Effect of pea protein plus grape seed dry extract on a murine model of Candida albicans induced vaginitis

2018 ◽  
Vol 13 (12) ◽  
pp. 1375-1382 ◽  
Author(s):  
Emanuela Esposito ◽  
Michela Campolo ◽  
Giovanna Casili ◽  
Marika Lanza ◽  
Alessia Filippone ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Murine Model ◽  
Grape Seed ◽  
Dry Extract ◽  
Pea Protein

Murine Model of Concurrent Oral and Vaginal Candida albicans Colonisation

2012 ◽  
pp. 527-535 ◽  
Author(s):  
Durdana Rahman ◽  
Mukesh Mistry ◽  
Selvam Thavaraj ◽  
Julian R. Naglik ◽  
Stephen J. Challacombe
Keyword(s):  
Candida Albicans ◽  
Murine Model

Impact of serial systemic infection on Candida albicans virulence factors

2020 ◽  
Vol 15 (13) ◽  
pp. 1249-1263
Author(s):  
Glaucia S Arita ◽  
Daniella R Faria ◽  
Karina M Sakita ◽  
Franciele AV Rodrigues-Vendramini ◽  
Isis RG Capoci ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Virulence Factors ◽  
Murine Model ◽  
Systemic Infection ◽  
Systemic Candidiasis ◽  
Fungal Burden ◽  
Histopathological Findings ◽  
Phenotypic Profile ◽  
Proteomic Data ◽  
Repeated Contact

Aim: To evaluate changes in virulence and pathogenicity approaches from Candida albicans after successive passages in a murine model of systemic candidiasis. Materials & methods: Phenotypic assays were performed using colonies recovered from animals infected serially, totalizing five passages. Results: A progressive infection was observed along the passages, with increased fungal burden and the presence of greater inflammatory areas in the histopathological findings. Recovered strains exhibited increased filamentation and biofilm abilities, along with modulation of phospholipase and proteinase activities. Conclusion: Repeated contact between yeast and host increased the expression of virulence factors. Furthermore, a correspondence between phenotypic profile and proteomic data obtained previously was observed.

scholarly journals Limited Role of Secreted Aspartyl Proteinases Sap1 to Sap6 in Candida albicans Virulence and Host Immune Response in Murine Hematogenously Disseminated Candidiasis

2010 ◽  
Vol 78 (11) ◽  
pp. 4839-4849 ◽  
Author(s):  
Alexandra Correia ◽  
Ulrich Lermann ◽  
Luzia Teixeira ◽  
Filipe Cerca ◽  
Sofia Botelho ◽  
...  
Keyword(s):  
Immune Response ◽  
Candida Albicans ◽  
Significant Role ◽  
Murine Model ◽  
Null Mutant ◽  
Wild Type ◽  
Survival Times ◽  
Disseminated Candidiasis ◽  
Mutant Strains ◽  
Aspartyl Proteinases

ABSTRACTCandida albicanssecreted aspartyl proteinases (Saps) are considered virulence-associated factors. Several members of the Sap family were claimed to play a significant role in the progression of candidiasis established by the hematogenous route. This assumption was based on the observed attenuated virulence ofsap-null mutant strains. However, the exclusive contribution ofSAPgenes to their attenuated phenotype was not unequivocally confirmed, as the Ura status of these mutant strains could also have contributed to the attenuation. In this study, we have reassessed the importance ofSAP1toSAP6in a murine model of hematogenously disseminated candidiasis usingsap-null mutant strains not affected in theirURA3gene expression and compared their virulence phenotypes with those of Ura-blastersapmutants. The median survival time of BALB/c mice intravenously infected with a mutant strain lackingSAP1toSAP3was equivalent to that of mice infected with wild-type strain SC5314, while those infected with mutant strains lackingSAP5showed slightly extended survival times. Nevertheless, no differences could be observed between the wild type and a Δsap456mutant in their abilities to invade mouse kidneys. Likewise, a deficiency inSAP4toSAP6had no noticeable impact on the immune response elicited in the spleens and kidneys ofC. albicans-infected mice. These results contrast with the behavior of equivalent Ura-blaster mutants, which presented a significant reduction in virulence. Our results suggest that Sap1 to Sap6 do not play a significant role inC. albicansvirulence in a murine model of hematogenously disseminated candidiasis and that, in this model, Sap1 to Sap3 are not necessary for successfulC. albicansinfection.

Effect of a Medical Device containing Xyloglucan and Pea Protein on a Murine Model of Atopical Dermatitis.

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Michela Campolo ◽  
Irene Paterniti ◽  
Giovanna Casili ◽  
Alessia Filippone ◽  
Marika lanza ◽  
...  
Keyword(s):  
Medical Device ◽  
Murine Model ◽  
Pea Protein

scholarly journals Efficacies of Fluconazole, Caspofungin, and Amphotericin B in Candida glabrata-Infected p47phox−/− Knockout Mice

2002 ◽  
Vol 46 (5) ◽  
pp. 1240-1245 ◽  
Author(s):  
Justina Y. Ju ◽  
Cynthia Polhamus ◽  
Kieren A. Marr ◽  
Steven M. Holland ◽  
John E. Bennett
Keyword(s):  
Candida Albicans ◽  
Amphotericin B ◽  
Murine Model ◽  
Knockout Mice ◽  
Candida Glabrata ◽  
Drug Efficacy ◽  
Fungal Burden ◽  
Lethal Infection

ABSTRACT Candida glabrata is the second leading cause of adult candidemia, resulting in high mortality. Amphotericin B is considered the treatment of choice, while the efficacy of fluconazole is controversial and caspofungin efficacy is unknown. To ascertain drug efficacy in vivo, the utility of a murine model of C. glabrata infection was investigated. C. glabrata was found to cause progressive, lethal infection when injected intravenously into C57BL/6 mice with reduced oxidative microbicidal capacity due to knockout of the p47phox gene. Spleen and kidney organ CFU counts were determined in groups of mice 2 days after the mice completed 6 days of daily intraperitoneal drug treatment, which began on the day of infection. Daily injections of fluconazole at 80 mg/kg did not reduce spleen or kidney CFU counts after infection with C. glabrata strains having in vitro fluconazole MICs of 2, 32, or 256 μg/ml compared to saline-treated controls. However, this fluconazole regimen reduced spleen CFU counts in mice infected with Candida albicans, an infection that is known to be responsive to fluconazole. Caspofungin at 5 mg/kg and amphotericin B at 5 mg/kg were both effective in reducing fungal burden in spleens and kidneys of C. glabrata-infected mice. Ten mice treated for 6 days with caspofungin at 1 mg/kg survived for 15 days, though all 10 saline-injected mice died or were so ill that they had to be sacrificed by 96 h postinfection. This murine model provided evidence of the efficacy of amphotericin B and caspofungin but not of fluconazole against C. glabrata infection.

Effect of exogenous administration of Candida albicans autoregulatory alcohols in a murine model of hematogenously disseminated candidiasis

2011 ◽  
Vol 52 (4) ◽  
pp. 487-491 ◽  
Author(s):  
Margarida Martins ◽  
Anna L. Lazzell ◽  
Jose L. Lopez-Ribot ◽  
Mariana Henriques ◽  
Rosário Oliveira
Keyword(s):  
Candida Albicans ◽  
Murine Model ◽  
Disseminated Candidiasis

scholarly journals Physiologic Expression of the Candida albicans Pescadillo Homolog Is Required for Virulence in a Murine Model of Hematogenously Disseminated Candidiasis

2012 ◽  
Vol 11 (12) ◽  
pp. 1552-1556 ◽  
Author(s):  
Priya Uppuluri ◽  
Ashok K. Chaturvedi ◽  
Niketa Jani ◽  
Read Pukkila-Worley ◽  
Carlos Monteagudo ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Murine Model ◽  
Critical Role ◽  
Growth Defect ◽  
Mammalian Host ◽  
Developmental Cycle ◽  
Yeast Growth ◽  
Content Type ◽  
Disseminated Candidiasis

ABSTRACT Morphogenetic conversions contribute to the pathogenesis of Candida albicans invasive infections. Many studies to date have convincingly demonstrated a link between filamentation and virulence; however, relatively little is known regarding the role of the filament-to-yeast transition during the pathogenesis of invasive candidiasis. We previously identified the C. albicans pescadillo homolog ( PES1 ) as essential during yeast growth and growth of lateral yeast on hyphae but not during hyphal growth. Furthermore, we demonstrated that PES1 is required for virulence in vivo in a Galleria mellonella larva model of candidiasis. Here, we have used a regulatable tetO-PES1 / pes1 strain to assess the contribution of C. albicans PES1 to pathogenesis in the commonly used and clinically relevant murine model of hematogenously disseminated candidiasis. Our results indicate that a physiologically controlled level of PES1 expression is required for full virulence in this animal model, with virulence defects observed both when PES1 is overexpressed and and when it is depleted. The pathogenetic defect of cells depleted of PES1 is not due to a general growth defect, as demonstrated by the fact that PES1 -depleted cells still kill Caenorhabditis elegans as efficiently as the wild type due to hyphal outgrowth through worm tissues. Our results suggest a critical role of lateral yeast growth in the ability of C. albicans to normally proliferate within tissues, as well as a pivotal role for Pes1 in the normal developmental cycle of C. albicans within the mammalian host during infection.

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