scholarly journals Optimized recombinant Bacillus Subtilis 168 whole-cell catalyzes one-step biosynthesis of high fructose syrup

2021 ◽  
Vol 6 (2) ◽  
pp. 067-075
Author(s):  
Ildephonse Habimana ◽  
Qiao Zhina ◽  
Aqeel Sahibzada Muhammad ◽  
Jean Damascene Harindintwali ◽  
Al-Adeeb Abdulquader ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Whole Cell ◽  
Bacillus Subtilis 168 ◽  
High Fructose ◽  
One Step ◽  
High Fructose Syrup ◽  
Recombinant Bacillus Subtilis

scholarly journals Engineering Bacillus Subtilis for the production of High Fructose Syrup: Opportunities and Prospects

2021 ◽  
Vol 6 (2) ◽  
pp. 101-109
Author(s):  
Ildephonse Habimana ◽  
Qiao Zhina ◽  
Aqeel Sahibzada Muhammad ◽  
Jean Damascene Harindintwali ◽  
Abdulqader Al-Adeeb ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
High Fructose ◽  
High Fructose Syrup

Exponential fed-batch strategy for enhancing biosurfactant production by Bacillus subtilis

2014 ◽  
Vol 70 (2) ◽  
pp. 234-240 ◽  
Author(s):  
G. A. Amin
Keyword(s):  
Bacillus Subtilis ◽  
Corn Starch ◽  
Cost Effective ◽  
Batch Process ◽  
Fed Batch ◽  
Cell Biomass ◽  
High Fructose ◽  
Fed Batch Fermentation ◽  
High Fructose Syrup ◽  
Surfactin Production

Surfactin produced by Bacillus subtilis BDCC-TUSA-3 from Maldex-15 was used as a growth-associated product in a conventional batch process. Maldex-15 is a cheap industrial by-product recovered during manufacturing of high fructose syrup from corn starch. Surfactin production was greatly improved in exponential fed-batch fermentation. Maldex-15 and other nutrients were exponentially fed into the culture based on the specific growth rate of the bacterium. In order to maximize surfactin yield and productivity, conversion of different quantities of Maldex-15 into surfactin was investigated in five different fermentation runs. In all runs, most of the Maldex-15 was consumed and converted into surfactin and cell biomass with appreciable efficiencies. The best results were obtained with the fermentation run supplied with 204 g Maldex-15. Up to 36.1 g l−1 of surfactin and cell biomass of 31.8 g l−1 were achieved in 12 h. Also, a marked substrate yield of 0.272 g g−1 and volumetric reactor productivity of 2.58 g 1−1 h−1 were obtained, confirming the establishment of a cost-effective commercial surfactin production.

scholarly journals Production of d-Tagatose by Whole-Cell Conversion of Recombinant Bacillus subtilis in the Absence of Antibiotics

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1343
Author(s):  
Xian Zhang ◽  
Ruiqi Lu ◽  
Qiang Wang ◽  
Mengkai Hu ◽  
Zhiyue Li ◽  
...  
Keyword(s):  
Heterologous Gene Expression ◽  
Maximum Yield ◽  
Alanine Racemase ◽  
Lacz Gene ◽  
Whole Cell ◽  
Cell Conversion ◽  
One Step ◽  
Overlapping Extension Pcr ◽  
Recombinant Bacillus Subtilis ◽  
Arabinose Isomerase

d-tagatose is a popular functional monosaccharide produced from lactose by β-galactosidase and arabinose isomerase. In this study, two d-alanine-deficient heterologous gene expression systems were constructed, B. subtilis 168 D1 and B. subtilis 168 D2, using overlapping extension PCR and the CRE/loxP system. The lacZ gene for β-galactosidase was integrated into a specific locus of the chassis B. subtilis 168 D2. A mutually complementary plasmid pMA5 with the alanine racemase gene alrA attached to it was constructed and used to assemble recombinant plasmids overexpressing β-galactosidase and arabinose isomerase. Afterward, an integrated recombinant was constructed by the plasmid expressing the arabinose isomerase gene araA of E. coli transform-competent B. subtilis 168 D2 cells. The co-expressing plasmids were introduced into alanine racemase knockout B. subtilis 168 D1. Whole-cell bioconversion was performed using the integrated recombinant with a maximum yield of 96.8 g/L d-tagatose from 500 g/L lactose, and the highest molar conversions were 57.2%. B. subtilis 168 D1/pMA5-alrA-araA-lacZ is capable of single-cell one-step production of d-tagatose. This study provides a new approach to the production of functional sugars.

Sign in / Sign up

Export Citation Format

Share Document