scholarly journals Development and validation of a bovine parainfluenza virus type 3 indirect ELISA

2021 ◽  
Vol 6 (6) ◽  
pp. 196-205
Author(s):  
Silvina Soledad Maidana ◽  
Maria Mercedes Odeon ◽  
Carola Maria Ferrecio ◽  
Noelia Magali Grazziotto ◽  
Eddie Pisano ◽  
...  
Keyword(s):  
Virus Type ◽  
Indirect Elisa ◽  
Parainfluenza Virus ◽  
Development And Validation ◽  
Type 3 ◽  
Bovine Parainfluenza Virus

scholarly journals Development and validation of a bovine parainfluenza virus type 3 indirect ELISA

2021 ◽  
Vol 6 (6) ◽  
pp. 196-205
Author(s):  
Silvina Soledad Maidana ◽  
Maria Mercedes Odeon ◽  
Carola Maria Ferrecio ◽  
Noelia Magali Grazziotto ◽  
Eddie Pisano ◽  
...  
Keyword(s):  
Virus Type ◽  
Indirect Elisa ◽  
Parainfluenza Virus ◽  
Development And Validation ◽  
Type 3 ◽  
Bovine Parainfluenza Virus

scholarly journals Quantitative Proteomic Analysis Shows Involvement of the p38 MAPK Pathway in Bovine Parainfluenza Virus type 3 Replication

2021 ◽  
Author(s):  
Liyang Li ◽  
Pengfei Li ◽  
Ao Chen ◽  
Hanbing Li ◽  
Zhe Liu ◽  
...  
Keyword(s):  
Immune Response ◽  
Respiratory Disease ◽  
P38 Mapk ◽  
Virus Type ◽  
Proteomic Analysis ◽  
Parainfluenza Virus ◽  
Bovine Respiratory Disease Complex ◽  
Mdbk Cells ◽  
Type 3 ◽  
Bovine Parainfluenza Virus

Abstract Background: Bovine parainfluenza virus type 3 (BPIV3) infection often causes respiratory tissue damage and immunosuppression and results in bovine respiratory disease complex. Bovine respiratory disease complex is one of the major diseases in dairy cattle and causes huge economical losses every year. The pathogenetic and immunoregulatory mechanisms involved in the process of BPIV3 infection, however, remain unknown. Proteomics is a powerful tool for high-throughput identification of proteins and has been widely used to understand how viruses interact with host cells.Methods: In the present study, we report a proteomic analysis to investigate the whole cellular protein alterations of MDBK cells infected with BPIV3. To investigate the infection process of BPIV3 and the immune response mechanism of MDBK cells, isobaric tags for relative and absolute quantitation analysis (iTRAQ) and Q-Exactive mass spectrometry-based proteomics were performed. The differentially expressed proteins (DEPs) involved in the BPIV3 invasion process in MDBK cells were identified, annotated, and quantitated.Results: A total of 116 proteins, which included 74 upregulated proteins and 42 downregulated proteins, were identified as DEPs between the BPIV3-infected and the mock-infected groups. These DEPs included corresponding proteins related to inflammatory response, immune response, and lipid metabolism. These results might provide some insights for understanding the pathogenesis of BPIV3. Fluorescent quantitative PCR and western blotting analysis showed results consistent with those of iTRAQ identification. Interestingly, the upregulated protein MKK3 was associated with the p38 MAPK signaling pathway.Conclusions: The results of proteomics analysis indicated BPIV3 infection could activate the p38 MAPKpathway to promote virus replication.

scholarly journals Isolation of a Divergent Strain of Bovine Parainfluenza Virus Type 3 (BPIV3) Infecting Cattle in China

Viruses ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 489 ◽  
Author(s):  
Élcio Leal ◽  
Cun Liu ◽  
Zhanzhong Zhao ◽  
Yong Deng ◽  
Fabiola Villanova ◽  
...  
Keyword(s):  
Virus Type ◽  
Immune Escape ◽  
Parainfluenza Virus ◽  
Chinese Isolate ◽  
Respiratory Pathogens ◽  
Adult Cattle ◽  
Mdbk Cells ◽  
Type 3 ◽  
Bovine Parainfluenza Virus ◽  
Genotype A

Bovine parainfluenza virus type 3 (BPIV3) is one of the most important known viral respiratory pathogens of both young and adult cattle. It is also named “heat stress in transport”, causing morbidity and mass death. New variants of BPIV3 have been detected or isolated in China since 2008. Here, we isolate one BPIV3 strain (named BPIV3 BJ) in Madin-Darby bovine kidney (MDBK) cells from nasal samples collected in China. Phylogenetic analysis showed that our isolate is related to BPIV3 of the genotype A. The comparison of BPIV3-BJ and the reference Chinese isolate NM09 showed that these strains are highly divergent. We found many differences in the amino acid composition in the nucleocapsid (NP) protein among these genotype A strains. Since the NP protein has been implicated in immunization studies, our BPIV3 isolate will be useful for the development of immune assays and vaccine studies. The diversity of BPIV3 lineages that we found in China indicated ongoing evolution for immune escape. Our study highlights the importance of genetic surveillance for determining the effect of BPIV3 variability on pathogen evolution and population-scale immunity.

Phylogenetic and antigenic analysis of bovine parainfluenza virus type 3 isolated in Japan between 2002 and 2019

2020 ◽  
Vol 247 ◽  
pp. 108774
Author(s):  
Asuka Kumagai ◽  
Toru Kanno ◽  
Kyoko Kawauchi ◽  
Katsuki Tanaka ◽  
Ryoko Ishihara ◽  
...  
Keyword(s):  
Virus Type ◽  
Parainfluenza Virus ◽  
Antigenic Analysis ◽  
Type 3 ◽  
Bovine Parainfluenza Virus

Antigenic variations of human and bovine parainfluenza virus type 3 strains

1988 ◽  
Vol 11 ◽  
pp. 58
Author(s):  
Ewa Klippmark ◽  
Robert Rydbeck ◽  
Erling Norrby
Keyword(s):  
Virus Type ◽  
Parainfluenza Virus ◽  
Type 3 ◽  
Bovine Parainfluenza Virus
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