scholarly journals Fusidic acid resistance in Staphylococcus aureus nasal carriage strains in nine European countries

2014 ◽  
Vol 9 (6) ◽  
pp. 737-745 ◽  
Author(s):  
Casper DJ den Heijer ◽  
Evelien ME van Bijnen ◽  
W John Paget ◽  
Ellen E Stobberingh
Keyword(s):  
Staphylococcus Aureus ◽  
Fusidic Acid ◽  
Acid Resistance ◽  
Nasal Carriage ◽  
European Countries

scholarly journals Mutations in fusA Associated with Posttherapy Fusidic Acid Resistance in Clostridium difficile

2007 ◽  
Vol 51 (5) ◽  
pp. 1840-1843 ◽  
Author(s):  
T. Norén ◽  
T. Åkerlund ◽  
M. Wullt ◽  
L. G. Burman ◽  
M. Unemo
Keyword(s):  
Staphylococcus Aureus ◽  
Clostridium Difficile ◽  
Fusidic Acid ◽  
In Silico ◽  
Acid Resistance ◽  
Nonsynonymous Substitutions ◽  
Resistant Mutants

ABSTRACT In silico, we identified fusA (2,067 bp) in Clostridium difficile 630. Sequencing of fusA in posttherapy fusidic acid-resistant C. difficile isolates from 12 patients with C. difficile-associated diarrhea (CDAD) identified fusA mutations, one or two nonsynonymous substitutions, or in one case a deletion of one codon associated with resistance. Five of these mutations have previously been described in fusA of fusidic acid-resistant Staphylococcus aureus, but seven were novel fusA mutations. Fusidic acid monotherapy for CDAD seemed to rapidly select conserved resistant mutants.

Molecular analysis of fusidic acid resistance in Staphylococcus aureus

2003 ◽  
Vol 47 (2) ◽  
pp. 463-469 ◽  
Author(s):  
Silke Besier ◽  
Albrecht Ludwig ◽  
Volker Brade ◽  
Thomas A. Wichelhaus
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Analysis ◽  
Fusidic Acid ◽  
Acid Resistance

Fusidic Acid Resistance in Staphylococcus aureus Strains in an Interval of Ten Years (2001-2011)

2012 ◽  
Vol 32 (6) ◽  
pp. 1668-1672 ◽  
Author(s):  
Şebnem NERGİZ ◽  
Selahattin ATMACA ◽  
Tuncer ÖZEKİNCİ ◽  
Alicem TEKİN
Keyword(s):  
Staphylococcus Aureus ◽  
Fusidic Acid ◽  
Acid Resistance

scholarly journals Compensatory Adaptation to the Loss of Biological Fitness Associated with Acquisition of Fusidic Acid Resistance in Staphylococcus aureus

2005 ◽  
Vol 49 (4) ◽  
pp. 1426-1431 ◽  
Author(s):  
Silke Besier ◽  
Albrecht Ludwig ◽  
Volker Brade ◽  
Thomas A. Wichelhaus
Keyword(s):  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Fusidic Acid ◽  
Wild Type Strain ◽  
Elongation Factor ◽  
Acid Resistance ◽  
Resistant Bacteria ◽  
Wild Type ◽  
Compensatory Adaptation

ABSTRACT Recent studies have shown that individual amino acid exchanges within elongation factor G (EF-G) cause fusidic acid resistance in Staphylococcus aureus. The data from the present study illustrate that the fusidic acid resistance-mediating amino acid substitutions P406L and H457Y are associated with a marked impairment of the biological fitness of S. aureus. In particular, strains producing EF-G derivatives with these mutations showed reduced growth, decreased plasma coagulase activity, and an impaired capability to compete with the isogenic wild-type strain. Second-site mutations within EF-G, such as A67T and S416F, that have been encountered in clinical fusidic acid-resistant isolates containing the amino acid exchanges P406L and H457Y, respectively, were shown not to contribute to resistance. Furthermore, the substitution A67T had no impact on the biological fitness in vitro. The exchange S416F, however, was found to function as a fitness-compensating mutation in S. aureus carrying the substitution H457Y in EF-G. In conclusion, the data presented in this report provide evidence at the molecular level that the deleterious effects of fusidic acid resistance-mediating exchanges within EF-G of S. aureus can be reduced considerably by specific compensating mutations in this target protein. This compensatory adaptation most likely plays a significant role in the stabilization of resistant bacteria within a given population.

Prevalence of Nasal Carriage of Staphylococcus aureus and its Antibiotic Susceptibility among Healthcare Workers (HCWs) in Ukraine

2018 ◽  
Vol 2 (1) ◽  
pp. 30-41 ◽  
Author(s):  
A.G. Salmanov ◽  
O.M. Verner ◽  
N.G. Shelkova ◽  
O.I. Nicolska ◽  
V.M. Blahodatny ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Clavulanic Acid ◽  
Fusidic Acid ◽  
Antibiotic Susceptibility ◽  
Healthcare Workers ◽  
Nasal Carriage ◽  
Hospital Environment ◽  
Significant Difference ◽  
Amoxicillin Clavulanic Acid ◽  
Hospital Acquired

BACKGROUND. Nasal carriage of Staphylococcus aureus among hospital personnel is a common cause of hospital acquired infections. Emergence of drug resistant strains especially methicillin resistant S. aureus (MRSA) is a serious problem in hospital environment. Therefore, the aim of this study was to determine the prevalence of nasal carriage of Staphylococcus aureus its antibiotic susceptibility among healthcare workers (HCWs) in Ukraine. METHODS. This cross-sectional study was conducted from January to December 2017. The study included medical workers from 19 hospitals in different Ukrainian regions. Nasal swabs were taken from 755 randomly selected HCWs. The mean age of participants was 32.41 ± 8.29 years (range 19—74 years) with a male-to- female ratio of 0.47. The isolates were identified as S. aureus based on morphology, Gram stain, catalase test, coagulase test, and mannitol salt agar fermentation. The sensitivity patterns of S. aureus strains were determined by disk diffusion method (Kirby — Bauer). The panel of antibiotics used in sensitivity tests included: penicillin, oxacillin, cefoxitin, amoxicillin/clavulanic acid, gentamicin, tobramicin, ciprofloxacin, levofloxacin, moxifloxacin, mupirocin, nitrofurantoin, vancomycin, teicoplanin, fosfomycin, clindamycin, erythromycin, rifampicin, linezolid, tetracycline, tigecycline, trimethoprim/sulphamethoxazole, and fusidic acid. Interpretative criteria were those suggested by the CLSI (Clinical and Laboratory Standards Institute). MRSA were confirmed by detection of the mecA gene by polymerase chain reaction. RESULTS. Nasal screening identified 31.1 % (235/755) S. aureus carriers. Of the 235 nasal carriers of S. aureus, 4 % (196/755) carried MSSA (methicillin-sensitive S. aureus) and 39/755 (16.6 %) carried MRSA. The frequency of MRSA and MSSA carriage also varied according to the department/ward. The highest prevalence of nasal carriage of MRSA was in the surgical wards. The staff of the general, pediatric, cardiovascular, neuro and orthopedic surgery wards together with the emergency department accounted for 56.4 % of all MRSA carriers. There was no significant difference between the sexes (p = 0.247), age (p = 0.817), and years of healthcare service (p = 0.15) with regard to the nasal carriage of MRSA and MSSA. In univariate analysis we divided the hospital departments into: emergency, internal medicine, pediatrics, ICUs, surgery, and non-medical units and found no significant difference between MSSA and MRSA carriers (p = 0.224). In the multivariate analysis, the occupation «nurse» was independently associated with MRSA carriage (p = 0.012, odds ratio 3.6, 95 % confidence interval 1.3—9.7). All the S. aureus isolates recovered from nasal carriers, were susceptible to linezolid, tigecycline, vancomycin, teicoplanin, and mupirocin. Susceptibility to the other antimicrobials was also on a high level: 98.3 % of strains were found susceptible to trimethoprim/sulphamethoxazole, 96.2 % — to nitrofurantoin, 95.3 % — to fusidic acid, 92.3 % — to fosfomicin, 88.5 % — to amoxicillin/clavulanic acid, 87.2 % — to tobramycin, 86.8 % — to clindamycin. Resistance to oxacyllin came up to 16.6 %. CONCLUSIONS. Nasal carriage of S. aureus appears to play a key role in the epidemiology and pathogenesis of infection. HCWs who are at interface between the hospital and the community may serve as agents of cross contamination of hospital acquired and community acquired MRSA. It is of importance to follow the evolution of resistance to antibiotics in this species, especially to β-lactams.

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