scholarly journals Mass Culturing of Stem and Bulb Nematode (Ditylenchus dipsaci) for use in screenİng and İmpression Training on Carrot Discs

2017 ◽  
Vol 2 (6) ◽  
pp. 3148-3150
Author(s):  
Tohid Behmand ◽  
Lerzan Ozturk ◽  
İbrahim Halil Elekcioğlu
Keyword(s):  
Ditylenchus Dipsaci ◽  
Stem And Bulb Nematode

Validation of extraction methods for diagnosis of the stem and bulb nematode Ditylenchus dipsaci

2019 ◽  
Vol 41 (4) ◽  
pp. 597-602
Author(s):  
Sandra Poirier ◽  
Nathalie Dauphinais ◽  
Guy Bélair ◽  
Valérie Gravel ◽  
Benjamin Mimee
Keyword(s):  
Extraction Methods ◽  
Ditylenchus Dipsaci ◽  
Stem And Bulb Nematode

scholarly journals First Report of Stem and Bulb Nematode Ditylenchus dipsaci on Garlic in New Mexico

2017 ◽  
Vol 18 (2) ◽  
pp. 91-92
Author(s):  
Jason M. French ◽  
Jacki Beacham ◽  
Amanda Garcia ◽  
Natalie P. Goldberg ◽  
Stephen H. Thomas ◽  
...  
Keyword(s):  
International Trade ◽  
New Mexico ◽  
Sequence Data ◽  
The State ◽  
Economic Losses ◽  
First Report ◽  
Important Pest ◽  
Ditylenchus Dipsaci ◽  
Production Areas ◽  
Stem And Bulb Nematode

Taken together, symptoms present, microscopic characterization, and ITS-1 sequence data indicate New Mexico garlic samples infested with Ditylenchus dipsaci, making this the first known report of this pest in the state. This discovery is significant because D. dipsaci can be a persistent pest and has the potential to cause significant economic losses on agronomically important hosts including onion, garlic, and alfalfa. Its longevity in the soil and international trade issues will be concerns for producers. Monitoring of production areas in the region will be performed to determine if this was an isolated and contained introduction or if this important pest has become established in New Mexico.

scholarly journals Discovery of Potato Rot Nematode, Ditylenchus destructor, Infesting Garlic in Ontario, Canada

Plant Disease ◽  
2012 ◽  
Vol 96 (2) ◽  
pp. 297-297 ◽  
Author(s):  
Q. Yu ◽  
M. A. Zaida ◽  
B. Hughes ◽  
M. Celetti
Keyword(s):  
Prince Edward Island ◽  
The Body ◽  
Pcr Analysis ◽  
Suspected Case ◽  
Morphological Studies ◽  
Quarantine Species ◽  
Infested Field ◽  
Ditylenchus Dipsaci ◽  
Ditylenchus Destructor ◽  
Stem And Bulb Nematode

Potato rot nematode, Ditylenchus destructor Thorne, 1945, is a serious nematode pest in a number of root and tube crops, primarily in potatoes, and is an internationally quarantined pest (2). In garlic, it was only reported in Japan (3). In 2011, a survey was conducted for the stem and bulb nematode, Ditylenchus dipsaci (Kühn) Filipjev in Ontario, Canada. PCR analysis revealed that a population of Ditylenchus from one garlic (Allium sativum) bulb sample in a 0.81-ha field (2-acre) in the Ottawa area had a significantly longer ITS1 (approximately 100 bp) than that of D. dipsaci. Subsequent morphology and DNA sequencing concluded that the population was Ditylenchus destructor, a quarantine species in Canada. Twenty females and twenty males were fixed and permanently mounted for morphological studies. The main diagnostic characters matched the description of D. destructor by Thorne (4). The female stylets had strong knobs, 11.1 ± 0.9 (10 to 12) μm long, lateral fields with six distinct lines in the middle section of the body, excretory pore 4.7 ± 1.3 (3 to 6) μm posterior to the hemizonid, esophageal base bulb overlaps dorsally with the intestine, post-vulval uterine sac extends up to two-thirds of the vulva-anus distance, and tail terminus finely rounded. A total of 20 nematode individuals were used for the molecular studies. They had 5-bp differences with the ITS1 (GenBank Accession No. EF208210; 650 bp) sequence of an isolate from Sihong County, China. The sequence of the partial 18S gene (GenBank Accession No. AY593912; 864 bp) was identical to the sequence of an isolate of D. destructor in the Netherlands. The detection of stem and bulb nematode was also confirmed from the field. Infested garlic plants were stunted and the basal bulbs became dark and somewhat rotten. Prior to this finding in Canada, D. destructor was only reported in a few isolated locations in the province of Prince Edward Island (PEI) in 1946 (1). The nematodes in PEI were effectively controlled. Another suspected case in British Columbia was not confirmed since the infested field had been known to be infested with D. dipsaci. To our knowledge, this is the first report of D. destructor on the mainland of Canada. The infested field has been subjected to strict quarantine measures to prevent the spread of the pest. Reference: (1) A. D. Baker et al. Sci. Agric. 26:138, 1946. (2) EPPO. No. 123. Data Sheets on Quarantined Organisms. Bulletin OEPP/EPPO Bull. 8, 1978. (3) T. Fujimura et al. Jpn. J. Nematol. 8:22, 1989. (4) G. Thorne. Proc. Helminthol. Soc. Wash. 12:27, 1945.

scholarly journals Host range and growth of Stem and Bulb Nematode (Ditylenchus dipsaci) populations isolated from garlic and chicory

2010 ◽  
Vol 41 (No. 3) ◽  
pp. 104-108 ◽  
Author(s):  
O. Douda
Keyword(s):  
Life Cycle ◽  
Host Range ◽  
Host Plants ◽  
Dry Weight ◽  
Nematode Infection ◽  
Original Host ◽  
Population Survival ◽  
Ditylenchus Dipsaci ◽  
Stem And Bulb Nematode

Host range and growth of two <I>Ditylenchus dipsaci</I> (Stem and Bulb Nematode) populations on onion, garlic, leek, spinach and chicory were compared. Inoculation was conducted by placing a droplet of 1.5% CMC suspension containing nematodes between the first leaves. The life cycle of the parasite was completed in variants of the original host plants, i.e. salad chicory was infected with the chicory population, and garlic and onion with the garlic population. Survival of the nematodes on garlic and spinach inoculated with the chicory population, and on chicory inoculated with the garlic population was determined. Nematodes of the chicory population did not survive on onion and leek, nor did the garlic population survive on spinach and leek. Nematode infection did not affect the dry weight of aboveground parts of plants, although a reduction would probably occur if the experiments had lasted longer. Symptoms of the infections were apparent in chicory inoculated with the chicory population. Nematodes in the substrate in which the plants were grown were found only in those variants with plants in which the life cycle was completed.

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