive bone remodeling and prevent BRONJ by local adipose-derived stem cells (ADSCs) transplantation. Clinical samples of BRONJ and healthy jawbones were used to examine the bone coupling-related cells and TGF-β1 expression. Bone coupling-related cells and TGF-β1 expression were also assessed in BRONJ-like animal model to confirm the results in clinical samples. ADSCs were locally administered in vivo and the therapeutic effects were evaluated by gross observation, radiological imaging, and histological examination. Furthermore, ADSCs-conditioned medium (ADSCs-CM) and neutralizing antibody were applied to assess the effects of ADSCs-derived TGF-β1 on restoring bone coupling in vivo. Osteoclast formation and resorption assays were performed to evaluate the effects of ADSCs-derived TGF-β1 on ZA-treated pre-osteoclasts. Cell migration was performed to assess the effects of ADSCs-derived TGF-β1 on patients’ bone marrow stem cells (BMSCs). The number of osteoclasts, Runx2-positive bone-lining cells (BLCs) and TGF-β1 expression were decreased in BRONJ and animal model jaw bone samples. These reductions were significantly rescued and necrotic jawbone healing was effectively promoted by local ADSCs administration in BRONJ-like animal models. Mechanistically, ADSCs-CM mainly contributed to promoting bone coupling, while TGF-β1 neutralizing antibody in the conditioned medium inhibited these effects. Besides, osteoclastogenesis and patients’ BMSCs migration were also rescued by ADSCs-derived TGF-β1. Furthermore, bone resorption-released bone matrix TGF-β1, together with ADSCs-derived TGF-β1, synergistically contributed to rescuing BMSCs migration. Collectively, ADSCs promoted bone healing of BRONJ by TGF-β1-activated osteoclastogenesis and BMSCs migration capacities.

scholarly journals Cartilage Regeneration of Adipose-Derived Stem Cells in the TGF-β1-Immobilized PLGA-Gelatin Scaffold

2014 ◽  
Vol 11 (3) ◽  
pp. 453-459 ◽  
Author(s):  
Feng Yin ◽  
Junfeng Cai ◽  
Wen Zen ◽  
Yanhui Wei ◽  
Wei Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cartilage Regeneration ◽  
Adipose Derived Stem Cells ◽  
Tgf Β1
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