Fecal microbiota analysis: an overview of sample collection methods and sequencing strategies

2015 ◽  
Vol 10 (9) ◽  
pp. 1485-1504 ◽  
Author(s):  
Vincent Thomas ◽  
James Clark ◽  
Joël Doré
Keyword(s):  
Fecal Microbiota ◽  
Sample Collection ◽  
Microbiota Analysis ◽  
Collection Methods

PREDIKSI PERINGKAT OBLIGASI SYARIAH DI INDONESIA

2014 ◽  
Vol 11 (3) ◽  
pp. 1-15
Author(s):  
Ike Arisanti ◽  
Isti Fadah ◽  
Novi Puspitasari
Keyword(s):  
Profit Sharing ◽  
Sample Collection ◽  
Purposive Sampling ◽  
Financial Factors ◽  
Financial Factor ◽  
Independent Variable ◽  
Rating Prediction ◽  
Collection Methods

This study purposes to analyze the influence of financial and non financial factors to prediction of the rating islamic bond in indonesia. The study used independent variable,that is financial factor (growth, size, profit sharing/fee, liquidity) and non financial factor ( secure and maturity) and dependent variable that is the rating of islamic bond. This study applied logistic regresion analysis with sample collection methods using purposive sampling. After selecting fixed criterias, there were 25 islamic bonds chosen with the numbers of 75 investigation from periods of 2010-2012. The result of this study showed that significantly effect the variable growth (X1) , size(X2), profit sharing/ fee (X3), liquidity (X4), secure (X5), maturity (X6) simultaneously to the rating prediction of islamic bond in indonesia. Partially, variable variables of growth (X1) , size (X2), profit sharing/ fee (X3) which referred not significant affecting to the rating prediction of islamic bond in indonesia. Meanwhile, variables of liquidity (X4), secure (X5), maturity ( X6) referred significant affecting to the rating prediction of islamic bond in indonesia.

scholarly journals Thyroglobulin measurement on fine-needle washout fluids: Influence of sample collection methods

2009 ◽  
Vol 37 (1) ◽  
pp. 42-44 ◽  
Author(s):  
Luca Giovanella ◽  
Luca Ceriani ◽  
Sergio Suriano ◽  
Stefano Crippa
Keyword(s):  
Sample Collection ◽  
Fine Needle ◽  
Collection Methods

scholarly journals Effect of different sample collection methods on the results of raw milk analysis

2013 ◽  
Vol 68 (394) ◽  
pp. 40-44 ◽  
Author(s):  
Jakeline Fernandes Cabral ◽  
Marco Antônio Pereira da Silva ◽  
Rafaella Belchior Brasil ◽  
Thiago Soares Carvalho ◽  
Cristiane Isabô Giovannini ◽  
...  
Keyword(s):  
Raw Milk ◽  
Milk Analysis ◽  
Sample Collection ◽  
Collection Methods

scholarly journals The effect of different sample collection methods on rabbit blood parameters

2020 ◽  
Vol 27 (11) ◽  
pp. 3157-3160
Author(s):  
Martin Massányi ◽  
Ladislav Kohút ◽  
María-José Argente ◽  
Marko Halo ◽  
Anton Kováčik ◽  
...  
Keyword(s):  
Blood Parameters ◽  
Sample Collection ◽  
Rabbit Blood ◽  
Collection Methods

scholarly journals Comparison of Methods To Collect Fecal Samples for Microbiome Studies Using Whole-Genome Shotgun Metagenomic Sequencing

mSphere ◽  
2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Doratha A. Byrd ◽  
Rashmi Sinha ◽  
Kristi L. Hoffman ◽  
Jun Chen ◽  
Xing Hua ◽  
...  
Keyword(s):  
Ambient Temperature ◽  
Fecal Sample ◽  
Gold Standard ◽  
Beta Diversity ◽  
Whole Genome ◽  
Metagenomic Sequencing ◽  
Sample Collection ◽  
Fecal Samples ◽  
Shotgun Metagenomic Sequencing ◽  
Collection Methods

ABSTRACT Few previous studies have assessed stability and “gold-standard” concordance of fecal sample collection methods for whole-genome shotgun metagenomic sequencing (WGSS), an increasingly popular method for studying the gut microbiome. We used WGSS data to investigate ambient temperature stability and putative gold-standard concordance of microbial profiles in fecal samples collected and stored using fecal occult blood test (FOBT) cards, fecal immunochemical test (FIT) tubes, 95% ethanol, or RNAlater. Among 15 Mayo Clinic employees, for each collection method, we calculated intraclass correlation coefficients (ICCs) to estimate stability of fecal microbial profiles after storage for 4 days at ambient temperature and concordance with immediately frozen, no-solution samples (i.e., the putative gold standard). ICCs were estimated for multiple metrics, including relative abundances of select phyla, species, KEGG k-genes (representing any coding sequence that had >70% identity and >70% query coverage with respect to a known KEGG ortholog), KEGG modules, and KEGG pathways; species and k-gene alpha diversity; and Bray-Curtis and Jaccard species beta diversity. ICCs for microbial profile stability were excellent (≥90%) for fecal samples collected via most of the collection methods, except those preserved in 95% ethanol. Concordance with the immediately frozen, no-solution samples varied for all collection methods, but the number of observed species and the beta diversity metrics tended to have higher concordance than other metrics. Our findings, taken together with previous studies and feasibility considerations, indicated that FOBT cards, FIT tubes, and RNAlater are acceptable choices for fecal sample collection methods in future WGSS studies. IMPORTANCE A major direction for future microbiome research is implementation of fecal sample collections in large-scale, prospective epidemiologic studies. Studying microbiome-disease associations likely requires microbial data to be pooled from multiple studies. Our findings suggest collection methods that are most optimal to be used standardly across future WGSS microbiome studies.

Can Drainage Using a Negative-Pressure Wound Therapy Device Replace Traditional Sample Collection Methods?

2016 ◽  
Vol 17 (5) ◽  
pp. 577-582 ◽  
Author(s):  
Giovanni Cagnoni ◽  
Sara Giordana Rimoldi ◽  
Cristina Pagani ◽  
Claudio Savi ◽  
Fabrizio Stefani ◽  
...  
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Sample Collection ◽  
Wound Therapy ◽  
Collection Methods

Serial Microbiota Analysis after Fecal Microbiota Transplantation in a Child with Down's Syndrome

2017 ◽  
Vol 14 (03) ◽  
pp. 136-139
Author(s):  
Lisethe Meijer ◽  
Clementien Vermont ◽  
Andries Budding ◽  
Chris Mulder ◽  
Tim Meij ◽  
...  
Keyword(s):  
Fecal Microbiota Transplantation ◽  
Diversity Index ◽  
Down's Syndrome ◽  
Down’S Syndrome ◽  
Fecal Microbiota ◽  
Recurrent Clostridium Difficile Infection ◽  
Microbiota Analysis ◽  
Before And After ◽  
Immunocompromised State ◽  
Over Time

AbstractFecal microbiota transplantation (FMT) is a very effective treatment for recurrent Clostridium difficile infection (CDI) in adults. However, there is a paucity of data on FMT in children and associated microbiome changes in this particular group. We describe a child with Down's syndrome and intracranial malignancy, who received FMT for recurrent CDI. Detailed microbiota analysis before and after FMT, and pre- and post-recurrence, linked to microbial communities in the donor feces showed that the patient developed a unique microbiota profile after FMT which was very stable over time despite CDI recurrence and subsequent fidaxomicin therapy. Bacteroidetes were stably acquired from donor feces, while Firmicutes, Actinobacteria, Fusobacteria, Verrucomicrobia, and Proteobacteria were unique to the patient. The diversity of microbiota of the patient increased from a Shannon diversity index of 2.08 pre-FMT to 3.12 post-FMT. Our findings underscore that patients with Down's syndrome may well tolerate and benefit from FMT even in a severely immunocompromised state.

Impact of blood sample collection methods on blood protein profiling studies

2017 ◽  
Vol 471 ◽  
pp. 128-134 ◽  
Author(s):  
Maria Ilies ◽  
Cristina Adela Iuga ◽  
Felicia Loghin ◽  
Vishnu Mukund Dhople ◽  
Thomas Thiele ◽  
...  
Keyword(s):  
Blood Sample ◽  
Protein Profiling ◽  
Blood Protein ◽  
Sample Collection ◽  
Collection Methods
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